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P29821

- VP7_ROTCC

UniProt

P29821 - VP7_ROTCC

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Protein

Outer capsid glycoprotein VP7

Gene
N/A
Organism
Rotavirus A (strain Chicken/Ireland/Ch2/1979 G7-Px[x]-Ix-Rx-Cx-M4-Ax-Nx-Tx-Ex-Hx) (RV-A) (Rotavirus A (strain Ch-2))
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at transcript leveli

Functioni

Outer capsid protein involved in attachment and possibly entry into the host epithelial cell. It is subsequently lost, together with VP4, following virus entry into the host cell. The outer layer contains 780 copies of VP7, grouped as 260 trimers. Rotavirus attachment and entry into the host cell probably involves multiple sequential contacts between the outer capsid proteins VP4 and VP7, and the cell receptors. In integrin-dependent strains, VP7 seems to essentially target the integrin heterodimers ITGAX/ITGB2 and ITGA5/ITGB3 at a postbinding stage, once the initial attachment by VP4 has been achieved (By similarity).By similarity

GO - Biological processi

  1. viral process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Host-virus interaction

Keywords - Ligandi

Calcium

Names & Taxonomyi

Protein namesi
Recommended name:
Outer capsid glycoprotein VP7
OrganismiRotavirus A (strain Chicken/Ireland/Ch2/1979 G7-Px[x]-Ix-Rx-Cx-M4-Ax-Nx-Tx-Ex-Hx) (RV-A) (Rotavirus A (strain Ch-2))
Taxonomic identifieri31589 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
Virus hostiGallus gallus (Chicken) [TaxID: 9031]

Subcellular locationi

Virion By similarity. Host endoplasmic reticulum lumen Curated
Note: Immature double-layered particles assembled in the cytoplasm bud across the membrane of the endoplasmic reticulum, acquiring during this process a transient lipid membrane that is modified with the ER resident viral glycoproteins NSP4 and VP7; these enveloped particles also contain VP4. As the particles move towards the interior of the ER cisternae, the transient lipid membrane and the non-structural protein NSP4 are lost, while the virus surface proteins VP4 and VP7 rearrange to form the outermost virus protein layer, yielding mature infectious triple-layered particles (By similarity).By similarity

GO - Cellular componenti

  1. host cell endoplasmic reticulum Source: UniProtKB-KW
  2. T=13 icosahedral viral capsid Source: UniProtKB-KW
  3. viral outer capsid Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Capsid protein, Host endoplasmic reticulum, Outer capsid protein, T=13 icosahedral capsid protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 5353Sequence AnalysisAdd
BLAST
Chaini54 – 329276Outer capsid glycoprotein VP7Sequence AnalysisPRO_0000149588Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi72 – 721N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi193 – 1931N-linked (GlcNAc...); by hostSequence Analysis

Post-translational modificationi

The N-terminus is blocked possibly by pyroglutamic acid.By similarity
N-glycosylated.By similarity
Intramolecular disulfide bonds.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Subunit structurei

Homotrimer; in the presence of calcium (By similarity). Acquisition of the capsid outer layer requires a high calcium concentration inside the endoplasmic reticulum. Following cell entry, the low calcium concentration in the cytoplasm is probably responsible for the solubilization of the outer layer. Interacts with host integrin heterodimers ITGAX/ITGB2 and ITGA5/ITGB3 (By similarity).By similarity

Family & Domainsi

Sequence similaritiesi

Belongs to the rotavirus VP7 family.Curated

Keywords - Domaini

Signal

Family and domain databases

InterProiIPR001963. VP7.
[Graphical view]
PfamiPF00434. VP7. 1 hit.
[Graphical view]
ProDomiPD000191. VP7. 1 hit.
[Graphical view] [Entries sharing at least one domain]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P29821-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MYSTKCTNFF LEIIFYVIFC TLFLLVLEKM SKLLSWIVIV CLFVFAISSK
60 70 80 90 100
CSAQNYGINV PITGSMDVVL ANSTQDQIGL TSTLCIYYPK AADTEIADPE
110 120 130 140 150
WKATVTQLLL TKGWPTTSVY LNEYQDLVTF SNDPKLYCDY NIVLAHYTND
160 170 180 190 200
VALDISELAE FLLYEWLCNP MDVTLYYYQQ TSEPNKWIAM GTNCTIKVCP
210 220 230 240 250
LNTQTLGIGC QTTNTDTFEI LTMSEKLAII DVVDGVNHKV DYTVATCKIN
260 270 280 290 300
NCIRLNPREN VAIIQVGGPE VLDISENPMV IPKVSRMTRM NWKKWWQVFY
310 320
TIVDYINTII TTMSKRSRSL DVSSYYYRV
Length:329
Mass (Da):37,617
Last modified:April 1, 1993 - v1
Checksum:i88C705DAD2C33792
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X56784 mRNA. Translation: CAA40104.1.
PIRiA41703. VGXRC2.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X56784 mRNA. Translation: CAA40104.1 .
PIRi A41703. VGXRC2.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR001963. VP7.
[Graphical view ]
Pfami PF00434. VP7. 1 hit.
[Graphical view ]
ProDomi PD000191. VP7. 1 hit.
[Graphical view ] [Entries sharing at least one domain ]
ProtoNeti Search...

Publicationsi

  1. "Sequence of the VP7 gene of chicken rotavirus Ch2 strain of serotype 7 rotavirus."
    Nishikawa K., Hoshino Y., Gorziglia M.
    Virology 185:853-856(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiVP7_ROTCC
AccessioniPrimary (citable) accession number: P29821
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: October 29, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

In group A rotaviruses, VP7 defines the G serotype.

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3