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Protein

cGMP-gated cation channel alpha-1

Gene

Cnga1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Visual signal transduction is mediated by a G-protein coupled cascade using cGMP as second messenger. This protein can be activated by cyclic GMP which leads to an opening of the cation channel and thereby causing a depolarization of rod photoreceptors.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei538 – 5381cGMPSequence Analysis
Binding sitei553 – 5531cGMPSequence Analysis

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi479 – 601123cGMPSequence AnalysisAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Ligand-gated ion channel

Keywords - Biological processi

Ion transport, Sensory transduction, Transport, Vision

Keywords - Ligandi

cGMP, cGMP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
cGMP-gated cation channel alpha-1
Alternative name(s):
Cyclic nucleotide-gated cation channel 1
Cyclic nucleotide-gated channel alpha-1
Short name:
CNG channel alpha-1
Short name:
CNG-1
Short name:
CNG1
Cyclic nucleotide-gated channel, photoreceptor
Rod photoreceptor cGMP-gated channel subunit alpha
Gene namesi
Name:Cnga1
Synonyms:Cncg, Cncg1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:88436. Cnga1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 156156CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei157 – 17721Helical; Name=H1Sequence AnalysisAdd
BLAST
Topological domaini178 – 19013ExtracellularSequence AnalysisAdd
BLAST
Transmembranei191 – 20919Helical; Name=H2Sequence AnalysisAdd
BLAST
Topological domaini210 – 23324CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei234 – 25320Helical; Name=H3Sequence AnalysisAdd
BLAST
Topological domaini254 – 29138ExtracellularSequence AnalysisAdd
BLAST
Transmembranei292 – 31423Helical; Name=H4Sequence AnalysisAdd
BLAST
Topological domaini315 – 36652CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei367 – 38620Helical; Name=H5Sequence AnalysisAdd
BLAST
Topological domaini387 – 47084ExtracellularSequence AnalysisAdd
BLAST
Transmembranei471 – 49121Helical; Name=H6Sequence AnalysisAdd
BLAST
Topological domaini492 – 684193CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  • integral component of plasma membrane Source: GO_Central
  • membrane Source: MGI
  • photoreceptor outer segment Source: MGI
  • photoreceptor outer segment membrane Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 684684cGMP-gated cation channel alpha-1PRO_0000219309Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi417 – 4171N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiP29974.

PTM databases

PhosphoSiteiP29974.

Expressioni

Tissue specificityi

Rod cells in the retina and inner medulla of kidney.

Gene expression databases

BgeeiP29974.
CleanExiMM_CNGA1.
GenevisibleiP29974. MM.

Interactioni

Subunit structurei

Tetramer formed of three CNGA1 and one CNGB1 modulatory subunits.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000084464.

Structurei

3D structure databases

ProteinModelPortaliP29974.
SMRiP29974. Positions 393-588, 615-664.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili615 – 65844By similarityAdd
BLAST

Domaini

The C-terminal coiled-coil domain mediates homotrimerization of CNGA subunits.By similarity

Sequence similaritiesi

Contains 1 cyclic nucleotide-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG300025.
GeneTreeiENSGT00760000118772.
HOGENOMiHOG000007898.
HOVERGENiHBG000281.
InParanoidiP29974.
KOiK04948.
OMAiWTMVIAR.
OrthoDBiEOG771268.
PhylomeDBiP29974.
TreeFamiTF319048.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
InterProiIPR018490. cNMP-bd-like.
IPR018488. cNMP-bd_CS.
IPR000595. cNMP-bd_dom.
IPR005821. Ion_trans_dom.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PfamiPF00027. cNMP_binding. 1 hit.
PF00520. Ion_trans. 1 hit.
[Graphical view]
SMARTiSM00100. cNMP. 1 hit.
[Graphical view]
SUPFAMiSSF51206. SSF51206. 1 hit.
PROSITEiPS00888. CNMP_BINDING_1. 1 hit.
PS00889. CNMP_BINDING_2. 1 hit.
PS50042. CNMP_BINDING_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P29974-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTNIINTWH SFVNIPNVIV PAIEKEIRRM ENGACSSFSD DDNGSLSEES
60 70 80 90 100
ENEDSFFRSN SYKRRGPSQR EQHLPGTMAL FNVNNSSNKD QEPKEKKKKK
110 120 130 140 150
KEKKSKADDK NENKKDPEKK KKKEKEKEKK KKEEKTKEKK EEEKKEVVVI
160 170 180 190 200
DPSGNTYYNW LFCITLPVMY NWTMIIARAC FDELQSDYLE YWLIFDYVSD
210 220 230 240 250
VVYLADMFVR TRTGYLEQGL LVKDRMKLIE KYKANLQFKL DVLSVIPTDL
260 270 280 290 300
LYIKFGWNYP EIRLNRLLRI SRMFEFFQRT ETRTNYPNIF RISNLVMYIV
310 320 330 340 350
IIIHWNACVY YSISKAIGFG NDTWVYPDVN DPEFGRLARK YVYSLYWSTL
360 370 380 390 400
TLTTIGETPP PVLDSEYIFV VVDFLIGVLI FATIVGNIGS MISNMNAARA
410 420 430 440 450
EFQSRVDAIK QYMNFRNVSK DMEKRVIKWF DYLWTNKKTV DEREVLRYLP
460 470 480 490 500
DKLRAEIAIN VHLDTLKKVR IFADCEAGLL VELVLKLQPQ VYSPGDYICK
510 520 530 540 550
KGDIGREMYI IKEGKLAVVA DDGITQFVVL SDGSYFGEIS ILNIKGSKAG
560 570 580 590 600
NRRTANIKSI GYSDLFCLSK DDLMEALTEY PDAKTMLEEK GRQILMKDGL
610 620 630 640 650
LDINIANMGS DPKDLEEKVT RMEGSVDLLQ TRFARILAEY ESMQQKLKQR
660 670 680
LTKVEKFLKP LIETEFSALE EPGGESELTE SLQD
Length:684
Mass (Da):79,460
Last modified:November 1, 1997 - v2
Checksum:i13EC2A405B6B8CBF
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti113 – 1142NK → I in AAA37425 (PubMed:1372902).Curated
Sequence conflicti200 – 2001D → N in AAA37425 (PubMed:1372902).Curated
Sequence conflicti576 – 5761A → V in AAA37425 (PubMed:1372902).Curated
Sequence conflicti635 – 6351R → C in AAA37425 (PubMed:1372902).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M84742 mRNA. Translation: AAA37425.1.
U19717 mRNA. Translation: AAA85702.1.
U19715 mRNA. Translation: AAA85700.1.
U19716 mRNA. Translation: AAA85701.1.
BC141261 mRNA. Translation: AAI41262.1.
CCDSiCCDS19333.1.
RefSeqiNP_031749.2. NM_007723.2.
UniGeneiMm.436652.

Genome annotation databases

EnsembliENSMUST00000087213; ENSMUSP00000084464; ENSMUSG00000067220.
ENSMUST00000169997; ENSMUSP00000132329; ENSMUSG00000067220.
GeneIDi12788.
KEGGimmu:12788.
UCSCiuc008xrr.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M84742 mRNA. Translation: AAA37425.1.
U19717 mRNA. Translation: AAA85702.1.
U19715 mRNA. Translation: AAA85700.1.
U19716 mRNA. Translation: AAA85701.1.
BC141261 mRNA. Translation: AAI41262.1.
CCDSiCCDS19333.1.
RefSeqiNP_031749.2. NM_007723.2.
UniGeneiMm.436652.

3D structure databases

ProteinModelPortaliP29974.
SMRiP29974. Positions 393-588, 615-664.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000084464.

PTM databases

PhosphoSiteiP29974.

Proteomic databases

PRIDEiP29974.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000087213; ENSMUSP00000084464; ENSMUSG00000067220.
ENSMUST00000169997; ENSMUSP00000132329; ENSMUSG00000067220.
GeneIDi12788.
KEGGimmu:12788.
UCSCiuc008xrr.1. mouse.

Organism-specific databases

CTDi1259.
MGIiMGI:88436. Cnga1.

Phylogenomic databases

eggNOGiNOG300025.
GeneTreeiENSGT00760000118772.
HOGENOMiHOG000007898.
HOVERGENiHBG000281.
InParanoidiP29974.
KOiK04948.
OMAiWTMVIAR.
OrthoDBiEOG771268.
PhylomeDBiP29974.
TreeFamiTF319048.

Miscellaneous databases

NextBioi282186.
PROiP29974.
SOURCEiSearch...

Gene expression databases

BgeeiP29974.
CleanExiMM_CNGA1.
GenevisibleiP29974. MM.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
InterProiIPR018490. cNMP-bd-like.
IPR018488. cNMP-bd_CS.
IPR000595. cNMP-bd_dom.
IPR005821. Ion_trans_dom.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PfamiPF00027. cNMP_binding. 1 hit.
PF00520. Ion_trans. 1 hit.
[Graphical view]
SMARTiSM00100. cNMP. 1 hit.
[Graphical view]
SUPFAMiSSF51206. SSF51206. 1 hit.
PROSITEiPS00888. CNMP_BINDING_1. 1 hit.
PS00889. CNMP_BINDING_2. 1 hit.
PS50042. CNMP_BINDING_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Primary structure and chromosomal localization of human and mouse rod photoreceptor cGMP-gated cation channel."
    Pittler S.J., Lee A.K., Altherr M.R., Howard T.A., Seldin M.F., Hurwitz R.L., Wasmuth J.J., Baehr W.
    J. Biol. Chem. 267:6257-6262(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Retina.
  2. "Cloning of a cGMP-gated cation channel from mouse kidney inner medullary collecting duct."
    Karlson K.H., Ciampolillo-Bates F., McCoy D.E., Kizer N.L., Stanton B.A.
    Biochim. Biophys. Acta 1236:197-200(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Kidney.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.

Entry informationi

Entry nameiCNGA1_MOUSE
AccessioniPrimary (citable) accession number: P29974
Secondary accession number(s): B9EJ09, Q60776
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: November 1, 1997
Last modified: June 24, 2015
This is version 134 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.