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P29848 (CYSM_SALTY) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 91. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Cysteine synthase B
Short name=CSase B
EC=2.5.1.47
Alternative name(s):
O-acetylserine (thiol)-lyase B
Short name=OAS-TL B
O-acetylserine sulfhydrylase B
Gene names
Name:cysM
Ordered Locus Names:STM2440
OrganismSalmonella typhimurium
Taxonomic identifier90371 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella

Protein attributes

Sequence length303 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Two cysteine synthase enzymes are found. Both catalyze the same reaction. Cysteine synthase B can also use thiosulfate in place of sulfide to give cysteine thiosulfonate as a product.

Catalytic activity

O(3)-acetyl-L-serine + H2S = L-cysteine + acetate.

Cofactor

Pyridoxal phosphate.

Pathway

Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-serine: step 2/2.

Sequence similarities

Belongs to the cysteine synthase/cystathionine beta-synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 303303Cysteine synthase B
PRO_0000167109

Regions

Region174 – 1785Pyridoxal phosphate binding By similarity

Sites

Binding site711Pyridoxal phosphate By similarity
Binding site2551Pyridoxal phosphate By similarity

Amino acid modifications

Modified residue411N6-(pyridoxal phosphate)lysine By similarity

Secondary structure

1 ........................................................... 303
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P29848 [UniParc].

Last modified April 1, 1993. Version 1.
Checksum: 95435A8C09209EFD

FASTA30332,645
        10         20         30         40         50         60 
MNTLEQTIGN TPLVKLQRLG PDNGSEIWVK LEGNNPAGSV KDRAALSMIV EAEKRGEIKP 

        70         80         90        100        110        120 
GDVLIEATSG NTGIALAMIA ALKGYRMKLL MPDNMSQERR AAMRAYGAEL ILVTKEQGME 

       130        140        150        160        170        180 
GARDLALAMS ERGEGKLLDQ FNNPDNPYAH YTTTGPEIWR QTSGRITHFV SSMGTTGTIT 

       190        200        210        220        230        240 
GVSRFLREQE KPVTIVGLQP EEGSSIPGIR RWPAEYMPGI FNASLVDEVL DIHQNDAENT 

       250        260        270        280        290        300 
MRELAVREGI FCGVSSGGAV AGALRVARAT PGAIVVAIIC DRGDRYLSTG VFGEEHFSQG 


AGI

« Hide

References

« Hide 'large scale' references
[1]Sivaprasad A.V., Kuczek E.S., Bawden C.S., Rogers G.E.
Submitted (MAY-1991) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: LT2.
[2]"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2."
McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. expand/collapse author list , Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K.
Nature 413:852-856(2001) [PubMed: 11677609] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: LT2 / SGSC1412 / ATCC 700720.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X59595 Genomic DNA. Translation: CAA42164.1.
AE006468 Genomic DNA. Translation: AAL21334.1.
PIRS29567.
RefSeqNP_461375.1. NC_003197.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2JC3X-ray2.30A/B/C/D/E/F/G/H1-303[»]
ProteinModelPortalP29848.
SMRP29848. Positions 1-293.
ModBaseSearch...

Proteomic databases

PRIDEP29848.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1253962.
GenomeReviewsGene locus STM2440 in contig AE006468_GR.
KEGGstm:STM2440.
PATRIC32383511. VBISalEnt20916_2578.

Phylogenomic databases

HOGENOMHBG748215.
OMATIEACIG.
ProtClustDBPRK11761.

Enzyme and pathway databases

BioCycSTYP99287:STM2440-MONOMER.
BRENDA2.5.1.47. 5542.

Family and domain databases

InterProIPR001216. Cys_synth_BS.
IPR005856. Cys_synthKM.
IPR005858. CysM.
IPR001926. PyrdxlP-dep_enz_bsu.
[Graphical view]
KOK12339.
PfamPF00291. PALP. 1 hit.
[Graphical view]
SUPFAMSSF53686. PyrdxlP-dep_enz_bsu. 1 hit.
TIGRFAMsTIGR01136. CysKM. 1 hit.
TIGR01138. CysM. 1 hit.
PROSITEPS00901. CYS_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCYSM_SALTY
AccessionPrimary (citable) accession number: P29848
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: January 25, 2012
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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