Reviewed, UniProtKB/Swiss-Prot P29806 (AADB2_KLEPN)

Names and origin

Protein names

Recommended name:
    2''-aminoglycoside nucleotidyltransferase
    EC=2.7.7.46
Alternative name(s):
    AAD(2'')
    Gentamicin 2''-nucleotidyltransferase
    Gentamicin resistance protein

Gene names

Name:

aadB

Encoded on

Plasmid pLST1000

Organism

Klebsiella pneumoniae

Taxonomic identifier

573 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Klebsiella

Protein attributes

Sequence length	177 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Predicted.

General annotation (Comments)

Function	This enzyme confers resistance to kanamycin, gentamicin, dibekacin, sisomicin, neomycin and tobramycin by adenylating the 2''-hydroxyl group of these antibiotics.
Catalytic activity	Nucleoside triphosphate + gentamicin = diphosphate + 2''-nucleotidylgentamicin.

Ontologies

Keywords
Biological process	Antibiotic resistance
Molecular function	Nucleotidyltransferase Transferase
Technical term	Plasmid
Gene Ontology (GO)
Biological process	response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	aminoglycoside 2''-nucleotidyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 177	177	2''-aminoglycoside nucleotidyltransferase		PRO_0000068558

Sequences

	Sequence		Length	Mass (Da)
	P29806-1 [UniParc]. Last modified April 1, 1993. Version 1. Checksum: F941A0785D77E719	FASTA	177	19,861
	10 20 30 40 50 60 MDTTQVTLIH QILAAADERN LPLWIGGGWA IDARLGRVTR KHDDIDLTFP GERRGELEAM 70 80 90 100 110 120 VEMLGGRVTE ELDYGFLAEI GDELLDCEPA WWADEAYEIA EAPQGSCPEA AEGVIAGRPV 130 140 150 160 170 RCNSWEAIIW DYFYYADEVP PVDWPTKHIE SYRLACTSLG AEKVEVLRAA FRSRYAA

References

[1]	Zieg J., Jiang H., McCabe F., O'Brien T. Submitted (FEB-1992) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	X64369 Genomic DNA. Translation: CAA45722.1.
PIR	XNKBLS. S19940.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.7.7.46. 758.
Family and domain databases
InterPro	IPR019646. Aminoglyc_AdlTrfase. [Graphical view]
Pfam	PF10706. Aminoglyc_resit. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AADB2_KLEPN

Accession

Primary (citable) accession number: P29806

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	June 16, 2009
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)