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P29928

- SUMT_BACME

UniProt

P29928 - SUMT_BACME

Protein

Uroporphyrinogen-III C-methyltransferase

Gene

cobA

Organism
Bacillus megaterium
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 72 (01 Oct 2014)
      Sequence version 1 (01 Apr 1993)
      Previous versions | rss
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    Functioni

    Catalyzes both methylations at C-2 and C-7 of uroporphyrinogen III leading to precorrin-1 and precorrin-2; their oxidative esterification gives respectively factor I octamethyl ester and sirohydrochlorin.

    Catalytic activityi

    S-adenosyl-L-methionine + uroporphyrinogen III = S-adenosyl-L-homocysteine + precorrin-1.
    S-adenosyl-L-methionine + precorrin-1 = S-adenosyl-L-homocysteine + precorrin-2.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei11 – 111S-adenosyl-L-methionine; via carbonyl oxygenBy similarity
    Binding sitei117 – 1171S-adenosyl-L-methionineBy similarity
    Binding sitei170 – 1701S-adenosyl-L-methionine; via amide nitrogenBy similarity

    GO - Molecular functioni

    1. precorrin-2 dehydrogenase activity Source: InterPro
    2. uroporphyrin-III C-methyltransferase activity Source: UniProtKB-EC

    GO - Biological processi

    1. cobalamin biosynthetic process Source: UniProtKB-UniPathway
    2. siroheme biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    Cobalamin biosynthesis, Porphyrin biosynthesis

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Enzyme and pathway databases

    UniPathwayiUPA00148; UER00211.
    UPA00262; UER00211.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Uroporphyrinogen-III C-methyltransferase (EC:2.1.1.107)
    Short name:
    Urogen III methylase
    Alternative name(s):
    SUMT
    Uroporphyrinogen III methylase
    Short name:
    UROM
    Gene namesi
    Name:cobA
    OrganismiBacillus megaterium
    Taxonomic identifieri1404 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 238238Uroporphyrinogen-III C-methyltransferasePRO_0000150369Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.Curated

    Structurei

    3D structure databases

    ProteinModelPortaliP29928.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni87 – 893S-adenosyl-L-methionine bindingBy similarity

    Sequence similaritiesi

    Belongs to the precorrin methyltransferase family.Curated

    Family and domain databases

    Gene3Di3.30.950.10. 1 hit.
    3.40.1010.10. 1 hit.
    InterProiIPR000878. 4pyrrol_Mease.
    IPR014777. 4pyrrole_Mease_sub1.
    IPR014776. 4pyrrole_Mease_sub2.
    IPR006366. CobA/CysG_C.
    IPR003043. Uropor_MeTrfase_CS.
    [Graphical view]
    PfamiPF00590. TP_methylase. 1 hit.
    [Graphical view]
    SUPFAMiSSF53790. SSF53790. 1 hit.
    TIGRFAMsiTIGR01469. cobA_cysG_Cterm. 1 hit.
    PROSITEiPS00839. SUMT_1. 1 hit.
    PS00840. SUMT_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P29928-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGKVYLVGAG PGDPDLITLK GLKAIQQADV ILYDRLVNKD LLEYAKSDAD    50
    IIYCGKLPNY HTLKQETINN FLVKFAKKGK IVTRLKGGDP FVFGRGGEEA 100
    EALVQQGISF EIVPGITSGI AAAAYAGIPV THREYSASFA FVAGHRKDSK 150
    HDAIKWDSLA KGVDTLAIYM GVRNLPYICQ QLMKHGKTSA TPIALIHWGT 200
    CADQRTVTGT LGTIVDIVKE EQIENPSMII VGEVVNFS 238
    Length:238
    Mass (Da):25,822
    Last modified:April 1, 1993 - v1
    Checksum:i97993B56C894F307
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M62881 Genomic DNA. Translation: AAA22317.1.
    PIRiA42479.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M62881 Genomic DNA. Translation: AAA22317.1 .
    PIRi A42479.

    3D structure databases

    ProteinModelPortali P29928.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00148 ; UER00211 .
    UPA00262 ; UER00211 .

    Family and domain databases

    Gene3Di 3.30.950.10. 1 hit.
    3.40.1010.10. 1 hit.
    InterProi IPR000878. 4pyrrol_Mease.
    IPR014777. 4pyrrole_Mease_sub1.
    IPR014776. 4pyrrole_Mease_sub2.
    IPR006366. CobA/CysG_C.
    IPR003043. Uropor_MeTrfase_CS.
    [Graphical view ]
    Pfami PF00590. TP_methylase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53790. SSF53790. 1 hit.
    TIGRFAMsi TIGR01469. cobA_cysG_Cterm. 1 hit.
    PROSITEi PS00839. SUMT_1. 1 hit.
    PS00840. SUMT_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Primary structure, expression in Escherichia coli, and properties of S-adenosyl-L-methionine:uroporphyrinogen III methyltransferase from Bacillus megaterium."
      Robin C., Blanche F., Cauchois L., Cameron B., Couder M., Crouzet J.
      J. Bacteriol. 173:4893-4896(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 10778 / DSM 2894 / NCIMB 8508 / NRRL B-938.

    Entry informationi

    Entry nameiSUMT_BACME
    AccessioniPrimary (citable) accession number: P29928
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1993
    Last sequence update: April 1, 1993
    Last modified: October 1, 2014
    This is version 72 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3