P29814 (NTP1_AMEPV) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nucleoside triphosphatase I
EC=3.6.1.15

Alternative name(s):
NPH-I
Nucleoside triphosphate phosphohydrolase I
Short name=NPH I

Gene names

Name:	NPH1
Ordered Locus Names:	AMV192
ORF Names:	G6

Organism

Amsacta moorei entomopoxvirus (AmEPV) [Reference proteome]

Taxonomic identifier

28321 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Poxviridae › Entomopoxvirinae › Betaentomopoxvirus

Virus host

Amsacta [TaxID: 340055]

Protein attributes

Sequence length	648 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA-dependent ATPase required for providing the needed energy to achieve the termination of early transcripts. Acts in concert with the RAP94 subunit of the virion RNA polymerase and the capping enzyme/VTF to catalyze release of UUUUUNU-containing nascent RNA from the elongation complex. NPH-I must bind ssDNA in order to exhibit ATPase activity By similarity.
Catalytic activity	NTP + H2O = NDP + phosphate.
Subunit structure	Monomer. Interacts (via C-terminus) with RAP94 (via N-terminus). Interacts with the cap-specific mRNA (nucleoside-2'-O-)-methyltransferase By similarity.
Subcellular location	Virion By similarity. Note: Virion core enzyme By similarity.
Sequence similarities	Belongs to the helicase family. NPH I subfamily. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.

Ontologies

Keywords
Biological process	Transcription
Cellular component	Virion
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	virion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA binding Inferred from electronic annotation. Source: UniProtKB-KW helicase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 648	648	Nucleoside triphosphatase I		PRO_0000099099
Regions
	Domain	48 – 212	165	Helicase ATP-binding
	Domain	378 – 541	164	Helicase C-terminal
	Nucleotide binding	61 – 68	8	ATP By similarity
	Region	467 – 533	67	Binding to the cap-specific mRNA (nucleoside-2'-O-)-methyltransferase By similarity
	Motif	150 – 153	4	DEXH box

Sequences

	Sequence		Length	Mass (Da)
	P29814 [UniParc]. Last modified February 1, 1996. Version 2. Checksum: 25BE863C0B8728AA	FASTA	648	76,109
	10 20 30 40 50 60 MFALDSIVGK HINYALDKTQ HLPNKIMNNI TNTEITLQDY QYFVSRIFIG LKNLNSMLLF 70 80 90 100 110 120 WDTGMGKTLT AVYIIKYIKE LFPRWIILIF IKKSLYIDPW LNTIRSYISD TSNIKFIYYD 130 140 150 160 170 180 SSSSLDKFNN IYRSIESSLN KKSRLLIIID EVHKLISRTV KKDNNERNFT PIYKKLIKLA 190 200 210 220 230 240 NFENNKILCM SATPVTNNIS EFNNLIGLLR PNVMNIKEEY INNGKLINFK ELRETLLAIC 250 260 270 280 290 300 SYKRLIEADS LTETNYIDGY AKKNIFYHNI IMSDEQSKLY NMAEKYDYKT ELGGLKTMRR 310 320 330 340 350 360 LISSFAFYDL KIKGDLDNIE YNDMIKRKLA EFSEFTKNIN FSESFIESFK NDNIKIKTNL 370 380 390 400 410 420 PITDINNYNI LYQYSCKYIE TCKIILNSRG KVLIFEPLVN FEGISSLKCY FNCFNISYIE 430 440 450 460 470 480 YSSKTLKTRD NELNEYNNYE NNNGKKVKVC IFSYAGSEGI SFKCINDIII LDMPWNESEL 490 500 510 520 530 540 KQIIGRSIRL NSHKDLPQEY RYVNVHFLIS YTNNRKSVDK EILDIIKDKQ GKINVIFDLL 550 560 570 580 590 600 KSSSIESIHN TYKYIEPAEN EIIFDTIRKT RMKEMNVSNV IINIKLYPIS YCKDYDRATI 610 620 630 640 LKGLLNKDTN IVYKDNTAVA KLMIDKDNIP IFIIENDTLI YIADDYYE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genomic sequence of the Amsacta moorei entomopoxvirus: analysis and comparison with other poxviruses." Bawden A.L., Glassberg K.J., Diggans J., Shaw R., Farmerie W., Moyer R.W. Virology 274:120-139(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"Identification of an Amsacta spheroidin-like protein within the occlusion bodies of Choristoneura entomopoxviruses." Hall R.L., Moyer R.W. Virology 192:179-187(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-486.
[3]	"Identification, cloning, and sequencing of a fragment of Amsacta moorei entomopoxvirus DNA containing the spheroidin gene and three vaccinia virus-related open reading frames." Hall R.L., Moyer R.W. J. Virol. 65:6516-6527(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 486-648.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF250284 Genomic DNA. Translation: AAG02898.1. M77182 Genomic DNA. Translation: AAA42384.1.
PIR	NPVZAM. F41561.
RefSeq	NP_064974.1. NC_002520.1.
3D structure databases
ProteinModelPortal	P29814.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1494782.
Phylogenomic databases
ProtClustDB	CLSP2509799.
Family and domain databases
InterPro	IPR014001. Helicase_ATP-bd. IPR001650. Helicase_C. IPR013676. NPHI_C. IPR000330. SNF2_N. [Graphical view]
Pfam	PF00271. Helicase_C. 1 hit. PF08469. NPHI_C. 1 hit. PF00176. SNF2_N. 1 hit. [Graphical view]
SMART	SM00487. DEXDc. 1 hit. SM00490. HELICc. 1 hit. [Graphical view]
PROSITE	PS51192. HELICASE_ATP_BIND_1. 1 hit. PS51194. HELICASE_CTER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NTP1_AMEPV

Accession

Primary (citable) accession number: P29814

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	February 1, 1996
Last modified:	April 3, 2013
This is version 68 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program