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Protein

Nicotinate-nucleotide pyrophosphorylase [carboxylating]

Gene

nadC

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the catabolism of quinolinic acid (QA).By similarity

Catalytic activityi

Beta-nicotinate D-ribonucleotide + diphosphate + CO2 = pyridine-2,3-dicarboxylate + 5-phospho-alpha-D-ribose 1-diphosphate.

Pathwayi: NAD(+) biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes nicotinate D-ribonucleotide from quinolinate.
Proteins known to be involved in this subpathway in this organism are:
  1. Nicotinate-nucleotide pyrophosphorylase [carboxylating] (nadC)
This subpathway is part of the pathway NAD(+) biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes nicotinate D-ribonucleotide from quinolinate, the pathway NAD(+) biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei119SubstrateBy similarity1
Binding sitei176Substrate1 Publication1
Binding sitei186SubstrateBy similarity1
Binding sitei215SubstrateBy similarity1
Binding sitei236SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Pyridine nucleotide biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00253; UER00331.

Names & Taxonomyi

Protein namesi
Recommended name:
Nicotinate-nucleotide pyrophosphorylase [carboxylating] (EC:2.4.2.19)
Alternative name(s):
Quinolinate phosphoribosyltransferase [decarboxylating]
Short name:
QAPRTase
Gene namesi
Name:nadC
Ordered Locus Names:STM0145
OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Taxonomic identifieri99287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeSalmonella
Proteomesi
  • UP000001014 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved
ChainiPRO_00001559492 – 297Nicotinate-nucleotide pyrophosphorylase [carboxylating]Add BLAST296

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2Blocked amino end (Pro)1

Proteomic databases

PaxDbiP30012.
PRIDEiP30012.

Interactioni

Subunit structurei

Hexamer formed by 3 homodimers (By similarity). Homodimer.By similarity1 Publication

Protein-protein interaction databases

STRINGi99287.STM0145.

Structurei

Secondary structure

1297
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi11 – 34Combined sources24
Turni35 – 37Combined sources3
Helixi41 – 43Combined sources3
Helixi46 – 49Combined sources4
Beta strandi59 – 64Combined sources6
Helixi71 – 82Combined sources12
Beta strandi85 – 91Combined sources7
Beta strandi103 – 110Combined sources8
Helixi111 – 140Combined sources30
Turni141 – 144Combined sources4
Beta strandi148 – 150Combined sources3
Helixi160 – 170Combined sources11
Beta strandi177 – 181Combined sources5
Beta strandi183 – 185Combined sources3
Helixi187 – 193Combined sources7
Helixi196 – 206Combined sources11
Beta strandi212 – 218Combined sources7
Helixi219 – 227Combined sources9
Beta strandi231 – 237Combined sources7
Helixi240 – 248Combined sources9
Beta strandi256 – 258Combined sources3
Helixi264 – 272Combined sources9
Beta strandi276 – 279Combined sources4
Helixi282 – 285Combined sources4
Beta strandi286 – 288Combined sources3
Beta strandi292 – 295Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1QAPX-ray2.80A/B2-297[»]
ProteinModelPortaliP30012.
SMRiP30012.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP30012.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni152 – 154Substrate binding3
Regioni259 – 261Substrate bindingBy similarity3
Regioni280 – 282Substrate bindingBy similarity3

Sequence similaritiesi

Belongs to the NadC/ModD family.Curated

Phylogenomic databases

eggNOGiENOG4105D18. Bacteria.
COG0157. LUCA.
HOGENOMiHOG000224022.
KOiK00767.
OMAiDMIMLKD.
PhylomeDBiP30012.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
3.90.1170.20. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR004393. NadC.
IPR027277. NadC/ModD.
IPR002638. Quinolinate_PRibosylTrfase_C.
IPR022412. Quinolinate_PRibosylTrfase_N.
[Graphical view]
PfamiPF01729. QRPTase_C. 1 hit.
PF02749. QRPTase_N. 1 hit.
[Graphical view]
PIRSFiPIRSF006250. NadC_ModD. 1 hit.
SUPFAMiSSF51690. SSF51690. 1 hit.
TIGRFAMsiTIGR00078. nadC. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P30012-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPPRRYNPDD RRDALLERIN LDIPAAVAQA LREDLGGEVD AGNDITAQLL
60 70 80 90 100
PADTQAHATV ITREDGVFCG KRWVEEVFIQ LAGDDVRLTW HVDDGDAIHA
110 120 130 140 150
NQTVFELNGP ARVLLTGERT ALNFVQTLSG VASEVRRYVG LLAGTQTQLL
160 170 180 190 200
DTRKTLPGLR TALKYAVLCG GGANHRLGLT DAFLIKENHI IASGSVRQAV
210 220 230 240 250
EKAFWLHPDV PVEVEVENLD ELDDALKAGA DIIMLDNFNT DQMREAVKRV
260 270 280 290
NGQARLEVSG NVTAETLREF AETGVDFISV GALTKHVRAL DLSMRFC
Length:297
Mass (Da):32,560
Last modified:January 23, 2007 - v3
Checksum:i67F226C4D7C478ED
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L07292 Genomic DNA. Translation: AAA03225.1.
AE006468 Genomic DNA. Translation: AAL19109.1.
RefSeqiNP_459150.1. NC_003197.1.
WP_001135135.1. NC_003197.1.

Genome annotation databases

EnsemblBacteriaiAAL19109; AAL19109; STM0145.
GeneIDi1251663.
KEGGistm:STM0145.
PATRICi32378569. VBISalEnt20916_0154.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L07292 Genomic DNA. Translation: AAA03225.1.
AE006468 Genomic DNA. Translation: AAL19109.1.
RefSeqiNP_459150.1. NC_003197.1.
WP_001135135.1. NC_003197.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1QAPX-ray2.80A/B2-297[»]
ProteinModelPortaliP30012.
SMRiP30012.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi99287.STM0145.

Proteomic databases

PaxDbiP30012.
PRIDEiP30012.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL19109; AAL19109; STM0145.
GeneIDi1251663.
KEGGistm:STM0145.
PATRICi32378569. VBISalEnt20916_0154.

Phylogenomic databases

eggNOGiENOG4105D18. Bacteria.
COG0157. LUCA.
HOGENOMiHOG000224022.
KOiK00767.
OMAiDMIMLKD.
PhylomeDBiP30012.

Enzyme and pathway databases

UniPathwayiUPA00253; UER00331.

Miscellaneous databases

EvolutionaryTraceiP30012.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
3.90.1170.20. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR004393. NadC.
IPR027277. NadC/ModD.
IPR002638. Quinolinate_PRibosylTrfase_C.
IPR022412. Quinolinate_PRibosylTrfase_N.
[Graphical view]
PfamiPF01729. QRPTase_C. 1 hit.
PF02749. QRPTase_N. 1 hit.
[Graphical view]
PIRSFiPIRSF006250. NadC_ModD. 1 hit.
SUPFAMiSSF51690. SSF51690. 1 hit.
TIGRFAMsiTIGR00078. nadC. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNADC_SALTY
AccessioniPrimary (citable) accession number: P30012
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 118 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.