P30050 (RL12_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
60S ribosomal protein L12

Gene names

Name:

RPL12

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	165 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Binds directly to 26S ribosomal RNA By similarity.
Sequence similarities	Belongs to the ribosomal protein L11P family.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Ligand	RNA-binding
Molecular function	Ribonucleoprotein Ribosomal protein
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	SRP-dependent cotranslational protein targeting to membrane Traceable author statement. Source: Reactome nuclear-transcribed mRNA catabolic process, nonsense-mediated decay Traceable author statement. Source: Reactome translational elongation Traceable author statement. Source: Reactome translational initiation Traceable author statement. Source: Reactome translational termination Traceable author statement. Source: Reactome viral transcription Traceable author statement. Source: Reactome
Cellular_component	cytosolic large ribosomal subunit Inferred from direct assay PubMed 12962325. Source: UniProtKB
Molecular_function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW structural constituent of ribosome Non-traceable author statement PubMed 12962325. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P30050-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P30050-2)

The sequence of this isoform differs from the canonical sequence as follows:
     38-70: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 165	165	60S ribosomal protein L12		PRO_0000104456
Amino acid modifications
	Modified residue	38	1	Phosphoserine Ref.5 Ref.6 Ref.9 Ref.11
	Modified residue	54	1	N6-acetyllysine Ref.8
	Modified residue	83	1	N6-acetyllysine Ref.8
Natural variations
	Alternative sequence	38 – 70	33	Missing in isoform 2.		VSP_034695

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified April 1, 1993. Version 1. Checksum: 7EFD783A8ED350F9	FASTA	165	17,819
	10 20 30 40 50 60 MPPKFDPNEI KVVYLRCTGG EVGATSALAP KIGPLGLSPK KVGDDIAKAT GDWKGLRITV 70 80 90 100 110 120 KLTIQNRQAQ IEVVPSASAL IIKALKEPPR DRKKQKNIKH SGNITFDEIV NIARQMRHRS 130 140 150 160 LARELSGTIK EILGTAQSVG CNVDGRHPHD IIDDINSGAV ECPAS
	Isoform 2 [UniParc]. Checksum: 1FDF4BEF878AD5D5	FASTA	132	14,199
	10 20 30 40 50 60 MPPKFDPNEI KVVYLRCTGG EVGATSALAP KIGPLGLIEV VPSASALIIK ALKEPPRDRK 70 80 90 100 110 120 KQKNIKHSGN ITFDEIVNIA RQMRHRSLAR ELSGTIKEIL GTAQSVGCNV DGRHPHDIID 130 DINSGAVECP AS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The primary structure of human ribosomal protein L12." Chu W., Presky D.H., Swerlisk R.A., Burns D.K. Nucleic Acids Res. 21:749-749(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"DNA sequence and analysis of human chromosome 9." Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L. , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I. Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Ovary.
[5]	"A probability-based approach for high-throughput protein phosphorylation analysis and site localization." Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P. Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-38, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[6]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-38, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[7]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[8]	"Lysine acetylation targets protein complexes and co-regulates major cellular functions." Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M. Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-54 AND LYS-83, MASS SPECTROMETRY.
[9]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-38, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[10]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]	"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-38, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L06505 mRNA. Translation: AAA36157.1. AL445222 Genomic DNA. Translation: CAH72929.1. CH471090 Genomic DNA. Translation: EAW87669.1. BC050644 mRNA. Translation: AAH50644.1. BC059950 mRNA. Translation: AAH59950.1. BC094831 mRNA. Translation: AAH94831.1. BC105603 mRNA. Translation: AAI05604.1.
IPI	IPI00024933. IPI00816063.
PIR	S35531.
RefSeq	NP_000967.1. NM_000976.3.
UniGene	Hs.408054.
3D structure databases
ProteinModelPortal	P30050.
ModBase	Search...
Protein-protein interaction databases
IntAct	P30050. 22 interactions.
MINT	MINT-2802291.
STRING	9606.ENSP00000354739.
PTM databases
PhosphoSite	P30050.
Polymorphism databases
DMDM	266921.
2D gel databases
SWISS-2DPAGE	P30050.
Proteomic databases
PaxDb	P30050.
PRIDE	P30050.
Protocols and materials databases
DNASU	6136.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000361436; ENSP00000354739; ENSG00000197958. ENST00000536368; ENSP00000441179; ENSG00000197958.
GeneID	6136.
KEGG	hsa:6136.
UCSC	uc004bqy.2. human. uc004bqz.2. human.
Organism-specific databases
CTD	6136.
GeneCards	GC09M130209.
H-InvDB	HIX0170298.
HGNC	HGNC:10302. RPL12.
HPA	HPA003403.
MIM	180475. gene.
neXtProt	NX_P30050.
PharmGKB	PA34665.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG0080.
HOVERGEN	HBG007231.
InParanoid	P30050.
KO	K02870.
OMA	PRSMARE.
OrthoDB	EOG473PSB.
Enzyme and pathway databases
Reactome	REACT_116125. Disease. REACT_17015. Metabolism of proteins. REACT_1762. 3' -UTR-mediated translational regulation. REACT_21257. Metabolism of RNA. REACT_71. Gene Expression.
Gene expression databases
Bgee	P30050.
CleanEx	HS_RPL12.
Genevestigator	P30050.
GermOnline	ENSG00000197958. Homo sapiens.
Family and domain databases
Gene3D	1.10.10.250. 1 hit. 3.30.1550.10. 1 hit.
InterPro	IPR000911. Ribosomal_L11. IPR020783. Ribosomal_L11_C. IPR020785. Ribosomal_L11_CS. IPR020784. Ribosomal_L11_N. [Graphical view]
PANTHER	PTHR11661. PTHR11661. 1 hit.
Pfam	PF00298. Ribosomal_L11. 1 hit. PF03946. Ribosomal_L11_N. 1 hit. [Graphical view]
SMART	SM00649. RL11. 1 hit. [Graphical view]
SUPFAM	SSF46906. Ribosomal_L11. 1 hit. SSF54747. Ribosomal_L11. 1 hit.
PROSITE	PS00359. RIBOSOMAL_L11. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	RPL12. human.
GenomeRNAi	6136.
NextBio	23835.
SOURCE	Search...

Entry information

Entry name

RL12_HUMAN

Accession

Primary (citable) accession number: P30050
Secondary accession number(s): Q5VVV2, Q6PB27

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	May 1, 2013
This is version 127 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.