Reviewed, UniProtKB/Swiss-Prot P29920 (NQO8_PARDE)

Names and origin

Protein names

Recommended name:
    NADH-quinone oxidoreductase subunit 8
    EC=1.6.99.5
Alternative name(s):
    NADH dehydrogenase I subunit H
    NDH-1 subunit 8

Gene names

Name:

nqo8

Organism

Paracoccus denitrificans

Taxonomic identifier

266 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Paracoccus

Protein attributes

Sequence length	345 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient. HAMAP MF_01350
Catalytic activity	NADH + quinone = NAD+ + quinol. HAMAP MF_01350
Subunit structure	NDH-1 is composed of at least 14 different subunits, nqo1 to nqo14. The complex has a L-shaped structure, with the hydrophobic arm (subunits nqo7, nqo8, nqo10 to nqo14) embedded in the inner membrane and the hydrophilic peripheral arm (subunits nqo1 to nqo6, nqo9) protruding into the bacterial cytoplasm. The hydrophilic domain contains all the redox centers. HAMAP MF_01350
Subcellular location	Cell inner membrane; Multi-pass membrane protein. HAMAP MF_01350
Sequence similarities	Belongs to the complex I subunit 1 family.

Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Ligand	NAD Ubiquinone
Molecular function	Oxidoreductase
PTM	Quinone
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: HAMAP
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NADH dehydrogenase (quinone) activity Inferred from electronic annotation. Source: EC quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 345	345	NADH-quinone oxidoreductase subunit 8 HAMAP MF_01350		PRO_0000117521
Regions
	Transmembrane	15 – 35	21	Potential
	Transmembrane	82 – 102	21	Potential
	Transmembrane	115 – 135	21	Potential
	Transmembrane	161 – 181	21	Potential
	Transmembrane	190 – 210	21	Potential
	Transmembrane	240 – 262	23	Potential
	Transmembrane	278 – 298	21	Potential
	Transmembrane	309 – 329	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	P29920-1 [UniParc]. Last modified April 1, 1993. Version 1. Checksum: E33B667E569506B4	FASTA	345	38,751
	10 20 30 40 50 60 MAEFWASPYG FALSMLLQGL AVIAFVMGSL IFMVYGDRKI WAAVQMRRGP NVVGPWGLLQ 70 80 90 100 110 120 TFADALKYIV KEIVIPAGAD KFVYFLAPFL SMMLALFAFV VIPFDEGWVM ANINVGILFI 130 140 150 160 170 180 FAASSLEVYG VIMGGWASNS KYPFLASLRS AAQMISYEVS LGLIIIGIII STGSMNLTAI 190 200 210 220 230 240 VEAHGGDYGL LNWYWLPHLP MVVLFFVSAL AECNRPPFDL VEAESELVAG FMTEYSSTPY 250 260 270 280 290 300 LLFMAGEYIA MYLMCALLSL LFFGGWLSPV PFIADGWWWM VIKMWFWFYM FAMVKAIVPR 310 320 330 340 YRYDQLMRIG WKVFLPLSLG WVVLVAILAR YEILGGFWAR FAVGG

References

[1]

"DNA sequencing of the seven remaining structural genes of the gene cluster encoding the energy-transducing NADH-quinone oxidoreductase of Paracoccus denitrificans." Xu X., Matsuno-Yagi A., Yagi T. Biochemistry 32:968-981(1993) [PubMed: 8422400] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 13543 / NRRL B-3784.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L02354 Genomic DNA. Translation: AAA25592.1.
PIR	C45456.
3D structure databases
ModBase	Search...
Protein family/group databases
TCDB	3.D.1.2.1. proton-translocating NADH dehydrogenase (NDH) family.
Enzyme and pathway databases
BRENDA	1.6.99.5. 59.
Family and domain databases
HAMAP	MF_01350. [Tree]
InterPro	IPR001694. NADH_UbQ_OxRdtase_su1. IPR018086. NADH_UbQ_OxRdtase_su1_CS. [Graphical view]
PANTHER	PTHR11432. Resp_NADH_DH_1. 1 hit.
Pfam	PF00146. NADHdh. 1 hit. [Graphical view]
PROSITE	PS00667. COMPLEX1_ND1_1. 1 hit. PS00668. COMPLEX1_ND1_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NQO8_PARDE

Accession

Primary (citable) accession number: P29920

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	June 16, 2009
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)