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P29930

- COBO_PSEDE

UniProt

P29930 - COBO_PSEDE

Protein

Cob(I)yrinic acid a,c-diamide adenosyltransferase

Gene

cobO

Organism
Pseudomonas denitrificans
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 71 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    • Comment

    Functioni

    Required for both de novo synthesis of the corrin ring for the assimilation of exogenous corrinoids. Participates in the adenosylation of a variety of incomplete and complete corrinoids By similarity.By similarity

    Catalytic activityi

    ATP + cob(I)yrinic acid a,c-diamide = triphosphate + adenosylcob(III)yrinic acid a,c-diamide.
    ATP + cobinamide = triphosphate + adenosylcobinamide.

    Cofactori

    Manganese.

    Pathwayi

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi50 – 567ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. cob(I)yrinic acid a,c-diamide adenosyltransferase activity Source: UniProtKB-EC

    GO - Biological processi

    1. cobalamin biosynthetic process Source: UniProtKB-UniPathway
    2. porphyrin-containing compound biosynthetic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Cobalamin biosynthesis, Porphyrin biosynthesis

    Keywords - Ligandi

    ATP-binding, Manganese, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-123.
    RETL1328306-WGS:GSTH-2511-MONOMER.
    BRENDAi2.5.1.17. 5114.
    UniPathwayiUPA00148; UER00233.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cob(I)yrinic acid a,c-diamide adenosyltransferase (EC:2.5.1.17)
    Alternative name(s):
    Cob(I)alamin adenosyltransferase
    Corrinoid adenosyltransferase
    Gene namesi
    Name:cobO
    OrganismiPseudomonas denitrificans
    Taxonomic identifieri43306 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 214213Cob(I)yrinic acid a,c-diamide adenosyltransferasePRO_0000089992Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.

    Structurei

    3D structure databases

    ProteinModelPortaliP29930.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR003724. AdoCbl_synth_CblAdoTrfase_CobA.
    IPR025826. Co_AT_N_dom.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PfamiPF12557. Co_AT_N. 1 hit.
    PF02572. CobA_CobO_BtuR. 1 hit.
    [Graphical view]
    PIRSFiPIRSF015617. Adensltrnsf_CobA. 1 hit.
    SUPFAMiSSF52540. SSF52540. 1 hit.
    TIGRFAMsiTIGR00708. cobA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P29930-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSDETTVGGE APAEKDDARH AMKMAKKKAA REKIMATKTD EKGLIIVNTG    50
    KGKGKSTAGF GMIFRHIAHG MPCAVVQFIK GAMATGEREL IEKHFGDVCQ 100
    FYTLGEGFTW ETQDRARDVA MAEKAWEKAK ELIRDERNSM VLLDEINIAL 150
    RYDYIDVAEV VRFLKEEKPH MTHVVLTGRN AKEDLIEVAD LVTEMELIKH 200
    PFRSGIKAQQ GVEF 214
    Length:214
    Mass (Da):24,028
    Last modified:January 23, 2007 - v3
    Checksum:iA110C89668990ECC
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti14 – 141E → K AA sequence (PubMed:1917862)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M62866 Genomic DNA. Translation: AAA25781.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M62866 Genomic DNA. Translation: AAA25781.1 .

    3D structure databases

    ProteinModelPortali P29930.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00148 ; UER00233 .
    BioCyci MetaCyc:MONOMER-123.
    RETL1328306-WGS:GSTH-2511-MONOMER.
    BRENDAi 2.5.1.17. 5114.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR003724. AdoCbl_synth_CblAdoTrfase_CobA.
    IPR025826. Co_AT_N_dom.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    Pfami PF12557. Co_AT_N. 1 hit.
    PF02572. CobA_CobO_BtuR. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF015617. Adensltrnsf_CobA. 1 hit.
    SUPFAMi SSF52540. SSF52540. 1 hit.
    TIGRFAMsi TIGR00708. cobA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence and genetic analysis of a 13.1-kilobase-pair Pseudomonas denitrificans DNA fragment containing five cob genes and identification of structural genes encoding Cob(I)alamin adenosyltransferase, cobyric acid synthase, and bifunctional cobinamide kinase-cobinamide phosphate guanylyltransferase."
      Crouzet J., Levy-Schil S., Cameron B., Cauchois L., Rigault S., Rouyez M.-C., Blanche F., Debussche L., Thibaut D.
      J. Bacteriol. 173:6074-6087(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Purification and partial characterization of Cob(I)alamin adenosyltransferase from Pseudomonas denitrificans."
      Debussche L., Couder M., Thibaut D., Cameron B., Crouzet J., Blanche F.
      J. Bacteriol. 173:6300-6302(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION, PROTEIN SEQUENCE OF 2-15.

    Entry informationi

    Entry nameiCOBO_PSEDE
    AccessioniPrimary (citable) accession number: P29930
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1993
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 71 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3