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Protein

cGMP-gated cation channel alpha-1

Gene

Cnga1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Visual signal transduction is mediated by a G-protein coupled cascade using cGMP as second messenger. This protein can be activated by cyclic GMP which leads to an opening of the cation channel and thereby causing a depolarization of rod photoreceptors.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei538cGMPSequence analysis1
Binding sitei553cGMPSequence analysis1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi479 – 601cGMPSequence analysisAdd BLAST123

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Ligand-gated ion channel

Keywords - Biological processi

Ion transport, Sensory transduction, Transport, Vision

Keywords - Ligandi

cGMP, cGMP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-2485179. Activation of the phototransduction cascade.
R-MMU-2514859. Inactivation, recovery and regulation of the phototransduction cascade.

Names & Taxonomyi

Protein namesi
Recommended name:
cGMP-gated cation channel alpha-1
Alternative name(s):
Cyclic nucleotide-gated cation channel 1
Cyclic nucleotide-gated channel alpha-1
Short name:
CNG channel alpha-1
Short name:
CNG-1
Short name:
CNG1
Cyclic nucleotide-gated channel, photoreceptor
Rod photoreceptor cGMP-gated channel subunit alpha
Gene namesi
Name:Cnga1
Synonyms:Cncg, Cncg1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:88436. Cnga1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 156CytoplasmicSequence analysisAdd BLAST156
Transmembranei157 – 177Helical; Name=H1Sequence analysisAdd BLAST21
Topological domaini178 – 190ExtracellularSequence analysisAdd BLAST13
Transmembranei191 – 209Helical; Name=H2Sequence analysisAdd BLAST19
Topological domaini210 – 233CytoplasmicSequence analysisAdd BLAST24
Transmembranei234 – 253Helical; Name=H3Sequence analysisAdd BLAST20
Topological domaini254 – 291ExtracellularSequence analysisAdd BLAST38
Transmembranei292 – 314Helical; Name=H4Sequence analysisAdd BLAST23
Topological domaini315 – 366CytoplasmicSequence analysisAdd BLAST52
Transmembranei367 – 386Helical; Name=H5Sequence analysisAdd BLAST20
Topological domaini387 – 470ExtracellularSequence analysisAdd BLAST84
Transmembranei471 – 491Helical; Name=H6Sequence analysisAdd BLAST21
Topological domaini492 – 684CytoplasmicSequence analysisAdd BLAST193

GO - Cellular componenti

  • integral component of plasma membrane Source: GO_Central
  • membrane Source: MGI
  • photoreceptor outer segment Source: MGI
  • photoreceptor outer segment membrane Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002193091 – 684cGMP-gated cation channel alpha-1Add BLAST684

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi417N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP29974.
PaxDbiP29974.
PRIDEiP29974.

PTM databases

iPTMnetiP29974.
PhosphoSitePlusiP29974.

Expressioni

Tissue specificityi

Rod cells in the retina and inner medulla of kidney.

Gene expression databases

BgeeiENSMUSG00000067220.
CleanExiMM_CNGA1.
ExpressionAtlasiP29974. baseline and differential.
GenevisibleiP29974. MM.

Interactioni

Subunit structurei

Tetramer formed of three CNGA1 and one CNGB1 modulatory subunits.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000084464.

Structurei

3D structure databases

ProteinModelPortaliP29974.
SMRiP29974.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili615 – 658By similarityAdd BLAST44

Domaini

The C-terminal coiled-coil domain mediates homotrimerization of CNGA subunits.By similarity

Sequence similaritiesi

Contains 1 cyclic nucleotide-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0500. Eukaryota.
ENOG410YWWI. LUCA.
GeneTreeiENSGT00760000118772.
HOGENOMiHOG000007898.
HOVERGENiHBG000281.
InParanoidiP29974.
KOiK04948.
OMAiYLEYWLI.
OrthoDBiEOG091G03EW.
PhylomeDBiP29974.
TreeFamiTF319048.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
InterProiIPR032406. CLZ_dom.
IPR032945. CNGA1.
IPR018490. cNMP-bd-like.
IPR018488. cNMP-bd_CS.
IPR000595. cNMP-bd_dom.
IPR005821. Ion_trans_dom.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PANTHERiPTHR10217:SF387. PTHR10217:SF387. 1 hit.
PfamiPF16526. CLZ. 1 hit.
PF00027. cNMP_binding. 1 hit.
PF00520. Ion_trans. 1 hit.
[Graphical view]
SMARTiSM00100. cNMP. 1 hit.
[Graphical view]
SUPFAMiSSF51206. SSF51206. 1 hit.
PROSITEiPS00888. CNMP_BINDING_1. 1 hit.
PS00889. CNMP_BINDING_2. 1 hit.
PS50042. CNMP_BINDING_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P29974-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTNIINTWH SFVNIPNVIV PAIEKEIRRM ENGACSSFSD DDNGSLSEES
60 70 80 90 100
ENEDSFFRSN SYKRRGPSQR EQHLPGTMAL FNVNNSSNKD QEPKEKKKKK
110 120 130 140 150
KEKKSKADDK NENKKDPEKK KKKEKEKEKK KKEEKTKEKK EEEKKEVVVI
160 170 180 190 200
DPSGNTYYNW LFCITLPVMY NWTMIIARAC FDELQSDYLE YWLIFDYVSD
210 220 230 240 250
VVYLADMFVR TRTGYLEQGL LVKDRMKLIE KYKANLQFKL DVLSVIPTDL
260 270 280 290 300
LYIKFGWNYP EIRLNRLLRI SRMFEFFQRT ETRTNYPNIF RISNLVMYIV
310 320 330 340 350
IIIHWNACVY YSISKAIGFG NDTWVYPDVN DPEFGRLARK YVYSLYWSTL
360 370 380 390 400
TLTTIGETPP PVLDSEYIFV VVDFLIGVLI FATIVGNIGS MISNMNAARA
410 420 430 440 450
EFQSRVDAIK QYMNFRNVSK DMEKRVIKWF DYLWTNKKTV DEREVLRYLP
460 470 480 490 500
DKLRAEIAIN VHLDTLKKVR IFADCEAGLL VELVLKLQPQ VYSPGDYICK
510 520 530 540 550
KGDIGREMYI IKEGKLAVVA DDGITQFVVL SDGSYFGEIS ILNIKGSKAG
560 570 580 590 600
NRRTANIKSI GYSDLFCLSK DDLMEALTEY PDAKTMLEEK GRQILMKDGL
610 620 630 640 650
LDINIANMGS DPKDLEEKVT RMEGSVDLLQ TRFARILAEY ESMQQKLKQR
660 670 680
LTKVEKFLKP LIETEFSALE EPGGESELTE SLQD
Length:684
Mass (Da):79,460
Last modified:November 1, 1997 - v2
Checksum:i13EC2A405B6B8CBF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti113 – 114NK → I in AAA37425 (PubMed:1372902).Curated2
Sequence conflicti200D → N in AAA37425 (PubMed:1372902).Curated1
Sequence conflicti576A → V in AAA37425 (PubMed:1372902).Curated1
Sequence conflicti635R → C in AAA37425 (PubMed:1372902).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M84742 mRNA. Translation: AAA37425.1.
U19717 mRNA. Translation: AAA85702.1.
U19715 mRNA. Translation: AAA85700.1.
U19716 mRNA. Translation: AAA85701.1.
BC141261 mRNA. Translation: AAI41262.1.
CCDSiCCDS19333.1.
RefSeqiNP_031749.2. NM_007723.2.
UniGeneiMm.436652.

Genome annotation databases

EnsembliENSMUST00000087213; ENSMUSP00000084464; ENSMUSG00000067220.
ENSMUST00000169997; ENSMUSP00000132329; ENSMUSG00000067220.
ENSMUST00000201463; ENSMUSP00000143881; ENSMUSG00000067220.
GeneIDi12788.
KEGGimmu:12788.
UCSCiuc008xrr.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M84742 mRNA. Translation: AAA37425.1.
U19717 mRNA. Translation: AAA85702.1.
U19715 mRNA. Translation: AAA85700.1.
U19716 mRNA. Translation: AAA85701.1.
BC141261 mRNA. Translation: AAI41262.1.
CCDSiCCDS19333.1.
RefSeqiNP_031749.2. NM_007723.2.
UniGeneiMm.436652.

3D structure databases

ProteinModelPortaliP29974.
SMRiP29974.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000084464.

PTM databases

iPTMnetiP29974.
PhosphoSitePlusiP29974.

Proteomic databases

MaxQBiP29974.
PaxDbiP29974.
PRIDEiP29974.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000087213; ENSMUSP00000084464; ENSMUSG00000067220.
ENSMUST00000169997; ENSMUSP00000132329; ENSMUSG00000067220.
ENSMUST00000201463; ENSMUSP00000143881; ENSMUSG00000067220.
GeneIDi12788.
KEGGimmu:12788.
UCSCiuc008xrr.1. mouse.

Organism-specific databases

CTDi1259.
MGIiMGI:88436. Cnga1.

Phylogenomic databases

eggNOGiKOG0500. Eukaryota.
ENOG410YWWI. LUCA.
GeneTreeiENSGT00760000118772.
HOGENOMiHOG000007898.
HOVERGENiHBG000281.
InParanoidiP29974.
KOiK04948.
OMAiYLEYWLI.
OrthoDBiEOG091G03EW.
PhylomeDBiP29974.
TreeFamiTF319048.

Enzyme and pathway databases

ReactomeiR-MMU-2485179. Activation of the phototransduction cascade.
R-MMU-2514859. Inactivation, recovery and regulation of the phototransduction cascade.

Miscellaneous databases

PROiP29974.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000067220.
CleanExiMM_CNGA1.
ExpressionAtlasiP29974. baseline and differential.
GenevisibleiP29974. MM.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
InterProiIPR032406. CLZ_dom.
IPR032945. CNGA1.
IPR018490. cNMP-bd-like.
IPR018488. cNMP-bd_CS.
IPR000595. cNMP-bd_dom.
IPR005821. Ion_trans_dom.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PANTHERiPTHR10217:SF387. PTHR10217:SF387. 1 hit.
PfamiPF16526. CLZ. 1 hit.
PF00027. cNMP_binding. 1 hit.
PF00520. Ion_trans. 1 hit.
[Graphical view]
SMARTiSM00100. cNMP. 1 hit.
[Graphical view]
SUPFAMiSSF51206. SSF51206. 1 hit.
PROSITEiPS00888. CNMP_BINDING_1. 1 hit.
PS00889. CNMP_BINDING_2. 1 hit.
PS50042. CNMP_BINDING_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCNGA1_MOUSE
AccessioniPrimary (citable) accession number: P29974
Secondary accession number(s): B9EJ09, Q60776
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 147 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.