P29946 (CBIA_SALTY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cobyrinic acid A,C-diamide synthase

Gene names

Name:	cbiA
Ordered Locus Names:	STM2035

Organism

Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) [Reference proteome] [HAMAP]

Taxonomic identifier

99287 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella › Salmonella choleraesuis › Salmonella enterica I › Salmonella typhimurium

Protein attributes

Sequence length	459 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Responsible for the amidation of carboxylic groups at position A and C of either cobyrinic acid or hydrogenobrynic acid. NH2 groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation. HAMAP-Rule MF_00027
Pathway	Cofactor biosynthesis; adenosylcobalamin biosynthesis; cob(II)yrinate a,c-diamide from sirohydrochlorin (anaerobic route): step 10/10. HAMAP-Rule MF_00027
Sequence similarities	Belongs to the CobB/CobQ family. CobB subfamily. Contains 1 GATase cobBQ-type domain.

Ontologies

Keywords
Biological process	Cobalamin biosynthesis
Domain	Glutamine amidotransferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cobalamin biosynthetic process Inferred from electronic annotation. Source: HAMAP glutamine metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	cobalamin-transporting ATPase activity Inferred from electronic annotation. Source: HAMAP cobyrinic acid a,c-diamide synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 459	459	Cobyrinic acid A,C-diamide synthase HAMAP-Rule MF_00027		PRO_0000141269
Regions
	Domain	252 – 446	195	GATase cobBQ-type
Sites
	Active site	334	1	Nucleophile By similarity
	Active site	438	1	By similarity
Experimental info
	Sequence conflict	36	1	R → P in AAA27252. Ref.1
	Sequence conflict	111	1	M → I in AAA27252. Ref.1
	Sequence conflict	128	1	V → I in AAA27252. Ref.1
	Sequence conflict	133	1	A → T in AAA27252. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P29946 [UniParc]. Last modified January 23, 2002. Version 3. Checksum: E1FEF1B5A37F5C14	FASTA	459	50,037
	10 20 30 40 50 60 MAARHHAFIL AGTGSGCGKT TVTLGLLRLL QKRALRVQPF KVGPDYLDTG WHTAICGVAS 70 80 90 100 110 120 RNLDSFMLPP PVLNALFCEQ MRQADIAVIE GVMGLYDGYG VDPNYCSTAA MAKQLGCPVI 130 140 150 160 170 180 LLVDGKAVST SLAATVMGFQ HFDPTLNLAG VIVNRVTSDA HYQLLKNAIE HYCSLPVLGY 190 200 210 220 230 240 VPPCDGVALP ERHLGLITAR ESLVNQQSWH DFAATLEQTV DVDALLSLSV LSALPAGMWP 250 260 270 280 290 300 ERPDNTAGAG LTLALADDEA FNFYYPDNID LLERAGVNIV RFSPLHDRAL PDCQMIWLGG 310 320 330 340 350 360 GYPELYAADL AANTVMLKHL RAAHQRGVAI YAECGGLMYL GSTLEDSGGE IHQMANIIPG 370 380 390 400 410 420 HSKMGKRLTR FGYCEAQAMQ PTLLAAPGEI VRGHEFHYSD FIPETPAVMA CRKVRDGRVL 430 440 450 QEWTGGWQTG NTFASYLHVH FAQRPEMLQH WLAAARRVL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of the cobalamin (vitamin B12) biosynthetic genes of Salmonella typhimurium." Roth J.R., Lawrence J.G., Rubenfield M., Kieffer-Higgins S., Church G.M. J. Bacteriol. 175:3303-3316(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: LT2.
[2]	"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2." McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. , Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K. Nature 413:852-856(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LT2 / SGSC1412 / ATCC 700720.
[3]	"Cobalamin (vitamin B12) repression of the Cob operon in Salmonella typhimurium requires sequences within the leader and the first translated open reading frame." Richter-Dahlfors A.A., Andersson D.I. Mol. Microbiol. 6:743-749(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-106. Strain: LT2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L12006 Genomic DNA. Translation: AAA27252.1. AE006468 Genomic DNA. Translation: AAL20939.1. X63012 Genomic DNA. Translation: CAA44740.1.
PIR	S20553.
RefSeq	NP_460980.1. NC_003197.1.
3D structure databases
ProteinModelPortal	P29946.
ModBase	Search...
Protein-protein interaction databases
STRING	99287.STM2035.
Proteomic databases
PaxDb	P29946.
PRIDE	P29946.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAL20939; AAL20939; STM2035.
GeneID	1253556.
KEGG	stm:STM2035.
PATRIC	32382653. VBISalEnt20916_2157.
Phylogenomic databases
eggNOG	COG1797.
HOGENOM	HOG000289958.
KO	K02224.
OMA	CWLPGGY.
ProtClustDB	PRK01077.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-13217. SENT99287:GCTI-2047-MONOMER.
SABIO-RK	P29946.
UniPathway	UPA00148; UER00231.
Family and domain databases
HAMAP	MF_00027. CobB.
InterPro	IPR004484. CbiA_synth. IPR017929. CobB/CobQ_GATase. IPR002586. CobQ/CobB/MinD/ParA_Nub-bd_dom. IPR011698. GATase_3. IPR027417. P-loop_NTPase. [Graphical view]
Pfam	PF01656. CbiA. 1 hit. PF07685. GATase_3. 1 hit. [Graphical view]
SUPFAM	SSF52540. SSF52540. 1 hit.
TIGRFAMs	TIGR00379. cobB. 1 hit.
PROSITE	PS51274. GATASE_COBBQ. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CBIA_SALTY

Accession

Primary (citable) accession number: P29946

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	January 23, 2002
Last modified:	May 29, 2013
This is version 95 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program