Reviewed, UniProtKB/Swiss-Prot P29958 (AAC_ACTUT)

Names and origin

Protein names

Recommended name:
    Aculeacin-A acylase
    EC=3.5.1.-
Cleaved into the following 2 chains:
    1- Recommended name:
            Aculeacin-A acylase small subunit
    2- Recommended name:
            Aculeacin-A acylase large subunit

Gene names

Name:

aac

Organism

Actinoplanes utahensis

Taxonomic identifier

1869 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micromonosporineae › Micromonosporaceae › Actinoplanes

Protein attributes

Sequence length	786 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the hydrolysis of the palmitoyl moiety of the antifungal antibiotic, aculeacin-A, giving a hexapeptide moiety and a long chain fatty acid.
Subunit structure	Heterodimer of a small subunit and a large subunit processed from the same precursor.
Subcellular location	Secreted.
Sequence similarities	Belongs to the peptidase S45 family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase
PTM	Zymogen
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	antibiotic biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	hydrolase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Propeptide	23 – 34	12			PRO_0000020598
	Chain	35 – 786	752	Aculeacin-A acylase		PRO_0000253350
	Chain	35 – 214	180	Aculeacin-A acylase small subunit		PRO_0000020599
	Propeptide	215 – 229	15	Spacer peptide		PRO_0000020600
	Chain	230 – 786	557	Aculeacin-A acylase large subunit		PRO_0000020601
Regions
	Region	35 – 130	96	Substrate-binding Potential
	Region	220 – 239	20	Possible recognition-sequence of an AAC processing enzyme
Sites
	Active site	230	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	P29958-1 [UniParc]. Last modified April 1, 1993. Version 1. Checksum: 721D8A9D71E4C38C	FASTA	786	84,090
	10 20 30 40 50 60 MTSSYMRLKA AAIAFGVIVA TAAVPSPASG REHDGGYAAL IRRASYGVPH ITADDFGSLG 70 80 90 100 110 120 FGVGYVQAED NICVIAESVV TANGERSRWF GATGPDDADV RTTSSTQAID DRVAERLLEG 130 140 150 160 170 180 PRDGVRAPCD DVRDQMRGFV AGYNHFLRRT GVHRLTDPAC RGKAWVRPLS EIDLWRTSWD 190 200 210 220 230 240 SMVRAGSGAL LDGIVAATPP TAAGPASAPE APDAAAIAAA LDGTSAGIGS NAYGLGAQAT 250 260 270 280 290 300 VNGSGMVLAN PHFPWQGAER FYRMHLKVPG RYDVEGAALI GDPIIEIGHN RTVAWSHTVS 310 320 330 340 350 360 TARRFVWHRL SLVPGDPTSY YVDGRPERMR ARTVTVQTGS GPVSRTFHDT RYGPVAVVPG 370 380 390 400 410 420 TFDWTPATAY AITDVNAGNN RAFDGWLRMG QAKDVRALKA VLDRHQFLPW VNVIAADARG 430 440 450 460 470 480 EALYGDHSVV PRVTGALAAA CIPAPFQPLY ASSGQAVLDG SRSDCALGAD PDAAVPGILG 490 500 510 520 530 540 PASLPVRFRD DYVTNSNDSH WLASPAAPLE GFPRILGNER TPRSLRTRLG LDQIQQRLAG 550 560 570 580 590 600 TDGLPGKGFT TARLWQVMFG NRMHGAELVR DDLVALCRRQ PTATASNGAI VDLTAACTAL 610 620 630 640 650 660 SRFDERADLD SRGAHLFTEF LAGGIRFADT FEVTDPVRTP APFWNTTDPR VRTALADACN 670 680 690 700 710 720 GSPASPSTRS VGDIHTDSRG ERRIPIHGGR GEAGTFNVIT NPLVPGVGYP QVVHGTSFVM 730 740 750 760 770 780 AVELGPHGPS GRQILTYAQS TNPNSPWYAD QTVLYSRKGW DTIKYTEAQI AADPNLRVYR VAQRGR

References

[1]

"Cloning and sequencing of the aculeacin A acylase-encoding gene from Actinoplanes utahensis and expression in Streptomyces lividans." Inokoshi J., Takeshima H., Ikeda H., Omura S. Gene 119:29-35(1992) [PubMed: 1398088] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 35-53; 212-214 AND 230-249. Strain: NRRL 12052.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D10610 Genomic DNA. Translation: BAA01465.1.
PIR	JC1298.
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR002692. Peptidase_S45. [Graphical view]
Pfam	PF01804. Penicil_amidase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AAC_ACTUT

Accession

Primary (citable) accession number: P29958

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	June 16, 2009
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)