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Protein

Mannose-6-phosphate isomerase

Gene

PMI40

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions.

Miscellaneous

Present with 5220 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

D-mannose 6-phosphate = D-fructose 6-phosphate.

Cofactori

Zn2+Note: Binds 1 zinc ion per subunit.

Enzyme regulationi

Can be inhibited by an excess of zinc.

Pathwayi: GDP-alpha-D-mannose biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes alpha-D-mannose 1-phosphate from D-fructose 6-phosphate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Mannose-6-phosphate isomerase (PMI40)
  2. Phosphomannomutase (SEC53)
This subpathway is part of the pathway GDP-alpha-D-mannose biosynthesis, which is itself part of Nucleotide-sugar biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes alpha-D-mannose 1-phosphate from D-fructose 6-phosphate, the pathway GDP-alpha-D-mannose biosynthesis and in Nucleotide-sugar biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi109ZincBy similarity1
Metal bindingi111ZincBy similarity1
Metal bindingi136ZincBy similarity1
Metal bindingi281ZincBy similarity1
Active sitei300By similarity1

GO - Molecular functioni

  • mannose-6-phosphate isomerase activity Source: SGD
  • zinc ion binding Source: InterPro

GO - Biological processi

  • carbohydrate metabolic process Source: InterPro
  • cell wall mannoprotein biosynthetic process Source: SGD
  • fungal-type cell wall organization Source: GO_Central
  • GDP-mannose biosynthetic process Source: SGD
  • protein glycosylation Source: SGD

Keywordsi

Molecular functionIsomerase
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:YER003C-MONOMER
ReactomeiR-SCE-446205 Synthesis of GDP-mannose
SABIO-RKiP29952
UniPathwayiUPA00126; UER00423

Names & Taxonomyi

Protein namesi
Recommended name:
Mannose-6-phosphate isomerase (EC:5.3.1.8)
Alternative name(s):
Phosphohexomutase
Phosphomannose isomerase
Short name:
PMI
Gene namesi
Name:PMI40
Ordered Locus Names:YER003C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YER003C
SGDiS000000805 PMI40

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001942472 – 429Mannose-6-phosphate isomeraseAdd BLAST428

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserine1 Publication1
Modified residuei107PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP29952
PaxDbiP29952
PRIDEiP29952

2D gel databases

UCD-2DPAGEiP29952

PTM databases

CarbonylDBiP29952
iPTMnetiP29952

Expressioni

Inductioni

By D-mannose.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

BioGridi36733389 interactors.
DIPiDIP-6619N
IntActiP29952 7 interactors.
MINTiP29952
STRINGi4932.YER003C

Structurei

3D structure databases

ProteinModelPortaliP29952
SMRiP29952
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000016075
HOGENOMiHOG000241277
InParanoidiP29952
KOiK01809
OMAiMGTHPSN
OrthoDBiEOG092C3NWF

Family and domain databases

Gene3Di2.60.120.104 hits
InterProiView protein in InterPro
IPR001250 Man6P_Isoase-1
IPR016305 Mannose-6-P_Isomerase
IPR018050 Pmannose_isomerase-type1_CS
IPR014710 RmlC-like_jellyroll
IPR011051 RmlC_Cupin_sf
PANTHERiPTHR10309 PTHR10309, 1 hit
PfamiView protein in Pfam
PF01238 PMI_typeI, 1 hit
PIRSFiPIRSF001480 Mannose-6-phosphate_isomerase, 1 hit
PRINTSiPR00714 MAN6PISMRASE
SUPFAMiSSF51182 SSF51182, 1 hit
TIGRFAMsiTIGR00218 manA, 1 hit
PROSITEiView protein in PROSITE
PS00965 PMI_I_1, 1 hit
PS00966 PMI_I_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P29952-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNKLFRLDA GYQQYDWGKI GSSSAVAQFA AHSDPSVQIE QDKPYAELWM
60 70 80 90 100
GTHSKMPSYN HESKESLRDI ISKNPSAMLG KDIIDKFHAT NELPFLFKVL
110 120 130 140 150
SIEKVLSIQA HPDKALGKIL HAQDPKNYPD DNHKPEMAIA VTDFEGFCGF
160 170 180 190 200
KPLQEIADEL KRIPELRNIV GEETSRNFIE NIQPSAQKGS PEDEQNKKLL
210 220 230 240 250
QAVFSRVMNA SDDKIKIQAR SLVERSKNSP SDFNKPDLPE LIQRLNKQFP
260 270 280 290 300
DDVGLFCGCL LLNHCRLNAG EAIFLRAKDP HAYISGDIME CMAASDNVVR
310 320 330 340 350
AGFTPKFKDV KNLVSMLTYT YDPVEKQKMQ PLKFDRSSGN GKSVLYNPPI
360 370 380 390 400
EEFAVLETTF DEKLGQRHFE GVDGPSILIT TKGNGYIKAD GQKLKAEPGF
410 420
VFFIAPHLPV DLEAEDEAFT TYRAFVEPN
Length:429
Mass (Da):48,189
Last modified:January 23, 2007 - v4
Checksum:iC95E7C06B348A02F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti25A → R in AAA34872 (PubMed:1377774).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M85238 Genomic DNA Translation: AAA34872.1
U18778 Genomic DNA Translation: AAB64536.1
BK006939 Genomic DNA Translation: DAA07653.1
PIRiS50461
RefSeqiNP_010918.1, NM_001178894.1

Genome annotation databases

EnsemblFungiiYER003C; YER003C; YER003C
GeneIDi856720
KEGGisce:YER003C

Similar proteinsi

Entry informationi

Entry nameiMPI_YEAST
AccessioniPrimary (citable) accession number: P29952
Secondary accession number(s): D3DLP9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: January 23, 2007
Last modified: March 28, 2018
This is version 163 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome