P29811 (CYSC_PSEAI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Probable adenylyl-sulfate kinase EC=2.7.1.25 Alternative name(s): APS kinase ATP adenosine-5'-phosphosulfate 3'-phosphotransferase Adenosine-5'-phosphosulfate kinase
Organism	Pseudomonas aeruginosa
Taxonomic identifier	287 [NCBI]
Taxonomic lineage	Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	214 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the synthesis of activated sulfate. HAMAP MF_00065
Catalytic activity	ATP + adenylyl sulfate = ADP + 3'-phosphoadenylyl sulfate. HAMAP MF_00065
Pathway	Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 2/3. HAMAP MF_00065
Sequence similarities	Belongs to the APS kinase family.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	sulfate assimilation Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW adenylylsulfate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 214	214	Probable adenylyl-sulfate kinase HAMAP MF_00065		PRO_0000105914
Regions
	Nucleotide binding	13 – 20	8	ATP Potential
Sites
	Active site	87	1	Phosphoserine intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P29811 [UniParc]. Last modified April 1, 1993. Version 1. Checksum: B0C207AC0DE9949E	FASTA	214	23,922
	10 20 30 40 50 60 MIEHPGFVLW FTGLSGAGKT TIAVALENQL RARGIRIERL DGDTVRQSLT KDLGFSKEDR 70 80 90 100 110 120 DKNIERVTFV AKLLSRNNVA VLSSFISPYA ATRDHVRGET TNFIEVFVDA PLETCIERDV 130 140 150 160 170 180 KGMYKKAIAG EIPNFTGISD PYEAPANPDI HVKTHEQTLE ESVQTIIRRS SHGWNRTNTF 190 200 210 PLKSRPNPPH RHKSKSSRAG EPFISPVFVL SIIA

References

[1]

"Nucleotide sequence of the aacC3 gene, a gentamicin resistance determinant encoding aminoglycoside-(3)-N-acetyltransferase III expressed in Pseudomonas aeruginosa but not in Escherichia coli." Vliegenthart J.S., Ketelaar-Van Gaalen P.A.G., van de Klundert J.A.M. Antimicrob. Agents Chemother. 35:892-897(1991) [PubMed: 1649572] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: PST-I.

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Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X55652 Genomic DNA. Translation: CAA39183.1.
PIR	S18729.
3D structure databases
ProteinModelPortal	P29811.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
HAMAP	MF_00065. Adenylyl_sulf_kinase. [Tree]
InterPro	IPR002891. APS_kinase_C. [Graphical view]
Pfam	PF01583. APS_kinase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00455. ApsK. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CYSC_PSEAI

Accession

Primary (citable) accession number: P29811

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	November 16, 2011
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program