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P30047 (GFRP_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
GTP cyclohydrolase 1 feedback regulatory protein

Short name=GFRP
Alternative name(s):
GTP cyclohydrolase I feedback regulatory protein
p35
Gene names
Name:GCHFR
Synonyms:GFRP
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length84 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Mediates tetrahydrobiopterin inhibition of GTP cyclohydrolase 1. This inhibition is reversed by L-phenylalanine. Ref.6

Subunit structure

Homopentamer. Forms a complex with GCH1 where a GCH1 homodecamer is sandwiched by two GFRP homopentamers By similarity. Interacts with GCH1. Ref.7

Subcellular location

Nucleus. Nucleus membrane. Cytoplasmcytosol Ref.6.

Tissue specificity

In epidermis, expressed predominantly in basal undifferentiated keratinocytes and in some but not all melanocytes (at protein level). Ref.6

Sequence similarities

Belongs to the GFRP family.

Ontologies

Keywords
   Cellular componentCytoplasm
Membrane
Nucleus
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processnegative regulation of GTP cyclohydrolase I activity

Inferred from electronic annotation. Source: Ensembl

negative regulation of biosynthetic process

Inferred from electronic annotation. Source: InterPro

neurotransmitter metabolic process

Traceable author statement PubMed 8702680. Source: ProtInc

nitric oxide biosynthetic process

Traceable author statement PubMed 8702680. Source: ProtInc

nitric oxide metabolic process

Traceable author statement. Source: Reactome

protein heterooligomerization

Inferred from electronic annotation. Source: Ensembl

regulation of nitric-oxide synthase activity

Traceable author statement. Source: Reactome

small molecule metabolic process

Traceable author statement. Source: Reactome

   Cellular_componentcytoplasm

Inferred from direct assay Ref.6. Source: UniProtKB

cytosol

Traceable author statement. Source: Reactome

dendrite

Inferred from direct assay Ref.6. Source: UniProtKB

melanosome

Inferred from direct assay Ref.6. Source: UniProtKB

nuclear membrane

Inferred from direct assay Ref.6. Source: UniProtKB

nucleus

Inferred from direct assay Ref.6. Source: UniProtKB

protein complex

Inferred from electronic annotation. Source: Ensembl

   Molecular_functionamino acid binding

Inferred from electronic annotation. Source: Ensembl

enzyme inhibitor activity

Inferred from electronic annotation. Source: Ensembl

protein binding

Inferred from physical interaction Ref.7PubMed 9092499. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.5
Chain2 – 8483GTP cyclohydrolase 1 feedback regulatory protein
PRO_0000189675

Sequences

Sequence LengthMass (Da)Tools
P30047 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 4A20FDBC6E3EE7AC

FASTA849,698
        10         20         30         40         50         60 
MPYLLISTQI RMEVGPTMVG DEQSDPELMQ HLGASKRRAL GNNFYEYYVD DPPRIVLDKL 

        70         80 
ERRGFRVLSM TGVGQTLVWC LHKE 

« Hide

References

« Hide 'large scale' references
[1]"Structure of the human GTP cyclohydrolase I feedback regulatory protein gene."
Bonner T.I., Modi W.S., Milstein S.
Submitted (NOV-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Liver.
[3]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Colon.
[5]"Human liver protein map: a reference database established by microsequencing and gel comparison."
Hochstrasser D.F., Frutiger S., Paquet N., Bairoch A., Ravier F., Pasquali C., Sanchez J.-C., Tissot J.-D., Bjellqvist B., Vargas R., Appel R.D., Hughes G.J.
Electrophoresis 13:992-1001(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-12.
Tissue: Liver.
[6]"GTP cyclohydrolase feedback regulatory protein controls cofactor 6-tetrahydrobiopterin synthesis in the cytosol and in the nucleus of epidermal keratinocytes and melanocytes."
Chavan B., Gillbro J.M., Rokos H., Schallreuter K.U.
J. Invest. Dermatol. 126:2481-2489(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[7]"A yeast 2-hybrid analysis of human GTP cyclohydrolase I protein interactions."
Swick L., Kapatos G.
J. Neurochem. 97:1447-1455(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH GCH1.
[8]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U78190 Genomic DNA. Translation: AAB37337.1.
AK311872 mRNA. Translation: BAG34813.1.
CH471125 Genomic DNA. Translation: EAW92443.1.
BC112262 mRNA. Translation: AAI12263.1.
BC112264 mRNA. Translation: AAI12265.1.
CCDSCCDS10064.1.
RefSeqNP_005249.1. NM_005258.2.
UniGeneHs.631717.

3D structure databases

ProteinModelPortalP30047.
SMRP30047. Positions 2-84.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid108914. 3 interactions.
IntActP30047. 1 interaction.
STRING9606.ENSP00000260447.

PTM databases

PhosphoSiteP30047.

Polymorphism databases

DMDM2506906.

2D gel databases

SWISS-2DPAGEP30047.

Proteomic databases

MaxQBP30047.
PaxDbP30047.
PeptideAtlasP30047.
PRIDEP30047.

Protocols and materials databases

DNASU2644.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000260447; ENSP00000260447; ENSG00000137880.
GeneID2644.
KEGGhsa:2644.
UCSCuc001zmr.1. human.

Organism-specific databases

CTD2644.
GeneCardsGC15P041058.
HGNCHGNC:4194. GCHFR.
HPAHPA046258.
MIM602437. gene.
neXtProtNX_P30047.
PharmGKBPA28609.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG41956.
HOGENOMHOG000008074.
HOVERGENHBG000233.
InParanoidP30047.
OrthoDBEOG7N63Q7.
PhylomeDBP30047.
TreeFamTF329303.

Enzyme and pathway databases

ReactomeREACT_111217. Metabolism.

Gene expression databases

ArrayExpressP30047.
BgeeP30047.
CleanExHS_GCHFR.
GenevestigatorP30047.

Family and domain databases

Gene3D3.30.1410.10. 1 hit.
InterProIPR009112. GTP_CycHdrlase_I_reg.
[Graphical view]
PfamPF06399. GFRP. 1 hit.
[Graphical view]
ProDomPD028559. GTP_CycHdrlase_I_reg. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMSSF69761. SSF69761. 1 hit.
ProtoNetSearch...

Other

GeneWikiGCHFR.
GenomeRNAi2644.
NextBio10430.
PROP30047.
SOURCESearch...

Entry information

Entry nameGFRP_HUMAN
AccessionPrimary (citable) accession number: P30047
Secondary accession number(s): B2R4L6, Q2M1Q2, Q99749
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: January 23, 2007
Last modified: July 9, 2014
This is version 105 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 15

Human chromosome 15: entries, gene names and cross-references to MIM