P29945 (KDGL_PSEDE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Diacylglycerol kinase
Short name=DAGK
EC=2.7.1.107

Alternative name(s):
Diglyceride kinase
Short name=DGK

Gene names

Name:

dgkA

Organism

Pseudomonas denitrificans

Taxonomic identifier

43306 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	137 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Recycling of diacylglycerol produced during the turnover of membrane phospholipid By similarity.
Catalytic activity	ATP + 1,2-diacylglycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the bacterial diacylglycerol kinase family.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Gene Ontology (GO)
Biological process	phospholipid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW diacylglycerol kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 137	137	Diacylglycerol kinase		PRO_0000195266
Regions
	Topological domain	1 – 46	46	Cytoplasmic Potential
	Transmembrane	47 – 62	16	Helical; Potential
	Transmembrane	66 – 82	17	Helical; Potential
	Topological domain	83 – 109	27	Cytoplasmic Potential
	Transmembrane	110 – 133	24	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	P29945 [UniParc]. Last modified April 1, 1993. Version 1. Checksum: 796675BA2D2C773F	FASTA	137	14,802
	10 20 30 40 50 60 MDAKNTTHRI GQTGPVEKQT GIRHLFAAAS YSLGGAKRLI GEAAFRHELI AFAAAMIAFI 70 80 90 100 110 120 IVGATFFQYV AMAILFLLMM AFEAINTAIE EIVDRVSPEI SEMGKNAKDL GSFACLCLIV 130 ANGVYAAYVV IFDGFMN

References

[1]

"Genetic analysis, nucleotide sequence, and products of two Pseudomonas denitrificans cob genes encoding nicotinate-nucleotide: dimethylbenzimidazole phosphoribosyltransferase and cobalamin (5'-phosphate) synthase." Cameron B., Blanche F., Rouyez M.-C., Bisch D., Famechon A., Couder M., Cauchois L., Thibaut D., Debussche L., Crouzet J. J. Bacteriol. 173:6066-6073(1991) [PubMed: 1917841] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	M62868 Genomic DNA. Translation: AAA25789.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR000829. Diacylglycerol_kinase_prok. [Graphical view]
Pfam	PF01219. DAGK_prokar. 1 hit. [Graphical view]
PROSITE	PS01069. DAGK_PROKAR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KDGL_PSEDE

Accession

Primary (citable) accession number: P29945

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	May 31, 2011
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program