Reviewed, UniProtKB/Swiss-Prot P29923 (NQO11_PARDE)

Names and origin

Protein names

Recommended name:
    NADH-quinone oxidoreductase chain 11
    EC=1.6.99.5
Alternative name(s):
    NADH dehydrogenase I, chain 11
    NDH-1, chain 11

Gene names

Name:

nqo11

Organism

Paracoccus denitrificans

Taxonomic identifier

266 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Paracoccus

Protein attributes

Sequence length	101 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.
Catalytic activity	NADH + quinone = NAD+ + quinol.
Subunit structure	NDH-1 is composed of at least 14 different subunits, nqo1 to nqo14. The complex has a L-shaped structure, with the hydrophobic arm (subunits nqo7, nqo8, nqo10 to nqo14) embedded in the inner membrane and the hydrophilic peripheral arm (subunits nqo1 to nqo6, nqo9) protruding into the bacterial cytoplasm. The hydrophilic domain contains all the redox centers.
Subcellular location	Cell inner membrane; Multi-pass membrane protein. Ref.2
Sequence similarities	Belongs to the complex I subunit 4L family.

Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Ligand	NAD Ubiquinone
Molecular function	Oxidoreductase
PTM	Quinone
Gene Ontology (GO)
Biological process	ATP synthesis coupled electron transport Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NADH dehydrogenase (quinone) activity Inferred from electronic annotation. Source: EC quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 101	101	NADH-quinone oxidoreductase chain 11		PRO_0000118522
Regions
	Topological domain	1 – 3	3	Periplasmic Probable
	Transmembrane	4 – 24	21	Potential
	Topological domain	25 – 29	5	Cytoplasmic Probable
	Transmembrane	30 – 50	21	Potential
	Topological domain	51 – 64	14	Periplasmic Probable
	Transmembrane	65 – 85	21	Potential
	Topological domain	86 – 101	16	Cytoplasmic Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P29923-1 [UniParc]. Last modified April 1, 1993. Version 1. Checksum: A8061FD86A8B9AB8	FASTA	101	10,856
	10 20 30 40 50 60 MIGLTHYLVV GAILFVTGIF GIFVNRKNVI VILMSIELML LAVNINFVAF STHLGDLAGQ 70 80 90 100 VFTMFVLTVA AAEAAIGLAI LVVFFRNRGT IAVEDVNVMK G

References

[1]	"DNA sequencing of the seven remaining structural genes of the gene cluster encoding the energy-transducing NADH-quinone oxidoreductase of Paracoccus denitrificans." Xu X., Matsuno-Yagi A., Yagi T. Biochemistry 32:968-981(1993) [PubMed: 8422400] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 13543 / NRRL B-3784.
[2]	"Characterization of the membrane domain Nqo11 subunit of the proton-translocating NADH-quinone oxidoreductase of Paracoccus denitrificans." Kao M.-C., Di Bernardo S., Matsuno-Yagi A., Yagi T. Biochemistry 41:4377-4384(2002) [PubMed: 11914084] [Abstract] Cited for: SUBCELLULAR LOCATION, TOPOLOGY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L02354 Genomic DNA. Translation: AAA25597.1.
PIR	G45456.
3D structure databases
ModBase	Search...
Protein family/group databases
TCDB	3.D.1.2.1. proton-translocating NADH dehydrogenase (NDH) family.
Enzyme and pathway databases
BRENDA	1.6.99.5. 59.
Family and domain databases
InterPro	IPR001133. NADH_UbQ/Q_OxRdtase_chain4L. IPR017863. NADH_UbQ_OxRdtase_4L_subgr. [Graphical view]
PANTHER	PTHR11434. Oxidored_4L. 1 hit.
Pfam	PF00420. Oxidored_q2. 1 hit. [Graphical view]
ProDom	PD002107. NADH_dh_ubiq1. 1 hit. PD000359. Oxidred4L. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

NQO11_PARDE

Accession

Primary (citable) accession number: P29923

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	October 13, 2009
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)