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Protein

Inositol 1,4,5-trisphosphate receptor type 2

Gene

Itpr2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Receptor for inositol 1,4,5-trisphosphate, a second messenger that mediates the release of intracellular calcium. This release is regulated by cAMP both dependently and independently of PKA (By similarity).By similarity

GO - Molecular functioni

  • calcium ion binding Source: RGD
  • inositol 1,4,5 trisphosphate binding Source: RGD
  • inositol 1,4,5-trisphosphate-sensitive calcium-release channel activity Source: RGD
  • phosphatidylinositol binding Source: GO_Central

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Calcium channel, Ion channel, Ligand-gated ion channel, Receptor

Keywords - Biological processi

Calcium transport, Ion transport, Transport

Keywords - Ligandi

Calcium

Enzyme and pathway databases

ReactomeiR-RNO-112043. PLC beta mediated events.
R-RNO-1489509. DAG and IP3 signaling.
R-RNO-2029485. Role of phospholipids in phagocytosis.
R-RNO-2871809. FCERI mediated Ca+2 mobilization.
R-RNO-4086398. Ca2+ pathway.
R-RNO-422356. Regulation of insulin secretion.
R-RNO-5218921. VEGFR2 mediated cell proliferation.
R-RNO-5578775. Ion homeostasis.
R-RNO-5607763. CLEC7A (Dectin-1) induces NFAT activation.
R-RNO-983695. Antigen activates B Cell Receptor (BCR) leading to generation of second messengers.

Protein family/group databases

TCDBi1.A.3.2.1. the ryanodine-inositol 1,4,5-triphosphate receptor ca(2+) channel (rir-cac) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Inositol 1,4,5-trisphosphate receptor type 2
Alternative name(s):
IP3 receptor isoform 2
Short name:
IP3R 2
Short name:
InsP3R2
Type 2 inositol 1,4,5-trisphosphate receptor
Short name:
Type 2 InsP3 receptor
Gene namesi
Name:Itpr2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi69649. Itpr2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 2227CytoplasmicSequence analysisAdd BLAST2227
Transmembranei2228 – 2248HelicalSequence analysisAdd BLAST21
Topological domaini2249 – 2260ExtracellularSequence analysisAdd BLAST12
Transmembranei2261 – 2281HelicalSequence analysisAdd BLAST21
Topological domaini2282 – 2284CytoplasmicSequence analysis3
Transmembranei2285 – 2305HelicalSequence analysisAdd BLAST21
Topological domaini2306 – 2307ExtracellularSequence analysis2
Transmembranei2308 – 2328HelicalSequence analysisAdd BLAST21
Topological domaini2329 – 2351CytoplasmicSequence analysisAdd BLAST23
Transmembranei2352 – 2372HelicalSequence analysisAdd BLAST21
Topological domaini2373 – 2394ExtracellularSequence analysisAdd BLAST22
Transmembranei2395 – 2415HelicalSequence analysisAdd BLAST21
Topological domaini2416 – 2521CytoplasmicSequence analysisAdd BLAST106
Transmembranei2522 – 2542HelicalSequence analysisAdd BLAST21
Topological domaini2543 – 2701ExtracellularSequence analysisAdd BLAST159

GO - Cellular componenti

  • axon Source: RGD
  • cytoplasm Source: RGD
  • endoplasmic reticulum Source: RGD
  • endoplasmic reticulum membrane Source: RGD
  • integral component of membrane Source: UniProtKB-KW
  • myelin sheath Source: RGD
  • plasma membrane Source: GO_Central
  • sarcoplasmic reticulum Source: RGD
  • secretory granule membrane Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001539261 – 2701Inositol 1,4,5-trisphosphate receptor type 2Add BLAST2701

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei937Phosphoserine; by PKABy similarity1
Modified residuei1160PhosphoserineBy similarity1
Modified residuei1709PhosphoserineBy similarity1
Modified residuei2607PhosphotyrosineSequence analysis1
Modified residuei2633PhosphoserineBy similarity1
Modified residuei2636PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylation by cAMP-dependent PKA on Ser-937 increases calcium release.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP29995.
PRIDEiP29995.

PTM databases

iPTMnetiP29995.
PhosphoSitePlusiP29995.

Interactioni

Subunit structurei

Homotetramer. Interacts with CABP1. Interacts with BOK; regulates ITPR2 expression (PubMed:23884412).2 Publications

Protein-protein interaction databases

BioGridi249575. 2 interactors.
STRINGi10116.ENSRNOP00000047905.

Structurei

3D structure databases

ProteinModelPortaliP29995.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini112 – 166MIR 1PROSITE-ProRule annotationAdd BLAST55
Domaini173 – 223MIR 2PROSITE-ProRule annotationAdd BLAST51
Domaini231 – 287MIR 3PROSITE-ProRule annotationAdd BLAST57
Domaini294 – 357MIR 4PROSITE-ProRule annotationAdd BLAST64
Domaini378 – 434MIR 5PROSITE-ProRule annotationAdd BLAST57

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni265 – 269Inositol 1,4,5-trisphosphate bindingBy similarity5
Regioni507 – 510Inositol 1,4,5-trisphosphate bindingBy similarity4
Regioni567 – 569Inositol 1,4,5-trisphosphate bindingBy similarity3

Domaini

The receptor contains a calcium channel in its C-terminal extremity. Its large N-terminal cytoplasmic region has the ligand-binding site in the N-terminus and modulatory sites in the middle portion immediately upstream of the channel region.

Sequence similaritiesi

Belongs to the InsP3 receptor family.Curated
Contains 5 MIR domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3533. Eukaryota.
ENOG410XR97. LUCA.
HOGENOMiHOG000007660.
HOVERGENiHBG052158.
InParanoidiP29995.
KOiK04959.
PhylomeDBiP29995.

Family and domain databases

Gene3Di1.25.10.30. 2 hits.
InterProiIPR016024. ARM-type_fold.
IPR014821. Ins145_P3_rcpt.
IPR000493. InsP3_rcpt-bd.
IPR005821. Ion_trans_dom.
IPR016093. MIR_motif.
IPR013662. RIH_assoc-dom.
IPR000699. RIH_dom.
IPR015925. Ryanodine_recept-rel.
[Graphical view]
PANTHERiPTHR13715. PTHR13715. 1 hit.
PfamiPF08709. Ins145_P3_rec. 1 hit.
PF00520. Ion_trans. 1 hit.
PF02815. MIR. 1 hit.
PF08454. RIH_assoc. 1 hit.
PF01365. RYDR_ITPR. 2 hits.
[Graphical view]
PRINTSiPR00779. INSP3RECEPTR.
SMARTiSM00472. MIR. 4 hits.
[Graphical view]
SUPFAMiSSF100909. SSF100909. 2 hits.
SSF48371. SSF48371. 3 hits.
SSF82109. SSF82109. 2 hits.
PROSITEiPS50919. MIR. 5 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P29995-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDKMSSFLY IGDIVSLYAE GSVNGFISTL GLVDDRCVVH PEAGDLTNPP
60 70 80 90 100
KKFRDCLFKV CPMNRYSAQK QYWKAKQAKQ GNHTEAALLK KLQHAAELEQ
110 120 130 140 150
KQNESENRKL LGEIVKYSKV IQLLHIKSNK YLTVNKRLPA LLEKNAMRVS
160 170 180 190 200
LDAAGNEGSW FYIHPFWKLR SEGDNIVVGD KVVLMPVNAG QPLHASNVEL
210 220 230 240 250
LDNPGCKEVN AVNCNTSWKI TLFMKFSSYR EDVLKGGDVV RLFHAEQEKF
260 270 280 290 300
LTCDDYEKKQ HIFLRTTLRQ SATSATSSKA LWEIEVVHHD PCRGGAGQWN
310 320 330 340 350
SLFRFKHLAT GNYLAAELNP DYRDAQNEGK TVRDGELPTS KKKHQAGEKI
360 370 380 390 400
MYTLVSVPHG NDIASLFELD ATTLQRADCL VPRNSYVRLR HLCTNTWVTS
410 420 430 440 450
TSIPIDTEEE RPVMLKIGTC QTKEDKEAFA IVCVPLSEVR DLDFANDANK
460 470 480 490 500
VLATTVKKLE NGSITQNERR FVTKLLEDLI FFVADVTNNG QDVLDVVITK
510 520 530 540 550
PNRERQKLMR EQNILAQVFG ILKAPFKEKA GEGSMLRLED LGDQRYAPYK
560 570 580 590 600
YVLRLCYRVL RHSQQDYRKN QEYIAKNFCV MQSQIGYDIL AEDTITALLH
610 620 630 640 650
NNRKLLEKHI TAKEIETFVS LLRRNREPRF LDYLSDLCVS NSTAIPVTQE
660 670 680 690 700
LICKFMLSPG NADILIQTKL VSMQVENPME SSILPDDIDD EEVWLYWIDS
710 720 730 740 750
NKEPHGKAIR HLAQEAREGT KADLEVLTYY RYQLNLFARM CLDRQYLAIN
760 770 780 790 800
QISTQLSVDL ILRCVSDESL PFDLRASFCR LMLHMHVDRD PQESVVPVRY
810 820 830 840 850
ARLWTEIPTK ITIHEYDSIT DSSRNDMKRK FALTMEFVEE YLKEVVNQPF
860 870 880 890 900
PFGDKEKNKL TFEVVHLARN LIYFGFYSFS ELLRLTRTLL AILDIVQAPM
910 920 930 940 950
SSYFERLSKF QDGSNNVMRT IHGVGEMMTQ MVLSRGSIFP VSWPDAQPSV
960 970 980 990 1000
HPSKQASPGE QEDVTVMDTK LKVIEILQFI LSVRLDYRIS YMLSIYKKEF
1010 1020 1030 1040 1050
GENDGNGDPS ASGTPETLLP SALVPDIDEI AAQAETMFAG RKEKTPVQLD
1060 1070 1080 1090 1100
DEGGRTFLRV LIHLIMHDYA PLLSGALQLL FKHFSQRAEV LQAFKQVQLL
1110 1120 1130 1140 1150
VSNQDVDNYK QIKADLDQLR LTVEKSELWV EKSGSYENGD MGEGQAKGGE
1160 1170 1180 1190 1200
EANEESNLLS PVQDGAKTPQ IDSNKGNNYR IVKEILIRLS KLCVQNKKCR
1210 1220 1230 1240 1250
NQHQRLLKNM GAHSVVLDLL QIPYEKTDEK MNEVMDLAHT FLQNFCRGNP
1260 1270 1280 1290 1300
QNQVLLHKHL NLFLTPGLLE AETMRHIFMN NYHLCNEISE RVVQHFVHCI
1310 1320 1330 1340 1350
ETHGRHVEYL RFLQTIVKAD GKYVKKCQDM VMTELINGGE DVLIFYNDRA
1360 1370 1380 1390 1400
SFPILLNMMC SERARGDESG PLAYHITLVE LLAACTEGKN VYTEIKCNSL
1410 1420 1430 1440 1450
LPLDDIVRVV THDDCIPEVK IAYVNFVNHC YVDTEVEMKE IYTSNHIWKL
1460 1470 1480 1490 1500
FENFLVDMAR VCNTTTDRKH ADTFLERCVT ESVMNIVSGF FNSPFSDNST
1510 1520 1530 1540 1550
SLQTHQPVFI QLLQSAFRIY NCTWPNPAQK ASVESCIRAL AEVAKNRGIA
1560 1570 1580 1590 1600
IPVDLDSQVN TLFMKNHSST VQRAAMGWRL SARSGPRFKE ALGGPAWDYR
1610 1620 1630 1640 1650
NIIEKLQDVV ASLEQQFSPM MQAEFSVLVD VLYSPELLFP EGSDARIRCG
1660 1670 1680 1690 1700
AFMSKLINHT KKLMEKEEKL CIKILQTLRE MLEKKDSFME ESSTLRKILL
1710 1720 1730 1740 1750
NRYFKGDHSV GVNGPLSGAY AKTAQVGGGF TGQDADKTGI SMSDIQCLLD
1760 1770 1780 1790 1800
KEGASELVID VIVNTKNDRI FSEGILLGIA LLEGGNTQTQ NSFYQQLHEQ
1810 1820 1830 1840 1850
KKSEKFFKVL YDRMKAAQKE IRSTVTVNTI DLGSKKREED SDLMALGPRM
1860 1870 1880 1890 1900
RVRDSSLHLK EGMKGQLTEA SSATSKAYCV YRREMDPDID TMCPGQEAGS
1910 1920 1930 1940 1950
AEEKSAEEVT MSPAITIMRP ILRFLQLLCE NHNRELQNFL RNQNNKTNYN
1960 1970 1980 1990 2000
LVCETLQFLD CICGSTTGGL GLLGLYINEK NVALVNQTLE SLTEYCQGPC
2010 2020 2030 2040 2050
HENQTCIATH ESNGIDIIIA LILSDINPLG KYRMDLVLQL KNNASKLLLA
2060 2070 2080 2090 2100
IMESRHDSEN AERILFNMRP KELVDVMKNA YNQGLECNHG DEEGGDDGVS
2110 2120 2130 2140 2150
PKDVGHNIYI LAHQLARHNK LLQQMLKPGS DPEEGDEALK YYANHTAQIE
2160 2170 2180 2190 2200
IVRHDRTMEQ IVFPVPNICE FLTRESKYRV FNTTERDEQG SKVNDFFQQT
2210 2220 2230 2240 2250
EDLYNEMKWQ KKIRNNPALF WFSRHISLWG SISFNLAVFI NLAVALFYPF
2260 2270 2280 2290 2300
GDDGDEGTLS PLFSALLWVA VAICTSMLFF FSKPVGIRPF LVSIMLRSIY
2310 2320 2330 2340 2350
TIGLGPTLIL LGAANLCNKI VFLVSFVGNR GTFTRGYRAV ILDMAFLYHV
2360 2370 2380 2390 2400
AYVLVCMLGL FVHEFFYSFL LFDLVYREET LLNVIKSVTR NGRSIILTAV
2410 2420 2430 2440 2450
LALILVYLFS IIGFLFLKDD FTMEVDRLKN RTPVTGNDGV PTMTLTSMLG
2460 2470 2480 2490 2500
TCPKENCSPT IPSSNAAGEG GEDGIERTCD TLLMCIVTVL NQGLRNGGGV
2510 2520 2530 2540 2550
GDVLRRPSKD EPLFAARVVY DLLFFFIVII IVLNLIFGVI IDTFADLRSE
2560 2570 2580 2590 2600
KQKKEKILKT TCFICGLERD KFDNKTVSFE EHIKSEHNMW HYLYFIVLVK
2610 2620 2630 2640 2650
VKDPTEYTGP ESYVAQMITE KNLDWFPRMR AMSLVSNEGD SEQNEIRNLQ
2660 2670 2680 2690 2700
EKLESTMSLV KQLSGQLAEL KEQMTEQRKN KQRLGFLGSN TPHENHHMPP

H
Length:2,701
Mass (Da):307,058
Last modified:April 1, 1993 - v1
Checksum:i42BF7F1024335984
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti119K → N in AAK11622 (Ref. 2) Curated1
Sequence conflicti344H → R in AAK11622 (Ref. 2) Curated1
Sequence conflicti943W → V in AAK11622 (Ref. 2) Curated1
Sequence conflicti1692S → G in AAK11622 (Ref. 2) Curated1
Sequence conflicti2556K → E in AAK11622 (Ref. 2) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti689D → H.1
Natural varianti1013G → C.1
Natural varianti1256L → P.1
Natural varianti2384V → I.1
Natural varianti2694E → V.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X61677 mRNA. Translation: CAA43852.1.
AF329470 mRNA. Translation: AAK11622.1.
PIRiS17796.
RefSeqiNP_112308.1. NM_031046.3.
UniGeneiRn.89152.

Genome annotation databases

GeneIDi81678.
KEGGirno:81678.
UCSCiRGD:69649. rat.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X61677 mRNA. Translation: CAA43852.1.
AF329470 mRNA. Translation: AAK11622.1.
PIRiS17796.
RefSeqiNP_112308.1. NM_031046.3.
UniGeneiRn.89152.

3D structure databases

ProteinModelPortaliP29995.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi249575. 2 interactors.
STRINGi10116.ENSRNOP00000047905.

Protein family/group databases

TCDBi1.A.3.2.1. the ryanodine-inositol 1,4,5-triphosphate receptor ca(2+) channel (rir-cac) family.

PTM databases

iPTMnetiP29995.
PhosphoSitePlusiP29995.

Proteomic databases

PaxDbiP29995.
PRIDEiP29995.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi81678.
KEGGirno:81678.
UCSCiRGD:69649. rat.

Organism-specific databases

CTDi3709.
RGDi69649. Itpr2.

Phylogenomic databases

eggNOGiKOG3533. Eukaryota.
ENOG410XR97. LUCA.
HOGENOMiHOG000007660.
HOVERGENiHBG052158.
InParanoidiP29995.
KOiK04959.
PhylomeDBiP29995.

Enzyme and pathway databases

ReactomeiR-RNO-112043. PLC beta mediated events.
R-RNO-1489509. DAG and IP3 signaling.
R-RNO-2029485. Role of phospholipids in phagocytosis.
R-RNO-2871809. FCERI mediated Ca+2 mobilization.
R-RNO-4086398. Ca2+ pathway.
R-RNO-422356. Regulation of insulin secretion.
R-RNO-5218921. VEGFR2 mediated cell proliferation.
R-RNO-5578775. Ion homeostasis.
R-RNO-5607763. CLEC7A (Dectin-1) induces NFAT activation.
R-RNO-983695. Antigen activates B Cell Receptor (BCR) leading to generation of second messengers.

Miscellaneous databases

PROiP29995.

Family and domain databases

Gene3Di1.25.10.30. 2 hits.
InterProiIPR016024. ARM-type_fold.
IPR014821. Ins145_P3_rcpt.
IPR000493. InsP3_rcpt-bd.
IPR005821. Ion_trans_dom.
IPR016093. MIR_motif.
IPR013662. RIH_assoc-dom.
IPR000699. RIH_dom.
IPR015925. Ryanodine_recept-rel.
[Graphical view]
PANTHERiPTHR13715. PTHR13715. 1 hit.
PfamiPF08709. Ins145_P3_rec. 1 hit.
PF00520. Ion_trans. 1 hit.
PF02815. MIR. 1 hit.
PF08454. RIH_assoc. 1 hit.
PF01365. RYDR_ITPR. 2 hits.
[Graphical view]
PRINTSiPR00779. INSP3RECEPTR.
SMARTiSM00472. MIR. 4 hits.
[Graphical view]
SUPFAMiSSF100909. SSF100909. 2 hits.
SSF48371. SSF48371. 3 hits.
SSF82109. SSF82109. 2 hits.
PROSITEiPS50919. MIR. 5 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiITPR2_RAT
AccessioniPrimary (citable) accession number: P29995
Secondary accession number(s): Q99P56
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: November 2, 2016
This is version 139 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.