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P29946

- CBIA_SALTY

UniProt

P29946 - CBIA_SALTY

Protein

Cobyrinic acid A,C-diamide synthase

Gene

cbiA

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 103 (01 Oct 2014)
      Sequence version 3 (23 Jan 2002)
      Previous versions | rss
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    Functioni

    Responsible for the amidation of carboxylic groups at position A and C of either cobyrinic acid or hydrogenobrynic acid. NH2 groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei334 – 3341NucleophileBy similarity
    Active sitei438 – 4381By similarity

    GO - Molecular functioni

    1. cobalamin-transporting ATPase activity Source: UniProtKB-HAMAP
    2. cobyrinic acid a,c-diamide synthase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. cobalamin biosynthetic process Source: UniProtKB-HAMAP
    2. glutamine metabolic process Source: UniProtKB-HAMAP

    Keywords - Biological processi

    Cobalamin biosynthesis

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-13217.
    SENT99287:GCTI-2047-MONOMER.
    SABIO-RKP29946.
    UniPathwayiUPA00148; UER00231.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cobyrinic acid A,C-diamide synthase
    Gene namesi
    Name:cbiA
    Ordered Locus Names:STM2035
    OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
    Taxonomic identifieri99287 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella
    ProteomesiUP000001014: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 459459Cobyrinic acid A,C-diamide synthasePRO_0000141269Add
    BLAST

    Proteomic databases

    PaxDbiP29946.
    PRIDEiP29946.

    Interactioni

    Protein-protein interaction databases

    STRINGi99287.STM2035.

    Structurei

    3D structure databases

    ProteinModelPortaliP29946.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini252 – 446195GATase cobBQ-typeAdd
    BLAST

    Sequence similaritiesi

    Belongs to the CobB/CobQ family. CobB subfamily.Curated
    Contains 1 GATase cobBQ-type domain.Curated

    Keywords - Domaini

    Glutamine amidotransferase

    Phylogenomic databases

    eggNOGiCOG1797.
    HOGENOMiHOG000289958.
    KOiK02224.
    OMAiVATYMHM.
    OrthoDBiEOG6X6R8V.
    PhylomeDBiP29946.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    3.40.50.880. 1 hit.
    HAMAPiMF_00027. CobB.
    InterProiIPR004484. CbiA_synth.
    IPR029062. Class_I_gatase-like.
    IPR017929. CobB/CobQ_GATase.
    IPR002586. CobQ/CobB/MinD/ParA_Nub-bd_dom.
    IPR011698. GATase_3.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PfamiPF01656. CbiA. 1 hit.
    PF07685. GATase_3. 1 hit.
    [Graphical view]
    SUPFAMiSSF52317. SSF52317. 1 hit.
    SSF52540. SSF52540. 1 hit.
    TIGRFAMsiTIGR00379. cobB. 1 hit.
    PROSITEiPS51274. GATASE_COBBQ. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P29946-1 [UniParc]FASTAAdd to Basket

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    MAARHHAFIL AGTGSGCGKT TVTLGLLRLL QKRALRVQPF KVGPDYLDTG    50
    WHTAICGVAS RNLDSFMLPP PVLNALFCEQ MRQADIAVIE GVMGLYDGYG 100
    VDPNYCSTAA MAKQLGCPVI LLVDGKAVST SLAATVMGFQ HFDPTLNLAG 150
    VIVNRVTSDA HYQLLKNAIE HYCSLPVLGY VPPCDGVALP ERHLGLITAR 200
    ESLVNQQSWH DFAATLEQTV DVDALLSLSV LSALPAGMWP ERPDNTAGAG 250
    LTLALADDEA FNFYYPDNID LLERAGVNIV RFSPLHDRAL PDCQMIWLGG 300
    GYPELYAADL AANTVMLKHL RAAHQRGVAI YAECGGLMYL GSTLEDSGGE 350
    IHQMANIIPG HSKMGKRLTR FGYCEAQAMQ PTLLAAPGEI VRGHEFHYSD 400
    FIPETPAVMA CRKVRDGRVL QEWTGGWQTG NTFASYLHVH FAQRPEMLQH 450
    WLAAARRVL 459
    Length:459
    Mass (Da):50,037
    Last modified:January 23, 2002 - v3
    Checksum:iE1FEF1B5A37F5C14
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti36 – 361R → P in AAA27252. (PubMed:8501034)Curated
    Sequence conflicti111 – 1111M → I in AAA27252. (PubMed:8501034)Curated
    Sequence conflicti128 – 1281V → I in AAA27252. (PubMed:8501034)Curated
    Sequence conflicti133 – 1331A → T in AAA27252. (PubMed:8501034)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L12006 Genomic DNA. Translation: AAA27252.1.
    AE006468 Genomic DNA. Translation: AAL20939.1.
    X63012 Genomic DNA. Translation: CAA44740.1.
    PIRiS20553.
    RefSeqiNP_460980.1. NC_003197.1.

    Genome annotation databases

    EnsemblBacteriaiAAL20939; AAL20939; STM2035.
    GeneIDi1253556.
    KEGGistm:STM2035.
    PATRICi32382653. VBISalEnt20916_2157.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L12006 Genomic DNA. Translation: AAA27252.1 .
    AE006468 Genomic DNA. Translation: AAL20939.1 .
    X63012 Genomic DNA. Translation: CAA44740.1 .
    PIRi S20553.
    RefSeqi NP_460980.1. NC_003197.1.

    3D structure databases

    ProteinModelPortali P29946.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 99287.STM2035.

    Proteomic databases

    PaxDbi P29946.
    PRIDEi P29946.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAL20939 ; AAL20939 ; STM2035 .
    GeneIDi 1253556.
    KEGGi stm:STM2035.
    PATRICi 32382653. VBISalEnt20916_2157.

    Phylogenomic databases

    eggNOGi COG1797.
    HOGENOMi HOG000289958.
    KOi K02224.
    OMAi VATYMHM.
    OrthoDBi EOG6X6R8V.
    PhylomeDBi P29946.

    Enzyme and pathway databases

    UniPathwayi UPA00148 ; UER00231 .
    BioCyci MetaCyc:MONOMER-13217.
    SENT99287:GCTI-2047-MONOMER.
    SABIO-RK P29946.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    3.40.50.880. 1 hit.
    HAMAPi MF_00027. CobB.
    InterProi IPR004484. CbiA_synth.
    IPR029062. Class_I_gatase-like.
    IPR017929. CobB/CobQ_GATase.
    IPR002586. CobQ/CobB/MinD/ParA_Nub-bd_dom.
    IPR011698. GATase_3.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    Pfami PF01656. CbiA. 1 hit.
    PF07685. GATase_3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52317. SSF52317. 1 hit.
    SSF52540. SSF52540. 1 hit.
    TIGRFAMsi TIGR00379. cobB. 1 hit.
    PROSITEi PS51274. GATASE_COBBQ. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of the cobalamin (vitamin B12) biosynthetic genes of Salmonella typhimurium."
      Roth J.R., Lawrence J.G., Rubenfield M., Kieffer-Higgins S., Church G.M.
      J. Bacteriol. 175:3303-3316(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: LT2.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: LT2 / SGSC1412 / ATCC 700720.
    3. "Cobalamin (vitamin B12) repression of the Cob operon in Salmonella typhimurium requires sequences within the leader and the first translated open reading frame."
      Richter-Dahlfors A.A., Andersson D.I.
      Mol. Microbiol. 6:743-749(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-106.
      Strain: LT2.

    Entry informationi

    Entry nameiCBIA_SALTY
    AccessioniPrimary (citable) accession number: P29946
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1993
    Last sequence update: January 23, 2002
    Last modified: October 1, 2014
    This is version 103 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3