Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Guanine nucleotide-binding protein G(s) subunit alpha

Gene

GNAS

Organism
Sus scrofa (Pig)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Guanine nucleotide-binding proteins (G proteins) function as transducers in numerous signaling pathways controlled by G protein-coupled receptors (GPCRs). Signaling involves the activation of adenylyl cyclases, resulting in increased levels of the signaling molecule cAMP. GNAS functions downstream of several GPCRs, including beta-adrenergic receptors. Stimulates the Ras signaling pathway via RAPGEF2.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi54MagnesiumBy similarity1
Metal bindingi189MagnesiumBy similarity1
Binding sitei369GTP; via amide nitrogenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi47 – 55GTPBy similarity9
Nucleotide bindingi182 – 189GTPBy similarity8
Nucleotide bindingi208 – 212GTPBy similarity5
Nucleotide bindingi277 – 280GTPBy similarity4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransducer
LigandGTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Guanine nucleotide-binding protein G(s) subunit alpha
Alternative name(s):
Adenylate cyclase-stimulating G alpha protein
Gene namesi
Name:GNAS
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
Proteomesi
  • UP000008227 Componenti: Unplaced

Subcellular locationi

  • Cell membrane By similarity; Lipid-anchor By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved
ChainiPRO_00002037242 – 397Guanine nucleotide-binding protein G(s) subunit alphaAdd BLAST396

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-palmitoyl glycineBy similarity1
Lipidationi3S-palmitoyl cysteineBy similarity1

Keywords - PTMi

Lipoprotein, Palmitate

Proteomic databases

PeptideAtlasiP29797.
PRIDEiP29797.

Interactioni

Subunit structurei

Heterotrimeric G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site (By similarity). Interacts with CRY1; the interaction may block GPCR-mediated regulation of cAMP concentrations. Interacts with ADCY6 and stimulates its adenylyl cyclase activity (By similarity). Interacts with ADCY2 and ADCY5 (By similarity). Stimulates the ADCY5 adenylyl cyclase activity (By similarity).By similarity

GO - Molecular functioni

Structurei

3D structure databases

ProteinModelPortaliP29797.
SMRiP29797.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-alpha family. G(s) subfamily.Curated

Phylogenomic databases

HOGENOMiHOG000038729.
HOVERGENiHBG063184.
InParanoidiP29797.

Family and domain databases

CDDicd00066. G-alpha. 1 hit.
Gene3Di1.10.400.10. 1 hit.
InterProiView protein in InterPro
IPR000367. Gprotein_alpha_S.
IPR001019. Gprotein_alpha_su.
IPR011025. GproteinA_insert.
IPR027417. P-loop_NTPase.
PANTHERiPTHR10218. PTHR10218. 1 hit.
PfamiView protein in Pfam
PF00503. G-alpha. 1 hit.
PRINTSiPR00318. GPROTEINA.
PR00443. GPROTEINAS.
SMARTiView protein in SMART
SM00275. G_alpha. 1 hit.
SUPFAMiSSF47895. SSF47895. 1 hit.
SSF52540. SSF52540. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P29797-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGCLGNSKTE DQRNEEKAQR EANKKIEKQL QKDKQVYRAT HRLLLLGAGE
60 70 80 90 100
SGKSTIVKQM RILHVNGFNG DEKATKVQDI KNNLKEAIET IVAAMSNLVP
110 120 130 140 150
PVELANPENQ FRVDYILSVM NVPDFDFPPE FYEHAKALWE DEGVRACYER
160 170 180 190 200
SNEYQLIDCA QYFLDKIDVI KQDDYVPSDQ DLLRCRVLTS GIFETKFQVD
210 220 230 240 250
KVNFHMFDVG GQRDERRKWI QCFNDVTAII FVVASSSYNM VIREDNQTNR
260 270 280 290 300
LQEALNLFKS IWNNRWLRTI SVILFLNKQD LLAEKVLAGK SKIELFVLDD
310 320 330 340 350
RLFQERPFSF IEDYFPEFAR YTTPEDATPE PGEDPRVTRA KYFIRDEFLR
360 370 380 390
ISTASGDGRH YCYPHFTCAV DTENIRRVFN DCRDIIQRMH LRQYELL
Length:397
Mass (Da):46,477
Last modified:April 1, 1993 - v1
Checksum:i9E0C10F42B6C4804
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X63893 mRNA. Translation: CAA45355.1.
PIRiS18963. RGPGA2.
UniGeneiSsc.6675.

Similar proteinsi

Entry informationi

Entry nameiGNAS_PIG
AccessioniPrimary (citable) accession number: P29797
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: August 30, 2017
This is version 122 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families