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Protein

Inositol 1,4,5-trisphosphate receptor type 2

Gene

Itpr2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Receptor for inositol 1,4,5-trisphosphate, a second messenger that mediates the release of intracellular calcium. This release is regulated by cAMP both dependently and independently of PKA (By similarity).By similarity

GO - Molecular functioni

  • calcium ion binding Source: RGD
  • inositol 1,4,5 trisphosphate binding Source: RGD
  • inositol 1,4,5-trisphosphate-sensitive calcium-release channel activity Source: RGD

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Calcium channel, Ion channel, Ligand-gated ion channel, Receptor

Keywords - Biological processi

Calcium transport, Ion transport, Transport

Keywords - Ligandi

Calcium

Enzyme and pathway databases

ReactomeiR-RNO-112043. PLC beta mediated events.
R-RNO-1489509. DAG and IP3 signaling.
R-RNO-2029485. Role of phospholipids in phagocytosis.
R-RNO-2871809. FCERI mediated Ca+2 mobilization.
R-RNO-4086398. Ca2+ pathway.
R-RNO-422356. Regulation of insulin secretion.
R-RNO-5218921. VEGFR2 mediated cell proliferation.
R-RNO-5578775. Ion homeostasis.
R-RNO-5607763. CLEC7A (Dectin-1) induces NFAT activation.
R-RNO-983695. Antigen activates B Cell Receptor (BCR) leading to generation of second messengers.

Protein family/group databases

TCDBi1.A.3.2.1. the ryanodine-inositol 1,4,5-triphosphate receptor ca(2+) channel (rir-cac) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Inositol 1,4,5-trisphosphate receptor type 2
Alternative name(s):
IP3 receptor isoform 2
Short name:
IP3R 2
Short name:
InsP3R2
Type 2 inositol 1,4,5-trisphosphate receptor
Short name:
Type 2 InsP3 receptor
Gene namesi
Name:Itpr2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi69649. Itpr2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 22272227CytoplasmicSequence analysisAdd
BLAST
Transmembranei2228 – 224821HelicalSequence analysisAdd
BLAST
Topological domaini2249 – 226012ExtracellularSequence analysisAdd
BLAST
Transmembranei2261 – 228121HelicalSequence analysisAdd
BLAST
Topological domaini2282 – 22843CytoplasmicSequence analysis
Transmembranei2285 – 230521HelicalSequence analysisAdd
BLAST
Topological domaini2306 – 23072ExtracellularSequence analysis
Transmembranei2308 – 232821HelicalSequence analysisAdd
BLAST
Topological domaini2329 – 235123CytoplasmicSequence analysisAdd
BLAST
Transmembranei2352 – 237221HelicalSequence analysisAdd
BLAST
Topological domaini2373 – 239422ExtracellularSequence analysisAdd
BLAST
Transmembranei2395 – 241521HelicalSequence analysisAdd
BLAST
Topological domaini2416 – 2521106CytoplasmicSequence analysisAdd
BLAST
Transmembranei2522 – 254221HelicalSequence analysisAdd
BLAST
Topological domaini2543 – 2701159ExtracellularSequence analysisAdd
BLAST

GO - Cellular componenti

  • axon Source: RGD
  • cytoplasm Source: RGD
  • endoplasmic reticulum Source: RGD
  • endoplasmic reticulum membrane Source: RGD
  • integral component of membrane Source: UniProtKB-KW
  • myelin sheath Source: RGD
  • sarcoplasmic reticulum Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 27012701Inositol 1,4,5-trisphosphate receptor type 2PRO_0000153926Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei937 – 9371Phosphoserine; by PKABy similarity
Modified residuei1160 – 11601PhosphoserineBy similarity
Modified residuei1709 – 17091PhosphoserineBy similarity
Modified residuei2607 – 26071PhosphotyrosineSequence analysis
Modified residuei2633 – 26331PhosphoserineBy similarity
Modified residuei2636 – 26361PhosphoserineBy similarity

Post-translational modificationi

Phosphorylation by cAMP-dependent PKA on Ser-937 increases calcium release.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP29995.
PRIDEiP29995.

PTM databases

iPTMnetiP29995.

Interactioni

Subunit structurei

Homotetramer. Interacts with CABP1.1 Publication

Protein-protein interaction databases

BioGridi249575. 2 interactions.
STRINGi10116.ENSRNOP00000047905.

Structurei

3D structure databases

ProteinModelPortaliP29995.
SMRiP29995. Positions 7-225, 236-602.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini112 – 16655MIR 1PROSITE-ProRule annotationAdd
BLAST
Domaini173 – 22351MIR 2PROSITE-ProRule annotationAdd
BLAST
Domaini231 – 28757MIR 3PROSITE-ProRule annotationAdd
BLAST
Domaini294 – 35764MIR 4PROSITE-ProRule annotationAdd
BLAST
Domaini378 – 43457MIR 5PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni265 – 2695Inositol 1,4,5-trisphosphate bindingBy similarity
Regioni507 – 5104Inositol 1,4,5-trisphosphate bindingBy similarity
Regioni567 – 5693Inositol 1,4,5-trisphosphate bindingBy similarity

Domaini

The receptor contains a calcium channel in its C-terminal extremity. Its large N-terminal cytoplasmic region has the ligand-binding site in the N-terminus and modulatory sites in the middle portion immediately upstream of the channel region.

Sequence similaritiesi

Belongs to the InsP3 receptor family.Curated
Contains 5 MIR domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3533. Eukaryota.
ENOG410XR97. LUCA.
HOGENOMiHOG000007660.
HOVERGENiHBG052158.
InParanoidiP29995.
KOiK04959.
PhylomeDBiP29995.

Family and domain databases

Gene3Di1.25.10.30. 2 hits.
InterProiIPR016024. ARM-type_fold.
IPR014821. Ins145_P3_rcpt.
IPR000493. InsP3_rcpt-bd.
IPR005821. Ion_trans_dom.
IPR016093. MIR_motif.
IPR013662. RIH_assoc-dom.
IPR000699. RIH_dom.
IPR015925. Ryanodine_recept-rel.
[Graphical view]
PANTHERiPTHR13715. PTHR13715. 1 hit.
PfamiPF08709. Ins145_P3_rec. 1 hit.
PF00520. Ion_trans. 1 hit.
PF02815. MIR. 1 hit.
PF08454. RIH_assoc. 1 hit.
PF01365. RYDR_ITPR. 2 hits.
[Graphical view]
PRINTSiPR00779. INSP3RECEPTR.
SMARTiSM00472. MIR. 4 hits.
[Graphical view]
SUPFAMiSSF100909. SSF100909. 2 hits.
SSF48371. SSF48371. 3 hits.
SSF82109. SSF82109. 2 hits.
PROSITEiPS50919. MIR. 5 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P29995-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDKMSSFLY IGDIVSLYAE GSVNGFISTL GLVDDRCVVH PEAGDLTNPP
60 70 80 90 100
KKFRDCLFKV CPMNRYSAQK QYWKAKQAKQ GNHTEAALLK KLQHAAELEQ
110 120 130 140 150
KQNESENRKL LGEIVKYSKV IQLLHIKSNK YLTVNKRLPA LLEKNAMRVS
160 170 180 190 200
LDAAGNEGSW FYIHPFWKLR SEGDNIVVGD KVVLMPVNAG QPLHASNVEL
210 220 230 240 250
LDNPGCKEVN AVNCNTSWKI TLFMKFSSYR EDVLKGGDVV RLFHAEQEKF
260 270 280 290 300
LTCDDYEKKQ HIFLRTTLRQ SATSATSSKA LWEIEVVHHD PCRGGAGQWN
310 320 330 340 350
SLFRFKHLAT GNYLAAELNP DYRDAQNEGK TVRDGELPTS KKKHQAGEKI
360 370 380 390 400
MYTLVSVPHG NDIASLFELD ATTLQRADCL VPRNSYVRLR HLCTNTWVTS
410 420 430 440 450
TSIPIDTEEE RPVMLKIGTC QTKEDKEAFA IVCVPLSEVR DLDFANDANK
460 470 480 490 500
VLATTVKKLE NGSITQNERR FVTKLLEDLI FFVADVTNNG QDVLDVVITK
510 520 530 540 550
PNRERQKLMR EQNILAQVFG ILKAPFKEKA GEGSMLRLED LGDQRYAPYK
560 570 580 590 600
YVLRLCYRVL RHSQQDYRKN QEYIAKNFCV MQSQIGYDIL AEDTITALLH
610 620 630 640 650
NNRKLLEKHI TAKEIETFVS LLRRNREPRF LDYLSDLCVS NSTAIPVTQE
660 670 680 690 700
LICKFMLSPG NADILIQTKL VSMQVENPME SSILPDDIDD EEVWLYWIDS
710 720 730 740 750
NKEPHGKAIR HLAQEAREGT KADLEVLTYY RYQLNLFARM CLDRQYLAIN
760 770 780 790 800
QISTQLSVDL ILRCVSDESL PFDLRASFCR LMLHMHVDRD PQESVVPVRY
810 820 830 840 850
ARLWTEIPTK ITIHEYDSIT DSSRNDMKRK FALTMEFVEE YLKEVVNQPF
860 870 880 890 900
PFGDKEKNKL TFEVVHLARN LIYFGFYSFS ELLRLTRTLL AILDIVQAPM
910 920 930 940 950
SSYFERLSKF QDGSNNVMRT IHGVGEMMTQ MVLSRGSIFP VSWPDAQPSV
960 970 980 990 1000
HPSKQASPGE QEDVTVMDTK LKVIEILQFI LSVRLDYRIS YMLSIYKKEF
1010 1020 1030 1040 1050
GENDGNGDPS ASGTPETLLP SALVPDIDEI AAQAETMFAG RKEKTPVQLD
1060 1070 1080 1090 1100
DEGGRTFLRV LIHLIMHDYA PLLSGALQLL FKHFSQRAEV LQAFKQVQLL
1110 1120 1130 1140 1150
VSNQDVDNYK QIKADLDQLR LTVEKSELWV EKSGSYENGD MGEGQAKGGE
1160 1170 1180 1190 1200
EANEESNLLS PVQDGAKTPQ IDSNKGNNYR IVKEILIRLS KLCVQNKKCR
1210 1220 1230 1240 1250
NQHQRLLKNM GAHSVVLDLL QIPYEKTDEK MNEVMDLAHT FLQNFCRGNP
1260 1270 1280 1290 1300
QNQVLLHKHL NLFLTPGLLE AETMRHIFMN NYHLCNEISE RVVQHFVHCI
1310 1320 1330 1340 1350
ETHGRHVEYL RFLQTIVKAD GKYVKKCQDM VMTELINGGE DVLIFYNDRA
1360 1370 1380 1390 1400
SFPILLNMMC SERARGDESG PLAYHITLVE LLAACTEGKN VYTEIKCNSL
1410 1420 1430 1440 1450
LPLDDIVRVV THDDCIPEVK IAYVNFVNHC YVDTEVEMKE IYTSNHIWKL
1460 1470 1480 1490 1500
FENFLVDMAR VCNTTTDRKH ADTFLERCVT ESVMNIVSGF FNSPFSDNST
1510 1520 1530 1540 1550
SLQTHQPVFI QLLQSAFRIY NCTWPNPAQK ASVESCIRAL AEVAKNRGIA
1560 1570 1580 1590 1600
IPVDLDSQVN TLFMKNHSST VQRAAMGWRL SARSGPRFKE ALGGPAWDYR
1610 1620 1630 1640 1650
NIIEKLQDVV ASLEQQFSPM MQAEFSVLVD VLYSPELLFP EGSDARIRCG
1660 1670 1680 1690 1700
AFMSKLINHT KKLMEKEEKL CIKILQTLRE MLEKKDSFME ESSTLRKILL
1710 1720 1730 1740 1750
NRYFKGDHSV GVNGPLSGAY AKTAQVGGGF TGQDADKTGI SMSDIQCLLD
1760 1770 1780 1790 1800
KEGASELVID VIVNTKNDRI FSEGILLGIA LLEGGNTQTQ NSFYQQLHEQ
1810 1820 1830 1840 1850
KKSEKFFKVL YDRMKAAQKE IRSTVTVNTI DLGSKKREED SDLMALGPRM
1860 1870 1880 1890 1900
RVRDSSLHLK EGMKGQLTEA SSATSKAYCV YRREMDPDID TMCPGQEAGS
1910 1920 1930 1940 1950
AEEKSAEEVT MSPAITIMRP ILRFLQLLCE NHNRELQNFL RNQNNKTNYN
1960 1970 1980 1990 2000
LVCETLQFLD CICGSTTGGL GLLGLYINEK NVALVNQTLE SLTEYCQGPC
2010 2020 2030 2040 2050
HENQTCIATH ESNGIDIIIA LILSDINPLG KYRMDLVLQL KNNASKLLLA
2060 2070 2080 2090 2100
IMESRHDSEN AERILFNMRP KELVDVMKNA YNQGLECNHG DEEGGDDGVS
2110 2120 2130 2140 2150
PKDVGHNIYI LAHQLARHNK LLQQMLKPGS DPEEGDEALK YYANHTAQIE
2160 2170 2180 2190 2200
IVRHDRTMEQ IVFPVPNICE FLTRESKYRV FNTTERDEQG SKVNDFFQQT
2210 2220 2230 2240 2250
EDLYNEMKWQ KKIRNNPALF WFSRHISLWG SISFNLAVFI NLAVALFYPF
2260 2270 2280 2290 2300
GDDGDEGTLS PLFSALLWVA VAICTSMLFF FSKPVGIRPF LVSIMLRSIY
2310 2320 2330 2340 2350
TIGLGPTLIL LGAANLCNKI VFLVSFVGNR GTFTRGYRAV ILDMAFLYHV
2360 2370 2380 2390 2400
AYVLVCMLGL FVHEFFYSFL LFDLVYREET LLNVIKSVTR NGRSIILTAV
2410 2420 2430 2440 2450
LALILVYLFS IIGFLFLKDD FTMEVDRLKN RTPVTGNDGV PTMTLTSMLG
2460 2470 2480 2490 2500
TCPKENCSPT IPSSNAAGEG GEDGIERTCD TLLMCIVTVL NQGLRNGGGV
2510 2520 2530 2540 2550
GDVLRRPSKD EPLFAARVVY DLLFFFIVII IVLNLIFGVI IDTFADLRSE
2560 2570 2580 2590 2600
KQKKEKILKT TCFICGLERD KFDNKTVSFE EHIKSEHNMW HYLYFIVLVK
2610 2620 2630 2640 2650
VKDPTEYTGP ESYVAQMITE KNLDWFPRMR AMSLVSNEGD SEQNEIRNLQ
2660 2670 2680 2690 2700
EKLESTMSLV KQLSGQLAEL KEQMTEQRKN KQRLGFLGSN TPHENHHMPP

H
Length:2,701
Mass (Da):307,058
Last modified:April 1, 1993 - v1
Checksum:i42BF7F1024335984
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti119 – 1191K → N in AAK11622 (Ref. 2) Curated
Sequence conflicti344 – 3441H → R in AAK11622 (Ref. 2) Curated
Sequence conflicti943 – 9431W → V in AAK11622 (Ref. 2) Curated
Sequence conflicti1692 – 16921S → G in AAK11622 (Ref. 2) Curated
Sequence conflicti2556 – 25561K → E in AAK11622 (Ref. 2) Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti689 – 6891D → H.
Natural varianti1013 – 10131G → C.
Natural varianti1256 – 12561L → P.
Natural varianti2384 – 23841V → I.
Natural varianti2694 – 26941E → V.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X61677 mRNA. Translation: CAA43852.1.
AF329470 mRNA. Translation: AAK11622.1.
PIRiS17796.
RefSeqiNP_112308.1. NM_031046.3.
UniGeneiRn.89152.

Genome annotation databases

GeneIDi81678.
KEGGirno:81678.
UCSCiRGD:69649. rat.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X61677 mRNA. Translation: CAA43852.1.
AF329470 mRNA. Translation: AAK11622.1.
PIRiS17796.
RefSeqiNP_112308.1. NM_031046.3.
UniGeneiRn.89152.

3D structure databases

ProteinModelPortaliP29995.
SMRiP29995. Positions 7-225, 236-602.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi249575. 2 interactions.
STRINGi10116.ENSRNOP00000047905.

Protein family/group databases

TCDBi1.A.3.2.1. the ryanodine-inositol 1,4,5-triphosphate receptor ca(2+) channel (rir-cac) family.

PTM databases

iPTMnetiP29995.

Proteomic databases

PaxDbiP29995.
PRIDEiP29995.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi81678.
KEGGirno:81678.
UCSCiRGD:69649. rat.

Organism-specific databases

CTDi3709.
RGDi69649. Itpr2.

Phylogenomic databases

eggNOGiKOG3533. Eukaryota.
ENOG410XR97. LUCA.
HOGENOMiHOG000007660.
HOVERGENiHBG052158.
InParanoidiP29995.
KOiK04959.
PhylomeDBiP29995.

Enzyme and pathway databases

ReactomeiR-RNO-112043. PLC beta mediated events.
R-RNO-1489509. DAG and IP3 signaling.
R-RNO-2029485. Role of phospholipids in phagocytosis.
R-RNO-2871809. FCERI mediated Ca+2 mobilization.
R-RNO-4086398. Ca2+ pathway.
R-RNO-422356. Regulation of insulin secretion.
R-RNO-5218921. VEGFR2 mediated cell proliferation.
R-RNO-5578775. Ion homeostasis.
R-RNO-5607763. CLEC7A (Dectin-1) induces NFAT activation.
R-RNO-983695. Antigen activates B Cell Receptor (BCR) leading to generation of second messengers.

Miscellaneous databases

PROiP29995.

Family and domain databases

Gene3Di1.25.10.30. 2 hits.
InterProiIPR016024. ARM-type_fold.
IPR014821. Ins145_P3_rcpt.
IPR000493. InsP3_rcpt-bd.
IPR005821. Ion_trans_dom.
IPR016093. MIR_motif.
IPR013662. RIH_assoc-dom.
IPR000699. RIH_dom.
IPR015925. Ryanodine_recept-rel.
[Graphical view]
PANTHERiPTHR13715. PTHR13715. 1 hit.
PfamiPF08709. Ins145_P3_rec. 1 hit.
PF00520. Ion_trans. 1 hit.
PF02815. MIR. 1 hit.
PF08454. RIH_assoc. 1 hit.
PF01365. RYDR_ITPR. 2 hits.
[Graphical view]
PRINTSiPR00779. INSP3RECEPTR.
SMARTiSM00472. MIR. 4 hits.
[Graphical view]
SUPFAMiSSF100909. SSF100909. 2 hits.
SSF48371. SSF48371. 3 hits.
SSF82109. SSF82109. 2 hits.
PROSITEiPS50919. MIR. 5 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Structure of a novel InsP3 receptor."
    Suedhof T.C., Newton C.A., Archer B.T. III, Ushkaryov Y.A., Mignery G.A.
    EMBO J. 10:3199-3206(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS.
    Tissue: Brain.
  2. "New rat IP3R isoform 2 sequence."
    Magnino F., Dufour J.-F.
    Submitted (APR-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
  3. "Identification of a family of calcium sensors as protein ligands of inositol trisphosphate receptor Ca(2+) release channels."
    Yang J., McBride S., Mak D.-O.D., Vardi N., Palczewski K., Haeseleer F., Foskett J.K.
    Proc. Natl. Acad. Sci. U.S.A. 99:7711-7716(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CABP1.

Entry informationi

Entry nameiITPR2_RAT
AccessioniPrimary (citable) accession number: P29995
Secondary accession number(s): Q99P56
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: July 6, 2016
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.