Reviewed, UniProtKB/Swiss-Prot P29811 (CYSC2_PSEAE)

Names and origin

Protein names	Recommended name:     Probable adenylyl-sulfate kinase     EC=2.7.1.25 Alternative name(s):     APS kinase     Adenosine-5'-phosphosulfate kinase     ATP adenosine-5'-phosphosulfate 3'-phosphotransferase
Organism	Pseudomonas aeruginosa
Taxonomic identifier	287 [NCBI]
Taxonomic lineage	Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	214 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the synthesis of activated sulfate. HAMAP MF_00065
Catalytic activity	ATP + adenylyl sulfate = ADP + 3'-phosphoadenylyl sulfate. HAMAP MF_00065
Pathway	Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 2/3. HAMAP MF_00065
Sequence similarities	Belongs to the APS kinase family.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	sulfate assimilation Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP adenylylsulfate kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 214	214	Probable adenylyl-sulfate kinase HAMAP MF_00065		PRO_0000105914
Regions
	Nucleotide binding	13 – 20	8	ATP Potential
Sites
	Active site	87	1	Phosphoserine intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P29811-1 [UniParc]. Last modified April 1, 1993. Version 1. Checksum: B0C207AC0DE9949E	FASTA	214	23,922
	10 20 30 40 50 60 MIEHPGFVLW FTGLSGAGKT TIAVALENQL RARGIRIERL DGDTVRQSLT KDLGFSKEDR 70 80 90 100 110 120 DKNIERVTFV AKLLSRNNVA VLSSFISPYA ATRDHVRGET TNFIEVFVDA PLETCIERDV 130 140 150 160 170 180 KGMYKKAIAG EIPNFTGISD PYEAPANPDI HVKTHEQTLE ESVQTIIRRS SHGWNRTNTF 190 200 210 PLKSRPNPPH RHKSKSSRAG EPFISPVFVL SIIA

References

[1]

"Nucleotide sequence of the aacC3 gene, a gentamicin resistance determinant encoding aminoglycoside-(3)-N-acetyltransferase III expressed in Pseudomonas aeruginosa but not in Escherichia coli." Vliegenthart J.S., Ketelaar-Van Gaalen P.A.G., van de Klundert J.A.M. Antimicrob. Agents Chemother. 35:892-897(1991) [PubMed: 1649572] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: PST-I.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X55652 Genomic DNA. Translation: CAA39183.1.
PIR	S18729.
3D structure databases
HSSP	HSSP built from PDB template 1M7G based on UniProtKB Q12657.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.7.1.25. 354.
Family and domain databases
HAMAP	MF_00065. [Tree]
InterPro	IPR002891. APS_kinase_C. [Graphical view]
Pfam	PF01583. APS_kinase. 1 hit. [Graphical view]
ProDom	PD002350. APS_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00455. apsK. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CYSC2_PSEAE

Accession

Primary (citable) accession number: P29811

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	January 20, 2009
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)