P29844 (HSP7C_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Heat shock 70 kDa protein cognate 3

Alternative name(s):
78 kDa glucose-regulated protein homolog
GRP 78
Heat shock protein cognate 72

Gene names

Name:	Hsc70-3
Synonyms:	Hsc3
ORF Names:	CG4147

Organism

Drosophila melanogaster (Fruit fly) [Reference proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Acalyptratae › Ephydroidea › Drosophilidae › Drosophilinae › Drosophilini › Drosophilina › Drosophiliti › Drosophila › Sophophora › melanogaster group › melanogaster subgroup

Protein attributes

Sequence length	656 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Probably plays a role in facilitating the assembly of multimeric protein complexes inside the ER.
Subcellular location	Endoplasmic reticulum lumen.
Sequence similarities	Belongs to the heat shock protein 70 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum
Domain	Signal
Ligand	ATP-binding Nucleotide-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	RNA interference Inferred from mutant phenotype PubMed 16882716. Source: FlyBase centrosome duplication Inferred from mutant phenotype PubMed 20818332. Source: FlyBase sleep Traceable author statement PubMed 14505361. Source: FlyBase
Cellular_component	endoplasmic reticulum Inferred from direct assay PubMed 17170705. Source: FlyBase endoplasmic reticulum lumen Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular space Inferred from direct assay PubMed 16670001. Source: FlyBase lipid particle Inferred from direct assay PubMed 16543254PubMed 16979555. Source: FlyBase microtubule associated complex Inferred from direct assay PubMed 18433294. Source: FlyBase
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	Potential
	Chain	19 – 656	638	Heat shock 70 kDa protein cognate 3		PRO_0000013546
Regions
	Motif	653 – 656	4	Prevents secretion from ER Potential
Experimental info
	Sequence conflict	313	1	Missing in AAO45194. Ref.4
	Sequence conflict	507	1	G → A in AAA28626. Ref.1
	Sequence conflict	510	1	Q → T in AAA28626. Ref.1
	Sequence conflict	592	1	N → T in AAA28626. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P29844 [UniParc]. Last modified July 5, 2004. Version 2. Checksum: 06A68F839A06337D	FASTA	656	72,261
	10 20 30 40 50 60 MKLCILLAVV AFVGLSLGEE KKEKDKELGT VIGIDLGTTY SCVGVYKNGR VEIIANDQGN 70 80 90 100 110 120 RITPSYVAFT ADGERLIGDA AKNQLTTNPE NTVFDAKRLI GREWSDTNVQ HDIKFFPFKV 130 140 150 160 170 180 VEKNSKPHIS VDTSQGAKVF APEEISAMVL GKMKETAEAY LGKKVTHAVV TVPAYFNDAQ 190 200 210 220 230 240 RQATKDAGVI AGLQVMRIIN EPTAAAIAYG LDKKEGEKNV LVFDLGGGTF DVSLLTIDNG 250 260 270 280 290 300 VFEVVATNGD THLGGEDFDQ RVMDHFIKLY KKKKGKDIRK DNRAVQKLRR EVEKAKRALS 310 320 330 340 350 360 GSHQVRIEIE SFFEGDDFSE TLTRAKFEEL NLDLFRSTLK PVQKVLEDAD MNKKDVHEIV 370 380 390 400 410 420 LVGGSTRIPK VQQLVKDFFG GKEPSRGINP DEAVAYGAAV QAGVLSGEQD TDAIVLLDVN 430 440 450 460 470 480 PLTMGIETVG GVMTKLIPRN TVIPTKKSQV FSTASDNQHT VTIQVYEGER PMTKDNHLLG 490 500 510 520 530 540 KFDLTGIPPA PRGIPQIEVS FEIDANGILQ VSAEDKGTGN KEKIVITNDQ NRLTPEDIDR 550 560 570 580 590 600 MIRDAEKFAD EDKKLKERVE SRNELESYAY SLKNQIGDKD KLGAKLSDDE KNKLESAIDE 610 620 630 640 650 SIKWLEQNPD ADPEEYKKQK KDLEAIVQPV IAKLYQGAGG APPPEGGDDA DLKDEL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genomic structure and sequence analysis of Drosophila melanogaster HSC70 genes." Rubin D.M., Mehta A., Zhu J., Shoham S., Chen X.J., Wells Q., Palter K.B. Gene 128:155-163(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Larva.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[4]	Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J.W., Champe M., Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.A., Gonzalez M., Guarin H., Kronmiller B., Li P.W., Liao G., Miranda A. , Mungall C.J., Nunoo J., Pacleb J.M., Paragas V., Park S., Patel S., Phouanenavong S., Wan K.H., Yu C., Lewis S.E., Rubin G.M., Celniker S.E. Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Head.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L01498 mRNA. Translation: AAA28626.1. AE014298 Genomic DNA. Translation: AAF48095.1. AE014298 Genomic DNA. Translation: AAN09299.1. AE014298 Genomic DNA. Translation: AAN09300.1. AE014298 Genomic DNA. Translation: AAN09301.1. BT004838 mRNA. Translation: AAO45194.1.
PIR	JN0666.
RefSeq	NP_511132.2. NM_078577.2. NP_727563.1. NM_167306.1. NP_727564.1. NM_167307.1. NP_727565.1. NM_167308.1.
UniGene	Dm.2924.
3D structure databases
ProteinModelPortal	P29844.
SMR	P29844. Positions 27-598.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-19696N.
IntAct	P29844. 1 interaction.
MINT	MINT-1330773.
Proteomic databases
PaxDb	P29844.
PRIDE	P29844.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0073608; FBpp0073445; FBgn0001218. FBtr0073609; FBpp0073446; FBgn0001218. FBtr0073610; FBpp0073447; FBgn0001218. FBtr0073611; FBpp0073448; FBgn0001218.
GeneID	32133.
KEGG	dme:Dmel_CG4147.
Organism-specific databases
CTD	32133.
FlyBase	FBgn0001218. Hsc70-3.
Phylogenomic databases
eggNOG	COG0443.
GeneTree	ENSGT00700000104620.
InParanoid	P29844.
KO	K09490.
OMA	VIAKLYQ.
OrthoDB	EOG4FXPPK.
PhylomeDB	P29844.
Gene expression databases
Bgee	P29844.
GermOnline	CG4147. Drosophila melanogaster.
Family and domain databases
InterPro	IPR018181. Heat_shock_70_CS. IPR013126. Hsp_70_fam. [Graphical view]
Pfam	PF00012. HSP70. 1 hit. [Graphical view]
PRINTS	PR00301. HEATSHOCK70.
PROSITE	PS00014. ER_TARGET. 1 hit. PS00297. HSP70_1. 1 hit. PS00329. HSP70_2. 1 hit. PS01036. HSP70_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	Hsc70-3. drosophila.
GenomeRNAi	32133.
NextBio	777024.

Entry information

Entry name

HSP7C_DROME

Accession

Primary (citable) accession number: P29844
Secondary accession number(s): A4V4C4 , Q86NM3, Q9VYU2, Q9VYU3

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	July 5, 2004
Last modified:	May 1, 2013
This is version 103 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project