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P29958 (AAC_ACTUT) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Aculeacin-A acylase

EC=3.5.1.-
Gene names
Name:aac
OrganismActinoplanes utahensis
Taxonomic identifier1869 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesMicromonosporineaeMicromonosporaceaeActinoplanes

Protein attributes

Sequence length786 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the hydrolysis of the palmitoyl moiety of the antifungal antibiotic, aculeacin-A, giving a hexapeptide moiety and a long chain fatty acid.

Subunit structure

Heterodimer of a small subunit and a large subunit processed from the same precursor.

Subcellular location

Secreted.

Sequence similarities

Belongs to the peptidase S45 family.

Ontologies

Keywords
   Cellular componentSecreted
   DomainSignal
   Molecular functionHydrolase
   PTMZymogen
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological_processantibiotic biosynthetic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionhydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2222 Potential
Propeptide23 – 3412
PRO_0000020598
Chain35 – 786752Aculeacin-A acylase
PRO_0000253350
Chain35 – 214180Aculeacin-A acylase small subunit
PRO_0000020599
Propeptide215 – 22915Spacer peptide
PRO_0000020600
Chain230 – 786557Aculeacin-A acylase large subunit
PRO_0000020601

Regions

Region35 – 13096Substrate-binding Potential
Region220 – 23920Possible recognition-sequence of an AAC processing enzyme

Sites

Active site2301Nucleophile By similarity

Sequences

Sequence LengthMass (Da)Tools
P29958 [UniParc].

Last modified April 1, 1993. Version 1.
Checksum: 721D8A9D71E4C38C

FASTA78684,090
        10         20         30         40         50         60 
MTSSYMRLKA AAIAFGVIVA TAAVPSPASG REHDGGYAAL IRRASYGVPH ITADDFGSLG 

        70         80         90        100        110        120 
FGVGYVQAED NICVIAESVV TANGERSRWF GATGPDDADV RTTSSTQAID DRVAERLLEG 

       130        140        150        160        170        180 
PRDGVRAPCD DVRDQMRGFV AGYNHFLRRT GVHRLTDPAC RGKAWVRPLS EIDLWRTSWD 

       190        200        210        220        230        240 
SMVRAGSGAL LDGIVAATPP TAAGPASAPE APDAAAIAAA LDGTSAGIGS NAYGLGAQAT 

       250        260        270        280        290        300 
VNGSGMVLAN PHFPWQGAER FYRMHLKVPG RYDVEGAALI GDPIIEIGHN RTVAWSHTVS 

       310        320        330        340        350        360 
TARRFVWHRL SLVPGDPTSY YVDGRPERMR ARTVTVQTGS GPVSRTFHDT RYGPVAVVPG 

       370        380        390        400        410        420 
TFDWTPATAY AITDVNAGNN RAFDGWLRMG QAKDVRALKA VLDRHQFLPW VNVIAADARG 

       430        440        450        460        470        480 
EALYGDHSVV PRVTGALAAA CIPAPFQPLY ASSGQAVLDG SRSDCALGAD PDAAVPGILG 

       490        500        510        520        530        540 
PASLPVRFRD DYVTNSNDSH WLASPAAPLE GFPRILGNER TPRSLRTRLG LDQIQQRLAG 

       550        560        570        580        590        600 
TDGLPGKGFT TARLWQVMFG NRMHGAELVR DDLVALCRRQ PTATASNGAI VDLTAACTAL 

       610        620        630        640        650        660 
SRFDERADLD SRGAHLFTEF LAGGIRFADT FEVTDPVRTP APFWNTTDPR VRTALADACN 

       670        680        690        700        710        720 
GSPASPSTRS VGDIHTDSRG ERRIPIHGGR GEAGTFNVIT NPLVPGVGYP QVVHGTSFVM 

       730        740        750        760        770        780 
AVELGPHGPS GRQILTYAQS TNPNSPWYAD QTVLYSRKGW DTIKYTEAQI AADPNLRVYR 


VAQRGR 

« Hide

References

[1]"Cloning and sequencing of the aculeacin A acylase-encoding gene from Actinoplanes utahensis and expression in Streptomyces lividans."
Inokoshi J., Takeshima H., Ikeda H., Omura S.
Gene 119:29-35(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 35-53; 212-214 AND 230-249.
Strain: NRRL 12052.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D10610 Genomic DNA. Translation: BAA01465.1.
PIRJC1298.

3D structure databases

ProteinModelPortalP29958.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D1.10.439.10. 1 hit.
InterProIPR002692. Penicillin_amidase-like.
IPR023343. Penicillin_amidase_dom1.
[Graphical view]
PfamPF01804. Penicil_amidase. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameAAC_ACTUT
AccessionPrimary (citable) accession number: P29958
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: May 1, 2013
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries