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P29958

- AAC_ACTUT

UniProt

P29958 - AAC_ACTUT

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Protein

Aculeacin-A acylase

Gene

aac

Organism
Actinoplanes utahensis
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the hydrolysis of the palmitoyl moiety of the antifungal antibiotic, aculeacin-A, giving a hexapeptide moiety and a long chain fatty acid.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei230 – 2301NucleophileBy similarity

GO - Molecular functioni

  1. hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides Source: InterPro

GO - Biological processi

  1. antibiotic biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
Aculeacin-A acylase (EC:3.5.1.-)
Cleaved into the following 2 chains:
Gene namesi
Name:aac
OrganismiActinoplanes utahensis
Taxonomic identifieri1869 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesMicromonosporineaeMicromonosporaceaeActinoplanes

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Sequence AnalysisAdd
BLAST
Propeptidei23 – 34121 PublicationPRO_0000020598Add
BLAST
Chaini35 – 786752Aculeacin-A acylasePRO_0000253350Add
BLAST
Chaini35 – 214180Aculeacin-A acylase small subunitPRO_0000020599Add
BLAST
Propeptidei215 – 22915Spacer peptide1 PublicationPRO_0000020600Add
BLAST
Chaini230 – 786557Aculeacin-A acylase large subunitPRO_0000020601Add
BLAST

Keywords - PTMi

Zymogen

Interactioni

Subunit structurei

Heterodimer of a small subunit and a large subunit processed from the same precursor.

Structurei

3D structure databases

ProteinModelPortaliP29958.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni35 – 13096Substrate-bindingSequence AnalysisAdd
BLAST
Regioni220 – 23920Possible recognition-sequence of an AAC processing enzymeAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S45 family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di1.10.439.10. 1 hit.
3.60.20.10. 2 hits.
InterProiIPR029055. Ntn_hydrolases_N.
IPR002692. Penicillin_amidase-like.
IPR023343. Penicillin_amidase_dom1.
[Graphical view]
PfamiPF01804. Penicil_amidase. 1 hit.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P29958-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTSSYMRLKA AAIAFGVIVA TAAVPSPASG REHDGGYAAL IRRASYGVPH
60 70 80 90 100
ITADDFGSLG FGVGYVQAED NICVIAESVV TANGERSRWF GATGPDDADV
110 120 130 140 150
RTTSSTQAID DRVAERLLEG PRDGVRAPCD DVRDQMRGFV AGYNHFLRRT
160 170 180 190 200
GVHRLTDPAC RGKAWVRPLS EIDLWRTSWD SMVRAGSGAL LDGIVAATPP
210 220 230 240 250
TAAGPASAPE APDAAAIAAA LDGTSAGIGS NAYGLGAQAT VNGSGMVLAN
260 270 280 290 300
PHFPWQGAER FYRMHLKVPG RYDVEGAALI GDPIIEIGHN RTVAWSHTVS
310 320 330 340 350
TARRFVWHRL SLVPGDPTSY YVDGRPERMR ARTVTVQTGS GPVSRTFHDT
360 370 380 390 400
RYGPVAVVPG TFDWTPATAY AITDVNAGNN RAFDGWLRMG QAKDVRALKA
410 420 430 440 450
VLDRHQFLPW VNVIAADARG EALYGDHSVV PRVTGALAAA CIPAPFQPLY
460 470 480 490 500
ASSGQAVLDG SRSDCALGAD PDAAVPGILG PASLPVRFRD DYVTNSNDSH
510 520 530 540 550
WLASPAAPLE GFPRILGNER TPRSLRTRLG LDQIQQRLAG TDGLPGKGFT
560 570 580 590 600
TARLWQVMFG NRMHGAELVR DDLVALCRRQ PTATASNGAI VDLTAACTAL
610 620 630 640 650
SRFDERADLD SRGAHLFTEF LAGGIRFADT FEVTDPVRTP APFWNTTDPR
660 670 680 690 700
VRTALADACN GSPASPSTRS VGDIHTDSRG ERRIPIHGGR GEAGTFNVIT
710 720 730 740 750
NPLVPGVGYP QVVHGTSFVM AVELGPHGPS GRQILTYAQS TNPNSPWYAD
760 770 780
QTVLYSRKGW DTIKYTEAQI AADPNLRVYR VAQRGR
Length:786
Mass (Da):84,090
Last modified:April 1, 1993 - v1
Checksum:i721D8A9D71E4C38C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D10610 Genomic DNA. Translation: BAA01465.1.
PIRiJC1298.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D10610 Genomic DNA. Translation: BAA01465.1 .
PIRi JC1298.

3D structure databases

ProteinModelPortali P29958.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 1.10.439.10. 1 hit.
3.60.20.10. 2 hits.
InterProi IPR029055. Ntn_hydrolases_N.
IPR002692. Penicillin_amidase-like.
IPR023343. Penicillin_amidase_dom1.
[Graphical view ]
Pfami PF01804. Penicil_amidase. 1 hit.
[Graphical view ]
SUPFAMi SSF56235. SSF56235. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Cloning and sequencing of the aculeacin A acylase-encoding gene from Actinoplanes utahensis and expression in Streptomyces lividans."
    Inokoshi J., Takeshima H., Ikeda H., Omura S.
    Gene 119:29-35(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 35-53; 212-214 AND 230-249.
    Strain: NRRL 12052.

Entry informationi

Entry nameiAAC_ACTUT
AccessioniPrimary (citable) accession number: P29958
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: October 29, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3