P30051 (TEAD1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcriptional enhancer factor TEF-1

Alternative name(s):
NTEF-1
Protein GT-IIC
TEA domain family member 1
Short name=TEAD-1
Transcription factor 13
Short name=TCF-13

Gene names

Name:	Tead1
Synonyms:	Tcf13, Tef-1, Tef1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	426 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds specifically and cooperatively to the SPH and GT-IIC 'enhansons' (5'-GTGGAATGT-3') and activates transcription in vivo in a cell-specific manner. The activation function appears to be mediated by a limiting cell-specific transcriptional intermediary factor (TIF). Involved in cardiac development. Binds to the M-CAT motif By similarity.
Subunit structure	Interacts with YAP1 and WWTR1/TAZ By similarity.
Subcellular location	Nucleus.
Tissue specificity	In developing skeletal muscle and myocardium, in mitotic neuroblasts both in the brain and spinal cord. At later stages of embryogenesis expressed in several developing structures such as the olfactory system, the intestine, and the kidney.
Sequence similarities	Contains 1 TEA DNA-binding domain.
Caution	It is uncertain whether Met-1 or Met-16 is the initiator.
Sequence caution	The sequence AAA40410.1 differs from that shown. Reason: Unusual initiator. The initiator methionine is coded by a non-canonical ATT isoleucine codon. The sequence AAA40411.1 differs from that shown. Reason: Unusual initiator. The initiator methionine is coded by a non-canonical ATT isoleucine codon. The sequence AAB32420.1 differs from that shown. Reason: Unusual initiator. The initiator methionine is coded by a non-canonical ATT isoleucine codon.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Ligand	DNA-binding
Molecular function	Activator
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	heart development Inferred from mutant phenotype Ref.3. Source: MGI hippo signaling cascade Inferred from genetic interaction. Source: MGI positive regulation of transcription from RNA polymerase II promoter Inferred from genetic interaction. Source: MGI regulation of cell proliferation Inferred from genetic interaction. Source: MGI transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	transcription factor complex Inferred from direct assay. Source: MGI
Molecular function	DNA binding Inferred from direct assay. Source: MGI protein binding Inferred from physical interaction. Source: MGI sequence-specific DNA binding transcription factor activity Inferred from direct assay. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 426	426	Transcriptional enhancer factor TEF-1		PRO_0000205931
Regions
	DNA binding	30 – 97	68	TEA
	Region	167 – 426	260	Transcriptional activation Potential
	Compositional bias	143 – 204	62	Pro-rich
Amino acid modifications
	Modified residue	7	1	Phosphoserine By similarity
	Modified residue	9	1	Phosphoserine By similarity
	Modified residue	11	1	Phosphoserine By similarity
Experimental info
	Sequence conflict	9 – 10	2	SE → RR in AAB32420. Ref.3
	Sequence conflict	110	1	K → KVTSM in AAA40411. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P30051 [UniParc]. Last modified July 15, 1998. Version 2. Checksum: AEC83DAD2DDAF07A	FASTA	426	47,948
	10 20 30 40 50 60 MEPSSWSGSE SPAENMERMS DSADKPIDND AEGVWSPDIE QSFQEALAIY PPCGRRKIIL 70 80 90 100 110 120 SDEGKMYGRN ELIARYIKLR TGKTRTRKQV SSHIQVLARR KSRDFHSKLK DQTAKDKALQ 130 140 150 160 170 180 HMAAMSSAQI VSATAIHNKL GLPGIPRPTF PGGPGFWPGM IQTGQPGSSQ DVKPFVQQAY 190 200 210 220 230 240 PIQPAVTAPI PGFEPTSAPA PSVPAWQGRS IGTTKLRLVE FSAFLEQQRD PDSYNKHLFV 250 260 270 280 290 300 HIGHANHSYS DPLLESVDIR QIYDKFPEKK GGLKELFGKG PQNAFFLVKF WADLNCNIQD 310 320 330 340 350 360 DAGAFYGVSS QYESSENMTV TCSTKVCSFG KQVVEKVETE YARFENGRFV YRINRSPMCE 370 380 390 400 410 420 YMINFIHKLK HLPEKYMMNS VLENFTILLV VTNRDTQETL LCMACVFEVS NSEHGAQHHI YRLVKD

References

[1]	"Striking homology between mouse and human transcription enhancer factor-1 (TEF-1)." Blatt C., Depamphilis M.L. Nucleic Acids Res. 21:747-748(1993) [PubMed: 8441689] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Both a ubiquitous factor mTEF-1 and a distinct muscle-specific factor bind to the M-CAT motif of the myosin heavy chain beta gene." Shimizu N.N., Smith G., Izumo S. Nucleic Acids Res. 21:4103-4110(1993) [PubMed: 8396764] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c. Tissue: Heart.
[3]	"Transcriptional enhancer factor 1 disruption by a retroviral gene trap leads to heart defects and embryonic lethality in mice." Chen Z., Friedrich G.A., Soriano P. Genes Dev. 8:2293-2301(1994) [PubMed: 7958896] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L06865 mRNA. Translation: AAA40410.1. Sequence problems. L13853 mRNA. Translation: AAA40411.1. Sequence problems. S74227 mRNA. Translation: AAB32420.1. Sequence problems.
IPI	IPI00331222.
PIR	A54882. S40779. S41767.
RefSeq	NP_033372.1. NM_009346.3.
UniGene	Mm.24685. Mm.398985.
3D structure databases
ProteinModelPortal	P30051.
SMR	P30051. Positions 27-105, 209-425.
ModBase	Search...
Protein-protein interaction databases
IntAct	P30051. 1 interaction.
STRING	P30051.
PTM databases
PhosphoSite	P30051.
Proteomic databases
PRIDE	P30051.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	21676.
KEGG	mmu:21676.
Organism-specific databases
CTD	7003.
MGI	MGI:101876. Tead1.
Phylogenomic databases
eggNOG	roNOG09290.
HOVERGEN	HBG056905.
Gene expression databases
ArrayExpress	P30051.
Bgee	P30051.
CleanEx	MM_TEAD1.
Genevestigator	P30051.
GermOnline	ENSMUSG00000055320. Mus musculus.
Family and domain databases
InterPro	IPR000818. TEA/ATTS. IPR016361. TEF. [Graphical view]
KO	K09448.
PANTHER	PTHR11834. TEA/ATTS. 1 hit.
Pfam	PF01285. TEA. 1 hit. [Graphical view]
PIRSF	PIRSF002603. TEF. 1 hit.
PRINTS	PR00065. TEADOMAIN.
SMART	SM00426. TEA. 1 hit. [Graphical view]
PROSITE	PS00554. TEA_1. 1 hit. PS51088. TEA_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
SOURCE	Search...

Entry information

Entry name

TEAD1_MOUSE

Accession

Primary (citable) accession number: P30051

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	July 15, 1998
Last modified:	December 14, 2011
This is version 97 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program