##gff-version 3
P29803	UniProtKB	Transit peptide	1	27	.	.	.	Note=Mitochondrion;Ontology_term=ECO:0000250;evidence=ECO:0000250	
P29803	UniProtKB	Chain	28	388	.	.	.	ID=PRO_0000020447;Note=Pyruvate dehydrogenase E1 component subunit alpha%2C testis-specific form%2C mitochondrial	
P29803	UniProtKB	Modified residue	291	291	.	.	.	Note=Phosphoserine%3B by PDK1%2C PDK2%2C PDK3 and PDK4;Ontology_term=ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:16436377,ECO:0000269|PubMed:31536960;Dbxref=PMID:16436377,PMID:31536960	
P29803	UniProtKB	Modified residue	293	293	.	.	.	Note=Phosphoserine;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:31536960;Dbxref=PMID:31536960	
P29803	UniProtKB	Modified residue	298	298	.	.	.	Note=Phosphoserine%3B by PDK3;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:16436377;Dbxref=PMID:16436377	
P29803	UniProtKB	Natural variant	227	227	.	.	.	ID=VAR_087365;Note=In SPGF70%3B uncertain significance. M->V;Ontology_term=ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:29581481,ECO:0000269|PubMed:35172124;Dbxref=dbSNP:rs200969445,PMID:29581481,PMID:35172124	
P29803	UniProtKB	Natural variant	376	376	.	.	.	ID=VAR_034359;Note=R->G;Dbxref=dbSNP:rs17024795	
P29803	UniProtKB	Mutagenesis	230	230	.	.	.	Note=Slightly reduces enzyme activity. S->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:16436377;Dbxref=PMID:16436377	
P29803	UniProtKB	Mutagenesis	291	291	.	.	.	Note=Strongly reduces enzyme activity. Increases enzyme activity in stem cells. S->A;Ontology_term=ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:16436377,ECO:0000269|PubMed:31536960;Dbxref=PMID:16436377,PMID:31536960	
P29803	UniProtKB	Mutagenesis	291	291	.	.	.	Note=Abolishes enzyme activity. Increases neuronal cell death in response to glutamate excitotoxicity. S->E%2CD;Ontology_term=ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:16436377,ECO:0000269|PubMed:31536960;Dbxref=PMID:16436377,PMID:31536960	
P29803	UniProtKB	Mutagenesis	293	293	.	.	.	Note=Increases enzyme activity in stem cells. S->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:31536960;Dbxref=PMID:31536960	
P29803	UniProtKB	Mutagenesis	293	293	.	.	.	Note=Abolishes enzyme activity. Increases neuronal cell death in response to glutamate excitotoxicity. S->D;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:31536960;Dbxref=PMID:31536960	
P29803	UniProtKB	Mutagenesis	298	298	.	.	.	Note=Slightly reduces enzyme activity. S->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:16436377;Dbxref=PMID:16436377	
P29803	UniProtKB	Sequence conflict	127	127	.	.	.	Note=L->P;Ontology_term=ECO:0000305;evidence=ECO:0000305