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P30051 (TEAD1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 113. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Transcriptional enhancer factor TEF-1
Alternative name(s):
NTEF-1
Protein GT-IIC
TEA domain family member 1
Short name=TEAD-1
Transcription factor 13
Short name=TCF-13
Gene names
Name:Tead1
Synonyms:Tcf13, Tef-1, Tef1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length426 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds specifically and cooperatively to the SPH and GT-IIC 'enhansons' (5'-GTGGAATGT-3') and activates transcription in vivo in a cell-specific manner. The activation function appears to be mediated by a limiting cell-specific transcriptional intermediary factor (TIF). Involved in cardiac development. Binds to the M-CAT motif By similarity.

Subunit structure

Interacts with YAP1 and WWTR1/TAZ By similarity.

Subcellular location

Nucleus.

Tissue specificity

In developing skeletal muscle and myocardium, in mitotic neuroblasts both in the brain and spinal cord. At later stages of embryogenesis expressed in several developing structures such as the olfactory system, the intestine, and the kidney.

Sequence similarities

Contains 1 TEA DNA-binding domain.

Caution

It is uncertain whether Met-1 or Met-16 is the initiator.

Sequence caution

The sequence AAA40410.1 differs from that shown. Reason: Unusual initiator. The initiator methionine is coded by a non-canonical ATT isoleucine codon.

The sequence AAA40411.1 differs from that shown. Reason: Unusual initiator. The initiator methionine is coded by a non-canonical ATT isoleucine codon.

The sequence AAB32420.1 differs from that shown. Reason: Unusual initiator. The initiator methionine is coded by a non-canonical ATT isoleucine codon.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   LigandDNA-binding
   Molecular functionActivator
   PTMAcetylation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processembryonic heart tube morphogenesis

Inferred from genetic interaction PubMed 18332127. Source: MGI

heart development

Inferred from mutant phenotype Ref.3. Source: MGI

hippo signaling

Inferred from genetic interaction PubMed 19004856. Source: MGI

lateral mesoderm development

Inferred from genetic interaction PubMed 18332127. Source: MGI

notochord development

Inferred from genetic interaction PubMed 18332127. Source: MGI

paraxial mesoderm development

Inferred from genetic interaction PubMed 18332127. Source: MGI

positive regulation of transcription from RNA polymerase II promoter

Inferred from genetic interaction PubMed 16207754. Source: MGI

regulation of cell proliferation

Inferred from genetic interaction PubMed 19004856. Source: MGI

regulation of transcription, DNA-templated

Inferred from direct assay PubMed 14762206. Source: MGI

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentnucleus

Inferred from direct assay PubMed 19004856. Source: MGI

transcription factor complex

Inferred from direct assay PubMed 16207754. Source: MGI

   Molecular_functionDNA binding

Inferred from direct assay PubMed 11306707PubMed 14762206PubMed 16207754. Source: MGI

protein binding

Inferred from physical interaction PubMed 14762206PubMed 19952108. Source: MGI

sequence-specific DNA binding transcription factor activity

Inferred from direct assay PubMed 14762206. Source: MGI

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 426426Transcriptional enhancer factor TEF-1
PRO_0000205931

Regions

DNA binding30 – 9768TEA
Region167 – 426260Transcriptional activation Potential
Compositional bias143 – 20462Pro-rich

Amino acid modifications

Modified residue11N-acetylmethionine By similarity

Experimental info

Sequence conflict9 – 102SE → RR in AAB32420. Ref.3
Sequence conflict1101K → KVTSM in AAA40411. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P30051 [UniParc].

Last modified July 15, 1998. Version 2.
Checksum: AEC83DAD2DDAF07A

FASTA42647,948
        10         20         30         40         50         60 
MEPSSWSGSE SPAENMERMS DSADKPIDND AEGVWSPDIE QSFQEALAIY PPCGRRKIIL 

        70         80         90        100        110        120 
SDEGKMYGRN ELIARYIKLR TGKTRTRKQV SSHIQVLARR KSRDFHSKLK DQTAKDKALQ 

       130        140        150        160        170        180 
HMAAMSSAQI VSATAIHNKL GLPGIPRPTF PGGPGFWPGM IQTGQPGSSQ DVKPFVQQAY 

       190        200        210        220        230        240 
PIQPAVTAPI PGFEPTSAPA PSVPAWQGRS IGTTKLRLVE FSAFLEQQRD PDSYNKHLFV 

       250        260        270        280        290        300 
HIGHANHSYS DPLLESVDIR QIYDKFPEKK GGLKELFGKG PQNAFFLVKF WADLNCNIQD 

       310        320        330        340        350        360 
DAGAFYGVSS QYESSENMTV TCSTKVCSFG KQVVEKVETE YARFENGRFV YRINRSPMCE 

       370        380        390        400        410        420 
YMINFIHKLK HLPEKYMMNS VLENFTILLV VTNRDTQETL LCMACVFEVS NSEHGAQHHI 


YRLVKD 

« Hide

References

[1]"Striking homology between mouse and human transcription enhancer factor-1 (TEF-1)."
Blatt C., Depamphilis M.L.
Nucleic Acids Res. 21:747-748(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Both a ubiquitous factor mTEF-1 and a distinct muscle-specific factor bind to the M-CAT motif of the myosin heavy chain beta gene."
Shimizu N.N., Smith G., Izumo S.
Nucleic Acids Res. 21:4103-4110(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: BALB/c.
Tissue: Heart.
[3]"Transcriptional enhancer factor 1 disruption by a retroviral gene trap leads to heart defects and embryonic lethality in mice."
Chen Z., Friedrich G.A., Soriano P.
Genes Dev. 8:2293-2301(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L06865 mRNA. Translation: AAA40410.1. Sequence problems.
L13853 mRNA. Translation: AAA40411.1. Sequence problems.
S74227 mRNA. Translation: AAB32420.1. Sequence problems.
PIRA54882.
S40779.
S41767.
RefSeqNP_033372.1. NM_009346.3.
UniGeneMm.24685.
Mm.398985.

3D structure databases

ProteinModelPortalP30051.
SMRP30051. Positions 27-105, 209-425.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid204098. 3 interactions.
DIPDIP-41748N.
IntActP30051. 2 interactions.
MINTMINT-1514648.

PTM databases

PhosphoSiteP30051.

Proteomic databases

PaxDbP30051.
PRIDEP30051.

Protocols and materials databases

DNASU21676.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID21676.
KEGGmmu:21676.
UCSCuc009jgz.2. mouse.

Organism-specific databases

CTD7003.
MGIMGI:101876. Tead1.

Phylogenomic databases

eggNOGNOG246992.
HOGENOMHOG000253933.
HOVERGENHBG056905.
KOK09448.
PhylomeDBP30051.

Gene expression databases

ArrayExpressP30051.
BgeeP30051.
CleanExMM_TEAD1.
GenevestigatorP30051.

Family and domain databases

InterProIPR000818. TEA/ATTS.
IPR016361. TEF.
[Graphical view]
PANTHERPTHR11834. PTHR11834. 1 hit.
PfamPF01285. TEA. 1 hit.
[Graphical view]
PIRSFPIRSF002603. TEF. 1 hit.
PRINTSPR00065. TEADOMAIN.
SMARTSM00426. TEA. 1 hit.
[Graphical view]
PROSITEPS00554. TEA_1. 1 hit.
PS51088. TEA_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSTEAD1. mouse.
NextBio300968.
PROP30051.
SOURCESearch...

Entry information

Entry nameTEAD1_MOUSE
AccessionPrimary (citable) accession number: P30051
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: July 15, 1998
Last modified: July 9, 2014
This is version 113 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot