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Protein

Peroxiredoxin-6

Gene

PRDX6

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in redox regulation of the cell. Can reduce H2O2 and short chain organic, fatty acid, and phospholipid hydroperoxides. May play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury.

Catalytic activityi

2 R'-SH + ROOH = R'-S-S-R' + H2O + ROH.
2 glutathione + H2O2 = glutathione disulfide + 2 H2O.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei32 – 321For phospholipase activity
Active sitei47 – 471Cysteine sulfenic acid (-SOH) intermediate

GO - Molecular functioni

  • cadherin binding involved in cell-cell adhesion Source: BHF-UCL
  • glutathione peroxidase activity Source: UniProtKB-EC
  • hydrolase activity Source: UniProtKB-KW
  • peroxiredoxin activity Source: UniProtKB-EC
  • ubiquitin protein ligase binding Source: ParkinsonsUK-UCL

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Antioxidant, Hydrolase, Oxidoreductase, Peroxidase

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Enzyme and pathway databases

BRENDAi1.11.1.15. 2681.
ReactomeiR-HSA-3299685. Detoxification of Reactive Oxygen Species.
SIGNORiP30041.

Protein family/group databases

PeroxiBasei4426. Hs1CysPrx01.

Names & Taxonomyi

Protein namesi
Recommended name:
Peroxiredoxin-6 (EC:1.11.1.15)
Alternative name(s):
1-Cys peroxiredoxin
Short name:
1-Cys PRX
24 kDa protein
Acidic calcium-independent phospholipase A2 (EC:3.1.1.-)
Short name:
aiPLA2
Antioxidant protein 2
Liver 2D page spot 40
Non-selenium glutathione peroxidase (EC:1.11.1.9)
Short name:
NSGPx
Red blood cells page spot 12
Gene namesi
Name:PRDX6
Synonyms:AOP2, KIAA0106
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:16753. PRDX6.

Subcellular locationi

GO - Cellular componenti

  • cell-cell adherens junction Source: BHF-UCL
  • cytoplasm Source: UniProtKB
  • cytoplasmic, membrane-bounded vesicle Source: UniProtKB-SubCell
  • cytosol Source: UniProtKB
  • extracellular exosome Source: UniProtKB
  • extracellular space Source: UniProtKB
  • lysosome Source: UniProtKB-SubCell
  • membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Lysosome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi32 – 321S → A: Loss of AIPLA2 activity, but no effect on NSGPX activity.
Mutagenesisi47 – 471C → S: Loss of NSGPX activity, but no effect on AIPLA2 activity.

Organism-specific databases

PharmGKBiPA134992760.

Chemistry

DrugBankiDB02325. Isopropyl Alcohol.

Polymorphism and mutation databases

BioMutaiPRDX6.
DMDMi1718024.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedCombined sources4 Publications
Chaini2 – 224223Peroxiredoxin-6PRO_0000135102Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei44 – 441PhosphothreonineCombined sources
Disulfide bondi47 – 47Interchain; in linked formBy similarity
Modified residuei63 – 631N6-acetyllysineCombined sources
Modified residuei89 – 891PhosphotyrosineCombined sources
Modified residuei209 – 2091N6-acetyllysine; alternateCombined sources
Modified residuei209 – 2091N6-succinyllysine; alternateBy similarity

Keywords - PTMi

Acetylation, Disulfide bond, Phosphoprotein

Proteomic databases

EPDiP30041.
PaxDbiP30041.
PeptideAtlasiP30041.
PRIDEiP30041.
TopDownProteomicsiP30041.

2D gel databases

DOSAC-COBS-2DPAGEP30041.
OGPiP30041.
REPRODUCTION-2DPAGEIPI00220301.
P30041.
SWISS-2DPAGEP30041.

PTM databases

iPTMnetiP30041.
PhosphoSiteiP30041.
SwissPalmiP30041.

Expressioni

Gene expression databases

BgeeiENSG00000117592.
CleanExiHS_PRDX6.
ExpressionAtlasiP30041. baseline and differential.
GenevisibleiP30041. HS.

Organism-specific databases

HPAiCAB008663.
HPA006983.

Interactioni

Subunit structurei

Homotetramer. Interacts with GSTP1; mediates PRDX6 glutathionylation and regeneration. May interact with HTR2A (By similarity). Interacts with APEX1 and STH. May interact with FAM168B.By similarity3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
P299913EBI-2255129,EBI-8826747From a different organism.
GSTP1P092112EBI-2255129,EBI-353467
TERF1P542742EBI-2255129,EBI-710997

GO - Molecular functioni

  • cadherin binding involved in cell-cell adhesion Source: BHF-UCL
  • ubiquitin protein ligase binding Source: ParkinsonsUK-UCL

Protein-protein interaction databases

BioGridi114956. 75 interactions.
IntActiP30041. 23 interactions.
MINTiMINT-4999165.
STRINGi9606.ENSP00000342026.

Structurei

Secondary structure

1
224
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi15 – 184Combined sources
Beta strandi21 – 244Combined sources
Helixi25 – 295Combined sources
Beta strandi32 – 409Combined sources
Helixi45 – 6218Combined sources
Turni63 – 653Combined sources
Beta strandi66 – 749Combined sources
Helixi76 – 8914Combined sources
Beta strandi102 – 1043Combined sources
Helixi109 – 1135Combined sources
Beta strandi124 – 1263Combined sources
Beta strandi133 – 1375Combined sources
Beta strandi141 – 1488Combined sources
Helixi157 – 17317Combined sources
Beta strandi175 – 1773Combined sources
Beta strandi187 – 1893Combined sources
Helixi195 – 2017Combined sources
Beta strandi219 – 2213Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1PRXX-ray2.00A/B1-224[»]
ProteinModelPortaliP30041.
SMRiP30041. Positions 5-224.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP30041.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini5 – 169165ThioredoxinPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the AhpC/TSA family. Rehydrin subfamily.Curated
Contains 1 thioredoxin domain.PROSITE-ProRule annotation

Keywords - Domaini

Redox-active center

Phylogenomic databases

eggNOGiKOG0854. Eukaryota.
COG0450. LUCA.
GeneTreeiENSGT00550000074794.
HOGENOMiHOG000022346.
HOVERGENiHBG105234.
InParanoidiP30041.
KOiK11188.
OMAiQLTDNYS.
OrthoDBiEOG091G0IWK.
PhylomeDBiP30041.
TreeFamiTF105183.

Family and domain databases

Gene3Di3.40.30.10. 1 hit.
InterProiIPR000866. AhpC/TSA.
IPR024706. Peroxiredoxin_AhpC-typ.
IPR019479. Peroxiredoxin_C.
IPR012336. Thioredoxin-like_fold.
IPR013766. Thioredoxin_domain.
[Graphical view]
PfamiPF10417. 1-cysPrx_C. 1 hit.
PF00578. AhpC-TSA. 1 hit.
[Graphical view]
PIRSFiPIRSF000239. AHPC. 1 hit.
SUPFAMiSSF52833. SSF52833. 1 hit.
PROSITEiPS51352. THIOREDOXIN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P30041-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPGGLLLGDV APNFEANTTV GRIRFHDFLG DSWGILFSHP RDFTPVCTTE
60 70 80 90 100
LGRAAKLAPE FAKRNVKLIA LSIDSVEDHL AWSKDINAYN CEEPTEKLPF
110 120 130 140 150
PIIDDRNREL AILLGMLDPA EKDEKGMPVT ARVVFVFGPD KKLKLSILYP
160 170 180 190 200
ATTGRNFDEI LRVVISLQLT AEKRVATPVD WKDGDSVMVL PTIPEEEAKK
210 220
LFPKGVFTKE LPSGKKYLRY TPQP
Length:224
Mass (Da):25,035
Last modified:January 23, 2007 - v3
Checksum:i017D955F0FEEDFBC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D14662 mRNA. Translation: BAA03496.1.
DQ230990 Genomic DNA. Translation: ABB02185.1.
AL139142 Genomic DNA. Translation: CAI20936.1.
AK289352 mRNA. Translation: BAF82041.1.
CH471067 Genomic DNA. Translation: EAW90944.1.
BC035857 mRNA. Translation: AAH35857.1.
BC053550 mRNA. Translation: AAH53550.1.
AJ844621 mRNA. Translation: CAH59743.1.
CCDSiCCDS1307.1.
RefSeqiNP_004896.1. NM_004905.2.
UniGeneiHs.120.
Hs.731505.

Genome annotation databases

EnsembliENST00000340385; ENSP00000342026; ENSG00000117592.
GeneIDi9588.
KEGGihsa:9588.

Cross-referencesi

Web resourcesi

NIEHS SNPs

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D14662 mRNA. Translation: BAA03496.1.
DQ230990 Genomic DNA. Translation: ABB02185.1.
AL139142 Genomic DNA. Translation: CAI20936.1.
AK289352 mRNA. Translation: BAF82041.1.
CH471067 Genomic DNA. Translation: EAW90944.1.
BC035857 mRNA. Translation: AAH35857.1.
BC053550 mRNA. Translation: AAH53550.1.
AJ844621 mRNA. Translation: CAH59743.1.
CCDSiCCDS1307.1.
RefSeqiNP_004896.1. NM_004905.2.
UniGeneiHs.120.
Hs.731505.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1PRXX-ray2.00A/B1-224[»]
ProteinModelPortaliP30041.
SMRiP30041. Positions 5-224.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114956. 75 interactions.
IntActiP30041. 23 interactions.
MINTiMINT-4999165.
STRINGi9606.ENSP00000342026.

Chemistry

DrugBankiDB02325. Isopropyl Alcohol.

Protein family/group databases

PeroxiBasei4426. Hs1CysPrx01.

PTM databases

iPTMnetiP30041.
PhosphoSiteiP30041.
SwissPalmiP30041.

Polymorphism and mutation databases

BioMutaiPRDX6.
DMDMi1718024.

2D gel databases

DOSAC-COBS-2DPAGEP30041.
OGPiP30041.
REPRODUCTION-2DPAGEIPI00220301.
P30041.
SWISS-2DPAGEP30041.

Proteomic databases

EPDiP30041.
PaxDbiP30041.
PeptideAtlasiP30041.
PRIDEiP30041.
TopDownProteomicsiP30041.

Protocols and materials databases

DNASUi9588.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000340385; ENSP00000342026; ENSG00000117592.
GeneIDi9588.
KEGGihsa:9588.

Organism-specific databases

CTDi9588.
GeneCardsiPRDX6.
H-InvDBHIX0028696.
HGNCiHGNC:16753. PRDX6.
HPAiCAB008663.
HPA006983.
MIMi602316. gene.
neXtProtiNX_P30041.
PharmGKBiPA134992760.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0854. Eukaryota.
COG0450. LUCA.
GeneTreeiENSGT00550000074794.
HOGENOMiHOG000022346.
HOVERGENiHBG105234.
InParanoidiP30041.
KOiK11188.
OMAiQLTDNYS.
OrthoDBiEOG091G0IWK.
PhylomeDBiP30041.
TreeFamiTF105183.

Enzyme and pathway databases

BRENDAi1.11.1.15. 2681.
ReactomeiR-HSA-3299685. Detoxification of Reactive Oxygen Species.
SIGNORiP30041.

Miscellaneous databases

ChiTaRSiPRDX6. human.
EvolutionaryTraceiP30041.
GeneWikiiPRDX6.
GenomeRNAii9588.
PROiP30041.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000117592.
CleanExiHS_PRDX6.
ExpressionAtlasiP30041. baseline and differential.
GenevisibleiP30041. HS.

Family and domain databases

Gene3Di3.40.30.10. 1 hit.
InterProiIPR000866. AhpC/TSA.
IPR024706. Peroxiredoxin_AhpC-typ.
IPR019479. Peroxiredoxin_C.
IPR012336. Thioredoxin-like_fold.
IPR013766. Thioredoxin_domain.
[Graphical view]
PfamiPF10417. 1-cysPrx_C. 1 hit.
PF00578. AhpC-TSA. 1 hit.
[Graphical view]
PIRSFiPIRSF000239. AHPC. 1 hit.
SUPFAMiSSF52833. SSF52833. 1 hit.
PROSITEiPS51352. THIOREDOXIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPRDX6_HUMAN
AccessioniPrimary (citable) accession number: P30041
Secondary accession number(s): A8JZY7
, P32077, Q5TAH4, Q5ZEZ8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: January 23, 2007
Last modified: September 7, 2016
This is version 180 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

The active site is the redox-active Cys-47 oxidized to Cys-SOH. Unlike 2-Cys peroxiredoxins, PRDX6 conserves only this peroxidatic cysteine. To regenerate and activate the enzyme, the oxidized form is directly reduced to cysteine by glutathione and not thioredoxin.
Irreversibly inactivated by overoxidation of Cys-47 (to Cys-SO3H) upon oxidative stress.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Multifunctional enzyme, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.