Reviewed, UniProtKB/Swiss-Prot P29924 (NQO12_PARDE)

Names and origin

Protein names

Recommended name:
    NADH-quinone oxidoreductase chain 12
    EC=1.6.99.5
Alternative name(s):
    NADH dehydrogenase I, chain 12
    NDH-1, chain 12

Gene names

Name:

nqo12

Organism

Paracoccus denitrificans

Taxonomic identifier

266 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Paracoccus

Protein attributes

Sequence length	703 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.
Catalytic activity	NADH + quinone = NAD+ + quinol.
Subunit structure	NDH-1 is composed of at least 14 different subunits, nqo1 to nqo14. The complex has a L-shaped structure, with the hydrophobic arm (subunits nqo7, nqo8, nqo10 to nqo14) embedded in the inner membrane and the hydrophilic peripheral arm (subunits nqo1 to nqo6, nqo9) protruding into the bacterial cytoplasm. The hydrophilic domain contains all the redox centers.
Subcellular location	Cell inner membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the complex I subunit 5 family.

Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Ligand	NAD Ubiquinone
Molecular function	Oxidoreductase
PTM	Quinone
Gene Ontology (GO)
Biological process	ATP synthesis coupled electron transport Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 703	703	NADH-quinone oxidoreductase chain 12		PRO_0000118211
Regions
	Transmembrane	4 – 24	21	Potential
	Transmembrane	30 – 50	21	Potential
	Transmembrane	79 – 99	21	Potential
	Transmembrane	116 – 136	21	Potential
	Transmembrane	138 – 158	21	Potential
	Transmembrane	179 – 199	21	Potential
	Transmembrane	224 – 244	21	Potential
	Transmembrane	256 – 276	21	Potential
	Transmembrane	290 – 310	21	Potential
	Transmembrane	325 – 345	21	Potential
	Transmembrane	346 – 366	21	Potential
	Transmembrane	381 – 401	21	Potential
	Transmembrane	415 – 435	21	Potential
	Transmembrane	475 – 495	21	Potential
	Transmembrane	580 – 600	21	Potential
	Transmembrane	679 – 699	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	P29924-1 [UniParc]. Last modified April 1, 1993. Version 1. Checksum: 433993D4081B14CA	FASTA	703	77,706
	10 20 30 40 50 60 MEKFVLFAPL IASLIAGLGW RAIGEKAAQY LTTGVLFLSC LISWYLFLSF DGVPRHIPVL 70 80 90 100 110 120 DWVVTGDFHA EWAIRLDRLT AIMLIVVTTV SALVHMYSLG YMAHDDNWTH DEHYKARFFA 130 140 150 160 170 180 YLSFFTFAML MLVTADNLLQ MFFGWEGVGV ASYLLIGFYY KKASANAAAM KAFIVNRVGD 190 200 210 220 230 240 FGFLLGIFGI YWLTGSVQFD EIFRQVPQLA QTEIDFLWRD WNAANLLGFL LFVGAMGKSA 250 260 270 280 290 300 QLLLHTWLPD AMEGPTPVSA LIHAATMVTA GVFLVCRMSP LYEFAPDAKN FIVIIGATTA 310 320 330 340 350 360 FFAATVGLVQ NDIKRVIAYS TCSQLGYMFV AAGVGVYSAA MFHLLTHAFF KAMLFLGAGS 370 380 390 400 410 420 VIHAMHHEQD MRNYGGLRKK IPLTFWAMMI GTFAITGVGI PLTHLGFAGF LSKDAIIESA 430 440 450 460 470 480 YAGSGYAFWL LVIAACFTSF YSWRLIFLTF YGKPRGDHHA HDHAHESPPV MTIPLGVLAI 490 500 510 520 530 540 GAVFAGMVWY GPFFGDHHKV TEYFHIAGAH HEAAEGEEAE HATAEAPVEH AVADTATAEG 550 560 570 580 590 600 EAAAEAEHAE IAAPVGGAIY MHPDNHIMDE AHHAPAWVKV SPFVAMVLGL ITAWTFYIAN 610 620 630 640 650 660 PSLPRRLAAH EPALYRFLLN KWYFDEIYEF IFVRPAKWLG RVLWKGGDGA VIDGTINGVA 670 680 690 700 MGLIPRLTRA AVRVQSGYLF HYAFAMVLGI VGLLIWVMMR GAH

References

[1]

"DNA sequencing of the seven remaining structural genes of the gene cluster encoding the energy-transducing NADH-quinone oxidoreductase of Paracoccus denitrificans." Xu X., Matsuno-Yagi A., Yagi T. Biochemistry 32:968-981(1993) [PubMed: 8422400] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 13543 / NRRL B-3784.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L02354 Genomic DNA. Translation: AAA25598.1.
PIR	H45456.
3D structure databases
ModBase	Search...
Protein family/group databases
TCDB	3.D.1.2.1. proton-translocating NADH dehydrogenase (NDH) family.
Enzyme and pathway databases
BRENDA	1.6.99.5. 59.
Family and domain databases
InterPro	IPR001750. NADH_UbQ/plastoQ_OxRdtase. IPR003916. NADH_UbQ_OxRdtase_chain5. IPR001516. NADH_UbQ_OxRdtase_chain5/L_N. IPR003945. NADHpl_OxRdtase_5. IPR018393. NADHpl_OxRdtase_5_sg. [Graphical view]
Pfam	PF00361. Oxidored_q1. 1 hit. PF00662. Oxidored_q1_N. 1 hit. [Graphical view]
PRINTS	PR01434. NADHDHGNASE5. PR01435. NPOXDRDTASE5.
TIGRFAMs	TIGR01974. NDH_I_L. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NQO12_PARDE

Accession

Primary (citable) accession number: P29924

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	October 13, 2009
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)