Reviewed, UniProtKB/Swiss-Prot P29925 (NQO13_PARDE)

Names and origin

Protein names

Recommended name:
    NADH-quinone oxidoreductase chain 13
    EC=1.6.99.5
Alternative name(s):
    NADH dehydrogenase I, chain 13
    NDH-1, chain 13

Gene names

Name:

nqo13

Organism

Paracoccus denitrificans

Taxonomic identifier

266 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Paracoccus

Protein attributes

Sequence length	513 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.
Catalytic activity	NADH + quinone = NAD+ + quinol.
Subunit structure	NDH-1 is composed of at least 14 different subunits, nqo1 to nqo14. The complex has a L-shaped structure, with the hydrophobic arm (subunits nqo7, nqo8, nqo10 to nqo14) embedded in the inner membrane and the hydrophilic peripheral arm (subunits nqo1 to nqo6, nqo9) protruding into the bacterial cytoplasm. The hydrophilic domain contains all the redox centers.
Subcellular location	Cell inner membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the complex I subunit 4 family.

Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Ligand	NAD Ubiquinone
Molecular function	Oxidoreductase
PTM	Quinone
Gene Ontology (GO)
Biological process	ATP synthesis coupled electron transport Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 513	513	NADH-quinone oxidoreductase chain 13		PRO_0000118037
Regions
	Transmembrane	3 – 23	21	Potential
	Transmembrane	34 – 54	21	Potential
	Transmembrane	81 – 101	21	Potential
	Transmembrane	112 – 132	21	Potential
	Transmembrane	133 – 153	21	Potential
	Transmembrane	164 – 184	21	Potential
	Transmembrane	211 – 231	21	Potential
	Transmembrane	250 – 270	21	Potential
	Transmembrane	277 – 297	21	Potential
	Transmembrane	312 – 332	21	Potential
	Transmembrane	340 – 360	21	Potential
	Transmembrane	383 – 403	21	Potential
	Transmembrane	418 – 438	21	Potential
	Transmembrane	463 – 483	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	P29925-1 [UniParc]. Last modified April 1, 1993. Version 1. Checksum: 8164DEACA20FE739	FASTA	513	56,417
	10 20 30 40 50 60 MTNLLSIITF LPIVAAIIMA LFLRGQDEAA ARNAKWLALL TTTATFVISL FVLFRFDPAN 70 80 90 100 110 120 TGFQFVEDHA WIMGVCYKMG VDGISVLFVL LTTFMMPLTI LSTWQVQDKV KEYMIAFLVL 130 140 150 160 170 180 EGLMIGVFTA LDLVLFYLFF EAGLIPMFLI IGIWGGKDRI YASFKFFLYT FLGSVLMLVA 190 200 210 220 230 240 MIAMYRMAGT TDIPTLLTFD FPSENFRLLG MTVVGGMQML LFLAFFASFA VKMPMWPVHT 250 260 270 280 290 300 WLPDAHVQAP TAGSVLLAAV LLKMGGYGFL RFSLPMFPVA SGVAQPYVFW LSAIAIVYTS 310 320 330 340 350 360 LVALAQSDMK KVIAYSSVAH MGYVTMGVFA ANQIGVDGAI FQMLSHGFIS GALFLCVGVI 370 380 390 400 410 420 YDRMHTREID AYGGLVNRMP AYAAVFMFFT MANVGLPGTS GFVGEFLTLM GVFRVDTWVA 430 440 450 460 470 480 LVATSGVILS AAYALWLYRR VTLGQLIKES LKSITDMTPR ERWVFIPLIA MTLILGVYPR 490 500 510 LVTDVTGPAV AALVQDYNQS QPAAPVATAQ ASH

References

[1]

"DNA sequencing of the seven remaining structural genes of the gene cluster encoding the energy-transducing NADH-quinone oxidoreductase of Paracoccus denitrificans." Xu X., Matsuno-Yagi A., Yagi T. Biochemistry 32:968-981(1993) [PubMed: 8422400] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 13543 / NRRL B-3784.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L02354 Genomic DNA. Translation: AAA25599.1.
PIR	I45456.
3D structure databases
ModBase	Search...
Protein family/group databases
TCDB	3.D.1.2.1. proton-translocating NADH dehydrogenase (NDH) family.
Enzyme and pathway databases
BRENDA	1.6.99.5. 59.
Family and domain databases
InterPro	IPR010227. NADH_Q_OxRdtase_chainM. IPR001750. NADH_UbQ/plastoQ_OxRdtase. IPR003918. NADH_UbQ_OxRdtase_chain4. [Graphical view]
Pfam	PF00361. Oxidored_q1. 1 hit. [Graphical view]
PRINTS	PR01437. NUOXDRDTASE4.
TIGRFAMs	TIGR01972. NDH_I_M. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NQO13_PARDE

Accession

Primary (citable) accession number: P29925

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	June 16, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)