Reviewed, UniProtKB/Swiss-Prot P29926 (NQO14_PARDE)

Names and origin

Protein names

Recommended name:
    NADH-quinone oxidoreductase chain 14
    EC=1.6.99.5
Alternative name(s):
    NADH dehydrogenase I, chain 14
    NDH-1, chain 14

Gene names

Name:

nqo14

Organism

Paracoccus denitrificans

Taxonomic identifier

266 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Paracoccus

Protein attributes

Sequence length	499 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.
Catalytic activity	NADH + quinone = NAD+ + quinol.
Subunit structure	NDH-1 is composed of at least 14 different subunits, nqo1 to nqo14. The complex has a L-shaped structure, with the hydrophobic arm (subunits nqo7, nqo8, nqo10 to nqo14) embedded in the inner membrane and the hydrophilic peripheral arm (subunits nqo1 to nqo6, nqo9) protruding into the bacterial cytoplasm. The hydrophilic domain contains all the redox centers.
Subcellular location	Cell inner membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the complex I subunit 2 family.

Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Ligand	NAD Ubiquinone
Molecular function	Oxidoreductase
PTM	Quinone
Gene Ontology (GO)
Biological process	ATP synthesis coupled electron transport Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 499	499	NADH-quinone oxidoreductase chain 14		PRO_0000117686
Regions
	Transmembrane	9 – 29	21	Potential
	Transmembrane	37 – 57	21	Potential
	Transmembrane	76 – 96	21	Potential
	Transmembrane	104 – 124	21	Potential
	Transmembrane	126 – 146	21	Potential
	Transmembrane	161 – 181	21	Potential
	Transmembrane	196 – 216	21	Potential
	Transmembrane	235 – 255	21	Potential
	Transmembrane	269 – 289	21	Potential
	Transmembrane	301 – 321	21	Potential
	Transmembrane	325 – 345	21	Potential
	Transmembrane	369 – 389	21	Potential
	Transmembrane	402 – 422	21	Potential
	Transmembrane	446 – 466	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	P29926-1 [UniParc]. Last modified April 1, 1993. Version 1. Checksum: F116A0CEA09ED72A	FASTA	499	52,535
	10 20 30 40 50 60 MTSLDFSTIL PEVVLAGYAL AALMAGAYLG KDRLARTLLW VTVAAFLVVA AMVGLGNHVD 70 80 90 100 110 120 GAAFHGMFID DGFSRFAKVV TLVAAAGVLA MSADYMQRRN MLRFEFPIIV ALAVLGMMFM 130 140 150 160 170 180 VSAGDLLTLY MGLELQSLAL YVVAAMRRDS VRSSEAGLKY FVLGSLSSGL LLYGASLVYG 190 200 210 220 230 240 FAGTTGFEGI ISTIEAGHLS LGVLFGLVFM LVGLSFKVSA VPFHMWTPDV YEGSPTPVTA 250 260 270 280 290 300 FFATAPKVAA MALIARLVFD AFGHVIGDWS QIVAALAVMS MFLGSIAGIG QTNIKRLMAY 310 320 330 340 350 360 SSIAHMGFAL VGLAAGTAIG VQNMLLYMTI YAVMNIGTFA FILSMERDGV PVTDLAALNR 370 380 390 400 410 420 FAWTDPVKAL AMLVLMFSLA GVPPTLGFFA KFGVLTAAVD AGMGWLAVLG VIASVIGAFY 430 440 450 460 470 480 YLRIVYYMYF GGESEGMTSR MGAVQYLALM VPALAMLVGA ISMFGVDSAA GRAAETLVGP 490 VAAIEQPAEA AQAEPVQGE

References

[1]

"DNA sequencing of the seven remaining structural genes of the gene cluster encoding the energy-transducing NADH-quinone oxidoreductase of Paracoccus denitrificans." Xu X., Matsuno-Yagi A., Yagi T. Biochemistry 32:968-981(1993) [PubMed: 8422400] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 13543 / NRRL B-3784.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L02354 Genomic DNA. Translation: AAA25600.1.
PIR	A47751.
3D structure databases
ModBase	Search...
Protein family/group databases
TCDB	3.D.1.2.1. proton-translocating NADH dehydrogenase (NDH) family.
Enzyme and pathway databases
BRENDA	1.6.99.5. 59.
Family and domain databases
InterPro	IPR010096. NADH-Q_OxRdtase_chainN. IPR001750. NADH_UbQ/plastoQ_OxRdtase. IPR003916. NADH_UbQ_OxRdtase_chain5. [Graphical view]
Pfam	PF00361. Oxidored_q1. 1 hit. [Graphical view]
PRINTS	PR01434. NADHDHGNASE5.
TIGRFAMs	TIGR01770. NDH_I_N. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NQO14_PARDE

Accession

Primary (citable) accession number: P29926

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	June 16, 2009
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)