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Protein

Nicotinate-nucleotide pyrophosphorylase [carboxylating]

Gene

nadC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Involved in the catabolism of quinolinic acid (QA).By similarity

Catalytic activityi

Beta-nicotinate D-ribonucleotide + diphosphate + CO2 = pyridine-2,3-dicarboxylate + 5-phospho-alpha-D-ribose 1-diphosphate.

Pathwayi: NAD(+) biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes nicotinate D-ribonucleotide from quinolinate.
Proteins known to be involved in this subpathway in this organism are:
  1. Nicotinate-nucleotide pyrophosphorylase [carboxylating] (nadC)
This subpathway is part of the pathway NAD(+) biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes nicotinate D-ribonucleotide from quinolinate, the pathway NAD(+) biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei119 – 1191SubstrateBy similarity
Binding sitei176 – 1761SubstrateBy similarity
Binding sitei186 – 1861SubstrateBy similarity
Binding sitei215 – 2151SubstrateBy similarity
Binding sitei236 – 2361SubstrateBy similarity

GO - Molecular functioni

  • nicotinate-nucleotide diphosphorylase (carboxylating) activity Source: EcoCyc

GO - Biological processi

  • 'de novo' NAD biosynthetic process from aspartate Source: EcoCyc
  • NAD biosynthetic process Source: EcoliWiki
  • quinolinate catabolic process Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Pyridine nucleotide biosynthesis

Enzyme and pathway databases

BioCyciEcoCyc:QUINOPRIBOTRANS-MONOMER.
ECOL316407:JW0105-MONOMER.
MetaCyc:QUINOPRIBOTRANS-MONOMER.
BRENDAi2.4.2.19. 2026.
UniPathwayiUPA00253; UER00331.

Names & Taxonomyi

Protein namesi
Recommended name:
Nicotinate-nucleotide pyrophosphorylase [carboxylating] (EC:2.4.2.19)
Alternative name(s):
Quinolinate phosphoribosyltransferase [decarboxylating]
Short name:
QAPRTase
Gene namesi
Name:nadC
Ordered Locus Names:b0109, JW0105
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11546. nadC.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: EcoliWiki
  • cytosol Source: EcoCyc
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 297296Nicotinate-nucleotide pyrophosphorylase [carboxylating]PRO_0000155942Add
BLAST

Proteomic databases

EPDiP30011.
PaxDbiP30011.
PRIDEiP30011.

Interactioni

Subunit structurei

Homodimer Hexamer formed by 3 homodimers (By similarity). Homodimer.By similarity

Protein-protein interaction databases

BioGridi4263544. 5 interactions.
DIPiDIP-10293N.
IntActiP30011. 3 interactions.
MINTiMINT-1227037.
STRINGi511145.b0109.

Structurei

3D structure databases

ProteinModelPortaliP30011.
SMRiP30011. Positions 9-296.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni152 – 1543Substrate bindingBy similarity
Regioni259 – 2613Substrate bindingBy similarity
Regioni280 – 2823Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the NadC/ModD family.Curated

Phylogenomic databases

eggNOGiENOG4105D18. Bacteria.
COG0157. LUCA.
HOGENOMiHOG000224022.
InParanoidiP30011.
KOiK00767.
OMAiDMIMLKD.
PhylomeDBiP30011.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
3.90.1170.20. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR004393. NadC.
IPR027277. NadC/ModD.
IPR002638. Quinolinate_PRibosylTrfase_C.
IPR022412. Quinolinate_PRibosylTrfase_N.
[Graphical view]
PfamiPF01729. QRPTase_C. 1 hit.
PF02749. QRPTase_N. 1 hit.
[Graphical view]
PIRSFiPIRSF006250. NadC_ModD. 1 hit.
SUPFAMiSSF51690. SSF51690. 1 hit.
TIGRFAMsiTIGR00078. nadC. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P30011-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPPRRYNPDT RRDELLERIN LDIPGAVAQA LREDLGGTVD ANNDITAKLL
60 70 80 90 100
PENSRSHATV ITRENGVFCG KRWVEEVFIQ LAGDDVTIIW HVDDGDVINA
110 120 130 140 150
NQSLFELEGP SRVLLTGERT ALNFVQTLSG VASKVRHYVE LLEGTNTQLL
160 170 180 190 200
DTRKTLPGLR SALKYAVLCG GGANHRLGLS DAFLIKENHI IASGSVRQAV
210 220 230 240 250
EKASWLHPDA PVEVEVENLE ELDEALKAGA DIIMLDNFET EQMREAVKRT
260 270 280 290
NGKALLEVSG NVTDKTLREF AETGVDFISV GALTKHVQAL DLSMRFR
Length:297
Mass (Da):32,762
Last modified:January 23, 2007 - v7
Checksum:i0B5DA69D9B67EE39
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti119 – 1191R → P in AAB00467 (PubMed:8561507).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L28105 Genomic DNA. Translation: AAC36922.1.
L20833 Genomic DNA. Translation: AAB00467.1.
U00096 Genomic DNA. Translation: AAC73220.1.
AP009048 Genomic DNA. Translation: BAE76041.1.
X15237 Genomic DNA. No translation available.
PIRiE64733.
RefSeqiNP_414651.1. NC_000913.3.
WP_001135174.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73220; AAC73220; b0109.
BAE76041; BAE76041; BAE76041.
GeneIDi948869.
KEGGiecj:JW0105.
eco:b0109.
PATRICi32115319. VBIEscCol129921_0111.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L28105 Genomic DNA. Translation: AAC36922.1.
L20833 Genomic DNA. Translation: AAB00467.1.
U00096 Genomic DNA. Translation: AAC73220.1.
AP009048 Genomic DNA. Translation: BAE76041.1.
X15237 Genomic DNA. No translation available.
PIRiE64733.
RefSeqiNP_414651.1. NC_000913.3.
WP_001135174.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP30011.
SMRiP30011. Positions 9-296.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263544. 5 interactions.
DIPiDIP-10293N.
IntActiP30011. 3 interactions.
MINTiMINT-1227037.
STRINGi511145.b0109.

Proteomic databases

EPDiP30011.
PaxDbiP30011.
PRIDEiP30011.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73220; AAC73220; b0109.
BAE76041; BAE76041; BAE76041.
GeneIDi948869.
KEGGiecj:JW0105.
eco:b0109.
PATRICi32115319. VBIEscCol129921_0111.

Organism-specific databases

EchoBASEiEB1508.
EcoGeneiEG11546. nadC.

Phylogenomic databases

eggNOGiENOG4105D18. Bacteria.
COG0157. LUCA.
HOGENOMiHOG000224022.
InParanoidiP30011.
KOiK00767.
OMAiDMIMLKD.
PhylomeDBiP30011.

Enzyme and pathway databases

UniPathwayiUPA00253; UER00331.
BioCyciEcoCyc:QUINOPRIBOTRANS-MONOMER.
ECOL316407:JW0105-MONOMER.
MetaCyc:QUINOPRIBOTRANS-MONOMER.
BRENDAi2.4.2.19. 2026.

Miscellaneous databases

PROiP30011.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
3.90.1170.20. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR004393. NadC.
IPR027277. NadC/ModD.
IPR002638. Quinolinate_PRibosylTrfase_C.
IPR022412. Quinolinate_PRibosylTrfase_N.
[Graphical view]
PfamiPF01729. QRPTase_C. 1 hit.
PF02749. QRPTase_N. 1 hit.
[Graphical view]
PIRSFiPIRSF006250. NadC_ModD. 1 hit.
SUPFAMiSSF51690. SSF51690. 1 hit.
TIGRFAMsiTIGR00078. nadC. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNADC_ECOLI
AccessioniPrimary (citable) accession number: P30011
Secondary accession number(s): Q2MCG5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: January 23, 2007
Last modified: September 7, 2016
This is version 143 of the entry and version 7 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.