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P29996

- LHDAG_HDVD3

UniProt

P29996 - LHDAG_HDVD3

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Protein
Large delta antigen
Gene
N/A
Organism
Hepatitis delta virus genotype I (isolate D380) (HDV)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Following virus entry into host cell, provides nuclear import of HDV RNPs thanks to its nuclear localization signal. Needs co-infection with hepatitis B virus to provide surface proteins, otherwise there is no packaging or budding. Packages the HDV ribonucleoprotein in hepatitis B virus empty particles. Interacts with both HDV genomic RNA and cytoplasmic tail of HBsAg. May inhibit viral RNA replication.2 Publications

GO - Molecular functioni

  1. RNA binding Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. viral entry into host cell Source: UniProtKB-KW
  2. viral penetration into host nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Viral penetration into host nucleus, Virus entry into host cell

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Large delta antigen
Short name:
L-HDAg
Alternative name(s):
p27
OrganismiHepatitis delta virus genotype I (isolate D380) (HDV)
Taxonomic identifieri31762 [NCBI]
Taxonomic lineageiVirusesDeltavirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
ProteomesiUP000006675: Genome

Subcellular locationi

Virion. Host nucleushost nucleolus
Note: isoprenylated in the cytoplasm, and translocates in the nucleus possibly after phosphorylation. Translocates after to nuclear speckle, then to the ER membrane where interaction with Hepatitis B virus antigene takes place.1 Publication

GO - Cellular componenti

  1. host cell nucleolus Source: UniProtKB-SubCell
  2. intracellular Source: GOC
  3. virion Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Host nucleus, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 211211Large delta antigen
PRO_0000038130Add
BLAST
Propeptidei212 – 2143Removed in mature form Inferred
PRO_0000396787

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21Phosphoserine; by host
Modified residuei13 – 131Omega-N-methylated arginine; by host
Modified residuei72 – 721N6-acetyllysine; by host
Modified residuei123 – 1231Phosphoserine; by host
Modified residuei177 – 1771Phosphoserine; by host
Modified residuei211 – 2111Cysteine methyl ester; by host Inferred
Lipidationi211 – 2111S-farnesyl cysteine; by host2 Publications

Post-translational modificationi

Prenylated by host farnesyl-transferase in the cytoplasm prior to nucleus translocation.
Phosphorylated at serines by host CK2 and other kinases. phosphorylation does not seem to be important for its function.1 Publication

Keywords - PTMi

Acetylation, Lipoprotein, Methylation, Phosphoprotein, Prenylation

Interactioni

Subunit structurei

Homodimer. Homooctamer. Interacts with HBV HBsAg. May interact with clathrin to induce virion budding.2 Publications

Protein-protein interaction databases

MINTiMINT-196884.

Structurei

3D structure databases

ProteinModelPortaliP29996.
SMRiP29996. Positions 12-60.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni12 – 6049Dimerization Reviewed prediction
Add
BLAST
Regioni97 – 10711RNA-binding Reviewed prediction
Add
BLAST
Regioni136 – 14611RNA-binding Reviewed prediction
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi66 – 7510Nuclear localization signal By similarity

Sequence similaritiesi

Family and domain databases

Gene3Di4.10.220.40. 1 hit.
InterProiIPR027403. Delta_antigen_N.
IPR002506. HDV_ag.
[Graphical view]
PfamiPF01517. HDV_ag. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P29996-1 [UniParc]FASTAAdd to Basket

« Hide

MSRPEGRKNR GGREEVLEQW VSGRKKLEEL ERDLRKVKKK IKKLEDEHPW    50
LGNIKGILGK KDKDGEGAPP AKRARTDQME VDSGPRKRPS RGGFTDKERQ 100
DHRRRKALEN KRKQLSAGGK NLSKEEEEEL RRLTEEDERR ERRIAGPQVG 150
GVNPLEGGTR GAPGGGFVPS MQGVPESPFT RTGEGLDIRG SQGFPWDILF 200
PADPPSSPQS CRPQ 214
Length:214
Mass (Da):24,060
Last modified:March 1, 2004 - v2
Checksum:i4A4C6AAAF90A388E
GO

RNA editingi

Partially edited. RNA editing at this position occurs on the antigenomic strand and consists of a conversion of A to G catalyzed by the cellular enzyme ADAR1. The unedited RNA version gives rise to the small delta antigen (AC P0C6L3), which ends with a nonsense codon at position 196. In the edited version, this amber codon is modified to a tryptophan codon and gives rise to the large delta antigen protein. S-HDAg suppresses editing of non-replicating antigenomic RNA, thereby regulating the extent of editing By similarity.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D01075 Genomic RNA. Translation: BAA00874.1. Different termination.

Keywords - Coding sequence diversityi

RNA editing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D01075 Genomic RNA. Translation: BAA00874.1 . Different termination.

3D structure databases

ProteinModelPortali P29996.
SMRi P29996. Positions 12-60.
ModBasei Search...

Protein-protein interaction databases

MINTi MINT-196884.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 4.10.220.40. 1 hit.
InterProi IPR027403. Delta_antigen_N.
IPR002506. HDV_ag.
[Graphical view ]
Pfami PF01517. HDV_ag. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and sequencing of RNA of hepatitis delta virus isolated from human serum."
    Saldanha J.A., Thomas H.C., Monjardino J.P.
    J. Gen. Virol. 71:1603-1606(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "Characterization of nuclear targeting signal of hepatitis delta antigen: nuclear transport as a protein complex."
    Xia Y.-P., Yeh C.-T., Ou J.-H., Lai M.M.C.
    J. Virol. 66:914-921(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEAR LOCALIZATION SIGNAL, SUBCELLULAR LOCATION.
  3. "Isoprenylation mediates direct protein-protein interactions between hepatitis large delta antigen and hepatitis B virus surface antigen."
    Hwang S.B., Lai M.M.
    J. Virol. 67:7659-7662(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: ISOPRENYLATION AT CYS-211, INTERACTION WITH HBV HBSAG.
  4. "The hepatitis delta virus large antigen is farnesylated both in vitro and in animal cells."
    Otto J.C., Casey P.J.
    J. Biol. Chem. 271:4569-4572(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: ISOPRENYLATION AT CYS-211.
  5. "Characterization of the phosphorylated forms and the phosphorylated residues of hepatitis delta virus delta antigens."
    Mu J.J., Wu H.L., Chiang B.L., Chang R.P., Chen D.S., Chen P.J.
    J. Virol. 73:10540-10545(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION.
  6. "Large hepatitis delta antigen is not a suppressor of hepatitis delta virus RNA synthesis once RNA replication is established."
    Macnaughton T.B., Lai M.M.
    J. Virol. 76:9910-9919(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Determination of the multimerization state of the hepatitis delta virus antigens in vivo."
    Cornillez-Ty C.T., Lazinski D.W.
    J. Virol. 77:10314-10326(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT.
  8. "Hepatitis D."
    Husa P., Linhartova A., Nemecek V., Husova L.
    Acta Virol. 49:219-225(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.
  9. "Post-translational modification of delta antigen of hepatitis D virus."
    Huang W.H., Chen C.W., Wu H.L., Chen P.J.
    Curr. Top. Microbiol. Immunol. 307:91-112(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.
  10. "Large hepatitis delta antigen is a novel clathrin adaptor-like protein."
    Huang C., Chang S.C., Yu I.C., Tsay Y.G., Chang M.F.
    J. Virol. 81:5985-5994(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  11. "Characterization of the nuclear localization signal of the hepatitis delta virus antigen."
    Alves C., Freitas N., Cunha C.
    Virology 370:12-21(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEAR LOCALIZATION SIGNAL, RNA-BINDING.

Entry informationi

Entry nameiLHDAG_HDVD3
AccessioniPrimary (citable) accession number: P29996
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: March 1, 2004
Last modified: February 19, 2014
This is version 63 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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