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Protein

Phospho-2-dehydro-3-deoxyheptonate aldolase 1, chloroplastic

Gene

DHS1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Phosphoenolpyruvate + D-erythrose 4-phosphate + H2O = 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate.

Pathwayi: chorismate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate.
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. Phospho-2-dehydro-3-deoxyheptonate aldolase, Phospho-2-dehydro-3-deoxyheptonate aldolase 2, chloroplastic (DHS2), Phospho-2-dehydro-3-deoxyheptonate aldolase (At4g33510), Phospho-2-dehydro-3-deoxyheptonate aldolase (At4g33510), Phospho-2-dehydro-3-deoxyheptonate aldolase (F12K8.24), Phospho-2-dehydro-3-deoxyheptonate aldolase (AXX17_At1g23540), Phospho-2-dehydro-3-deoxyheptonate aldolase, Phospho-2-dehydro-3-deoxyheptonate aldolase (DHS2), Phospho-2-dehydro-3-deoxyheptonate aldolase (AXX17_At4g38330), Phospho-2-dehydro-3-deoxyheptonate aldolase (AXX17_At4g45420), Phospho-2-dehydro-3-deoxyheptonate aldolase 1, chloroplastic (DHS1)
  2. 3-dehydroquinate synthase, chloroplastic (DHQS)
  3. Bifunctional 3-dehydroquinate dehydratase/shikimate dehydrogenase, chloroplastic (EMB3004)
  4. Bifunctional 3-dehydroquinate dehydratase/shikimate dehydrogenase, chloroplastic (EMB3004)
  5. Shikimate kinase 2, chloroplastic (SK2), Shikimate kinase 1, chloroplastic (SK1)
  6. 3-phosphoshikimate 1-carboxyvinyltransferase (F27K7.11), 3-phosphoshikimate 1-carboxyvinyltransferase, 3-phosphoshikimate 1-carboxyvinyltransferase (AXX17_At2g42890), 3-phosphoshikimate 1-carboxyvinyltransferase (AXX17_At1g42980), 3-phosphoshikimate 1-carboxyvinyltransferase, chloroplastic (At2g45300)
  7. Chorismate synthase (At1g48850), Chorismate synthase, chloroplastic (EMB1144), Chorismate synthase (AXX17_At1g42970), Chorismate synthase (EMB1144), Chorismate synthase (EMB1144)
This subpathway is part of the pathway chorismate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate, the pathway chorismate biosynthesis and in Metabolic intermediate biosynthesis.

GO - Molecular functioni

  • 3-deoxy-7-phosphoheptulonate synthase activity Source: TAIR

GO - Biological processi

  • aromatic amino acid family biosynthetic process Source: TAIR
  • cellular amino acid biosynthetic process Source: UniProtKB-KW
  • chorismate biosynthetic process Source: TAIR
  • response to wounding Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis

Enzyme and pathway databases

BRENDAi2.5.1.54. 399.
UniPathwayiUPA00053; UER00084.

Names & Taxonomyi

Protein namesi
Recommended name:
Phospho-2-dehydro-3-deoxyheptonate aldolase 1, chloroplastic (EC:2.5.1.54)
Alternative name(s):
3-deoxy-D-arabino-heptulosonate 7-phosphate synthase 1
DAHP synthase 1
Phospho-2-keto-3-deoxyheptonate aldolase 1
Gene namesi
Name:DHS1
Ordered Locus Names:At4g39980
ORF Names:T5J17.150
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G39980.

Subcellular locationi

GO - Cellular componenti

  • chloroplast Source: TAIR
  • mitochondrion Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 52ChloroplastSequence analysisAdd BLAST52
ChainiPRO_000000229853 – 525Phospho-2-dehydro-3-deoxyheptonate aldolase 1, chloroplasticAdd BLAST473

Proteomic databases

PaxDbiP29976.
PRIDEiP29976.

Expressioni

Inductioni

By pathogen infection and wounding.

Gene expression databases

GenevisibleiP29976. AT.

Interactioni

Protein-protein interaction databases

BioGridi15439. 2 interactors.
IntActiP29976. 1 interactor.
STRINGi3702.AT4G39980.1.

Structurei

3D structure databases

ProteinModelPortaliP29976.
SMRiP29976.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class-II DAHP synthase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiENOG410IGEI. Eukaryota.
COG3200. LUCA.
HOGENOMiHOG000242917.
InParanoidiP29976.
KOiK01626.
OMAiDHPLMTT.
OrthoDBiEOG093607BP.
PhylomeDBiP29976.

Family and domain databases

InterProiIPR002480. DAHP_synth_2.
[Graphical view]
PfamiPF01474. DAHP_synth_2. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01358. DAHP_synth_II. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P29976-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALSNASSLS TRSIYGGDLS HRPSNRQSSF TFHPAVNTKP KSVNLVTAVH
60 70 80 90 100
AAEPARNAVS VKESVASSSS GALKWTPESW KLKKALQLPD YPNANELESV
110 120 130 140 150
LKTIEAFPPI VFAGEARNLE ERLADAAVGK AFLLQGGDCA ESFKEFNATN
160 170 180 190 200
IRDTFRVLLQ MSIVLTFGGQ VPVIKVGRMA GQFAKPRSDA FEEKDGVKLP
210 220 230 240 250
SYKGDNINGD TFDEKSRIPD PNRMIRAYTQ SAATLNLLRA FATGGYAAIQ
260 270 280 290 300
RVTQWNLDFV EQSEQADRYQ ELANRVDEAL GFMSACGLGT DHPLMTTTDF
310 320 330 340 350
YTSHECLLLP YEQSLTRLDS TSGLYYDCSA HMVWCGERTR QLDGAHVEFL
360 370 380 390 400
RGIANPLGIK VSNKMDPFEL VKLVEILNPN NKPGRITVIV RMGAENMRVK
410 420 430 440 450
LPHLIRAVRR SGQIVTWVCD PMHGNTIKAP CGLKTRAFDS ILAEVRAFLD
460 470 480 490 500
VHEQEGSHAG GIHLEMTGQN VTECIGGSRT VTYDDLSSRY HTHCDPRLNA
510 520
SQSLELAFIV AERLRKRRTG SQRVS
Length:525
Mass (Da):57,979
Last modified:January 23, 2002 - v2
Checksum:i13CA141A3C71D9E6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti429 – 430AP → ST in AAA32784 (PubMed:1681544).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M74819 mRNA. Translation: AAA32784.1.
AL035708 Genomic DNA. Translation: CAB38911.1.
AL161596 Genomic DNA. Translation: CAB80661.1.
CP002687 Genomic DNA. Translation: AEE87148.1.
AY090349 mRNA. Translation: AAL91255.1.
AY140052 mRNA. Translation: AAM98193.1.
BT000821 mRNA. Translation: AAN33196.1.
PIRiA41370.
T06104.
RefSeqiNP_195708.1. NM_120162.5.
UniGeneiAt.23161.

Genome annotation databases

EnsemblPlantsiAT4G39980.1; AT4G39980.1; AT4G39980.
GeneIDi830159.
GrameneiAT4G39980.1; AT4G39980.1; AT4G39980.
KEGGiath:AT4G39980.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M74819 mRNA. Translation: AAA32784.1.
AL035708 Genomic DNA. Translation: CAB38911.1.
AL161596 Genomic DNA. Translation: CAB80661.1.
CP002687 Genomic DNA. Translation: AEE87148.1.
AY090349 mRNA. Translation: AAL91255.1.
AY140052 mRNA. Translation: AAM98193.1.
BT000821 mRNA. Translation: AAN33196.1.
PIRiA41370.
T06104.
RefSeqiNP_195708.1. NM_120162.5.
UniGeneiAt.23161.

3D structure databases

ProteinModelPortaliP29976.
SMRiP29976.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi15439. 2 interactors.
IntActiP29976. 1 interactor.
STRINGi3702.AT4G39980.1.

Proteomic databases

PaxDbiP29976.
PRIDEiP29976.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G39980.1; AT4G39980.1; AT4G39980.
GeneIDi830159.
GrameneiAT4G39980.1; AT4G39980.1; AT4G39980.
KEGGiath:AT4G39980.

Organism-specific databases

TAIRiAT4G39980.

Phylogenomic databases

eggNOGiENOG410IGEI. Eukaryota.
COG3200. LUCA.
HOGENOMiHOG000242917.
InParanoidiP29976.
KOiK01626.
OMAiDHPLMTT.
OrthoDBiEOG093607BP.
PhylomeDBiP29976.

Enzyme and pathway databases

UniPathwayiUPA00053; UER00084.
BRENDAi2.5.1.54. 399.

Miscellaneous databases

PROiP29976.

Gene expression databases

GenevisibleiP29976. AT.

Family and domain databases

InterProiIPR002480. DAHP_synth_2.
[Graphical view]
PfamiPF01474. DAHP_synth_2. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01358. DAHP_synth_II. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiAROF_ARATH
AccessioniPrimary (citable) accession number: P29976
Secondary accession number(s): Q9SMQ7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: January 23, 2002
Last modified: November 30, 2016
This is version 130 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.