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P30002

- DEN_HHV6G

UniProt

P30002 - DEN_HHV6G

Protein

Deneddylase

Gene

U31

Organism
Human herpesvirus 6A (strain GS) (HHV-6 variant A) (Human B lymphotropic virus)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 48 (01 Oct 2014)
      Sequence version 1 (01 Apr 1993)
      Previous versions | rss
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    Functioni

    Deneddylase that deregulates the host cell cycle S phase to create a favorable environment allowing efficient viral genome replication. Interacts with and deneddylates host cullins including CUL1 and CUL4A, thereby reducing their E3 ubiquitin ligase activity. Inhibition of cullins leads to the stabilization of cullin-RING ligase substrates such as host CDN1A/p21, CDKN1B/p27kip and CDC25A, preventing S phase progression. Additionally, acts as a deubiquitinase and cleaves both 'Lys-48' and 'Lys-63'-linked ubiquitin chains By similarity.By similarity

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei23 – 231PROSITE-ProRule annotation
    Active sitei156 – 1561PROSITE-ProRule annotation
    Active sitei158 – 1581PROSITE-ProRule annotation

    GO - Molecular functioni

    1. cysteine-type peptidase activity Source: UniProtKB-KW

    GO - Biological processi

    1. modulation by virus of host protein ubiquitination Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Host-virus interaction, Modulation of host ubiquitin pathway by viral deubiquitinase, Modulation of host ubiquitin pathway by virus, Ubl conjugation pathway

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Deneddylase (EC:3.4.19.12, EC:3.4.22.-)
    Alternative name(s):
    Ubiquitin thioesterase
    Gene namesi
    Name:U31
    OrganismiHuman herpesvirus 6A (strain GS) (HHV-6 variant A) (Human B lymphotropic virus)
    Taxonomic identifieri10369 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeBetaherpesvirinaeRoseolovirus
    Virus hostiHomo sapiens (Human) [TaxID: 9606]

    Subcellular locationi

    Virion tegument. Host cytoplasm. Host nucleus By similarity
    Note: Tightly associated with the capsid.By similarity

    GO - Cellular componenti

    1. host cell cytoplasm Source: UniProtKB-SubCell
    2. host cell nucleus Source: UniProtKB-SubCell
    3. viral tegument Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Host cytoplasm, Host nucleus, Virion, Virion tegument

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 20772077DeneddylasePRO_0000116040Add
    BLAST

    Interactioni

    Subunit structurei

    Interacts with host CUL1 and CUL4A; these interactions inhibit the E3 ligase activity of cullins.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP30002.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini3 – 221219Peptidase C76PROSITE-ProRule annotationAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili496 – 52429Sequence AnalysisAdd
    BLAST
    Coiled coili1114 – 115441Sequence AnalysisAdd
    BLAST
    Coiled coili1185 – 121430Sequence AnalysisAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi280 – 2834Poly-Lys
    Compositional biasi375 – 3784Poly-Gln

    Sequence similaritiesi

    Belongs to the herpesviridae deneddylase family.Curated
    Contains 1 peptidase C76 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil, Repeat

    Family and domain databases

    InterProiIPR006928. Pept_C76_UL36-USP.
    [Graphical view]
    PfamiPF04843. Herpes_teg_N. 1 hit.
    [Graphical view]
    PROSITEiPS51521. HTUSP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P30002-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKIITSSTNQ NDSKYGPRAG KQCMSNSFSF LHTVYLNGIN NSLNAGTIDA     50
    IMEEGYHLDT AGTLALMLNN SDSQDYRLPT EIPKRIHSRY GVTQHELSRP 100
    FNGTLDTQKI DNEVYLGLID LILYGKSKNG PTFAVITIGV LSRALFFLNN 150
    TLYLFDSHPT EREATAAIYI CQNIEEVYEL LTTHGTEGFY YDASLIFFIE 200
    TSNLSLSSHD AELLILKTYK DPDIAIALDK FSSTEIHEIK KTDDIGSQQD 250
    LVADKTTDLE HAPHKRKKNS HSLELELNDK KKKDTASLTY YATEVDLIPS 300
    FYELRSQFQS LFHDLKSFPI MKSNFNWTIY LQDSPINPNQ PFATPFLWNR 350
    VFHLLCQIID VFVGVGSTND DSSKQQQQTI FINYLLPFKD FSEVFNEALA 400
    ACQENNLDII FIYNNYLCKT TTFRTLERIL LSKFLAIVDN DHKKHYEWVK 450
    SWTTQMFQGM PKKLDDIENY LKAYVDHNPV KHFHEFICLN KAEKYKVAVL 500
    LNEKRKEIQE AIEREKNSFA QLSNFIDKLG ETPALPIESE NVHKVHTSDI 550
    TEGIVPRFIT ESIELPNIST LNNTQQISLD KQLNEKLTNT IHTLTNKFTK 600
    IVQDNYNNIA AGFMPVTELN CLFAYLVNLY FNIEVLKHSG LNINTVLLQE 650
    VEKLYDNTQF LRFGTSHFNI NNLSNFTLSI RKMFVDFYNS QKPSDRASEI 700
    LAAIESILAD PSKNKTVVNI EMIKSQLEEL GKMEISTTEN KQTAAITKQI 750
    LGDQELTPIY DFLHHLSAYN LPNTTTVKNL HLHFILEQRP DIAMTLHDKI 800
    QSILDIYVDD MLNDITVPEQ TFSTVLFLVD LFPNSTEKTA LFESVLTLRQ 850
    LAKKCANLKT LDEFDDLAQF ITTNSEQLQN MMRQHFGKKI PTLMGHIKFL 900
    YSQKIITTEE KNWIQRAKTV VITSPEELTA FLATAPTKHA LQTCKPDLDK 950
    ALQRHMEEQM KQTAENDKKH ILTIRSTLEK RLNDILLILK DGQFSSLETM 1000
    HLNLLETFLK QLQDNNVIIH FTHALLPVLK DIETTISKII SDVIEKILIK 1050
    TPLNPEQMSK EEQKYTPLLS FLSKFKKTTF CTEDVKTEID QMQKSITFLK 1100
    KIATSANKHT RLSHSIYGQE LNLYEERITE LRKETNKMKE QLSKEYALAE 1150
    KKILLSSQDA KTDKIYLVLN THTLKEIKNT QFRETAFAKA LTVEVNNKEN 1200
    QLQELLNHFN AHLKAKMDQN HITKLSFDTK WTAFVSDSRL YIPDFINIKL 1250
    QDFISDPFKV ISQLMNKATN EMPYIQAEIT LKWLTKLIHD INKFCLSAIS 1300
    EFGKEAIPFN YAALRDLEYQ INTKYVEIEN KVICNETVEN TKNIPKLTKL 1350
    LKQLVPKRVA GGQEQYQTLM NKILSSETSM QQTYEKEQLK KEYFEIVNNV 1400
    ASFKLAFNFP QQLQNVERLI EKFKSLPKSQ PFEKFPQEND LLSDSLNTEN 1450
    YINGLRALLN FITAAQNYIQ NTLLKQWAVF QQQNFIPIDY SVANVKPISD 1500
    LYARLRIERE RQVFYQVNSV FGTHLIVDDT GVPLQFHNIF NNAIVKFFSL 1550
    NYKQIHVPED TPRLVSSQYK LLSVCKSFIM ILQQFWENII TLDLGPYLRD 1600
    GTQNFKRELI PIVNLKLFIY CITQAWTASE DSTVSTAFEL PIKQFTLLIL 1650
    CSHPEYLYGC LSHSTDLVIN SLAKSIDKNS LYNTFVVSHN PPEKPMHLMR 1700
    NICIDTQLWQ PAKLMKDTFQ QTFFTQLCPK NEKFFIYLTA FLILPYKFMN 1750
    YIWIQYKPAI FTQRSYQNLI KDLCSEYVHQ NKITTSSVTP HEPDTIKSGE 1800
    RITSKITVHK AQNTPTLTRL QAQEYVFDYI LYSFLTGYEM TFAMYIDTIE 1850
    KTYLLCMRHL ENVLHDKDFQ SVLRARTFDI DYILKQSWTK NIVEHSLFSV 1900
    QLDKIVSYLN HTNRATPNIP LILFNYDNEV VNVYLPPMST NPKKVAFYIK 1950
    NPFHFPVQEY EATNLISFHL YPKTTDILNQ LPPNNTESTR PGKQTSETLT 2000
    TKNLSEPKFK KPAVTGLMPK SQSIILSTDT NVPETSPDVK ANTASAAIKD 2050
    VTLAREKINE FSESINTTIS KLKSMYL 2077
    Length:2,077
    Mass (Da):239,910
    Last modified:April 1, 1993 - v1
    Checksum:i28E297FDC2FAD2AE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S57540 Genomic DNA. Translation: AAB19786.1.
    PIRiH40511. WZBEZ4.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S57540 Genomic DNA. Translation: AAB19786.1 .
    PIRi H40511. WZBEZ4.

    3D structure databases

    ProteinModelPortali P30002.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR006928. Pept_C76_UL36-USP.
    [Graphical view ]
    Pfami PF04843. Herpes_teg_N. 1 hit.
    [Graphical view ]
    PROSITEi PS51521. HTUSP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification of the human herpesvirus 6 glycoprotein H and putative large tegument protein genes."
      Josephs S.F., Ablashi D.V., Salahuddin S.Z., Jagodzinski L.L., Wong-Staal F., Gallo R.C.
      J. Virol. 65:5597-5604(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

    Entry informationi

    Entry nameiDEN_HHV6G
    AccessioniPrimary (citable) accession number: P30002
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1993
    Last sequence update: April 1, 1993
    Last modified: October 1, 2014
    This is version 48 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3