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P29952

- MPI_YEAST

UniProt

P29952 - MPI_YEAST

Protein

Mannose-6-phosphate isomerase

Gene

PMI40

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 134 (01 Oct 2014)
      Sequence version 4 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions.

    Catalytic activityi

    D-mannose 6-phosphate = D-fructose 6-phosphate.

    Cofactori

    Binds 1 zinc ion per subunit.

    Enzyme regulationi

    Can be inhibited by an excess of zinc.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi109 – 1091ZincBy similarity
    Metal bindingi111 – 1111ZincBy similarity
    Metal bindingi136 – 1361ZincBy similarity
    Metal bindingi281 – 2811ZincBy similarity
    Active sitei300 – 3001By similarity

    GO - Molecular functioni

    1. mannose-6-phosphate isomerase activity Source: SGD
    2. zinc ion binding Source: InterPro

    GO - Biological processi

    1. cell wall mannoprotein biosynthetic process Source: SGD
    2. GDP-mannose biosynthetic process Source: SGD
    3. protein glycosylation Source: SGD

    Keywords - Molecular functioni

    Isomerase

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciYEAST:YER003C-MONOMER.
    ReactomeiREACT_189212. Synthesis of GDP-mannose.
    SABIO-RKP29952.
    UniPathwayiUPA00126; UER00423.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mannose-6-phosphate isomerase (EC:5.3.1.8)
    Alternative name(s):
    Phosphohexomutase
    Phosphomannose isomerase
    Short name:
    PMI
    Gene namesi
    Name:PMI40
    Ordered Locus Names:YER003C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome V

    Organism-specific databases

    SGDiS000000805. PMI40.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 429428Mannose-6-phosphate isomerasePRO_0000194247Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserine1 Publication
    Modified residuei107 – 1071Phosphoserine1 Publication

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiP29952.
    PaxDbiP29952.
    PeptideAtlasiP29952.

    2D gel databases

    UCD-2DPAGEP29952.

    Expressioni

    Inductioni

    By D-mannose.

    Gene expression databases

    GenevestigatoriP29952.

    Interactioni

    Subunit structurei

    Monomer.

    Protein-protein interaction databases

    BioGridi36733. 18 interactions.
    DIPiDIP-6619N.
    MINTiMINT-2786830.
    STRINGi4932.YER003C.

    Structurei

    3D structure databases

    ProteinModelPortaliP29952.
    SMRiP29952. Positions 2-427.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG1482.
    GeneTreeiENSGT00390000016075.
    HOGENOMiHOG000241277.
    KOiK01809.
    OMAiHANGERC.
    OrthoDBiEOG7WDNCR.

    Family and domain databases

    Gene3Di2.60.120.10. 3 hits.
    InterProiIPR001250. Man6P_Isoase-1.
    IPR016305. Mannose-6-P_Isomerase.
    IPR018050. Pmannose_isomerase-type1_CS.
    IPR014710. RmlC-like_jellyroll.
    IPR011051. RmlC_Cupin.
    [Graphical view]
    PANTHERiPTHR10309. PTHR10309. 1 hit.
    PfamiPF01238. PMI_typeI. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001480. Mannose-6-phosphate_isomerase. 1 hit.
    PRINTSiPR00714. MAN6PISMRASE.
    SUPFAMiSSF51182. SSF51182. 1 hit.
    TIGRFAMsiTIGR00218. manA. 1 hit.
    PROSITEiPS00965. PMI_I_1. 1 hit.
    PS00966. PMI_I_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P29952-1 [UniParc]FASTAAdd to Basket

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    MSNKLFRLDA GYQQYDWGKI GSSSAVAQFA AHSDPSVQIE QDKPYAELWM    50
    GTHSKMPSYN HESKESLRDI ISKNPSAMLG KDIIDKFHAT NELPFLFKVL 100
    SIEKVLSIQA HPDKALGKIL HAQDPKNYPD DNHKPEMAIA VTDFEGFCGF 150
    KPLQEIADEL KRIPELRNIV GEETSRNFIE NIQPSAQKGS PEDEQNKKLL 200
    QAVFSRVMNA SDDKIKIQAR SLVERSKNSP SDFNKPDLPE LIQRLNKQFP 250
    DDVGLFCGCL LLNHCRLNAG EAIFLRAKDP HAYISGDIME CMAASDNVVR 300
    AGFTPKFKDV KNLVSMLTYT YDPVEKQKMQ PLKFDRSSGN GKSVLYNPPI 350
    EEFAVLETTF DEKLGQRHFE GVDGPSILIT TKGNGYIKAD GQKLKAEPGF 400
    VFFIAPHLPV DLEAEDEAFT TYRAFVEPN 429
    Length:429
    Mass (Da):48,189
    Last modified:January 23, 2007 - v4
    Checksum:iC95E7C06B348A02F
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti25 – 251A → R in AAA34872. (PubMed:1377774)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M85238 Genomic DNA. Translation: AAA34872.1.
    U18778 Genomic DNA. Translation: AAB64536.1.
    BK006939 Genomic DNA. Translation: DAA07653.1.
    PIRiS50461.
    RefSeqiNP_010918.1. NM_001178894.1.

    Genome annotation databases

    EnsemblFungiiYER003C; YER003C; YER003C.
    GeneIDi856720.
    KEGGisce:YER003C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M85238 Genomic DNA. Translation: AAA34872.1 .
    U18778 Genomic DNA. Translation: AAB64536.1 .
    BK006939 Genomic DNA. Translation: DAA07653.1 .
    PIRi S50461.
    RefSeqi NP_010918.1. NM_001178894.1.

    3D structure databases

    ProteinModelPortali P29952.
    SMRi P29952. Positions 2-427.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36733. 18 interactions.
    DIPi DIP-6619N.
    MINTi MINT-2786830.
    STRINGi 4932.YER003C.

    2D gel databases

    UCD-2DPAGE P29952.

    Proteomic databases

    MaxQBi P29952.
    PaxDbi P29952.
    PeptideAtlasi P29952.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YER003C ; YER003C ; YER003C .
    GeneIDi 856720.
    KEGGi sce:YER003C.

    Organism-specific databases

    SGDi S000000805. PMI40.

    Phylogenomic databases

    eggNOGi COG1482.
    GeneTreei ENSGT00390000016075.
    HOGENOMi HOG000241277.
    KOi K01809.
    OMAi HANGERC.
    OrthoDBi EOG7WDNCR.

    Enzyme and pathway databases

    UniPathwayi UPA00126 ; UER00423 .
    BioCyci YEAST:YER003C-MONOMER.
    Reactomei REACT_189212. Synthesis of GDP-mannose.
    SABIO-RK P29952.

    Miscellaneous databases

    NextBioi 982811.
    PROi P29952.

    Gene expression databases

    Genevestigatori P29952.

    Family and domain databases

    Gene3Di 2.60.120.10. 3 hits.
    InterProi IPR001250. Man6P_Isoase-1.
    IPR016305. Mannose-6-P_Isomerase.
    IPR018050. Pmannose_isomerase-type1_CS.
    IPR014710. RmlC-like_jellyroll.
    IPR011051. RmlC_Cupin.
    [Graphical view ]
    PANTHERi PTHR10309. PTHR10309. 1 hit.
    Pfami PF01238. PMI_typeI. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001480. Mannose-6-phosphate_isomerase. 1 hit.
    PRINTSi PR00714. MAN6PISMRASE.
    SUPFAMi SSF51182. SSF51182. 1 hit.
    TIGRFAMsi TIGR00218. manA. 1 hit.
    PROSITEi PS00965. PMI_I_1. 1 hit.
    PS00966. PMI_I_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "PMI40, an intron-containing gene required for early steps in yeast mannosylation."
      Smith D.J., Proudfoot A.E.I., Friedli L., Klig L.S., Paravicini G., Payton M.A.
      Mol. Cell. Biol. 12:2924-2930(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT SER-2.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "Phosphomannose isomerase from Saccharomyces cerevisiae contains two inhibitory metal ion binding sites."
      Wells T.N.C., Coulin F., Payton M.A., Proudfoot A.E.I.
      Biochemistry 32:1294-1301(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: INHIBITION BY ZINC.
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    6. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-107, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiMPI_YEAST
    AccessioniPrimary (citable) accession number: P29952
    Secondary accession number(s): D3DLP9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1993
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 134 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 5220 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome V
      Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

    External Data

    Dasty 3