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P29996

- LHDAG_HDVD3

UniProt

P29996 - LHDAG_HDVD3

Protein

Large delta antigen

Gene
N/A
Organism
Hepatitis delta virus genotype I (isolate D380) (HDV)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 64 (01 Oct 2014)
      Sequence version 2 (01 Mar 2004)
      Previous versions | rss
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    Functioni

    Following virus entry into host cell, provides nuclear import of HDV RNPs thanks to its nuclear localization signal. Needs co-infection with hepatitis B virus to provide surface proteins, otherwise there is no packaging or budding. Packages the HDV ribonucleoprotein in hepatitis B virus empty particles. Interacts with both HDV genomic RNA and cytoplasmic tail of HBsAg. May inhibit viral RNA replication.2 Publications

    GO - Molecular functioni

    1. RNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. viral entry into host cell Source: UniProtKB-KW
    2. viral penetration into host nucleus Source: UniProtKB-KW

    Keywords - Biological processi

    Viral penetration into host nucleus, Virus entry into host cell

    Keywords - Ligandi

    RNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Large delta antigen
    Short name:
    L-HDAg
    Alternative name(s):
    p27
    OrganismiHepatitis delta virus genotype I (isolate D380) (HDV)
    Taxonomic identifieri31762 [NCBI]
    Taxonomic lineageiVirusesDeltavirus
    Virus hostiHomo sapiens (Human) [TaxID: 9606]
    ProteomesiUP000006675: Genome

    Subcellular locationi

    Virion 1 Publication. Host nucleushost nucleolus 1 Publication
    Note: isoprenylated in the cytoplasm, and translocates in the nucleus possibly after phosphorylation. Translocates after to nuclear speckle, then to the ER membrane where interaction with Hepatitis B virus antigene takes place.

    GO - Cellular componenti

    1. host cell nucleolus Source: UniProtKB-SubCell
    2. intracellular Source: GOC
    3. virion Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Host nucleus, Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 211211Large delta antigenPRO_0000038130Add
    BLAST
    Propeptidei212 – 2143Removed in mature formCuratedPRO_0000396787

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21Phosphoserine; by host1 Publication
    Modified residuei13 – 131Omega-N-methylated arginine; by host
    Modified residuei72 – 721N6-acetyllysine; by host
    Modified residuei123 – 1231Phosphoserine; by host1 Publication
    Modified residuei177 – 1771Phosphoserine; by host1 Publication
    Modified residuei211 – 2111Cysteine methyl ester; by hostCurated
    Lipidationi211 – 2111S-farnesyl cysteine; by host2 Publications

    Post-translational modificationi

    Prenylated by host farnesyl-transferase in the cytoplasm prior to nucleus translocation.2 Publications
    Phosphorylated at serines by host CK2 and other kinases. phosphorylation does not seem to be important for its function.1 Publication

    Keywords - PTMi

    Acetylation, Lipoprotein, Methylation, Phosphoprotein, Prenylation

    Interactioni

    Subunit structurei

    Homodimer. Homooctamer. Interacts with HBV HBsAg. May interact with clathrin to induce virion budding.2 Publications

    Protein-protein interaction databases

    MINTiMINT-196884.

    Structurei

    3D structure databases

    ProteinModelPortaliP29996.
    SMRiP29996. Positions 12-60.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni12 – 6049DimerizationSequence AnalysisAdd
    BLAST
    Regioni97 – 10711RNA-bindingSequence AnalysisAdd
    BLAST
    Regioni136 – 14611RNA-bindingSequence AnalysisAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi66 – 7510Nuclear localization signalBy similarity

    Sequence similaritiesi

    Belongs to the hepatitis delta antigen family.Curated

    Family and domain databases

    Gene3Di4.10.220.40. 1 hit.
    InterProiIPR027403. Delta_antigen_N.
    IPR002506. HDV_ag.
    [Graphical view]
    PfamiPF01517. HDV_ag. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P29996-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSRPEGRKNR GGREEVLEQW VSGRKKLEEL ERDLRKVKKK IKKLEDEHPW    50
    LGNIKGILGK KDKDGEGAPP AKRARTDQME VDSGPRKRPS RGGFTDKERQ 100
    DHRRRKALEN KRKQLSAGGK NLSKEEEEEL RRLTEEDERR ERRIAGPQVG 150
    GVNPLEGGTR GAPGGGFVPS MQGVPESPFT RTGEGLDIRG SQGFPWDILF 200
    PADPPSSPQS CRPQ 214
    Length:214
    Mass (Da):24,060
    Last modified:March 1, 2004 - v2
    Checksum:i4A4C6AAAF90A388E
    GO

    RNA editingi

    Partially edited. RNA editing at this position occurs on the antigenomic strand and consists of a conversion of A to G catalyzed by the cellular enzyme ADAR1. The unedited RNA version gives rise to the small delta antigen (AC P0C6L3), which ends with a nonsense codon at position 196. In the edited version, this amber codon is modified to a tryptophan codon and gives rise to the large delta antigen protein. S-HDAg suppresses editing of non-replicating antigenomic RNA, thereby regulating the extent of editing By similarity.By similarity

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D01075 Genomic RNA. Translation: BAA00874.1. Different termination.

    Keywords - Coding sequence diversityi

    RNA editing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D01075 Genomic RNA. Translation: BAA00874.1 . Different termination.

    3D structure databases

    ProteinModelPortali P29996.
    SMRi P29996. Positions 12-60.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    MINTi MINT-196884.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 4.10.220.40. 1 hit.
    InterProi IPR027403. Delta_antigen_N.
    IPR002506. HDV_ag.
    [Graphical view ]
    Pfami PF01517. HDV_ag. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and sequencing of RNA of hepatitis delta virus isolated from human serum."
      Saldanha J.A., Thomas H.C., Monjardino J.P.
      J. Gen. Virol. 71:1603-1606(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    2. "Characterization of nuclear targeting signal of hepatitis delta antigen: nuclear transport as a protein complex."
      Xia Y.-P., Yeh C.-T., Ou J.-H., Lai M.M.C.
      J. Virol. 66:914-921(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEAR LOCALIZATION SIGNAL, SUBCELLULAR LOCATION.
    3. "Isoprenylation mediates direct protein-protein interactions between hepatitis large delta antigen and hepatitis B virus surface antigen."
      Hwang S.B., Lai M.M.
      J. Virol. 67:7659-7662(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: ISOPRENYLATION AT CYS-211, INTERACTION WITH HBV HBSAG.
    4. "The hepatitis delta virus large antigen is farnesylated both in vitro and in animal cells."
      Otto J.C., Casey P.J.
      J. Biol. Chem. 271:4569-4572(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: ISOPRENYLATION AT CYS-211.
    5. "Characterization of the phosphorylated forms and the phosphorylated residues of hepatitis delta virus delta antigens."
      Mu J.J., Wu H.L., Chiang B.L., Chang R.P., Chen D.S., Chen P.J.
      J. Virol. 73:10540-10545(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION.
    6. "Large hepatitis delta antigen is not a suppressor of hepatitis delta virus RNA synthesis once RNA replication is established."
      Macnaughton T.B., Lai M.M.
      J. Virol. 76:9910-9919(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "Determination of the multimerization state of the hepatitis delta virus antigens in vivo."
      Cornillez-Ty C.T., Lazinski D.W.
      J. Virol. 77:10314-10326(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBUNIT.
    8. "Hepatitis D."
      Husa P., Linhartova A., Nemecek V., Husova L.
      Acta Virol. 49:219-225(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW.
    9. "Post-translational modification of delta antigen of hepatitis D virus."
      Huang W.H., Chen C.W., Wu H.L., Chen P.J.
      Curr. Top. Microbiol. Immunol. 307:91-112(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW.
    10. "Large hepatitis delta antigen is a novel clathrin adaptor-like protein."
      Huang C., Chang S.C., Yu I.C., Tsay Y.G., Chang M.F.
      J. Virol. 81:5985-5994(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    11. "Characterization of the nuclear localization signal of the hepatitis delta virus antigen."
      Alves C., Freitas N., Cunha C.
      Virology 370:12-21(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEAR LOCALIZATION SIGNAL, RNA-BINDING.

    Entry informationi

    Entry nameiLHDAG_HDVD3
    AccessioniPrimary (citable) accession number: P29996
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1993
    Last sequence update: March 1, 2004
    Last modified: October 1, 2014
    This is version 64 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3