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P29958

- AAC_ACTUT

UniProt

P29958 - AAC_ACTUT

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Protein
Aculeacin-A acylase
Gene
aac
Organism
Actinoplanes utahensis
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the hydrolysis of the palmitoyl moiety of the antifungal antibiotic, aculeacin-A, giving a hexapeptide moiety and a long chain fatty acid.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei230 – 2301Nucleophile By similarity

GO - Molecular functioni

  1. hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides Source: InterPro

GO - Biological processi

  1. antibiotic biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
Aculeacin-A acylase (EC:3.5.1.-)
Cleaved into the following 2 chains:
Gene namesi
Name:aac
OrganismiActinoplanes utahensis
Taxonomic identifieri1869 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesMicromonosporineaeMicromonosporaceaeActinoplanes

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222 Reviewed prediction
Add
BLAST
Propeptidei23 – 3412
PRO_0000020598Add
BLAST
Chaini35 – 786752Aculeacin-A acylase
PRO_0000253350Add
BLAST
Chaini35 – 214180Aculeacin-A acylase small subunit
PRO_0000020599Add
BLAST
Propeptidei215 – 22915Spacer peptide
PRO_0000020600Add
BLAST
Chaini230 – 786557Aculeacin-A acylase large subunit
PRO_0000020601Add
BLAST

Keywords - PTMi

Zymogen

Interactioni

Subunit structurei

Heterodimer of a small subunit and a large subunit processed from the same precursor.

Structurei

3D structure databases

ProteinModelPortaliP29958.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni35 – 13096Substrate-binding Reviewed prediction
Add
BLAST
Regioni220 – 23920Possible recognition-sequence of an AAC processing enzyme
Add
BLAST

Sequence similaritiesi

Belongs to the peptidase S45 family.

Keywords - Domaini

Signal

Family and domain databases

Gene3Di1.10.439.10. 1 hit.
3.60.20.10. 2 hits.
InterProiIPR029055. Ntn_hydrolases_N.
IPR002692. Penicillin_amidase-like.
IPR023343. Penicillin_amidase_dom1.
[Graphical view]
PfamiPF01804. Penicil_amidase. 1 hit.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P29958-1 [UniParc]FASTAAdd to Basket

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MTSSYMRLKA AAIAFGVIVA TAAVPSPASG REHDGGYAAL IRRASYGVPH    50
ITADDFGSLG FGVGYVQAED NICVIAESVV TANGERSRWF GATGPDDADV 100
RTTSSTQAID DRVAERLLEG PRDGVRAPCD DVRDQMRGFV AGYNHFLRRT 150
GVHRLTDPAC RGKAWVRPLS EIDLWRTSWD SMVRAGSGAL LDGIVAATPP 200
TAAGPASAPE APDAAAIAAA LDGTSAGIGS NAYGLGAQAT VNGSGMVLAN 250
PHFPWQGAER FYRMHLKVPG RYDVEGAALI GDPIIEIGHN RTVAWSHTVS 300
TARRFVWHRL SLVPGDPTSY YVDGRPERMR ARTVTVQTGS GPVSRTFHDT 350
RYGPVAVVPG TFDWTPATAY AITDVNAGNN RAFDGWLRMG QAKDVRALKA 400
VLDRHQFLPW VNVIAADARG EALYGDHSVV PRVTGALAAA CIPAPFQPLY 450
ASSGQAVLDG SRSDCALGAD PDAAVPGILG PASLPVRFRD DYVTNSNDSH 500
WLASPAAPLE GFPRILGNER TPRSLRTRLG LDQIQQRLAG TDGLPGKGFT 550
TARLWQVMFG NRMHGAELVR DDLVALCRRQ PTATASNGAI VDLTAACTAL 600
SRFDERADLD SRGAHLFTEF LAGGIRFADT FEVTDPVRTP APFWNTTDPR 650
VRTALADACN GSPASPSTRS VGDIHTDSRG ERRIPIHGGR GEAGTFNVIT 700
NPLVPGVGYP QVVHGTSFVM AVELGPHGPS GRQILTYAQS TNPNSPWYAD 750
QTVLYSRKGW DTIKYTEAQI AADPNLRVYR VAQRGR 786
Length:786
Mass (Da):84,090
Last modified:April 1, 1993 - v1
Checksum:i721D8A9D71E4C38C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D10610 Genomic DNA. Translation: BAA01465.1.
PIRiJC1298.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D10610 Genomic DNA. Translation: BAA01465.1 .
PIRi JC1298.

3D structure databases

ProteinModelPortali P29958.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 1.10.439.10. 1 hit.
3.60.20.10. 2 hits.
InterProi IPR029055. Ntn_hydrolases_N.
IPR002692. Penicillin_amidase-like.
IPR023343. Penicillin_amidase_dom1.
[Graphical view ]
Pfami PF01804. Penicil_amidase. 1 hit.
[Graphical view ]
SUPFAMi SSF56235. SSF56235. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Cloning and sequencing of the aculeacin A acylase-encoding gene from Actinoplanes utahensis and expression in Streptomyces lividans."
    Inokoshi J., Takeshima H., Ikeda H., Omura S.
    Gene 119:29-35(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 35-53; 212-214 AND 230-249.
    Strain: NRRL 12052.

Entry informationi

Entry nameiAAC_ACTUT
AccessioniPrimary (citable) accession number: P29958
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: June 11, 2014
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi