SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P29952

- MPI_YEAST

UniProt

P29952 - MPI_YEAST

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Mannose-6-phosphate isomerase
Gene
PMI40, YER003C
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions.

Catalytic activityi

D-mannose 6-phosphate = D-fructose 6-phosphate.

Cofactori

Binds 1 zinc ion per subunit.

Enzyme regulationi

Can be inhibited by an excess of zinc.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi109 – 1091Zinc By similarity
Metal bindingi111 – 1111Zinc By similarity
Metal bindingi136 – 1361Zinc By similarity
Metal bindingi281 – 2811Zinc By similarity
Active sitei300 – 3001 By similarity

GO - Molecular functioni

  1. mannose-6-phosphate isomerase activity Source: SGD
  2. zinc ion binding Source: InterPro

GO - Biological processi

  1. GDP-mannose biosynthetic process Source: SGD
  2. cell wall mannoprotein biosynthetic process Source: SGD
  3. protein glycosylation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:YER003C-MONOMER.
ReactomeiREACT_189212. Synthesis of GDP-mannose.
SABIO-RKP29952.
UniPathwayiUPA00126; UER00423.

Names & Taxonomyi

Protein namesi
Recommended name:
Mannose-6-phosphate isomerase (EC:5.3.1.8)
Alternative name(s):
Phosphohexomutase
Phosphomannose isomerase
Short name:
PMI
Gene namesi
Name:PMI40
Ordered Locus Names:YER003C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome V

Organism-specific databases

SGDiS000000805. PMI40.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 429428Mannose-6-phosphate isomerase
PRO_0000194247Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine1 Publication
Modified residuei107 – 1071Phosphoserine1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP29952.
PaxDbiP29952.
PeptideAtlasiP29952.

2D gel databases

UCD-2DPAGEP29952.

Expressioni

Inductioni

By D-mannose.

Gene expression databases

GenevestigatoriP29952.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

BioGridi36733. 18 interactions.
DIPiDIP-6619N.
MINTiMINT-2786830.
STRINGi4932.YER003C.

Structurei

3D structure databases

ProteinModelPortaliP29952.
SMRiP29952. Positions 2-427.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1482.
GeneTreeiENSGT00390000016075.
HOGENOMiHOG000241277.
KOiK01809.
OMAiHANGERC.
OrthoDBiEOG7WDNCR.

Family and domain databases

Gene3Di2.60.120.10. 3 hits.
InterProiIPR001250. Man6P_Isoase-1.
IPR016305. Mannose-6-P_Isomerase.
IPR018050. Pmannose_isomerase-type1_CS.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PANTHERiPTHR10309. PTHR10309. 1 hit.
PfamiPF01238. PMI_typeI. 1 hit.
[Graphical view]
PIRSFiPIRSF001480. Mannose-6-phosphate_isomerase. 1 hit.
PRINTSiPR00714. MAN6PISMRASE.
SUPFAMiSSF51182. SSF51182. 1 hit.
TIGRFAMsiTIGR00218. manA. 1 hit.
PROSITEiPS00965. PMI_I_1. 1 hit.
PS00966. PMI_I_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P29952-1 [UniParc]FASTAAdd to Basket

« Hide

MSNKLFRLDA GYQQYDWGKI GSSSAVAQFA AHSDPSVQIE QDKPYAELWM    50
GTHSKMPSYN HESKESLRDI ISKNPSAMLG KDIIDKFHAT NELPFLFKVL 100
SIEKVLSIQA HPDKALGKIL HAQDPKNYPD DNHKPEMAIA VTDFEGFCGF 150
KPLQEIADEL KRIPELRNIV GEETSRNFIE NIQPSAQKGS PEDEQNKKLL 200
QAVFSRVMNA SDDKIKIQAR SLVERSKNSP SDFNKPDLPE LIQRLNKQFP 250
DDVGLFCGCL LLNHCRLNAG EAIFLRAKDP HAYISGDIME CMAASDNVVR 300
AGFTPKFKDV KNLVSMLTYT YDPVEKQKMQ PLKFDRSSGN GKSVLYNPPI 350
EEFAVLETTF DEKLGQRHFE GVDGPSILIT TKGNGYIKAD GQKLKAEPGF 400
VFFIAPHLPV DLEAEDEAFT TYRAFVEPN 429
Length:429
Mass (Da):48,189
Last modified:January 23, 2007 - v4
Checksum:iC95E7C06B348A02F
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti25 – 251A → R in AAA34872. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M85238 Genomic DNA. Translation: AAA34872.1.
U18778 Genomic DNA. Translation: AAB64536.1.
BK006939 Genomic DNA. Translation: DAA07653.1.
PIRiS50461.
RefSeqiNP_010918.1. NM_001178894.1.

Genome annotation databases

EnsemblFungiiYER003C; YER003C; YER003C.
GeneIDi856720.
KEGGisce:YER003C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M85238 Genomic DNA. Translation: AAA34872.1 .
U18778 Genomic DNA. Translation: AAB64536.1 .
BK006939 Genomic DNA. Translation: DAA07653.1 .
PIRi S50461.
RefSeqi NP_010918.1. NM_001178894.1.

3D structure databases

ProteinModelPortali P29952.
SMRi P29952. Positions 2-427.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 36733. 18 interactions.
DIPi DIP-6619N.
MINTi MINT-2786830.
STRINGi 4932.YER003C.

2D gel databases

UCD-2DPAGE P29952.

Proteomic databases

MaxQBi P29952.
PaxDbi P29952.
PeptideAtlasi P29952.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YER003C ; YER003C ; YER003C .
GeneIDi 856720.
KEGGi sce:YER003C.

Organism-specific databases

SGDi S000000805. PMI40.

Phylogenomic databases

eggNOGi COG1482.
GeneTreei ENSGT00390000016075.
HOGENOMi HOG000241277.
KOi K01809.
OMAi HANGERC.
OrthoDBi EOG7WDNCR.

Enzyme and pathway databases

UniPathwayi UPA00126 ; UER00423 .
BioCyci YEAST:YER003C-MONOMER.
Reactomei REACT_189212. Synthesis of GDP-mannose.
SABIO-RK P29952.

Miscellaneous databases

NextBioi 982811.
PROi P29952.

Gene expression databases

Genevestigatori P29952.

Family and domain databases

Gene3Di 2.60.120.10. 3 hits.
InterProi IPR001250. Man6P_Isoase-1.
IPR016305. Mannose-6-P_Isomerase.
IPR018050. Pmannose_isomerase-type1_CS.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view ]
PANTHERi PTHR10309. PTHR10309. 1 hit.
Pfami PF01238. PMI_typeI. 1 hit.
[Graphical view ]
PIRSFi PIRSF001480. Mannose-6-phosphate_isomerase. 1 hit.
PRINTSi PR00714. MAN6PISMRASE.
SUPFAMi SSF51182. SSF51182. 1 hit.
TIGRFAMsi TIGR00218. manA. 1 hit.
PROSITEi PS00965. PMI_I_1. 1 hit.
PS00966. PMI_I_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "PMI40, an intron-containing gene required for early steps in yeast mannosylation."
    Smith D.J., Proudfoot A.E.I., Friedli L., Klig L.S., Paravicini G., Payton M.A.
    Mol. Cell. Biol. 12:2924-2930(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT SER-2.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Phosphomannose isomerase from Saccharomyces cerevisiae contains two inhibitory metal ion binding sites."
    Wells T.N.C., Coulin F., Payton M.A., Proudfoot A.E.I.
    Biochemistry 32:1294-1301(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: INHIBITION BY ZINC.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-107, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiMPI_YEAST
AccessioniPrimary (citable) accession number: P29952
Secondary accession number(s): D3DLP9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: January 23, 2007
Last modified: September 3, 2014
This is version 133 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 5220 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

External Data

Dasty 3

Similar proteinsi