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P29946 (CBIA_SALTY) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 102. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cobyrinic acid A,C-diamide synthase
Gene names
Name:cbiA
Ordered Locus Names:STM2035
OrganismSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) [Reference proteome] [HAMAP]
Taxonomic identifier99287 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella

Protein attributes

Sequence length459 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Responsible for the amidation of carboxylic groups at position A and C of either cobyrinic acid or hydrogenobrynic acid. NH2 groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation. HAMAP-Rule MF_00027

Pathway

Cofactor biosynthesis; adenosylcobalamin biosynthesis; cob(II)yrinate a,c-diamide from sirohydrochlorin (anaerobic route): step 10/10. HAMAP-Rule MF_00027

Sequence similarities

Belongs to the CobB/CobQ family. CobB subfamily.

Contains 1 GATase cobBQ-type domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 459459Cobyrinic acid A,C-diamide synthase HAMAP-Rule MF_00027
PRO_0000141269

Regions

Domain252 – 446195GATase cobBQ-type

Sites

Active site3341Nucleophile By similarity
Active site4381 By similarity

Experimental info

Sequence conflict361R → P in AAA27252. Ref.1
Sequence conflict1111M → I in AAA27252. Ref.1
Sequence conflict1281V → I in AAA27252. Ref.1
Sequence conflict1331A → T in AAA27252. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P29946 [UniParc].

Last modified January 23, 2002. Version 3.
Checksum: E1FEF1B5A37F5C14

FASTA45950,037
        10         20         30         40         50         60 
MAARHHAFIL AGTGSGCGKT TVTLGLLRLL QKRALRVQPF KVGPDYLDTG WHTAICGVAS 

        70         80         90        100        110        120 
RNLDSFMLPP PVLNALFCEQ MRQADIAVIE GVMGLYDGYG VDPNYCSTAA MAKQLGCPVI 

       130        140        150        160        170        180 
LLVDGKAVST SLAATVMGFQ HFDPTLNLAG VIVNRVTSDA HYQLLKNAIE HYCSLPVLGY 

       190        200        210        220        230        240 
VPPCDGVALP ERHLGLITAR ESLVNQQSWH DFAATLEQTV DVDALLSLSV LSALPAGMWP 

       250        260        270        280        290        300 
ERPDNTAGAG LTLALADDEA FNFYYPDNID LLERAGVNIV RFSPLHDRAL PDCQMIWLGG 

       310        320        330        340        350        360 
GYPELYAADL AANTVMLKHL RAAHQRGVAI YAECGGLMYL GSTLEDSGGE IHQMANIIPG 

       370        380        390        400        410        420 
HSKMGKRLTR FGYCEAQAMQ PTLLAAPGEI VRGHEFHYSD FIPETPAVMA CRKVRDGRVL 

       430        440        450 
QEWTGGWQTG NTFASYLHVH FAQRPEMLQH WLAAARRVL 

« Hide

References

« Hide 'large scale' references
[1]"Characterization of the cobalamin (vitamin B12) biosynthetic genes of Salmonella typhimurium."
Roth J.R., Lawrence J.G., Rubenfield M., Kieffer-Higgins S., Church G.M.
J. Bacteriol. 175:3303-3316(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: LT2.
[2]"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2."
McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. expand/collapse author list , Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K.
Nature 413:852-856(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: LT2 / SGSC1412 / ATCC 700720.
[3]"Cobalamin (vitamin B12) repression of the Cob operon in Salmonella typhimurium requires sequences within the leader and the first translated open reading frame."
Richter-Dahlfors A.A., Andersson D.I.
Mol. Microbiol. 6:743-749(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-106.
Strain: LT2.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L12006 Genomic DNA. Translation: AAA27252.1.
AE006468 Genomic DNA. Translation: AAL20939.1.
X63012 Genomic DNA. Translation: CAA44740.1.
PIRS20553.
RefSeqNP_460980.1. NC_003197.1.

3D structure databases

ProteinModelPortalP29946.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING99287.STM2035.

Proteomic databases

PaxDbP29946.
PRIDEP29946.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAL20939; AAL20939; STM2035.
GeneID1253556.
KEGGstm:STM2035.
PATRIC32382653. VBISalEnt20916_2157.

Phylogenomic databases

eggNOGCOG1797.
HOGENOMHOG000289958.
KOK02224.
OMAVATYMHM.
OrthoDBEOG6X6R8V.
PhylomeDBP29946.

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-13217.
SENT99287:GCTI-2047-MONOMER.
SABIO-RKP29946.
UniPathwayUPA00148; UER00231.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
3.40.50.880. 1 hit.
HAMAPMF_00027. CobB.
InterProIPR004484. CbiA_synth.
IPR029062. Class_I_gatase-like.
IPR017929. CobB/CobQ_GATase.
IPR002586. CobQ/CobB/MinD/ParA_Nub-bd_dom.
IPR011698. GATase_3.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamPF01656. CbiA. 1 hit.
PF07685. GATase_3. 1 hit.
[Graphical view]
SUPFAMSSF52317. SSF52317. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsTIGR00379. cobB. 1 hit.
PROSITEPS51274. GATASE_COBBQ. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCBIA_SALTY
AccessionPrimary (citable) accession number: P29946
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: January 23, 2002
Last modified: July 9, 2014
This is version 102 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways