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P29996 (LHDAG_HDVD3) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 63. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Large delta antigen

Short name=L-HDAg
Alternative name(s):
p27
OrganismHepatitis delta virus genotype I (isolate D380) (HDV) [Reference proteome]
Taxonomic identifier31762 [NCBI]
Taxonomic lineageVirusesDeltavirus
Virus hostHomo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length214 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Following virus entry into host cell, provides nuclear import of HDV RNPs thanks to its nuclear localization signal. Needs co-infection with hepatitis B virus to provide surface proteins, otherwise there is no packaging or budding. Packages the HDV ribonucleoprotein in hepatitis B virus empty particles. Interacts with both HDV genomic RNA and cytoplasmic tail of HBsAg. May inhibit viral RNA replication. Ref.6 Ref.10

Subunit structure

Homodimer. Homooctamer. Interacts with HBV HBsAg. May interact with clathrin to induce virion budding. Ref.3 Ref.7

Subcellular location

Virion. Host nucleushost nucleolus. Note: isoprenylated in the cytoplasm, and translocates in the nucleus possibly after phosphorylation. Translocates after to nuclear speckle, then to the ER membrane where interaction with Hepatitis B virus antigene takes place. Ref.2

Post-translational modification

Prenylated by host farnesyl-transferase in the cytoplasm prior to nucleus translocation.

Phosphorylated at serines by host CK2 and other kinases. phosphorylation does not seem to be important for its function. Ref.5

Sequence similarities

Belongs to the hepatitis delta antigen family.

RNA editing

Edited at position 196.
Partially edited. RNA editing at this position occurs on the antigenomic strand and consists of a conversion of A to G catalyzed by the cellular enzyme ADAR1. The unedited RNA version gives rise to the small delta antigen (AC P0C6L3), which ends with a nonsense codon at position 196. In the edited version, this amber codon is modified to a tryptophan codon and gives rise to the large delta antigen protein. S-HDAg suppresses editing of non-replicating antigenomic RNA, thereby regulating the extent of editing By similarity.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 211211Large delta antigen
PRO_0000038130
Propeptide212 – 2143Removed in mature form Probable
PRO_0000396787

Regions

Region12 – 6049Dimerization Potential
Region97 – 10711RNA-binding Potential
Region136 – 14611RNA-binding Potential
Motif66 – 7510Nuclear localization signal By similarity

Amino acid modifications

Modified residue21Phosphoserine; by host
Modified residue131Omega-N-methylated arginine; by host
Modified residue721N6-acetyllysine; by host
Modified residue1231Phosphoserine; by host
Modified residue1771Phosphoserine; by host
Modified residue2111Cysteine methyl ester; by host Probable
Lipidation2111S-farnesyl cysteine; by host Ref.3 Ref.4

Sequences

Sequence LengthMass (Da)Tools
P29996 [UniParc].

Last modified March 1, 2004. Version 2.
Checksum: 4A4C6AAAF90A388E

FASTA21424,060
        10         20         30         40         50         60 
MSRPEGRKNR GGREEVLEQW VSGRKKLEEL ERDLRKVKKK IKKLEDEHPW LGNIKGILGK 

        70         80         90        100        110        120 
KDKDGEGAPP AKRARTDQME VDSGPRKRPS RGGFTDKERQ DHRRRKALEN KRKQLSAGGK 

       130        140        150        160        170        180 
NLSKEEEEEL RRLTEEDERR ERRIAGPQVG GVNPLEGGTR GAPGGGFVPS MQGVPESPFT 

       190        200        210 
RTGEGLDIRG SQGFPWDILF PADPPSSPQS CRPQ 

« Hide

References

[1]"Cloning and sequencing of RNA of hepatitis delta virus isolated from human serum."
Saldanha J.A., Thomas H.C., Monjardino J.P.
J. Gen. Virol. 71:1603-1606(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]"Characterization of nuclear targeting signal of hepatitis delta antigen: nuclear transport as a protein complex."
Xia Y.-P., Yeh C.-T., Ou J.-H., Lai M.M.C.
J. Virol. 66:914-921(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEAR LOCALIZATION SIGNAL, SUBCELLULAR LOCATION.
[3]"Isoprenylation mediates direct protein-protein interactions between hepatitis large delta antigen and hepatitis B virus surface antigen."
Hwang S.B., Lai M.M.
J. Virol. 67:7659-7662(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: ISOPRENYLATION AT CYS-211, INTERACTION WITH HBV HBSAG.
[4]"The hepatitis delta virus large antigen is farnesylated both in vitro and in animal cells."
Otto J.C., Casey P.J.
J. Biol. Chem. 271:4569-4572(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: ISOPRENYLATION AT CYS-211.
[5]"Characterization of the phosphorylated forms and the phosphorylated residues of hepatitis delta virus delta antigens."
Mu J.J., Wu H.L., Chiang B.L., Chang R.P., Chen D.S., Chen P.J.
J. Virol. 73:10540-10545(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION.
[6]"Large hepatitis delta antigen is not a suppressor of hepatitis delta virus RNA synthesis once RNA replication is established."
Macnaughton T.B., Lai M.M.
J. Virol. 76:9910-9919(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[7]"Determination of the multimerization state of the hepatitis delta virus antigens in vivo."
Cornillez-Ty C.T., Lazinski D.W.
J. Virol. 77:10314-10326(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBUNIT.
[8]"Hepatitis D."
Husa P., Linhartova A., Nemecek V., Husova L.
Acta Virol. 49:219-225(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW.
[9]"Post-translational modification of delta antigen of hepatitis D virus."
Huang W.H., Chen C.W., Wu H.L., Chen P.J.
Curr. Top. Microbiol. Immunol. 307:91-112(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW.
[10]"Large hepatitis delta antigen is a novel clathrin adaptor-like protein."
Huang C., Chang S.C., Yu I.C., Tsay Y.G., Chang M.F.
J. Virol. 81:5985-5994(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[11]"Characterization of the nuclear localization signal of the hepatitis delta virus antigen."
Alves C., Freitas N., Cunha C.
Virology 370:12-21(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEAR LOCALIZATION SIGNAL, RNA-BINDING.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D01075 Genomic RNA. Translation: BAA00874.1. Different termination.

3D structure databases

ProteinModelPortalP29996.
SMRP29996. Positions 12-60.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

MINTMINT-196884.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D4.10.220.40. 1 hit.
InterProIPR027403. Delta_antigen_N.
IPR002506. HDV_ag.
[Graphical view]
PfamPF01517. HDV_ag. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameLHDAG_HDVD3
AccessionPrimary (citable) accession number: P29996
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: March 1, 2004
Last modified: February 19, 2014
This is version 63 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families