P29829 (GBB2_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Guanine nucleotide-binding protein subunit beta-2

Gene names

Name:	Gbeta76C
Synonyms:	Gb76C, Gbe
ORF Names:	CG8770

Organism

Drosophila melanogaster (Fruit fly) [Reference proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Acalyptratae › Ephydroidea › Drosophilidae › Drosophilinae › Drosophilini › Drosophilina › Drosophiliti › Drosophila › Sophophora › melanogaster group › melanogaster subgroup

Protein attributes

Sequence length	346 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.
Subunit structure	G proteins are composed of 3 units, alpha, beta and gamma.
Tissue specificity	In the Drosophila compound eye.
Sequence similarities	Belongs to the WD repeat G protein beta family. Contains 7 WD repeats.

Ontologies

Keywords
Biological process	Sensory transduction Vision
Domain	Repeat WD repeat
Molecular function	Transducer
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	activation of phospholipase C activity Inferred from mutant phenotype PubMed 7759511. Source: FlyBase deactivation of rhodopsin mediated signaling Inferred from mutant phenotype PubMed 8015606. Source: FlyBase rhodopsin mediated signaling pathway Inferred from mutant phenotype PubMed 8015606. Source: FlyBase visual perception Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from direct assay PubMed 10608815. Source: FlyBase heterotrimeric G-protein complex Inferred from direct assay PubMed 7982946. Source: FlyBase rhabdomere Inferred from direct assay PubMed 10608815PubMed 7982946. Source: FlyBase
Molecular_function	GTPase activity Inferred from mutant phenotype PubMed 7982946PubMed 8015606. Source: FlyBase signal transducer activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Ggamma30A	Q9NFZ3	2	EBI-128499,EBI-2695634

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 346	346	Guanine nucleotide-binding protein subunit beta-2		PRO_0000127716
Regions
	Repeat	57 – 96	40	WD 1
	Repeat	99 – 138	40	WD 2
	Repeat	147 – 185	39	WD 3
	Repeat	188 – 227	40	WD 4
	Repeat	230 – 269	40	WD 5
	Repeat	274 – 313	40	WD 6
	Repeat	316 – 345	30	WD 7
Experimental info
	Sequence conflict	116	1	V → E in AAA73103. Ref.1
	Sequence conflict	136	1	S → P in AAA73103. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P29829 [UniParc]. Last modified March 15, 2004. Version 3. Checksum: E9B22D937EC3A707	FASTA	346	38,336
	10 20 30 40 50 60 MPKIDPETQK LYDEINGMIQ KFKDDQKSKA DCTLADKCGD MGDVPKIRFS SKKILKGHIN 70 80 90 100 110 120 KVNSVHFAGD SRHCVTGSLD GKLIIWDTWT ANKVQIIPLR SAWVMTVAFS PSGNFVACGG 130 140 150 160 170 180 MDNQCTVYDV NNRDASGVAK MVKELMGYEG FLSSCRFLDD GHLITGSGDM KICHWDLEKG 190 200 210 220 230 240 VKTMDFNGHA GDIAGLSLSP DMKTYITGSV DKTAKLWDVR EEGHKQMFFG HDMDVSSVCY 250 260 270 280 290 300 HPSGFGFASC SEDQTARMYD LRADQQIAQY EPPQKNTGFT SCALSTSGRY LMCGGIEGNV 310 320 330 340 HSWDTMKQRH TGTLSGHENR ITCISLCPNG MCLASTSWDQ QVRLWL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A G beta protein in the Drosophila compound eye is different from that in the brain." Yarfitz S., Niemi G.A., McConnell J.L., Fitch C.L., Hurley J.B. Neuron 7:429-438(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Eye.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[4]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Head.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M76593 mRNA. Translation: AAA73103.1. AE014296 Genomic DNA. Translation: AAF49124.1. AY118886 mRNA. Translation: AAM50746.1.
PIR	RGFFB. JU0269.
RefSeq	NP_523720.2. NM_078996.3.
UniGene	Dm.9760.
3D structure databases
ProteinModelPortal	P29829.
SMR	P29829. Positions 21-345.
ModBase	Search...
Protein-protein interaction databases
IntAct	P29829. 3 interactions.
MINT	MINT-938155.
STRING	7227.FBpp0074717.
Proteomic databases
PaxDb	P29829.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0074949; FBpp0074717; FBgn0004623.
GeneID	40148.
KEGG	dme:Dmel_CG8770.
Organism-specific databases
CTD	40148.
FlyBase	FBgn0004623. Gbeta76C.
Phylogenomic databases
eggNOG	COG2319.
GeneTree	ENSGT00550000074331.
InParanoid	P29829.
KO	K07972.
OMA	KGHINKV.
OrthoDB	EOG4SJ3W9.
PhylomeDB	P29829.
Gene expression databases
Bgee	P29829.
GermOnline	CG8770. Drosophila melanogaster.
Family and domain databases
Gene3D	2.130.10.10. 1 hit.
InterPro	IPR020472. G-protein_beta_WD-40_rep. IPR001632. Gprotein_B. IPR016346. Guanine_nucleotide-bd_bsu. IPR015943. WD40/YVTN_repeat-like_dom. IPR001680. WD40_repeat. IPR019775. WD40_repeat_CS. IPR017986. WD40_repeat_dom. [Graphical view]
Pfam	PF00400. WD40. 6 hits. [Graphical view]
PIRSF	PIRSF002394. GN-bd_beta. 1 hit.
PRINTS	PR00319. GPROTEINB. PR00320. GPROTEINBRPT.
SMART	SM00320. WD40. 7 hits. [Graphical view]
SUPFAM	SSF50978. WD40_like. 1 hit.
PROSITE	PS00678. WD_REPEATS_1. 1 hit. PS50082. WD_REPEATS_2. 4 hits. PS50294. WD_REPEATS_REGION. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	Gbeta76C. drosophila.
GenomeRNAi	40148.
NextBio	817248.

Entry information

Entry name

GBB2_DROME

Accession

Primary (citable) accession number: P29829
Secondary accession number(s): Q9VW29

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	March 15, 2004
Last modified:	May 1, 2013
This is version 112 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project