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Protein

Guanine nucleotide-binding protein subunit beta-2

Gene

Gbeta76C

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.

GO - Molecular functioni

  • protein heterodimerization activity Source: FlyBase
  • signal transducer activity Source: UniProtKB-KW

GO - Biological processi

  • activation of phospholipase C activity Source: FlyBase
  • deactivation of rhodopsin mediated signaling Source: FlyBase
  • G-protein coupled receptor signaling pathway Source: FlyBase
  • phototransduction Source: FlyBase
  • rhodopsin mediated signaling pathway Source: FlyBase
  • visual perception Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Transducer

Keywords - Biological processi

Sensory transduction, Vision

Enzyme and pathway databases

ReactomeiR-DME-1296041. Activation of G protein gated Potassium channels.
R-DME-4086398. Ca2+ pathway.
R-DME-418555. G alpha (s) signalling events.
R-DME-418594. G alpha (i) signalling events.
R-DME-6814122. Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding.
R-DME-997272. Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits.
SignaLinkiP29829.

Names & Taxonomyi

Protein namesi
Recommended name:
Guanine nucleotide-binding protein subunit beta-2
Gene namesi
Name:Gbeta76C
Synonyms:Gb76C, Gbe
ORF Names:CG8770
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0004623. Gbeta76C.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: FlyBase
  • heterotrimeric G-protein complex Source: FlyBase
  • plasma membrane Source: FlyBase
  • rhabdomere Source: FlyBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001277161 – 346Guanine nucleotide-binding protein subunit beta-2Add BLAST346

Proteomic databases

PaxDbiP29829.
PRIDEiP29829.

Expressioni

Tissue specificityi

In the Drosophila compound eye.

Gene expression databases

BgeeiFBgn0004623.
GenevisibleiP29829. DM.

Interactioni

Subunit structurei

G proteins are composed of 3 units, alpha, beta and gamma.

Binary interactionsi

WithEntry#Exp.IntActNotes
Ggamma30AQ9NFZ32EBI-128499,EBI-2695634

GO - Molecular functioni

  • protein heterodimerization activity Source: FlyBase

Protein-protein interaction databases

BioGridi65418. 4 interactors.
IntActiP29829. 5 interactors.
MINTiMINT-938155.
STRINGi7227.FBpp0074717.

Structurei

3D structure databases

ProteinModelPortaliP29829.
SMRiP29829.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati57 – 96WD 1Add BLAST40
Repeati99 – 138WD 2Add BLAST40
Repeati147 – 185WD 3Add BLAST39
Repeati188 – 227WD 4Add BLAST40
Repeati230 – 269WD 5Add BLAST40
Repeati274 – 313WD 6Add BLAST40
Repeati316 – 345WD 7Add BLAST30

Sequence similaritiesi

Belongs to the WD repeat G protein beta family.Curated
Contains 7 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiENOG410IU54. Eukaryota.
ENOG410XT5V. LUCA.
GeneTreeiENSGT00760000119239.
InParanoidiP29829.
KOiK07972.
OMAiWDTWTGN.
OrthoDBiEOG091G0A7T.
PhylomeDBiP29829.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR001632. Gprotein_B.
IPR016346. Guanine_nucleotide-bd_bsu.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PANTHERiPTHR19850. PTHR19850. 1 hit.
PfamiPF00400. WD40. 5 hits.
[Graphical view]
PRINTSiPR00319. GPROTEINB.
PR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 4 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P29829-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPKIDPETQK LYDEINGMIQ KFKDDQKSKA DCTLADKCGD MGDVPKIRFS
60 70 80 90 100
SKKILKGHIN KVNSVHFAGD SRHCVTGSLD GKLIIWDTWT ANKVQIIPLR
110 120 130 140 150
SAWVMTVAFS PSGNFVACGG MDNQCTVYDV NNRDASGVAK MVKELMGYEG
160 170 180 190 200
FLSSCRFLDD GHLITGSGDM KICHWDLEKG VKTMDFNGHA GDIAGLSLSP
210 220 230 240 250
DMKTYITGSV DKTAKLWDVR EEGHKQMFFG HDMDVSSVCY HPSGFGFASC
260 270 280 290 300
SEDQTARMYD LRADQQIAQY EPPQKNTGFT SCALSTSGRY LMCGGIEGNV
310 320 330 340
HSWDTMKQRH TGTLSGHENR ITCISLCPNG MCLASTSWDQ QVRLWL
Length:346
Mass (Da):38,336
Last modified:March 15, 2004 - v3
Checksum:iE9B22D937EC3A707
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti116V → E in AAA73103 (PubMed:1910788).Curated1
Sequence conflicti136S → P in AAA73103 (PubMed:1910788).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M76593 mRNA. Translation: AAA73103.1.
AE014296 Genomic DNA. Translation: AAF49124.1.
AY118886 mRNA. Translation: AAM50746.1.
PIRiJU0269. RGFFB.
RefSeqiNP_523720.2. NM_078996.3.
UniGeneiDm.9760.

Genome annotation databases

EnsemblMetazoaiFBtr0074949; FBpp0074717; FBgn0004623.
GeneIDi40148.
KEGGidme:Dmel_CG8770.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M76593 mRNA. Translation: AAA73103.1.
AE014296 Genomic DNA. Translation: AAF49124.1.
AY118886 mRNA. Translation: AAM50746.1.
PIRiJU0269. RGFFB.
RefSeqiNP_523720.2. NM_078996.3.
UniGeneiDm.9760.

3D structure databases

ProteinModelPortaliP29829.
SMRiP29829.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi65418. 4 interactors.
IntActiP29829. 5 interactors.
MINTiMINT-938155.
STRINGi7227.FBpp0074717.

Proteomic databases

PaxDbiP29829.
PRIDEiP29829.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0074949; FBpp0074717; FBgn0004623.
GeneIDi40148.
KEGGidme:Dmel_CG8770.

Organism-specific databases

CTDi40148.
FlyBaseiFBgn0004623. Gbeta76C.

Phylogenomic databases

eggNOGiENOG410IU54. Eukaryota.
ENOG410XT5V. LUCA.
GeneTreeiENSGT00760000119239.
InParanoidiP29829.
KOiK07972.
OMAiWDTWTGN.
OrthoDBiEOG091G0A7T.
PhylomeDBiP29829.

Enzyme and pathway databases

ReactomeiR-DME-1296041. Activation of G protein gated Potassium channels.
R-DME-4086398. Ca2+ pathway.
R-DME-418555. G alpha (s) signalling events.
R-DME-418594. G alpha (i) signalling events.
R-DME-6814122. Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding.
R-DME-997272. Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits.
SignaLinkiP29829.

Miscellaneous databases

ChiTaRSiGbeta76C. fly.
GenomeRNAii40148.
PROiP29829.

Gene expression databases

BgeeiFBgn0004623.
GenevisibleiP29829. DM.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR001632. Gprotein_B.
IPR016346. Guanine_nucleotide-bd_bsu.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PANTHERiPTHR19850. PTHR19850. 1 hit.
PfamiPF00400. WD40. 5 hits.
[Graphical view]
PRINTSiPR00319. GPROTEINB.
PR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 4 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGBB2_DROME
AccessioniPrimary (citable) accession number: P29829
Secondary accession number(s): Q9VW29
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: March 15, 2004
Last modified: November 30, 2016
This is version 141 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.