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P29974

- CNGA1_MOUSE

UniProt

P29974 - CNGA1_MOUSE

Protein

cGMP-gated cation channel alpha-1

Gene

Cnga1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 128 (01 Oct 2014)
      Sequence version 2 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Visual signal transduction is mediated by a G-protein coupled cascade using cGMP as second messenger. This protein can be activated by cyclic GMP which leads to an opening of the cation channel and thereby causing a depolarization of rod photoreceptors.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei538 – 5381cGMPSequence Analysis
    Binding sitei553 – 5531cGMPSequence Analysis

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi479 – 601123cGMPSequence AnalysisAdd
    BLAST

    GO - Molecular functioni

    1. cGMP binding Source: RefGenome
    2. intracellular cAMP activated cation channel activity Source: RefGenome
    3. intracellular cGMP activated cation channel activity Source: RefGenome
    4. voltage-gated potassium channel activity Source: RefGenome

    GO - Biological processi

    1. phototransduction, visible light Source: RefGenome
    2. potassium ion transmembrane transport Source: RefGenome
    3. regulation of membrane potential Source: RefGenome
    4. visual perception Source: UniProtKB-KW

    Keywords - Molecular functioni

    Ion channel, Ligand-gated ion channel

    Keywords - Biological processi

    Ion transport, Sensory transduction, Transport, Vision

    Keywords - Ligandi

    cGMP, cGMP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_188252. Activation of the phototransduction cascade.
    REACT_188266. Inactivation, recovery and regulation of the phototransduction cascade.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    cGMP-gated cation channel alpha-1
    Alternative name(s):
    Cyclic nucleotide-gated cation channel 1
    Cyclic nucleotide-gated channel alpha-1
    Short name:
    CNG channel alpha-1
    Short name:
    CNG-1
    Short name:
    CNG1
    Cyclic nucleotide-gated channel, photoreceptor
    Rod photoreceptor cGMP-gated channel subunit alpha
    Gene namesi
    Name:Cnga1
    Synonyms:Cncg, Cncg1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 5

    Organism-specific databases

    MGIiMGI:88436. Cnga1.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of plasma membrane Source: RefGenome
    2. membrane Source: MGI
    3. photoreceptor outer segment Source: MGI
    4. photoreceptor outer segment membrane Source: BHF-UCL

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 684684cGMP-gated cation channel alpha-1PRO_0000219309Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi417 – 4171N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PRIDEiP29974.

    PTM databases

    PhosphoSiteiP29974.

    Expressioni

    Tissue specificityi

    Rod cells in the retina and inner medulla of kidney.

    Gene expression databases

    BgeeiP29974.
    CleanExiMM_CNGA1.
    GenevestigatoriP29974.

    Interactioni

    Subunit structurei

    Tetramer formed of three CNGA1 and one CNGB1 modulatory subunits.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP29974.
    SMRiP29974. Positions 340-588, 615-664.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 156156CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini178 – 19013ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini210 – 23324CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini254 – 29138ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini315 – 36652CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini387 – 47084ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini492 – 684193CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei157 – 17721Helical; Name=H1Sequence AnalysisAdd
    BLAST
    Transmembranei191 – 20919Helical; Name=H2Sequence AnalysisAdd
    BLAST
    Transmembranei234 – 25320Helical; Name=H3Sequence AnalysisAdd
    BLAST
    Transmembranei292 – 31423Helical; Name=H4Sequence AnalysisAdd
    BLAST
    Transmembranei367 – 38620Helical; Name=H5Sequence AnalysisAdd
    BLAST
    Transmembranei471 – 49121Helical; Name=H6Sequence AnalysisAdd
    BLAST

    Family & Domainsi

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili615 – 65844By similarityAdd
    BLAST

    Domaini

    The C-terminal coiled-coil domain mediates homotrimerization of CNGA subunits.By similarity

    Sequence similaritiesi

    Contains 1 cyclic nucleotide-binding domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG300025.
    GeneTreeiENSGT00550000074376.
    HOGENOMiHOG000007898.
    HOVERGENiHBG000281.
    InParanoidiP29974.
    KOiK04948.
    OMAiWTMVIAR.
    OrthoDBiEOG771268.
    PhylomeDBiP29974.
    TreeFamiTF319048.

    Family and domain databases

    Gene3Di2.60.120.10. 1 hit.
    InterProiIPR018490. cNMP-bd-like.
    IPR018488. cNMP-bd_CS.
    IPR000595. cNMP-bd_dom.
    IPR005821. Ion_trans_dom.
    IPR014710. RmlC-like_jellyroll.
    [Graphical view]
    PfamiPF00027. cNMP_binding. 1 hit.
    PF00520. Ion_trans. 1 hit.
    [Graphical view]
    SMARTiSM00100. cNMP. 1 hit.
    [Graphical view]
    SUPFAMiSSF51206. SSF51206. 1 hit.
    PROSITEiPS00888. CNMP_BINDING_1. 1 hit.
    PS00889. CNMP_BINDING_2. 1 hit.
    PS50042. CNMP_BINDING_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P29974-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKTNIINTWH SFVNIPNVIV PAIEKEIRRM ENGACSSFSD DDNGSLSEES    50
    ENEDSFFRSN SYKRRGPSQR EQHLPGTMAL FNVNNSSNKD QEPKEKKKKK 100
    KEKKSKADDK NENKKDPEKK KKKEKEKEKK KKEEKTKEKK EEEKKEVVVI 150
    DPSGNTYYNW LFCITLPVMY NWTMIIARAC FDELQSDYLE YWLIFDYVSD 200
    VVYLADMFVR TRTGYLEQGL LVKDRMKLIE KYKANLQFKL DVLSVIPTDL 250
    LYIKFGWNYP EIRLNRLLRI SRMFEFFQRT ETRTNYPNIF RISNLVMYIV 300
    IIIHWNACVY YSISKAIGFG NDTWVYPDVN DPEFGRLARK YVYSLYWSTL 350
    TLTTIGETPP PVLDSEYIFV VVDFLIGVLI FATIVGNIGS MISNMNAARA 400
    EFQSRVDAIK QYMNFRNVSK DMEKRVIKWF DYLWTNKKTV DEREVLRYLP 450
    DKLRAEIAIN VHLDTLKKVR IFADCEAGLL VELVLKLQPQ VYSPGDYICK 500
    KGDIGREMYI IKEGKLAVVA DDGITQFVVL SDGSYFGEIS ILNIKGSKAG 550
    NRRTANIKSI GYSDLFCLSK DDLMEALTEY PDAKTMLEEK GRQILMKDGL 600
    LDINIANMGS DPKDLEEKVT RMEGSVDLLQ TRFARILAEY ESMQQKLKQR 650
    LTKVEKFLKP LIETEFSALE EPGGESELTE SLQD 684
    Length:684
    Mass (Da):79,460
    Last modified:November 1, 1997 - v2
    Checksum:i13EC2A405B6B8CBF
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti113 – 1142NK → I in AAA37425. (PubMed:1372902)Curated
    Sequence conflicti200 – 2001D → N in AAA37425. (PubMed:1372902)Curated
    Sequence conflicti576 – 5761A → V in AAA37425. (PubMed:1372902)Curated
    Sequence conflicti635 – 6351R → C in AAA37425. (PubMed:1372902)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M84742 mRNA. Translation: AAA37425.1.
    U19717 mRNA. Translation: AAA85702.1.
    U19715 mRNA. Translation: AAA85700.1.
    U19716 mRNA. Translation: AAA85701.1.
    BC141261 mRNA. Translation: AAI41262.1.
    CCDSiCCDS19333.1.
    RefSeqiNP_031749.2. NM_007723.2.
    UniGeneiMm.436652.

    Genome annotation databases

    EnsembliENSMUST00000087213; ENSMUSP00000084464; ENSMUSG00000067220.
    ENSMUST00000135701; ENSMUSP00000119886; ENSMUSG00000067220.
    ENSMUST00000169997; ENSMUSP00000132329; ENSMUSG00000067220.
    GeneIDi12788.
    KEGGimmu:12788.
    UCSCiuc008xrr.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M84742 mRNA. Translation: AAA37425.1 .
    U19717 mRNA. Translation: AAA85702.1 .
    U19715 mRNA. Translation: AAA85700.1 .
    U19716 mRNA. Translation: AAA85701.1 .
    BC141261 mRNA. Translation: AAI41262.1 .
    CCDSi CCDS19333.1.
    RefSeqi NP_031749.2. NM_007723.2.
    UniGenei Mm.436652.

    3D structure databases

    ProteinModelPortali P29974.
    SMRi P29974. Positions 340-588, 615-664.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei P29974.

    Proteomic databases

    PRIDEi P29974.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000087213 ; ENSMUSP00000084464 ; ENSMUSG00000067220 .
    ENSMUST00000135701 ; ENSMUSP00000119886 ; ENSMUSG00000067220 .
    ENSMUST00000169997 ; ENSMUSP00000132329 ; ENSMUSG00000067220 .
    GeneIDi 12788.
    KEGGi mmu:12788.
    UCSCi uc008xrr.1. mouse.

    Organism-specific databases

    CTDi 1259.
    MGIi MGI:88436. Cnga1.

    Phylogenomic databases

    eggNOGi NOG300025.
    GeneTreei ENSGT00550000074376.
    HOGENOMi HOG000007898.
    HOVERGENi HBG000281.
    InParanoidi P29974.
    KOi K04948.
    OMAi WTMVIAR.
    OrthoDBi EOG771268.
    PhylomeDBi P29974.
    TreeFami TF319048.

    Enzyme and pathway databases

    Reactomei REACT_188252. Activation of the phototransduction cascade.
    REACT_188266. Inactivation, recovery and regulation of the phototransduction cascade.

    Miscellaneous databases

    NextBioi 282186.
    PROi P29974.
    SOURCEi Search...

    Gene expression databases

    Bgeei P29974.
    CleanExi MM_CNGA1.
    Genevestigatori P29974.

    Family and domain databases

    Gene3Di 2.60.120.10. 1 hit.
    InterProi IPR018490. cNMP-bd-like.
    IPR018488. cNMP-bd_CS.
    IPR000595. cNMP-bd_dom.
    IPR005821. Ion_trans_dom.
    IPR014710. RmlC-like_jellyroll.
    [Graphical view ]
    Pfami PF00027. cNMP_binding. 1 hit.
    PF00520. Ion_trans. 1 hit.
    [Graphical view ]
    SMARTi SM00100. cNMP. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51206. SSF51206. 1 hit.
    PROSITEi PS00888. CNMP_BINDING_1. 1 hit.
    PS00889. CNMP_BINDING_2. 1 hit.
    PS50042. CNMP_BINDING_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Primary structure and chromosomal localization of human and mouse rod photoreceptor cGMP-gated cation channel."
      Pittler S.J., Lee A.K., Altherr M.R., Howard T.A., Seldin M.F., Hurwitz R.L., Wasmuth J.J., Baehr W.
      J. Biol. Chem. 267:6257-6262(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Retina.
    2. "Cloning of a cGMP-gated cation channel from mouse kidney inner medullary collecting duct."
      Karlson K.H., Ciampolillo-Bates F., McCoy D.E., Kizer N.L., Stanton B.A.
      Biochim. Biophys. Acta 1236:197-200(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Kidney.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain.

    Entry informationi

    Entry nameiCNGA1_MOUSE
    AccessioniPrimary (citable) accession number: P29974
    Secondary accession number(s): B9EJ09, Q60776
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1993
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 128 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3