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Protein

Aquaporin-1

Gene

Aqp1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei56 – 561Substrate discriminationBy similarity
Sitei180 – 1801Substrate discriminationBy similarity
Sitei189 – 1891Hg(2+)-sensitive residue
Sitei195 – 1951Substrate discriminationBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

ReactomeiR-RNO-1237044. Erythrocytes take up carbon dioxide and release oxygen.
R-RNO-1247673. Erythrocytes take up oxygen and release carbon dioxide.
R-RNO-432040. Vasopressin regulates renal water homeostasis via Aquaporins.
R-RNO-432047. Passive transport by Aquaporins.

Names & Taxonomyi

Protein namesi
Recommended name:
Aquaporin-1
Short name:
AQP-1
Alternative name(s):
Aquaporin-CHIP
Water channel protein for red blood cells and kidney proximal tubule
Gene namesi
Name:Aqp1
Synonyms:Chip28
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 4

Organism-specific databases

RGDi2141. Aqp1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini2 – 76CytoplasmicBy similarity
Transmembranei8 – 3629Helical; Name=Helix 1By similarityAdd
BLAST
Topological domaini37 – 4812ExtracellularBy similarityAdd
BLAST
Transmembranei49 – 6618Helical; Name=Helix 2By similarityAdd
BLAST
Topological domaini67 – 704CytoplasmicBy similarity
Intramembranei71 – 766By similarity
Intramembranei77 – 848Helical; Name=Helix BBy similarity
Topological domaini85 – 9410CytoplasmicBy similarity
Transmembranei95 – 11521Helical; Name=Helix 3By similarityAdd
BLAST
Topological domaini116 – 13621ExtracellularBy similarityAdd
BLAST
Transmembranei137 – 15519Helical; Name=Helix 4By similarityAdd
BLAST
Topological domaini156 – 16611CytoplasmicBy similarityAdd
BLAST
Transmembranei167 – 18317Helical; Name=Helix 5By similarityAdd
BLAST
Topological domaini184 – 1863ExtracellularBy similarity
Intramembranei187 – 1926By similarity
Intramembranei193 – 2008Helical; Name=Helix EBy similarity
Topological domaini201 – 2077ExtracellularBy similarity
Transmembranei208 – 22821Helical; Name=Helix 6By similarityAdd
BLAST
Topological domaini229 – 26941CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

  • apical plasma membrane Source: UniProtKB
  • axon terminus Source: RGD
  • basal plasma membrane Source: UniProtKB
  • basolateral plasma membrane Source: UniProtKB
  • brush border Source: UniProtKB
  • brush border membrane Source: UniProtKB
  • cytoplasm Source: UniProtKB
  • extracellular exosome Source: Ensembl
  • integral component of membrane Source: UniProtKB-KW
  • neuronal cell body membrane Source: RGD
  • neuron projection Source: RGD
  • nuclear membrane Source: UniProtKB
  • nucleus Source: UniProtKB
  • plasma membrane Source: UniProtKB
  • sarcolemma Source: UniProtKB
  • symbiont-containing vacuole Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 269268Aquaporin-1PRO_0000063924Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi42 – 421N-linked (GlcNAc...)Sequence analysis
Glycosylationi205 – 2051N-linked (GlcNAc...)Sequence analysis
Modified residuei247 – 2471PhosphoserineBy similarity
Modified residuei253 – 2531PhosphotyrosineBy similarity
Modified residuei262 – 2621PhosphoserineCombined sources

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP29975.
PRIDEiP29975.

PTM databases

SwissPalmiP29975.

Expressioni

Tissue specificityi

Erythrocytes and renal tubules.

Gene expression databases

ExpressionAtlasiP29975. baseline and differential.
GenevisibleiP29975. RN.

Interactioni

Subunit structurei

Homotetramer (By similarity). Interacts with EPHB2; involved in endolymph production in the inner ear. Identified in a complex with STOM (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000015692.

Structurei

3D structure databases

ProteinModelPortaliP29975.
SMRiP29975. Positions 1-247.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi76 – 783NPA 1
Motifi192 – 1943NPA 2

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi159 – 1624Poly-Arg

Domaini

Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).

Sequence similaritiesi

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0223. Eukaryota.
COG0580. LUCA.
GeneTreeiENSGT00760000119223.
HOGENOMiHOG000288286.
HOVERGENiHBG000312.
InParanoidiP29975.
KOiK09864.
OMAiAFCARVM.
OrthoDBiEOG7N8ZWD.
PhylomeDBiP29975.
TreeFamiTF312940.

Family and domain databases

Gene3Di1.20.1080.10. 1 hit.
InterProiIPR023271. Aquaporin-like.
IPR023274. Aquaporin_1.
IPR000425. MIP.
IPR022357. MIP_CS.
[Graphical view]
PANTHERiPTHR19139. PTHR19139. 1 hit.
PfamiPF00230. MIP. 1 hit.
[Graphical view]
PRINTSiPR02013. AQUAPORIN1.
PR00783. MINTRINSICP.
SUPFAMiSSF81338. SSF81338. 1 hit.
TIGRFAMsiTIGR00861. MIP. 1 hit.
PROSITEiPS00221. MIP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P29975-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASEIKKKLF WRAVVAEFLA MTLFVFISIG SALGFNYPLE RNQTLVQDNV
60 70 80 90 100
KVSLAFGLSI ATLAQSVGHI SGAHLNPAVT LGLLLSCQIS ILRAVMYIIA
110 120 130 140 150
QCVGAIVASA ILSGITSSLL ENSLGRNDLA RGVNSGQGLG IEIIGTLQLV
160 170 180 190 200
LCVLATTDRR RRDLGGSAPL AIGLSVALGH LLAIDYTGCG INPARSFGSA
210 220 230 240 250
VLTRNFSNHW IFWVGPFIGS ALAVLIYDFI LAPRSSDFTD RMKVWTSGQV
260
EEYDLDADDI NSRVEMKPK
Length:269
Mass (Da):28,857
Last modified:January 23, 2007 - v4
Checksum:i69BE16F65212AAB6
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti3 – 31S → T in CAA50395 (PubMed:8508530).Curated
Sequence conflicti5 – 51I → F in CAA49761 (PubMed:8421053).Curated
Sequence conflicti75 – 751L → S in AAB46624 (PubMed:7530838).Curated
Sequence conflicti159 – 1591R → Q in CAA50395 (PubMed:8508530).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X67948 mRNA. Translation: CAA48134.1.
L07268 mRNA. Translation: AAB46624.1.
X70257 mRNA. Translation: CAA49761.1.
X71069 mRNA. Translation: CAA50395.1.
BC090068 mRNA. Translation: AAH90068.1.
PIRiJC1320.
RefSeqiNP_036910.1. NM_012778.1.
UniGeneiRn.1618.

Genome annotation databases

EnsembliENSRNOT00000015692; ENSRNOP00000015692; ENSRNOG00000011648.
GeneIDi25240.
KEGGirno:25240.
UCSCiRGD:2141. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X67948 mRNA. Translation: CAA48134.1.
L07268 mRNA. Translation: AAB46624.1.
X70257 mRNA. Translation: CAA49761.1.
X71069 mRNA. Translation: CAA50395.1.
BC090068 mRNA. Translation: AAH90068.1.
PIRiJC1320.
RefSeqiNP_036910.1. NM_012778.1.
UniGeneiRn.1618.

3D structure databases

ProteinModelPortaliP29975.
SMRiP29975. Positions 1-247.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000015692.

PTM databases

SwissPalmiP29975.

Proteomic databases

PaxDbiP29975.
PRIDEiP29975.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000015692; ENSRNOP00000015692; ENSRNOG00000011648.
GeneIDi25240.
KEGGirno:25240.
UCSCiRGD:2141. rat.

Organism-specific databases

CTDi358.
RGDi2141. Aqp1.

Phylogenomic databases

eggNOGiKOG0223. Eukaryota.
COG0580. LUCA.
GeneTreeiENSGT00760000119223.
HOGENOMiHOG000288286.
HOVERGENiHBG000312.
InParanoidiP29975.
KOiK09864.
OMAiAFCARVM.
OrthoDBiEOG7N8ZWD.
PhylomeDBiP29975.
TreeFamiTF312940.

Enzyme and pathway databases

ReactomeiR-RNO-1237044. Erythrocytes take up carbon dioxide and release oxygen.
R-RNO-1247673. Erythrocytes take up oxygen and release carbon dioxide.
R-RNO-432040. Vasopressin regulates renal water homeostasis via Aquaporins.
R-RNO-432047. Passive transport by Aquaporins.

Miscellaneous databases

NextBioi605823.
PROiP29975.

Gene expression databases

ExpressionAtlasiP29975. baseline and differential.
GenevisibleiP29975. RN.

Family and domain databases

Gene3Di1.20.1080.10. 1 hit.
InterProiIPR023271. Aquaporin-like.
IPR023274. Aquaporin_1.
IPR000425. MIP.
IPR022357. MIP_CS.
[Graphical view]
PANTHERiPTHR19139. PTHR19139. 1 hit.
PfamiPF00230. MIP. 1 hit.
[Graphical view]
PRINTSiPR02013. AQUAPORIN1.
PR00783. MINTRINSICP.
SUPFAMiSSF81338. SSF81338. 1 hit.
TIGRFAMsiTIGR00861. MIP. 1 hit.
PROSITEiPS00221. MIP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation of a cDNA for rat CHIP28 water channel: high mRNA expression in kidney cortex and inner medulla."
    Deen P.M.T., Dempster J.A., Wieringa B., van Os C.H.
    Biochem. Biophys. Res. Commun. 188:1267-1273(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Kidney.
  2. "Examination of rat salivary glands for the presence of the aquaporin CHIP."
    Li J., Nielsen S., Dai Y., Lazowski K.W., Christensen E.I., Tabak L.A., Baum B.J.
    Pflugers Arch. 428:455-460(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Wistar.
    Tissue: Parotid gland.
  3. "Cloning, functional analysis and cell localization of a kidney proximal tubule water transporter homologous to CHIP28."
    Zhang R., Skach W., Hasegawa H., van Hoek A.N., Verkman A.S.
    J. Cell Biol. 120:359-369(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Kidney.
  4. "Isolation of gene markers of differentiated and proliferating vascular smooth muscle cells."
    Shanahan C.M., Weissberg P.L., Metcalfe J.C.
    Circ. Res. 73:193-204(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Wistar.
    Tissue: Aortic smooth muscle.
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Heart.
  6. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-262, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiAQP1_RAT
AccessioniPrimary (citable) accession number: P29975
Secondary accession number(s): Q5EB50, Q7TN36
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: January 23, 2007
Last modified: May 11, 2016
This is version 149 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Pharmacologically inhibited by submillimolar concentrations of mercury.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.