P29936 (COBV_PSEDE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cobalamin synthase
EC=2.-.-.-

Gene names

Name:

cobV

Organism

Pseudomonas denitrificans

Taxonomic identifier

43306 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas pertucinogena group

Protein attributes

Sequence length	262 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin). Also synthesizes Ado-cobalamin 5'-phosphate from Ado-cobinamide-GDP and alpha-ribazole 5'-phosphate. Ref.1
Catalytic activity	GDP-cobinamide + alpha-ribazole 5'-phosphate = cobalamin 5'-phosphate + GMP. HAMAP-Rule MF_00719 GDP-cobinamide + alpha-ribazole = cobalamin + GMP. HAMAP-Rule MF_00719
Pathway	Cofactor biosynthesis; adenosylcobalamin biosynthesis; adenosylcobalamin from cob(II)yrinate a,c-diamide: step 7/7. HAMAP-Rule MF_00719
Subunit structure	Associated with a large complex of proteins.
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity HAMAP-Rule MF_00719.
Sequence similarities	Belongs to the CobS family.
Sequence caution	The sequence AAA25787.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Cobalamin biosynthesis
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Transferase
Gene Ontology (GO)
Biological_process	cobalamin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	cobalamin 5'-phosphate synthase activity Inferred from electronic annotation. Source: InterPro cobalamin-transporting ATPase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 262	262	Cobalamin synthase HAMAP-Rule MF_00719		PRO_0000146888
Regions
	Transmembrane	41 – 63	23	Helical; Potential
	Transmembrane	68 – 85	18	Helical; Potential
	Transmembrane	115 – 134	20	Helical; Potential
	Transmembrane	141 – 163	23	Helical; Potential
	Transmembrane	201 – 221	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	P29936 [UniParc]. Last modified October 10, 2003. Version 2. Checksum: 2E2E833C94518D5F	FASTA	262	26,842
	10 20 30 40 50 60 MGFVGDFCDD VARSIGFLSR IPMPARHFEG YDGRLSRAVR AFPFAGLAIA LPSAAVAMAL 70 80 90 100 110 120 MALQVSSLFA AFVVVAIQAL VTGALHEDGL GDTADGFGGG RDREAALAIM KDSRIGTYAA 130 140 150 160 170 180 VALILSFGLR VSAFASILPL FSPLGAAMAI LGAACLSRAA MVWHWSSLPP ARSSGVAASA 190 200 210 220 230 240 GEPEPAATRF ALAFGLLVAM LLFYLAQVPA LGVIAALVAF LATVKGFARL AMRKIGGQTG 250 260 DTIGATQQLT EIAVLGALAL TV

References

[1]

"Genetic analysis, nucleotide sequence, and products of two Pseudomonas denitrificans cob genes encoding nicotinate-nucleotide: dimethylbenzimidazole phosphoribosyltransferase and cobalamin (5'-phosphate) synthase." Cameron B., Blanche F., Rouyez M.-C., Bisch D., Famechon A., Couder M., Cauchois L., Thibaut D., Debussche L., Crouzet J. J. Bacteriol. 173:6066-6073(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M62868 Genomic DNA. Translation: AAA25787.1. Different initiation.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-144.
UniPathway	UPA00148; UER00238.
Family and domain databases
HAMAP	MF_00719. CobS.
InterPro	IPR003805. Cbl_biosynth_CobS. [Graphical view]
Pfam	PF02654. CobS. 1 hit. [Graphical view]
TIGRFAMs	TIGR00317. cobS. 1 hit.
ProtoNet	Search...

Entry information

Entry name

COBV_PSEDE

Accession

Primary (citable) accession number: P29936

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	October 10, 2003
Last modified:	April 3, 2013
This is version 61 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program