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P29821

- VP7_ROTCC

UniProt

P29821 - VP7_ROTCC

Protein

Outer capsid glycoprotein VP7

Gene
N/A
Organism
Rotavirus A (strain Chicken/Ireland/Ch2/1979 G7-Px[x]-Ix-Rx-Cx-M4-Ax-Nx-Tx-Ex-Hx) (RV-A) (Rotavirus A (strain Ch-2))
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 68 (01 Oct 2014)
      Sequence version 1 (01 Apr 1993)
      Previous versions | rss
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    Functioni

    Outer capsid protein involved in attachment and possibly entry into the host epithelial cell. It is subsequently lost, together with VP4, following virus entry into the host cell. The outer layer contains 780 copies of VP7, grouped as 260 trimers. Rotavirus attachment and entry into the host cell probably involves multiple sequential contacts between the outer capsid proteins VP4 and VP7, and the cell receptors. In integrin-dependent strains, VP7 seems to essentially target the integrin heterodimers ITGAX/ITGB2 and ITGA5/ITGB3 at a postbinding stage, once the initial attachment by VP4 has been achieved By similarity.By similarity

    GO - Biological processi

    1. viral process Source: UniProtKB-KW

    Keywords - Biological processi

    Host-virus interaction

    Keywords - Ligandi

    Calcium

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Outer capsid glycoprotein VP7
    OrganismiRotavirus A (strain Chicken/Ireland/Ch2/1979 G7-Px[x]-Ix-Rx-Cx-M4-Ax-Nx-Tx-Ex-Hx) (RV-A) (Rotavirus A (strain Ch-2))
    Taxonomic identifieri31589 [NCBI]
    Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
    Virus hostiGallus gallus (Chicken) [TaxID: 9031]

    Subcellular locationi

    Virion By similarity. Host endoplasmic reticulum lumen Curated
    Note: Immature double-layered particles assembled in the cytoplasm bud across the membrane of the endoplasmic reticulum, acquiring during this process a transient lipid membrane that is modified with the ER resident viral glycoproteins NSP4 and VP7; these enveloped particles also contain VP4. As the particles move towards the interior of the ER cisternae, the transient lipid membrane and the non-structural protein NSP4 are lost, while the virus surface proteins VP4 and VP7 rearrange to form the outermost virus protein layer, yielding mature infectious triple-layered particles By similarity.By similarity

    GO - Cellular componenti

    1. host cell endoplasmic reticulum lumen Source: UniProtKB-SubCell
    2. T=13 icosahedral viral capsid Source: UniProtKB-KW
    3. viral outer capsid Source: UniProtKB-KW

    Keywords - Cellular componenti

    Capsid protein, Host endoplasmic reticulum, Outer capsid protein, T=13 icosahedral capsid protein, Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 5353Sequence AnalysisAdd
    BLAST
    Chaini54 – 329276Outer capsid glycoprotein VP7Sequence AnalysisPRO_0000149588Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi72 – 721N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi193 – 1931N-linked (GlcNAc...); by hostSequence Analysis

    Post-translational modificationi

    The N-terminus is blocked possibly by pyroglutamic acid.By similarity
    N-glycosylated.By similarity
    Intramolecular disulfide bonds.By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Interactioni

    Subunit structurei

    Homotrimer; in the presence of calcium By similarity. Acquisition of the capsid outer layer requires a high calcium concentration inside the endoplasmic reticulum. Following cell entry, the low calcium concentration in the cytoplasm is probably responsible for the solubilization of the outer layer. Interacts with host integrin heterodimers ITGAX/ITGB2 and ITGA5/ITGB3 By similarity.By similarity

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the rotavirus VP7 family.Curated

    Keywords - Domaini

    Signal

    Family and domain databases

    InterProiIPR001963. VP7.
    [Graphical view]
    PfamiPF00434. VP7. 1 hit.
    [Graphical view]
    ProDomiPD000191. VP7. 1 hit.
    [Graphical view] [Entries sharing at least one domain]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P29821-1 [UniParc]FASTAAdd to Basket

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    MYSTKCTNFF LEIIFYVIFC TLFLLVLEKM SKLLSWIVIV CLFVFAISSK    50
    CSAQNYGINV PITGSMDVVL ANSTQDQIGL TSTLCIYYPK AADTEIADPE 100
    WKATVTQLLL TKGWPTTSVY LNEYQDLVTF SNDPKLYCDY NIVLAHYTND 150
    VALDISELAE FLLYEWLCNP MDVTLYYYQQ TSEPNKWIAM GTNCTIKVCP 200
    LNTQTLGIGC QTTNTDTFEI LTMSEKLAII DVVDGVNHKV DYTVATCKIN 250
    NCIRLNPREN VAIIQVGGPE VLDISENPMV IPKVSRMTRM NWKKWWQVFY 300
    TIVDYINTII TTMSKRSRSL DVSSYYYRV 329
    Length:329
    Mass (Da):37,617
    Last modified:April 1, 1993 - v1
    Checksum:i88C705DAD2C33792
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X56784 mRNA. Translation: CAA40104.1.
    PIRiA41703. VGXRC2.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X56784 mRNA. Translation: CAA40104.1 .
    PIRi A41703. VGXRC2.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR001963. VP7.
    [Graphical view ]
    Pfami PF00434. VP7. 1 hit.
    [Graphical view ]
    ProDomi PD000191. VP7. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence of the VP7 gene of chicken rotavirus Ch2 strain of serotype 7 rotavirus."
      Nishikawa K., Hoshino Y., Gorziglia M.
      Virology 185:853-856(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].

    Entry informationi

    Entry nameiVP7_ROTCC
    AccessioniPrimary (citable) accession number: P29821
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1993
    Last sequence update: April 1, 1993
    Last modified: October 1, 2014
    This is version 68 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    In group A rotaviruses, VP7 defines the G serotype.

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3