Reviewed, UniProtKB/Swiss-Prot P29931 (COBP_PSEDE)

Names and origin

Protein names

Recommended name:
    Bifunctional adenosylcobalamin biosynthesis protein cobP
Including the following 2 domains:
    1- Recommended name:
            Adenosylcobinamide kinase
              EC=2.7.1.156
    2- Recommended name:
            Adenosylcobinamide-phosphate guanylyltransferase
              EC=2.7.7.62

Gene names

Name:

cobP

Organism

Pseudomonas denitrificans

Taxonomic identifier

43306 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	174 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes ATP-dependent phosphorylation of adenosylcobinamide and addition of GMP to adenosylcobinamide phosphate.
Catalytic activity	ATP or GTP + adenosylcobinamide = adenosylcobinamide phosphate + ADP or GDP. GTP + adenosylcobinamide phosphate = diphosphate + adenosylcobinamide-GDP.
Pathway	Cofactor biosynthesis; adenosylcobalamin biosynthesis; adenosylcobalamin from cob(II)yrinic acid a,c-diamide: step 5/7. Cofactor biosynthesis; adenosylcobalamin biosynthesis; adenosylcobalamin from cob(II)yrinic acid a,c-diamide: step 6/7.
Subunit structure	Homodimer.

Ontologies

Keywords
Biological process	Cobalamin biosynthesis
Ligand	ATP-binding GTP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Direct protein sequencing Multifunctional enzyme
Gene Ontology (GO)
Biological process	cobalamin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW GTP binding Inferred from electronic annotation. Source: UniProtKB-KW adenosylcobinamide kinase activity Inferred from electronic annotation. Source: EC adenosylcobinamide-phosphate guanylyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.2
	Chain	2 – 174	173	Bifunctional adenosylcobalamin biosynthesis protein cobP		PRO_0000089993
Regions
	Nucleotide binding	13 – 20	8	ATP Potential
Sites
	Active site	54	1	GMP-histidine intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P29931-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 71580200BC9DC4DC	FASTA	174	19,442
	10 20 30 40 50 60 MSSLSAGPVL VLGGARSGKS SFSERLVEAS GFTMHYVATG RAWDDEMRER IDHHRTRRGE 70 80 90 100 110 120 GWTTHEEPLD LVGILRRIDD PSHVVLIDCL TLWVTNLMLE ERDMTAEFAA LVAYLPEARA 130 140 150 160 170 RLVFVSNEVG LGIVPENRMA REFRDHAGRL HQIVAEKSAE VYFVAAGLPL KMKG

References

[1]

"Nucleotide sequence and genetic analysis of a 13.1-kilobase-pair Pseudomonas denitrificans DNA fragment containing five cob genes and identification of structural genes encoding Cob(I)alamin adenosyltransferase, cobyric acid synthase, and bifunctional cobinamide kinase-cobinamide phosphate guanylyltransferase." Crouzet J., Levy-Schil S., Cameron B., Cauchois L., Rigault S., Rouyez M.-C., Blanche F., Debussche L., Thibaut D. J. Bacteriol. 173:6074-6087(1991) [PubMed: 1655697] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

[2]

"A bifunctional protein from Pseudomonas denitrificans carries cobinamide kinase and cobinamide phosphate guanylyltransferase activities." Blanche F., Debussche L., Famechon A., Thibaut D., Cameron B., Crouzet J. J. Bacteriol. 173:6052-6057(1991) [PubMed: 1655696] [Abstract] Cited for: CHARACTERIZATION, PROTEIN SEQUENCE OF 2-11.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M62866 Genomic DNA. Translation: AAA25778.1.
3D structure databases
HSSP	HSSP built from PDB template 1CBU based on UniProtKB Q05599.
ModBase	Search...
Enzyme and pathway databases
BioCyc	MetaCyc:MON-143.
BRENDA	2.7.1.156. 1952. 2.7.7.62. 1952.
Family and domain databases
InterPro	IPR003203. Cobinamide_kinase/P_G-Trfase. [Graphical view]
Pfam	PF02283. CobU. 1 hit. [Graphical view]
PIRSF	PIRSF006135. CobU. 1 hit.
ProDom	PD011091. CobU. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

COBP_PSEDE

Accession

Primary (citable) accession number: P29931

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 51 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)