P29922 (NQO10_PARDE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADH-quinone oxidoreductase chain 10
EC=1.6.99.5

Alternative name(s):
NADH dehydrogenase I, chain 10
NDH-1, chain 10

Gene names

Name:

nqo10

Organism

Paracoccus denitrificans

Taxonomic identifier

266 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Paracoccus

Protein attributes

Sequence length	200 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.
Catalytic activity	NADH + quinone = NAD+ + quinol.
Subunit structure	NDH-1 is composed of at least 14 different subunits, nqo1 to nqo14. The complex has a L-shaped structure, with the hydrophobic arm (subunits nqo7, nqo8, nqo10 to nqo14) embedded in the inner membrane and the hydrophilic peripheral arm (subunits nqo1 to nqo6, nqo9) protruding into the bacterial cytoplasm. The hydrophilic domain contains all the redox centers.
Subcellular location	Cell inner membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the complex I subunit 6 family.

Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	NAD Ubiquinone
Molecular function	Oxidoreductase
PTM	Quinone
Gene Ontology (GO)
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 200	200	NADH-quinone oxidoreductase chain 10		PRO_0000118368
Regions
	Transmembrane	2 – 22	21	Helical; Potential
	Transmembrane	26 – 46	21	Helical; Potential
	Transmembrane	51 – 71	21	Helical; Potential
	Transmembrane	90 – 110	21	Helical; Potential
	Transmembrane	144 – 164	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	P29922 [UniParc]. Last modified April 1, 1993. Version 1. Checksum: 9D3B421C33F4ACAE	FASTA	200	21,819
	10 20 30 40 50 60 MMTFAFYLFA ISACVAGFMV VIGRNPVHSV LWLILAFLSA AGLFVLQGAE FVAMLLVVVY 70 80 90 100 110 120 VGAVAVLFLF VVMMLDVDFA ELKGELARYL PLALVIGVVL LAQLGIAFSG WTPSDQAESL 130 140 150 160 170 180 RAAPVDAAVE NTLGLGLVLY DRYVLMFQLA GLVLLVAMIG AIVLTMRHRK DVKRQNVLEQ 190 200 MWRDPAKTME LKDVKPGQGL

References

[1]

"DNA sequencing of the seven remaining structural genes of the gene cluster encoding the energy-transducing NADH-quinone oxidoreductase of Paracoccus denitrificans." Xu X., Matsuno-Yagi A., Yagi T. Biochemistry 32:968-981(1993) [PubMed: 8422400] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 13543 / NRRL B-3784 / NRC 449.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L02354 Genomic DNA. Translation: AAA25596.1.
PIR	F45456.
3D structure databases
ModBase	Search...
Protein family/group databases
TCDB	3.D.1.2.1. H+ or Na+-translocating NADH dehydrogenase (NDH) family.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR001457. NADH_UbQ/plastoQ_OxRdtase_su6. [Graphical view]
Pfam	PF00499. Oxidored_q3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NQO10_PARDE

Accession

Primary (citable) accession number: P29922

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	June 28, 2011
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program