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Protein

Aquaporin-1

Gene

Aqp1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.

Miscellaneous

Pharmacologically inhibited by submillimolar concentrations of mercury.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei56Substrate discriminationBy similarity1
Sitei180Substrate discriminationBy similarity1
Sitei189Hg(2+)-sensitive residue1
Sitei195Substrate discriminationBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processTransport

Enzyme and pathway databases

ReactomeiR-RNO-1237044 Erythrocytes take up carbon dioxide and release oxygen
R-RNO-1247673 Erythrocytes take up oxygen and release carbon dioxide
R-RNO-432040 Vasopressin regulates renal water homeostasis via Aquaporins
R-RNO-432047 Passive transport by Aquaporins

Names & Taxonomyi

Protein namesi
Recommended name:
Aquaporin-1
Short name:
AQP-1
Alternative name(s):
Aquaporin-CHIP
Water channel protein for red blood cells and kidney proximal tubule
Gene namesi
Name:Aqp1
Synonyms:Chip28
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 4

Organism-specific databases

RGDi2141 Aqp1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 7CytoplasmicBy similarity7
Transmembranei8 – 36Helical; Name=Helix 1By similarityAdd BLAST29
Topological domaini37 – 48ExtracellularBy similarityAdd BLAST12
Transmembranei49 – 66Helical; Name=Helix 2By similarityAdd BLAST18
Topological domaini67 – 70CytoplasmicBy similarity4
Intramembranei71 – 76By similarity6
Intramembranei77 – 84Helical; Name=Helix BBy similarity8
Topological domaini85 – 94CytoplasmicBy similarity10
Transmembranei95 – 115Helical; Name=Helix 3By similarityAdd BLAST21
Topological domaini116 – 136ExtracellularBy similarityAdd BLAST21
Transmembranei137 – 155Helical; Name=Helix 4By similarityAdd BLAST19
Topological domaini156 – 166CytoplasmicBy similarityAdd BLAST11
Transmembranei167 – 183Helical; Name=Helix 5By similarityAdd BLAST17
Topological domaini184 – 186ExtracellularBy similarity3
Intramembranei187 – 192By similarity6
Intramembranei193 – 200Helical; Name=Helix EBy similarity8
Topological domaini201 – 207ExtracellularBy similarity7
Transmembranei208 – 228Helical; Name=Helix 6By similarityAdd BLAST21
Topological domaini229 – 269CytoplasmicBy similarityAdd BLAST41

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000639241 – 269Aquaporin-1Add BLAST269

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi42N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi205N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei247PhosphoserineBy similarity1
Modified residuei253PhosphotyrosineBy similarity1
Modified residuei262PhosphoserineCombined sources1

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP29975
PRIDEiP29975

PTM databases

iPTMnetiP29975
PhosphoSitePlusiP29975
SwissPalmiP29975

Expressioni

Tissue specificityi

Erythrocytes and renal tubules.

Gene expression databases

BgeeiENSRNOG00000011648
ExpressionAtlasiP29975 baseline and differential
GenevisibleiP29975 RN

Interactioni

Subunit structurei

Homotetramer (By similarity). Interacts with EPHB2; involved in endolymph production in the inner ear. Identified in a complex with STOM (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

CORUMiP29975
STRINGi10116.ENSRNOP00000015692

Structurei

3D structure databases

ProteinModelPortaliP29975
SMRiP29975
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi76 – 78NPA 13
Motifi192 – 194NPA 23

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi159 – 162Poly-Arg4

Domaini

Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).

Sequence similaritiesi

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0223 Eukaryota
COG0580 LUCA
GeneTreeiENSGT00760000119223
HOGENOMiHOG000288286
HOVERGENiHBG000312
InParanoidiP29975
KOiK09864
OMAiELFGTFW
OrthoDBiEOG091G166T
PhylomeDBiP29975
TreeFamiTF312940

Family and domain databases

CDDicd00333 MIP, 1 hit
Gene3Di1.20.1080.10, 1 hit
InterProiView protein in InterPro
IPR023271 Aquaporin-like
IPR023274 Aquaporin_1
IPR034294 Aquaporin_transptr
IPR000425 MIP
IPR022357 MIP_CS
PANTHERiPTHR19139 PTHR19139, 1 hit
PfamiView protein in Pfam
PF00230 MIP, 1 hit
PRINTSiPR02013 AQUAPORIN1
PR00783 MINTRINSICP
SUPFAMiSSF81338 SSF81338, 1 hit
TIGRFAMsiTIGR00861 MIP, 1 hit
PROSITEiView protein in PROSITE
PS00221 MIP, 1 hit

Sequencei

Sequence statusi: Complete.

P29975-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASEIKKKLF WRAVVAEFLA MTLFVFISIG SALGFNYPLE RNQTLVQDNV
60 70 80 90 100
KVSLAFGLSI ATLAQSVGHI SGAHLNPAVT LGLLLSCQIS ILRAVMYIIA
110 120 130 140 150
QCVGAIVASA ILSGITSSLL ENSLGRNDLA RGVNSGQGLG IEIIGTLQLV
160 170 180 190 200
LCVLATTDRR RRDLGGSAPL AIGLSVALGH LLAIDYTGCG INPARSFGSA
210 220 230 240 250
VLTRNFSNHW IFWVGPFIGS ALAVLIYDFI LAPRSSDFTD RMKVWTSGQV
260
EEYDLDADDI NSRVEMKPK
Length:269
Mass (Da):28,857
Last modified:January 23, 2007 - v4
Checksum:i69BE16F65212AAB6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti3S → T in CAA50395 (PubMed:8508530).Curated1
Sequence conflicti5I → F in CAA49761 (PubMed:8421053).Curated1
Sequence conflicti75L → S in AAB46624 (PubMed:7530838).Curated1
Sequence conflicti159R → Q in CAA50395 (PubMed:8508530).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X67948 mRNA Translation: CAA48134.1
L07268 mRNA Translation: AAB46624.1
X70257 mRNA Translation: CAA49761.1
X71069 mRNA Translation: CAA50395.1
BC090068 mRNA Translation: AAH90068.1
PIRiJC1320
RefSeqiNP_036910.1, NM_012778.1
UniGeneiRn.1618

Genome annotation databases

EnsembliENSRNOT00000015692; ENSRNOP00000015692; ENSRNOG00000011648
GeneIDi25240
KEGGirno:25240
UCSCiRGD:2141 rat

Similar proteinsi

Entry informationi

Entry nameiAQP1_RAT
AccessioniPrimary (citable) accession number: P29975
Secondary accession number(s): Q5EB50, Q7TN36
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: January 23, 2007
Last modified: May 23, 2018
This is version 169 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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