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Protein

Transcriptional enhancer factor TEF-1

Gene

Tead1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds specifically and cooperatively to the SPH and GT-IIC 'enhansons' (5'-GTGGAATGT-3') and activates transcription in vivo in a cell-specific manner. The activation function appears to be mediated by a limiting cell-specific transcriptional intermediary factor (TIF). Involved in cardiac development. Binds to the M-CAT motif (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi28 – 104TEAPROSITE-ProRule annotationAdd BLAST77

GO - Molecular functioni

GO - Biological processi

  • cellular response to retinoic acid Source: MGI
  • embryonic heart tube morphogenesis Source: MGI
  • heart development Source: MGI
  • hippo signaling Source: MGI
  • lateral mesoderm development Source: MGI
  • notochord development Source: MGI
  • paraxial mesoderm development Source: MGI
  • positive regulation of pri-miRNA transcription from RNA polymerase II promoter Source: MGI
  • positive regulation of protein serine/threonine kinase activity Source: MGI
  • positive regulation of stem cell population maintenance Source: MGI
  • positive regulation of transcription, DNA-templated Source: MGI
  • positive regulation of transcription from RNA polymerase II promoter Source: MGI
  • protein complex assembly Source: MGI
  • regulation of cell proliferation Source: MGI
  • regulation of transcription, DNA-templated Source: MGI
  • transcription, DNA-templated Source: UniProtKB-KW
  • transition between fast and slow fiber Source: MGI

Keywordsi

Molecular functionActivator, DNA-binding
Biological processTranscription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Transcriptional enhancer factor TEF-1
Alternative name(s):
NTEF-1
Protein GT-IIC
TEA domain family member 1
Short name:
TEAD-1
Transcription factor 13
Short name:
TCF-13
Gene namesi
Name:Tead1
Synonyms:Tcf13, Tef-1, Tef1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:101876. Tead1.

Subcellular locationi

GO - Cellular componenti

  • nucleoplasm Source: MGI
  • nucleus Source: MGI
  • TEAD-1-YAP complex Source: MGI
  • transcription factor complex Source: MGI

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002059311 – 426Transcriptional enhancer factor TEF-1Add BLAST426

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei11PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP30051.
PRIDEiP30051.

PTM databases

iPTMnetiP30051.
PhosphoSitePlusiP30051.
SwissPalmiP30051.

Expressioni

Tissue specificityi

In developing skeletal muscle and myocardium, in mitotic neuroblasts both in the brain and spinal cord. At later stages of embryogenesis expressed in several developing structures such as the olfactory system, the intestine, and the kidney.

Gene expression databases

BgeeiENSMUSG00000055320.
CleanExiMM_TEAD1.

Interactioni

Subunit structurei

Interacts with YAP1 and WWTR1/TAZ.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Yap1P469382EBI-3953905,EBI-1211949

GO - Molecular functioni

Protein-protein interaction databases

BioGridi204098. 3 interactors.
DIPiDIP-41748N.
IntActiP30051. 2 interactors.
MINTiMINT-1514648.

Structurei

3D structure databases

ProteinModelPortaliP30051.
SMRiP30051.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni167 – 426Transcriptional activationSequence analysisAdd BLAST260

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi143 – 204Pro-richAdd BLAST62

Phylogenomic databases

HOGENOMiHOG000253933.
HOVERGENiHBG056905.
InParanoidiP30051.
KOiK09448.
PhylomeDBiP30051.

Family and domain databases

InterProiView protein in InterPro
IPR000818. TEA/ATTS_dom.
IPR016361. TEF_metazoa.
PfamiView protein in Pfam
PF01285. TEA. 1 hit.
PIRSFiPIRSF002603. TEF. 1 hit.
PRINTSiPR00065. TEADOMAIN.
SMARTiView protein in SMART
SM00426. TEA. 1 hit.
PROSITEiView protein in PROSITE
PS00554. TEA_1. 1 hit.
PS51088. TEA_2. 1 hit.

Sequencei

Sequence statusi: Complete.

P30051-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEPSSWSGSE SPAENMERMS DSADKPIDND AEGVWSPDIE QSFQEALAIY
60 70 80 90 100
PPCGRRKIIL SDEGKMYGRN ELIARYIKLR TGKTRTRKQV SSHIQVLARR
110 120 130 140 150
KSRDFHSKLK DQTAKDKALQ HMAAMSSAQI VSATAIHNKL GLPGIPRPTF
160 170 180 190 200
PGGPGFWPGM IQTGQPGSSQ DVKPFVQQAY PIQPAVTAPI PGFEPTSAPA
210 220 230 240 250
PSVPAWQGRS IGTTKLRLVE FSAFLEQQRD PDSYNKHLFV HIGHANHSYS
260 270 280 290 300
DPLLESVDIR QIYDKFPEKK GGLKELFGKG PQNAFFLVKF WADLNCNIQD
310 320 330 340 350
DAGAFYGVSS QYESSENMTV TCSTKVCSFG KQVVEKVETE YARFENGRFV
360 370 380 390 400
YRINRSPMCE YMINFIHKLK HLPEKYMMNS VLENFTILLV VTNRDTQETL
410 420
LCMACVFEVS NSEHGAQHHI YRLVKD
Length:426
Mass (Da):47,948
Last modified:July 15, 1998 - v2
Checksum:iAEC83DAD2DDAF07A
GO

Sequence cautioni

The sequence AAA40410 differs from that shown. Unusual initiator. The initiator methionine is coded by a non-canonical ATT isoleucine codon.Curated
The sequence AAA40411 differs from that shown. Unusual initiator. The initiator methionine is coded by a non-canonical ATT isoleucine codon.Curated
The sequence AAB32420 differs from that shown. Unusual initiator. The initiator methionine is coded by a non-canonical ATT isoleucine codon.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti9 – 10SE → RR in AAB32420 (PubMed:7958896).Curated2
Sequence conflicti110K → KVTSM in AAA40411 (PubMed:8396764).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L06865 mRNA. Translation: AAA40410.1. Sequence problems.
L13853 mRNA. Translation: AAA40411.1. Sequence problems.
S74227 mRNA. Translation: AAB32420.1. Sequence problems.
PIRiA54882.
S40779.
S41767.
RefSeqiNP_033372.1. NM_009346.3.
UniGeneiMm.24685.
Mm.398985.

Genome annotation databases

GeneIDi21676.
KEGGimmu:21676.
UCSCiuc009jgz.2. mouse.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiTEAD1_MOUSE
AccessioniPrimary (citable) accession number: P30051
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: July 15, 1998
Last modified: June 7, 2017
This is version 141 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

It is uncertain whether Met-1 or Met-16 is the initiator.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot