P29913 (NQO1_PARDE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADH-quinone oxidoreductase chain 1
EC=1.6.99.5

Alternative name(s):
NADH dehydrogenase I, chain 1
NDH-1, chain 1

Gene names

Name:

nqo1

Organism

Paracoccus denitrificans

Taxonomic identifier

266 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Paracoccus

Protein attributes

Sequence length	431 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.
Catalytic activity	NADH + quinone = NAD+ + quinol.
Cofactor	Binds 1 FMN Potential. Binds 1 4Fe-4S cluster Potential.
Subunit structure	NDH-1 is composed of at least 14 different subunits, nqo1 to nqo14. The complex has a L-shaped structure, with the hydrophobic arm (subunits nqo7, nqo8, nqo10 to nqo14) embedded in the inner membrane and the hydrophilic peripheral arm (subunits nqo1 to nqo6, nqo9) protruding into the bacterial cytoplasm. The hydrophilic domain contains all the redox centers.
Subcellular location	Cell inner membrane; Peripheral membrane protein.
Sequence similarities	Belongs to the complex I 51 kDa subunit family.

Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Membrane
Ligand	4Fe-4S FMN Flavoprotein Iron Iron-sulfur Metal-binding NAD Ubiquinone
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Direct protein sequencing
Gene Ontology (GO)
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW FMN binding Inferred from electronic annotation. Source: InterPro NAD binding Inferred from electronic annotation. Source: InterPro NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 431	431	NADH-quinone oxidoreductase chain 1		PRO_0000118566
Regions
	Nucleotide binding	54 – 63	10	NAD By similarity
	Nucleotide binding	167 – 214	48	FMN By similarity
Sites
	Metal binding	346	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	349	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	352	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	392	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	P29913 [UniParc]. Last modified April 1, 1993. Version 1. Checksum: AA25C5A0A7570048	FASTA	431	47,191
	10 20 30 40 50 60 MLNDQDRIFT NLYGMGDRSL AGAKKRGHWD GTAAIIQRGR DKIIDEMKAS GLRGRGGAGF 70 80 90 100 110 120 PTGMKWSFMP KESDGRPSYL VINADESEPA TCKDREIMRH DPHTLIEGAL IASFAMGAHA 130 140 150 160 170 180 AYIYIRGEFI REREALQAAI DECYDAGLLG RNAAGSGWDF DLYLHHGAGA YICGEETALL 190 200 210 220 230 240 ESLEGKKGMP RMKPPFPAGA GLYGCPTTVN NVESIAVVPT ILRRGAEWFA SFGRPNNAGV 250 260 270 280 290 300 KLFGLTGHVN TPCVVEEAMS IPMRELIEKH GGGIRGGWKN LKAVIPGGAS CPVLTAEQCE 310 320 330 340 350 360 NAIMDYDGMR DVRSSFGTAC MIVMDQSTDV VKAIWRLSKF FKHESCGQCT PCREGTGWMM 370 380 390 400 410 420 RVMERLVRGD AEVEEIDMLF DVTKQVEGHT ICALGDAAAW PIQGLIRNFR EEIEDRIKAK 430 RTGRMGAMAA E

References

[1]	"The NADH-binding subunit of the energy-transducing NADH-ubiquinone oxidoreductase of Paracoccus denitrificans: gene cloning and deduced primary structure." Xu X., Matsuno-Yagi A., Yagi T. Biochemistry 30:6422-6428(1991) [PubMed: 1905152] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-37. Strain: ATCC 13543 / NRRL B-3784 / NRC 449.
[2]	"Characterization of the 25-kilodalton subunit of the energy-transducing NADH-ubiquinone oxidoreductase of Paracoccus denitrificans: sequence similarity to the 24-kilodalton subunit of the flavoprotein fraction of mammalian complex I." Xu X., Matsuno-Yagi A., Yagi T. Biochemistry 30:8678-8684(1991) [PubMed: 1909571] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-8, PROTEIN SEQUENCE OF 1-37 AND 194-203. Strain: ATCC 13543 / NRRL B-3784 / NRC 449.
[3]	"Structural features of the 66-kDa subunit of the energy-transducing NADH-ubiquinone oxidoreductase (NDH-1) of Paracoccus denitrificans." Xu X., Matsuno-Yagi A., Yagi T. Arch. Biochem. Biophys. 296:40-48(1992) [PubMed: 1605643] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 416-431.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M64432 Genomic DNA. Translation: AAA25585.1.
PIR	A39588.
3D structure databases
ProteinModelPortal	P29913.
ModBase	Search...
Protein family/group databases
TCDB	3.D.1.2.1. H+ or Na+-translocating NADH dehydrogenase (NDH) family.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR001949. NADH-UbQ_OxRdtase_51kDa_CS. IPR019575. NADH-UbQ_OxRdtase_Fsu_4Fe4S-bd. IPR011537. NADH-UbQ_OxRdtase_suF. IPR011538. NADH_UbQ_OxRdtase_51kDa_su. IPR019554. Soluble_ligand-bd. [Graphical view]
Pfam	PF01512. Complex1_51K. 1 hit. PF10589. NADH_4Fe-4S. 1 hit. PF10531. SLBB. 1 hit. [Graphical view]
SMART	SM00928. NADH_4Fe-4S. 1 hit. [Graphical view]
TIGRFAMs	TIGR01959. NuoF_fam. 1 hit.
PROSITE	PS00644. COMPLEX1_51K_1. 1 hit. PS00645. COMPLEX1_51K_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NQO1_PARDE

Accession

Primary (citable) accession number: P29913

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	September 21, 2011
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program