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Reviewed, UniProtKB/Swiss-Prot P29837 (POLG_LANVT)

Last modified June 16, 2009. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Genome polyprotein
Cleaved into the following 10 chains:
    1- Recommended name:
            Protein C
        Alternative name(s):
            Core protein
            Capsid protein
    2- Recommended name:
            Small envelope protein M
        Alternative name(s):
            Matrix protein
    3- Recommended name:
            Envelope protein E
    4- Recommended name:
            Non-structural protein 1
                Short name=NS1
    5- Recommended name:
            Non-structural protein 2A
                Short name=NS2A
    6- Recommended name:
            Flavivirin protease NS2B regulatory subunit
    7- Recommended name:
            Flavivirin protease NS3 catalytic subunit
              EC=3.4.21.91
    8- Recommended name:
            Non-structural protein 4A
                Short name=NS4A
    9- Recommended name:
            Non-structural protein 4B
                Short name=NS4B
    10- Recommended name:
            RNA-directed RNA polymerase
              EC=2.7.7.48
        Alternative name(s):
            NS5
OrganismLangat virus (strain TP21) [Complete proteome]
Taxonomic identifier31638 [NCBI]
Taxonomic lineageViruses › ssRNA positive-strand viruses, no DNA stage › Flaviviridae › Flavivirus › tick-borne encephalitis virus group
Virus hostIxodida (ticks) [TaxID: 6935]
Homo sapiens (Human) [TaxID: 9606]

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Protein attributes

Sequence length3414 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

The small proteins NS2A, NS4A and NS4B are hydrophobic, suggesting a possible membrane-related function. NS5 may play a role in the viral RNA replication. The NS2B/NS3 protease complex processes the viral polyprotein.

Catalytic activity

Selective hydrolysis of -Xaa-Xaa-|-Yaa- bonds in which each of the Xaa can be either Arg or Lys and Yaa can be either Ser or Ala.

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

Subunit structure

NS3 and NS2B form a heterodimer. NS3 is the catalytic subunit, whereas NS2B strongly stimulates the latter By similarity.

Subcellular location

Protein C: Virion Potential. Host membrane; Single-pass membrane protein Potential.

Small envelope protein M: Virion Potential. Host membrane; Single-pass membrane protein Potential.

Envelope protein E: Virion Potential. Host membrane; Multi-pass membrane protein Potential.

Non-structural protein 4A: Host membrane; Single-pass membrane protein Potential.

Non-structural protein 4B: Host membrane; Multi-pass membrane protein Potential.

Post-translational modification

Specific enzymatic cleavages in vivo yield mature proteins By similarity.

Miscellaneous

The virion of this virus is a nucleocapsid covered by a lipoprotein envelope. The envelope contains two proteins: the protein M and glycoprotein E. The nucleocapsid is a complex of protein C and mRNA. In immature particles, there are 60 icosaedrally organized trimeric spikes on the surface. Each spike consists of three heterodimers of envelope protein M precursor (prM) and envelope protein E By similarity.

Sequence similarities

Contains 1 helicase ATP-binding domain.

Contains 1 helicase C-terminal domain.

Contains 1 peptidase S7 domain.

Contains 1 RdRp catalytic domain.

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Ontologies

Keywords
   Biological processRNA replication
   Cellular componentCapsid protein
Core protein
Envelope protein
Membrane
Virion
   DomainTransmembrane
   LigandATP-binding
Nucleotide-binding
   Molecular functionHelicase
Hydrolase
Nucleotidyltransferase
RNA-directed RNA polymerase
Transferase
   PTMCleavage on pair of basic residues
Disulfide bond
Glycoprotein
   Technical term3D-structure
Complete proteome
Gene Ontology (GO)
   Biological processRNA metabolic process

Inferred from electronic annotation. Source: InterPro

methylation

Inferred from electronic annotation. Source: InterPro

transcription, RNA-dependent

Inferred from electronic annotation. Source: UniProtKB-KW

viral genome replication

Inferred from electronic annotation. Source: InterPro

   Cellular componentintegral to membrane

Inferred from electronic annotation. Source: UniProtKB-KW

viral envelope

Inferred from electronic annotation. Source: UniProtKB-KW

viral nucleocapsid

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

ATP-dependent helicase activity

Inferred from electronic annotation. Source: InterPro

RNA helicase activity

Inferred from electronic annotation. Source: InterPro

RNA-directed RNA polymerase activity

Inferred from electronic annotation. Source: UniProtKB-KW

double-stranded RNA binding

Inferred from electronic annotation. Source: InterPro

methyltransferase activity

Inferred from electronic annotation. Source: InterPro

serine-type endopeptidase activity

Inferred from electronic annotation. Source: InterPro

structural molecule activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed; by host
Chain2 – 112111Protein C Potential
PRO_0000037688
Propeptide113 – 20593
PRO_0000037689
Chain206 – 28075Small envelope protein M Potential
PRO_0000037690
Chain281 – 776496Envelope protein E Potential
PRO_0000037691
Chain777 – 1128352Non-structural protein 1 Potential
PRO_0000037692
Chain1129 – 1358230Non-structural protein 2A Potential
PRO_0000037693
Chain1359 – 1489131Flavivirin protease NS2B regulatory subunit Potential
PRO_0000037694
Chain1490 – 2110621Flavivirin protease NS3 catalytic subunit Potential
PRO_0000037695
Chain2111 – 2259149Non-structural protein 4A Potential
PRO_0000037696
Chain2260 – 2511252Non-structural protein 4B Potential
PRO_0000037697
Chain2512 – 3414903RNA-directed RNA polymerase Potential
PRO_0000037698

Regions

Transmembrane103 – 11917 Potential
Transmembrane262 – 27817 Potential
Transmembrane728 – 74417 Potential
Transmembrane758 – 77417 Potential
Domain1496 – 1672177Peptidase S7
Domain1675 – 1831157Helicase ATP-binding
Domain1841 – 2000160Helicase C-terminal
Domain3040 – 3189150RdRp catalytic
Nucleotide binding1688 – 16958ATP Potential
Motif1779 – 17824DEAH box

Sites

Active site15431Charge relay system By similarity
Active site15671Charge relay system By similarity
Active site16271Charge relay system By similarity

Amino acid modifications

Glycosylation1441N-linked (GlcNAc...); by host Potential
Glycosylation4341N-linked (GlcNAc...); by host Potential
Disulfide bond283 ↔ 310 By similarity
Disulfide bond340 ↔ 396 By similarity
Disulfide bond354 ↔ 385 By similarity
Disulfide bond372 ↔ 401 By similarity
Disulfide bond466 ↔ 570 By similarity
Disulfide bond587 ↔ 618 By similarity

Secondary structure

1 ............... 3414
Helix Strand Turn

Details...

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Sequences

Sequence LengthMass (Da)Tools
P29837-1 [UniParc].

Last modified February 1, 1994. Version 2.
Checksum: 59CB7E95DD70D82E

FASTA3,414378,023
        10         20         30         40         50         60 
MAGKAVLKGK GGGPPRRASK VAPKKTRQLR VQMPNGLVLM RMLGVLWHAL TGTARSPVLK 

        70         80         90        100        110        120 
AFWKVVPLKQ ATLALRKIKR TVSTLMVGLH RRGSRRTTID WMTPLLITVM LGMCLTATVR 

       130        140        150        160        170        180 
RERDGSMVIR AEGRDAATQV RVENGTCVIL ATDMGSWCDD SLAYECVTID QGEEPVDVDC 

       190        200        210        220        230        240 
FCRGVEKVTL EYGRCGRREG SRSRRSVLIP SHAQRDLTGR GHQWLEGEAV KAHLTRVEGW 

       250        260        270        280        290        300 
VWKNKLFTLS LVMVAWLMVD GLLPRILIVV VALALVPAYA SRCTHLENRD FVTGVQGTTR 

       310        320        330        340        350        360 
LTLVLELGGC VTVTADGKPS LDVWLDSIYQ ESPAQTREYC LHAKLTGTKV AARCPTMGPA 

       370        380        390        400        410        420 
TLPEEHQSGT VCKRDQSDRG WGNHCGLFGK GSIVTCVKFT CEDKKKATGH VYDVNKITYT 

       430        440        450        460        470        480 
IKVEPHTGEF VAANETHSGR KSASFTVSSE KTILTLGDYG DVSLLCRVAS GVDLAQTVVL 

       490        500        510        520        530        540 
ALDKTHEHLP TAWQVHRDWF NDLALPWKHD GAEAWNEAGR LVEFGTPHAV KMDVFNLGDQ 

       550        560        570        580        590        600 
TGVLLKSLAG VPVASIEGTK YHLKSGHVTC EVGLEKLKMK GLTYTVCDKT KFTWKRAPTD 

       610        620        630        640        650        660 
SGHDTVVMEV GFSGTRPCRI PVRAVAHGVP EVNVAMLITP NPTMENNGGG FIEMQLPPGD 

       670        680        690        700        710        720 
NIIYVGDLNH QWFQKGSSIG RVLQKTRKGI ERLTVLGEHA WDFGSVGGVM TSIGRAMHTV 

       730        740        750        760        770        780 
LGGAFNTLLG GVGFLPKILL GVAMAWLGLN MRNPTLSMGF LLSGGLVLAM TLGVGADVGC 

       790        800        810        820        830        840 
AVDTERMELR CGEGLVVWRE VSEWYDNYVF HPETPAVLAS AVQRAYEEEI CGIVPQNRLE 

       850        860        870        880        890        900 
MAMWRSSLVE LNLALAEGEA NLTVVVDKAD PSDYRGGVPG LLNKGKDIKV SWRSWGRSML 

       910        920        930        940        950        960 
WSVPEAPRRF MIGVEGGREC PFARRKTGVM TVAEFGIGLR TKVFMDLRQE LTTECDTGVM 

       970        980        990       1000       1010       1020 
GAAVKNGMAV HTDQSLWMKS IKNDTTVTIV ELIVTDLRNC TWPASHTIDN AGVVNSKLFL 

      1030       1040       1050       1060       1070       1080 
PASLAGPRST YNVIPGYAEQ VRGPWAHTPV RIKREECPGT RVTIDKACDK RGASVRSTTE 

      1090       1100       1110       1120       1130       1140 
SGKVIPEWCC RTCELPPVTY RTGTDCWYAM EIRPVHTQGG LVRSMVVADN GALLSEGGVP 

      1150       1160       1170       1180       1190       1200 
GVVALFVVLE LVIRRRPATG GTVIWGGIAI LALLVTGLVS VESLFRYLVA VGLVFQLELG 

      1210       1220       1230       1240       1250       1260 
PEAVAMVLLQ AVFEMRTCLL SGFVLRRSIT TREIVTVYFL LLVLEMGIPV KGLEHLWRWT 

      1270       1280       1290       1300       1310       1320 
DALAMGAIIF RACTAEGKTG IGLLLAAFMT QSDMNIIHDG LTAFLCVATT MAIWRYIRGQ 

      1330       1340       1350       1360       1370       1380 
GERKGLTWIV PLAGILGGEG SGVRLLAFWE LAASRGRRSF NEPMTVIGVM LTLASGMMRH 

      1390       1400       1410       1420       1430       1440 
TSQEAVCAMA LAAFLLLMLT LGTRKMQLLA EWSGNIEWNP ELTSEGGEVS LRVRQDALGN 

      1450       1460       1470       1480       1490       1500 
LHLTELEKEE RMMAFWLVVG LIASAFHWSG ILIVMGLWTI SEMLGSPRRT DLVFSGCSEG 

      1510       1520       1530       1540       1550       1560 
RSDSRPLDVK NGVYRIYTPG LLWGQRQIGV GYGAKGVLHT MWHVTRGAAL LVDGVAVGPY 

      1570       1580       1590       1600       1610       1620 
WADVREDVVC YGGAWSLESR WRGETVQVHA FPPGRAHETH QCQPGELILE NGRKMGAIPI 

      1630       1640       1650       1660       1670       1680 
DLAKGTSGSP IMNSQGEVVG LYGNGLKTND TYVSSIAQGE VEKSRPNLPQ SVVGTGWTAK 

      1690       1700       1710       1720       1730       1740 
GQITVLDMHP GSGKTHRVLP ELIRQCVERR LRTLVLAPTR VVLREMERAL SGKNVRFHSP 

      1750       1760       1770       1780       1790       1800 
AVTEQHANGA IVDVMCHATY VNRRLLPQGR QNWEVAIMDE AHWTDPHSIA ARGHLYSLAK 

      1810       1820       1830       1840       1850       1860 
ENRCAFVLMT ATPPGKSEPF PESNGAIASE ERQIPDGEWR DGFDWITEYE GRTAWFVPSI 

      1870       1880       1890       1900       1910       1920 
ARGGAIARAL RQRGKSVICL NSKTFDKEYS RVKDEKPDFV VTTDISEMGA NLDVTRVIDG 

      1930       1940       1950       1960       1970       1980 
RTNIKPEEVD GRIELTGTRR VTTASAAQRR GRVGRQGGRT DEYIYSGQCD DDDSGLVQWK 

      1990       2000       2010       2020       2030       2040 
EAQILLDNIT TARGPVATFY GPEQERMTET AGHYRLPEEK RKHFRHLLAQ CDFTPWLAWH 

      2050       2060       2070       2080       2090       2100 
VAANVASVTD RSWTWEGPEE NAVDENNGEL VTFRSPNGAE RTLRPVWRDA RMFREGRDIR 

      2110       2120       2130       2140       2150       2160 
EFVSYASGRR SVGDVLMGMS GVPALLRQRC TSAMDVFYTL MHEEPGSRAM RIGERDAPEA 

      2170       2180       2190       2200       2210       2220 
FLTAVEMLVL GLATLGVVWC FVVRTSVSRM VLGTLVLATS LIFLWAGGVG YGNMAGVALV 

      2230       2240       2250       2260       2270       2280 
FYTLLTVLQP ETGKQRSSDD NKLAYFLLTL CGLAGMVAAN EMGLLEKTKA DLAALFARDQ 

      2290       2300       2310       2320       2330       2340 
GETVRWGEWT NLDIQPARSW GTYVLVVSLF TPYMLHQLQT RIQQLVNSAV ASGAQAMRDL 

      2350       2360       2370       2380       2390       2400 
GGGTPFFGVA GHVLALGVAS LVGATPTSLI LGVGLAAFHL AIVVSGLEAE LTQRAHKVFF 

      2410       2420       2430       2440       2450       2460 
SAMVRNPMVD GDVINPFGDG EAKPALYERK LSLILALVLC LASVVMNRTF VAVTEAGAVG 

      2470       2480       2490       2500       2510       2520 
VAAAMQLLRP EMDVLWTMPV ACGMSGVVRG SLWGLLPLGH RLWLRTTGTR RGGSEGDTLG 

      2530       2540       2550       2560       2570       2580 
DMWKARLNSC TKEEFFAYRR AGVMETDREK ARELLKRGET NMGLAVSRGT SKLAWMEERG 

      2590       2600       2610       2620       2630       2640 
YVTLKGEVVD LGCGRGGWSY YAASRPAVMS VRAYTIGGKG HESPRMVTSL GWNLIKFRAG 

      2650       2660       2670       2680       2690       2700 
MDVFSMEPHR ADAILCDIGE SNPDAVVEGE RSRRVILLME QWKNRNPTAT CVFKVLAPYR 

      2710       2720       2730       2740       2750       2760 
PEVIEALHRF QLQWGGGLVR TPFSRNSTHE MYFSTAITGN IVNSVNIQSR KLLARFGDQR 

      2770       2780       2790       2800       2810       2820 
GPTRVPEIDL GVGTRSVVLA EDKVKEKDVM ERIQALKDQY CDTWHEDHEH PYRTWQYWGS 

      2830       2840       2850       2860       2870       2880 
YKTAATGSSA SLLNGVVKLL SWPWNAREDV VRMAMTDTTA FGQQRVFKDK VDTKAQEPQP 

      2890       2900       2910       2920       2930       2940 
GTKIIMRAVN DWLLERLVKK SRPRMCSREE FIAKVRSNAA LAAWSDEQNK WKSAREAVED 

      2950       2960       2970       2980       2990       3000 
PEFWSLVEAE RERHLQGRCA HCVYNMMGKR EKKLGEFGVA KGSRAIWYMW LGSRFLEFEA 

      3010       3020       3030       3040       3050       3060 
LGFLNEDHWA SRASSGAGVE GISLNYLGWH LKKLASLSGG LFYADDTAGW DTRITNADLD 

      3070       3080       3090       3100       3110       3120 
DEEQILRYMD GDHKKLAATV LRKAYHAKVV RVARPSREGG CVMDIITRRD QRGSGQVVTY 

      3130       3140       3150       3160       3170       3180 
ALNTITNIKV QLVRMMEGEG VIEVADSHNP RLLRVEKCVE EHGEERLSRM LVSGDDCVVR 

      3190       3200       3210       3220       3230       3240 
PVDDRFSKAL YFLNDMAKTR KDTGEWEPST GFASWEEVPF CSHHFHELVM KDGRALVVPC 

      3250       3260       3270       3280       3290       3300 
RDQDELVGRA RVSPGCGWSV RETACLSKAY GQMWLLSYFH RRDLRTLGFA ICSAVPVDWV 

      3310       3320       3330       3340       3350       3360 
PTGRTTWSIH ASGAWMTTED MLEVWNRVWI YDNPFMEDKT RVDEWRDTPY LPKSQDILCS 

      3370       3380       3390       3400       3410 
SLVGRGERAE WAKNIWGAVE KVRRMIGPEH YRDYLSSMDR HDLHWELKLE SSIF

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References

[1]"Sequence of the genes encoding the structural proteins of the low-virulence tick-borne flaviviruses Langat TP21 and Yelantsev."
Mandl C.W., Iacono-Connors L., Wallner G., Holzmann H., Kunz C., Heinz F.X.
Virology 185:891-895(1991) [PubMed: 1720591] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE OF 1-776.
[2]"Cloning and sequence analysis of the genes encoding the nonstructural proteins of Langat virus and comparative analysis with other flaviviruses."
Iacono-Connors L.C., Schmaljohn C.S.
Virology 188:875-880(1992) [PubMed: 1316684] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE OF 777-3414.
+Additional computationally mapped references.

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Cross-references

Sequence databases

M73835 Unassigned RNA. Translation: AAA02740.1. Different termination.
S35365 Genomic RNA. Translation: AAB22165.1.
PIRA42545.

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
1Z66NMR-A580-675[»]
2GG1NMR-A580-675[»]
SMRP29837. Positions 281-675.
ModBaseSearch...

Protein family/group databases

MEROPSS07.001.

Enzyme and pathway databases

BRENDA2.7.7.48. 322430.
3.4.21.91. 322430.

Family and domain databases

InterProIPR014001. DEAD-like_N.
IPR011492. DEAD_Flavivir.
IPR001650. DNA/RNA_helicase_C.
IPR002464. DNA/RNA_helicase_DEAH_CS.
IPR000069. Env_glycoprot_M_flavivir.
IPR011999. Flav_glyE_cen_dm.
IPR013754. Flav_glyE_dim.
IPR001122. Flavi_capsidC.
IPR001157. Flavi_NS1.
IPR000752. Flavi_NS2A.
IPR000487. Flavi_NS2B.
IPR000404. Flavi_NS4A.
IPR001528. Flavi_NS4B.
IPR002535. Flavi_propep.
IPR000336. Flv_glyE_Ig-like.
IPR014412. Gen_Poly_FLV.
IPR014021. Helicase_SF1/SF2_ATP-bd.
IPR001850. Peptidase_S7.
IPR000208. RNA_pol_flaviviral.
IPR007094. RNA_pol_PSvir.
IPR002877. rRNA_MeTrfase_RrmJ/FtsJ.
[Graphical view]
Gene3DG3DSA:2.60.98.10. Flav_glyE_dim. 1 hit.
G3DSA:2.60.40.350. Flv_glyE_Ig-like. 1 hit.
PfamPF01003. Flavi_capsid. 1 hit.
PF07652. Flavi_DEAD. 1 hit.
PF02832. Flavi_glycop_C. 1 hit.
PF00869. Flavi_glycoprot. 1 hit.
PF01004. Flavi_M. 1 hit.
PF00948. Flavi_NS1. 1 hit.
PF01005. Flavi_NS2A. 1 hit.
PF01002. Flavi_NS2B. 1 hit.
PF01350. Flavi_NS4A. 1 hit.
PF01349. Flavi_NS4B. 1 hit.
PF00972. Flavi_NS5. 1 hit.
PF01570. Flavi_propep. 1 hit.
PF01728. FtsJ. 1 hit.
PF00271. Helicase_C. 1 hit.
PF00949. Peptidase_S7. 1 hit.
[Graphical view]
PIRSFPIRSF003817. Gen_Poly_FLV. 1 hit.
ProDomPD001496. Flavi_NS1. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
PROSITEPS00690. DEAH_ATP_HELICASE. False negative.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50507. RDRP_SSRNA_POS. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry namePOLG_LANVT
AccessionPrimary (citable) accession number: P29837
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: February 1, 1994
Last modified: June 16, 2009
This is version 83 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectVirus (Virus annotation project)

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Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Peptidase families

Classification of peptidase families and list of entries

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents