Reviewed, UniProtKB/Swiss-Prot P29917 (NQO5_PARDE)

Names and origin

Protein names

Recommended name:
    NADH-quinone oxidoreductase chain 5
    EC=1.6.99.5
Alternative name(s):
    NADH dehydrogenase I, chain 5
    NDH-1, chain 5

Gene names

Name:

nqo5

Organism

Paracoccus denitrificans

Taxonomic identifier

266 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Paracoccus

Protein attributes

Sequence length	207 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient. HAMAP MF_01357
Catalytic activity	NADH + quinone = NAD+ + quinol. HAMAP MF_01357
Subunit structure	NDH-1 is composed of at least 14 different subunits, nqo1 to nqo14. The complex has a L-shaped structure, with the hydrophobic arm (subunits nqo7, nqo8, nqo10 to nqo14) embedded in the inner membrane and the hydrophilic peripheral arm (subunits nqo1 to nqo6, nqo9) protruding into the bacterial cytoplasm. The hydrophilic domain contains all the redox centers. HAMAP MF_01357
Subcellular location	Cell inner membrane; Peripheral membrane protein. HAMAP MF_01357
Sequence similarities	Belongs to the complex I 30 kDa subunit family.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Ligand	NAD Ubiquinone
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: HAMAP photosynthesis, light reaction Inferred from electronic annotation. Source: HAMAP transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.1
	Chain	2 – 207	206	NADH-quinone oxidoreductase chain 5 HAMAP MF_01357		PRO_0000118668

Sequences

	Sequence		Length	Mass (Da)
	P29917-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 070DFDEBE70FEC6D	FASTA	207	23,731
	10 20 30 40 50 60 MSEALSDEAL LELAEHIAVR RENDVISTQA VGELTVNATL SGVIGLIEFL RNDPNCRFST 70 80 90 100 110 120 LIDITAVDNP ARPARFDVVY HLLSMYQNQR IRVKVQVRED ELVPSLIGVF PGANWYEREV 130 140 150 160 170 180 FDLFGILFSG HSDLRRILTD YGFRGHPLRK DFPTTGYVEV RWSDIEKRVV YEPVNLVQEY 190 200 RQFDFLSPWE GAKYVLPGDE KAPEAKK

References

[1]

"Gene cluster of the energy-transducing NADH-quinone oxidoreductase of Paracoccus denitrificans: characterization of four structural gene products." Xu X., Matsuno-Yagi A., Yagi T. Biochemistry 31:6925-6932(1992) [PubMed: 1637825] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-15. Strain: ATCC 13543 / NRRL B-3784.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M93015 Unassigned DNA. Translation: AAA03037.1. Sequence problems.
PIR	D42573.
3D structure databases
ModBase	Search...
Protein family/group databases
TCDB	3.D.1.2.1. proton-translocating NADH dehydrogenase (NDH) family.
Enzyme and pathway databases
BRENDA	1.6.99.5. 59.
Family and domain databases
HAMAP	MF_01357. [Tree]
InterPro	IPR010218. NADH_DH_csu. IPR001268. NADH_UbQ_OxRdtase_30kDa_su. [Graphical view]
Pfam	PF00329. Complex1_30kDa. 1 hit. [Graphical view]
ProDom	PD001581. Complex1_30K. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01961. NuoC_fam. 1 hit.
PROSITE	PS00542. COMPLEX1_30K. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NQO5_PARDE

Accession

Primary (citable) accession number: P29917

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 57 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)