P29827 (CINA_STRGV) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lantibiotic cinnamycin

Alternative name(s):
Lanthiopeptin
Lantibiotic Ro 09-0198

Gene names

Name:	cinA
Synonyms:	rocA

Organism

Streptoverticillium griseoverticillatum

Taxonomic identifier

68215 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	78 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Can act as inhibitor of the enzyme phospholipase A2, and of the angiotensin-converting enzyme. Shows inhibitory activities against herpes simplex virus and immunopotentiating activities. Its antimicrobial activities are not very pronounced.
Post-translational modification	Maturation of lantibiotics involves the enzymic conversion of Thr, and Ser into dehydrated AA and the formation of thioether bonds with cysteine or the formation of dialkylamine bonds with lysine. This is followed by membrane translocation and cleavage of the modified precursor.
Sequence similarities	Belongs to the type B lantibiotic family.

Ontologies

Keywords
Molecular function	Antibiotic Antimicrobial Bacteriocin Lantibiotic
PTM	Hydroxylation Thioether bond
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological_process	cytolysis Inferred from electronic annotation. Source: UniProtKB-KW defense response to bacterium Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Propeptide

1 – 59

59

Potential

PRO_0000017148

Peptide

60 – 78

19

Lantibiotic cinnamycin Ref.2 Ref.3

PRO_0000017149

Amino acid modifications

Modified residue

74

1

(3R)-3-hydroxyaspartate

Cross-link

60 ↔ 77

Beta-methyllanthionine (Cys-Thr)

Cross-link

63 ↔ 73

Lanthionine (Ser-Cys)

Cross-link

64 ↔ 70

Beta-methyllanthionine (Cys-Thr)

Cross-link

65 ↔ 78

Lysinoalanine (Ser-Lys)

Secondary structure

1

.

.

.

78

Helix Strand Turn

Details...

Beta strand

65 – 72

8

Sequences

	Sequence		Length	Mass (Da)
	P29827 [UniParc]. Last modified April 1, 1993. Version 1. Checksum: 0ACDAE6BA54E5E7A	FASTA	78	8,205
	10 20 30 40 50 60 MTASILQQSV VDADFRAALL ENPAAFGASA AALPTPVEAQ DQASLDFWTK DIAATEAFAC 70 RQSCSFGPFT FVCDGNTK

References

[1]	"Prepeptide sequence of cinnamycin (Ro 09-0198): the first structural gene of a duramycin-type lantibiotic." Kaletta C., Entian K.-D., Jung G. Eur. J. Biochem. 199:411-415(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: MAR 164C-MY6.
[2]	"Duramycins B and C, two new lanthionine containing antibiotics as inhibitors of phospholipase A2. Structural revision of duramycin and cinnamycin." Fredenhagen A., Fendrich G., Marki F., Marki W., Gruner J., Raschdorf F., Peter H.H. J. Antibiot. 43:1403-1412(1990) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 60-78.
[3]	"Lanthiopeptin, a new peptide antibiotic. Production, isolation and properties of lanthiopeptin." Naruse N., Tenmyo O., Tomita K., Konishi M., Miyaki T., Kawaguchi H., Fukase K., Wakamiya T., Shiba T. J. Antibiot. 42:837-845(1989) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 60-78, HYDROXYLATION AT ASP-74.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X58545 Genomic DNA. Translation: CAA41436.1.

PIR

EWSMCN. A45767.
EWSMYG. S17181.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2DDE	NMR	-	A	60-78	[»]

ProteinModelPortal

P29827.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

ProtoNet

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Entry information

Entry name

CINA_STRGV

Accession

Primary (citable) accession number: P29827

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	April 3, 2013
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program