P30008 (SIR_SYNE7) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sulfite reductase [ferredoxin]
EC=1.8.7.1

Gene names

Name:	sir
Ordered Locus Names:	Synpcc7942_0019

Organism

Synechococcus elongatus (strain PCC 7942) (Anacystis nidulans R2) [Complete proteome] [HAMAP]

Taxonomic identifier

1140 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechococcus

Protein attributes

Sequence length	624 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Hydrogen sulfide + 6 oxidized ferredoxin + 3 H2O = sulfite + 6 reduced ferredoxin + 6 H+.
Cofactor	Binds 1 siroheme per subunit. Binds 1 4Fe-4S cluster per subunit.
Subunit structure	Monomer.
Sequence similarities	Belongs to the nitrite and sulfite reductase 4Fe-4S domain family.

Ontologies

Keywords
Ligand	4Fe-4S Heme Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
PTM	Thioether bond
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW heme binding Inferred from electronic annotation. Source: InterPro sulfite reductase (ferredoxin) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 624	624	Sulfite reductase [ferredoxin]		PRO_0000199953
Sites
	Metal binding	446	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	452	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	491	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	495	1	Iron (siroheme axial ligand) By similarity
	Metal binding	495	1	Iron-sulfur (4Fe-4S) By similarity
Amino acid modifications
	Cross-link	52 ↔ 137		3'-(S-cysteinyl)-tyrosine (Tyr-Cys) By similarity
Experimental info
	Sequence conflict	587	1	R → G in CAA77809. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P30008 [UniParc]. Last modified April 18, 2006. Version 2. Checksum: E5AABAFD6BBF4539	FASTA	624	70,131
	10 20 30 40 50 60 MSPTAAPQKL SKVEDLKARS QYLLEPILSQ LQEESTHFNE DGIQILKFHG SYQQDNRDNR 70 80 90 100 110 120 VKGQEKDFQF MLRLRSPGGY IPPQLYLTLD QLADDYGNGT LRATTRQAFQ LHGILKRDLK 130 140 150 160 170 180 TVIRRIVENL GSTISACGDV NRNVMAPPAP FRDRPEYEWA RTYANNIADL LTPESGAYYE 190 200 210 220 230 240 LWLDGEKVLS GEPDPAVLAA RRNPKGRVAD SVEPLYSDRY LPRKFKIAVT VPGDNSIDLF 250 260 270 280 290 300 TQDIGLVVIG NDRGELEGFN VYVGGGMGRT HNKEETFARL ADPLGFVPAA DIYAAVQAIV 310 320 330 340 350 360 ATQRDYGDRS NRRHARMKYL IHDWGIAKFK EAVESVFGKA IAPVRELPPF RYRDYLGWHE 370 380 390 400 410 420 QGDGKWFLGL PITSGRIKDD GNWQLRSALR EIVSRWQLPL LLTGSQDVLI YDVQPGDRAA 430 440 450 460 470 480 IDKLLRDRGV HTVEAIDSLQ RYAMACPALP TCGLAITESE RALPGLLVRI RRLLEEQGLP 490 500 510 520 530 540 DEHFVVRMTG CPNGCARPYM AELAFVGSAP NTYQLWLGGS PDQTRLARPF IDRLADGDVE 550 560 570 580 590 600 TQLRPLFVFF KQSRQAGESF GDFCDRVGFD ALRQFSESYQ HEAAKPRYRV GLRADVHGRL 610 620 KAEADKRGVS LTDLACEAIA AYLR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The ferredoxin:sulphite reductase gene from Synechococcus PCC7942." Gisselmann G., Klausmeier P., Schwenn J.D. Biochim. Biophys. Acta 1144:102-106(1993) [PubMed: 8347657] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete sequence of chromosome 1 of Synechococcus elongatus PCC 7942." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Schmutz J., Larimer F., Land M., Kyrpides N., Lykidis A., Richardson P. Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PCC 7942.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z11755 Genomic DNA. Translation: CAA77809.1. CP000100 Genomic DNA. Translation: ABB56051.1.
RefSeq	YP_399038.1. NC_007604.1.
3D structure databases
ProteinModelPortal	P30008.
ModBase	Search...
Protein-protein interaction databases
STRING	P30008.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3774239.
GenomeReviews	Gene locus Synpcc7942_0019 in contig CP000100_GR.
KEGG	syf:Synpcc7942_0019.
PATRIC	23785063. VBISynElo51371_0082.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0155.
HOGENOM	HBG626671.
PhylomeDB	P30008.
ProtClustDB	PRK13504.
Enzyme and pathway databases
BioCyc	SYNEL:SYNPCC7942_0019-MONOMER.
Family and domain databases
InterPro	IPR005117. NiRdtase/SiRdtase_haem-b_fer. IPR006067. NO2/SO3_Rdtase_4Fe4S_dom. IPR006066. NO2/SO3_Rdtase_FeS/sirohaem_BS. IPR011787. SO3_Rdtase_ferredoxin-dep. [Graphical view]
Gene3D	G3DSA:3.90.480.10. G3DSA:3.90.480.10. 2 hits.
KO	K00392.
Pfam	PF01077. NIR_SIR. 2 hits. PF03460. NIR_SIR_ferr. 2 hits. [Graphical view]
PRINTS	PR00397. SIROHAEM.
SUPFAM	SSF55124. NiR_SiRalpha_1/3. 2 hits.
TIGRFAMs	TIGR02042. Sir. 1 hit.
PROSITE	PS00365. NIR_SIR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SIR_SYNE7

Accession

Primary (citable) accession number: P30008
Secondary accession number(s): Q31SB8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 18, 2006
Last modified:	January 25, 2012
This is version 81 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program