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Protein

Nicotinate-nucleotide pyrophosphorylase [carboxylating]

Gene

nadC

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the catabolism of quinolinic acid (QA).By similarity

Catalytic activityi

Beta-nicotinate D-ribonucleotide + diphosphate + CO2 = pyridine-2,3-dicarboxylate + 5-phospho-alpha-D-ribose 1-diphosphate.

Pathwayi: NAD(+) biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes nicotinate D-ribonucleotide from quinolinate.
Proteins known to be involved in this subpathway in this organism are:
  1. Nicotinate-nucleotide pyrophosphorylase [carboxylating] (nadC)
This subpathway is part of the pathway NAD(+) biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes nicotinate D-ribonucleotide from quinolinate, the pathway NAD(+) biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei119 – 1191SubstrateBy similarity
Binding sitei176 – 1761Substrate1 Publication
Binding sitei186 – 1861SubstrateBy similarity
Binding sitei215 – 2151SubstrateBy similarity
Binding sitei236 – 2361SubstrateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Pyridine nucleotide biosynthesis

Enzyme and pathway databases

BioCyciSENT99287:GCTI-144-MONOMER.
UniPathwayiUPA00253; UER00331.

Names & Taxonomyi

Protein namesi
Recommended name:
Nicotinate-nucleotide pyrophosphorylase [carboxylating] (EC:2.4.2.19)
Alternative name(s):
Quinolinate phosphoribosyltransferase [decarboxylating]
Short name:
QAPRTase
Gene namesi
Name:nadC
Ordered Locus Names:STM0145
OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Taxonomic identifieri99287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella
Proteomesi
  • UP000001014 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved
Chaini2 – 297296Nicotinate-nucleotide pyrophosphorylase [carboxylating]PRO_0000155949Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21Blocked amino end (Pro)

Proteomic databases

PaxDbiP30012.
PRIDEiP30012.

Interactioni

Subunit structurei

Hexamer formed by 3 homodimers (By similarity). Homodimer.By similarity1 Publication

Protein-protein interaction databases

STRINGi99287.STM0145.

Structurei

Secondary structure

1
297
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi11 – 3424Combined sources
Turni35 – 373Combined sources
Helixi41 – 433Combined sources
Helixi46 – 494Combined sources
Beta strandi59 – 646Combined sources
Helixi71 – 8212Combined sources
Beta strandi85 – 917Combined sources
Beta strandi103 – 1108Combined sources
Helixi111 – 14030Combined sources
Turni141 – 1444Combined sources
Beta strandi148 – 1503Combined sources
Helixi160 – 17011Combined sources
Beta strandi177 – 1815Combined sources
Beta strandi183 – 1853Combined sources
Helixi187 – 1937Combined sources
Helixi196 – 20611Combined sources
Beta strandi212 – 2187Combined sources
Helixi219 – 2279Combined sources
Beta strandi231 – 2377Combined sources
Helixi240 – 2489Combined sources
Beta strandi256 – 2583Combined sources
Helixi264 – 2729Combined sources
Beta strandi276 – 2794Combined sources
Helixi282 – 2854Combined sources
Beta strandi286 – 2883Combined sources
Beta strandi292 – 2954Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1QAPX-ray2.80A/B2-297[»]
ProteinModelPortaliP30012.
SMRiP30012. Positions 9-297.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP30012.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni152 – 1543Substrate binding
Regioni259 – 2613Substrate bindingBy similarity
Regioni280 – 2823Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the NadC/ModD family.Curated

Phylogenomic databases

eggNOGiENOG4105D18. Bacteria.
COG0157. LUCA.
HOGENOMiHOG000224022.
KOiK00767.
OMAiDMIMLKD.
PhylomeDBiP30012.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
3.90.1170.20. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR004393. NadC.
IPR027277. NadC/ModD.
IPR002638. Quinolinate_PRibosylTrfase_C.
IPR022412. Quinolinate_PRibosylTrfase_N.
[Graphical view]
PfamiPF01729. QRPTase_C. 1 hit.
PF02749. QRPTase_N. 1 hit.
[Graphical view]
PIRSFiPIRSF006250. NadC_ModD. 1 hit.
SUPFAMiSSF51690. SSF51690. 1 hit.
TIGRFAMsiTIGR00078. nadC. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P30012-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPPRRYNPDD RRDALLERIN LDIPAAVAQA LREDLGGEVD AGNDITAQLL
60 70 80 90 100
PADTQAHATV ITREDGVFCG KRWVEEVFIQ LAGDDVRLTW HVDDGDAIHA
110 120 130 140 150
NQTVFELNGP ARVLLTGERT ALNFVQTLSG VASEVRRYVG LLAGTQTQLL
160 170 180 190 200
DTRKTLPGLR TALKYAVLCG GGANHRLGLT DAFLIKENHI IASGSVRQAV
210 220 230 240 250
EKAFWLHPDV PVEVEVENLD ELDDALKAGA DIIMLDNFNT DQMREAVKRV
260 270 280 290
NGQARLEVSG NVTAETLREF AETGVDFISV GALTKHVRAL DLSMRFC
Length:297
Mass (Da):32,560
Last modified:January 23, 2007 - v3
Checksum:i67F226C4D7C478ED
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L07292 Genomic DNA. Translation: AAA03225.1.
AE006468 Genomic DNA. Translation: AAL19109.1.
RefSeqiNP_459150.1. NC_003197.1.
WP_001135135.1. NC_003197.1.

Genome annotation databases

EnsemblBacteriaiAAL19109; AAL19109; STM0145.
GeneIDi1251663.
KEGGistm:STM0145.
PATRICi32378569. VBISalEnt20916_0154.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L07292 Genomic DNA. Translation: AAA03225.1.
AE006468 Genomic DNA. Translation: AAL19109.1.
RefSeqiNP_459150.1. NC_003197.1.
WP_001135135.1. NC_003197.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1QAPX-ray2.80A/B2-297[»]
ProteinModelPortaliP30012.
SMRiP30012. Positions 9-297.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi99287.STM0145.

Proteomic databases

PaxDbiP30012.
PRIDEiP30012.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL19109; AAL19109; STM0145.
GeneIDi1251663.
KEGGistm:STM0145.
PATRICi32378569. VBISalEnt20916_0154.

Phylogenomic databases

eggNOGiENOG4105D18. Bacteria.
COG0157. LUCA.
HOGENOMiHOG000224022.
KOiK00767.
OMAiDMIMLKD.
PhylomeDBiP30012.

Enzyme and pathway databases

UniPathwayiUPA00253; UER00331.
BioCyciSENT99287:GCTI-144-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP30012.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
3.90.1170.20. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR004393. NadC.
IPR027277. NadC/ModD.
IPR002638. Quinolinate_PRibosylTrfase_C.
IPR022412. Quinolinate_PRibosylTrfase_N.
[Graphical view]
PfamiPF01729. QRPTase_C. 1 hit.
PF02749. QRPTase_N. 1 hit.
[Graphical view]
PIRSFiPIRSF006250. NadC_ModD. 1 hit.
SUPFAMiSSF51690. SSF51690. 1 hit.
TIGRFAMsiTIGR00078. nadC. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNADC_SALTY
AccessioniPrimary (citable) accession number: P30012
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: January 23, 2007
Last modified: September 7, 2016
This is version 116 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.