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P29883

- RIR2_VACCP

UniProt

P29883 - RIR2_VACCP

Protein

Ribonucleoside-diphosphate reductase small chain

Gene

F14

Organism
Vaccinia virus (strain L-IVP) (VACV)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 76 (01 Oct 2014)
      Sequence version 1 (01 Apr 1993)
      Previous versions | rss
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    Functioni

    Ribonucleoside-diphosphate reductase holoenzyme provides the precursors necessary for viral DNA synthesis. Allows virus growth in non-dividing cells. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides By similarity.By similarity

    Catalytic activityi

    2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.PROSITE-ProRule annotation

    Cofactori

    Binds 2 iron ions per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi70 – 701Iron 1PROSITE-ProRule annotation
    Metal bindingi101 – 1011Iron 1PROSITE-ProRule annotation
    Metal bindingi101 – 1011Iron 2By similarity
    Metal bindingi104 – 1041Iron 1PROSITE-ProRule annotation
    Active sitei108 – 1081PROSITE-ProRule annotation
    Metal bindingi163 – 1631Iron 2By similarity
    Metal bindingi197 – 1971Iron 2By similarity
    Metal bindingi200 – 2001Iron 2By similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-EC

    GO - Biological processi

    1. deoxyribonucleoside diphosphate metabolic process Source: InterPro
    2. DNA replication Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    DNA replication

    Keywords - Ligandi

    Iron, Metal-binding

    Enzyme and pathway databases

    UniPathwayiUPA00326.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribonucleoside-diphosphate reductase small chain (EC:1.17.4.1)
    Alternative name(s):
    Ribonucleotide reductase small subunit
    Gene namesi
    ORF Names:F14
    OrganismiVaccinia virus (strain L-IVP) (VACV)
    Taxonomic identifieri31531 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePoxviridaeChordopoxvirinaeOrthopoxvirusVaccinia virus
    Virus hostiHomo sapiens (Human) [TaxID: 9606]

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 319319Ribonucleoside-diphosphate reductase small chainPRO_0000190497Add
    BLAST

    Expressioni

    Inductioni

    Expressed early in the viral replicative cycle.

    Keywords - Developmental stagei

    Early protein

    Interactioni

    Subunit structurei

    Heterotetramer composed of a homodimer of the large subunit (R1) and a homodimer of the small subunit (R2). Larger multisubunit protein complex are also active, composed of (R1)n(R2)n By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP29883.
    SMRiP29883. Positions 2-282.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Family and domain databases

    Gene3Di1.10.620.20. 1 hit.
    InterProiIPR009078. Ferritin-like_SF.
    IPR012348. RNR-rel.
    IPR000358. RNR_small.
    [Graphical view]
    PANTHERiPTHR23409. PTHR23409. 1 hit.
    PfamiPF00268. Ribonuc_red_sm. 1 hit.
    [Graphical view]
    SUPFAMiSSF47240. SSF47240. 1 hit.
    PROSITEiPS00368. RIBORED_SMALL. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P29883-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEPILAPNPN RFVIFPIQYH DIWNMYKKAE ASFWTVEEVD ISKDINDWNK    50
    VTPDEKYFIK HVLAFFAASD GIVNENLAER FCTEVQITEA RCFYGFRMAI 100
    ENIHSEMYSL LIDTYVKDSN EKNYLFNAIE TMPCVKKKAD WAQKWIHDSA 150
    GYGERLIAFA AVEGIFFSGS FASIFWLKKR GLMPGLTFSN ELISRDEGLH 200
    CDFACLMFKH LLHPPSEETV RSIITDAVSI EQEFLTAALP VKLIGMNCEM 250
    MKTYIEFVAD RLISELGFKK IYNVTNPFDF MENISLEGKT NFFEKRVGEY 300
    QKMGVMSQKD NHFSLDVDF 319
    Length:319
    Mass (Da):36,960
    Last modified:April 1, 1993 - v1
    Checksum:i9CF2C3322B313BB0
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M57977 Genomic DNA. Translation: AAA48294.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M57977 Genomic DNA. Translation: AAA48294.1 .

    3D structure databases

    ProteinModelPortali P29883.
    SMRi P29883. Positions 2-282.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00326 .

    Family and domain databases

    Gene3Di 1.10.620.20. 1 hit.
    InterProi IPR009078. Ferritin-like_SF.
    IPR012348. RNR-rel.
    IPR000358. RNR_small.
    [Graphical view ]
    PANTHERi PTHR23409. PTHR23409. 1 hit.
    Pfami PF00268. Ribonuc_red_sm. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47240. SSF47240. 1 hit.
    PROSITEi PS00368. RIBORED_SMALL. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structural-functional organization of segment of vaccinia virus genome."
      Mikryukov N.N., Chizhikov V.E., Prikhod'Ko G.G., Urmmanov I.M., Serpinskii O.I., Blinov V.M., Nikulin A.E., Vasilenko S.K.
      Biotekhnologiya 4:442-449(1988)
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

    Entry informationi

    Entry nameiRIR2_VACCP
    AccessioniPrimary (citable) accession number: P29883
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1993
    Last sequence update: April 1, 1993
    Last modified: October 1, 2014
    This is version 76 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3