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Protein

Tegument protein VP22

Gene

UL49

Organism
Bovine herpesvirus 1.1 (strain Cooper) (BoHV-1) (Infectious bovine rhinotracheitis virus)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Tegument protein that plays different roles during the time course of infection. Participates in both the accumulation of viral mRNAs and viral protein translation at late time of infection. Modulates the RNase activity of the virion host shutoff protein UL41 probably to ensure necessary levels of key cellular mRNAs and proteins. Plays a role in microtubule reorganization that occurs after viral infection by stabilizing microtubule network.By similarity

GO - Molecular functioni

  • histone binding Source: AgBase
  • nucleosome binding Source: AgBase

GO - Biological processi

  • negative regulation of histone acetylation Source: AgBase
  • regulation of nucleosome density Source: AgBase

Names & Taxonomyi

Protein namesi
Recommended name:
Tegument protein VP22
Gene namesi
ORF Names:UL49
OrganismiBovine herpesvirus 1.1 (strain Cooper) (BoHV-1) (Infectious bovine rhinotracheitis virus)
Taxonomic identifieri10323 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeVaricellovirus
Virus hostiBos taurus (Bovine) [TaxID: 9913]

Subcellular locationi

  • Virion tegument By similarity
  • Host cytoplasm By similarity
  • Host nucleus By similarity
  • Host Golgi apparatus By similarity

  • Note: One of the most abundant tegument protein (about 2000 copies per virion). Localizes in the cytoplasm at 8 hours postinfection and in the nucleus at 16 hours postinfection. During virion morphogenesis, this protein probably accumulates at the trans-Golgi where secondary envelopment occurs.By similarity

GO - Cellular componenti

  • host cell cytoplasm Source: AgBase
  • host cell Golgi apparatus Source: UniProtKB-SubCell
  • host cell nucleus Source: UniProtKB
  • viral tegument Source: UniProtKB-SubCell

Keywords - Cellular componenti

Host cytoplasm, Host Golgi apparatus, Host nucleus, Virion, Virion tegument

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi131 – 134PRPR → AAAA: Complete loss of nuclear localization. 1 Publication4
Mutagenesisi204 – 216LDRML…AIRIL → ADRMAKSAAARAA: Complete loss of cytoplasmic localization. Add BLAST13

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001160941 – 258Tegument protein VP22Add BLAST258

Post-translational modificationi

Highly phosphorylated in the host cell. Packaging is selective for underphosphorylated forms.By similarity

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Interacts with gE (via C-terminus); this interaction is necessary for the recruitment of VP22 to the Golgi and its packaging into virions. Interacts with gM (via C-terminus). Interacts with VP16; this interaction allows the formation of a tripartite complex composed of VP16, VP22 and UL41/VHS. Interacts with the capsid-binding protein UL16.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
US3Q76PF33EBI-11420994,EBI-11420970From Bovine alphaherpesvirus 1.

GO - Molecular functioni

  • histone binding Source: AgBase

Protein-protein interaction databases

IntActiP30022. 2 interactors.

Structurei

3D structure databases

SMRiP30022.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi131 – 134Nuclear localization signal1 Publication4
Motifi204 – 216Nuclear export signalAdd BLAST13

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi61 – 91Ala-richAdd BLAST31

Sequence similaritiesi

Family and domain databases

InterProiView protein in InterPro
IPR006908. Herpes_UL49.
PfamiView protein in Pfam
PF04823. Herpes_UL49_2. 1 hit.

Sequencei

Sequence statusi: Complete.

P30022-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARFHRPSED EDDYEYSDLW VRENSLYDYE SGSDDHVYEE LRAATSGPEP
60 70 80 90 100
SGRRASVRAC ASAAAVQPAA RGRDRAAAAG TTVAAPAAAP ARRSSSRASS
110 120 130 140 150
RPPRAAADPP VLRPATRGSS GGAGAVAVGP PRPRAPPGAN AVASGRPLAF
160 170 180 190 200
SAAPKTPKAP WCGPTHAYNR TIFCEAVALV AAEYARQAAA SVWDSDPPKS
210 220 230 240 250
NERLDRMLKS AAIRILVCEG SGLLAAANDI LAARAQRPAA RGSTSGGESR

LRGERARP
Length:258
Mass (Da):26,865
Last modified:October 1, 1996 - v2
Checksum:i78C5567442E8A161
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z54206 Genomic DNA. Translation: CAA90920.1.
U21137 Genomic DNA. Translation: AAA85715.1.
AJ004801 Genomic DNA. Translation: CAA06085.1.
Z11610 Genomic DNA. No translation available.
PIRiS24228.
RefSeqiNP_045310.1. NC_001847.1.

Genome annotation databases

GeneIDi1487397.
KEGGivg:1487397.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z54206 Genomic DNA. Translation: CAA90920.1.
U21137 Genomic DNA. Translation: AAA85715.1.
AJ004801 Genomic DNA. Translation: CAA06085.1.
Z11610 Genomic DNA. No translation available.
PIRiS24228.
RefSeqiNP_045310.1. NC_001847.1.

3D structure databases

SMRiP30022.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP30022. 2 interactors.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1487397.
KEGGivg:1487397.

Family and domain databases

InterProiView protein in InterPro
IPR006908. Herpes_UL49.
PfamiView protein in Pfam
PF04823. Herpes_UL49_2. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiVP22_BHV1C
AccessioniPrimary (citable) accession number: P30022
Secondary accession number(s): Q77CE3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: October 1, 1996
Last modified: February 15, 2017
This is version 62 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.