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Protein

Guanine nucleotide-binding protein G(s) subunit alpha

Gene

GNAS

Organism
Sus scrofa (Pig)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(s) protein is involved in hormonal regulation of adenylate cyclase: it activates the cyclase in response to beta-adrenergic stimuli. Stimulates the Ras signaling pathway via RAPGEF2 (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi54 – 541MagnesiumBy similarity
Metal bindingi189 – 1891MagnesiumBy similarity
Binding sitei369 – 3691GTP; via amide nitrogenBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi47 – 548GTPBy similarity
Nucleotide bindingi183 – 1897GTPBy similarity
Nucleotide bindingi208 – 2125GTPBy similarity
Nucleotide bindingi277 – 2804GTPBy similarity

GO - Molecular functioni

  1. GTPase activity Source: InterPro
  2. GTP binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW
  4. signal transducer activity Source: UniProtKB

GO - Biological processi

  1. adenylate cyclase-activating adrenergic receptor signaling pathway Source: UniProtKB
  2. positive regulation of cAMP biosynthetic process Source: UniProtKB
  3. positive regulation of cAMP-mediated signaling Source: UniProtKB
  4. positive regulation of Ras GTPase activity Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Transducer

Keywords - Ligandi

GTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Guanine nucleotide-binding protein G(s) subunit alpha
Alternative name(s):
Adenylate cyclase-stimulating G alpha protein
Gene namesi
Name:GNAS
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
ProteomesiUP000008227: Unplaced

Subcellular locationi

Cell membrane By similarity; Lipid-anchor By similarity

GO - Cellular componenti

  1. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 397397Guanine nucleotide-binding protein G(s) subunit alphaPRO_0000203724Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-palmitoyl glycineBy similarity
Lipidationi3 – 31S-palmitoyl cysteineBy similarity

Keywords - PTMi

Lipoprotein, Palmitate

Proteomic databases

PaxDbiP29797.

Interactioni

Subunit structurei

G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site.

Structurei

3D structure databases

ProteinModelPortaliP29797.
SMRiP29797. Positions 35-394.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-alpha family. G(s) subfamily.Curated

Phylogenomic databases

eggNOGiNOG325092.
HOGENOMiHOG000038729.
HOVERGENiHBG063184.
InParanoidiP29797.

Family and domain databases

Gene3Di1.10.400.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR000367. Gprotein_alpha_S.
IPR001019. Gprotein_alpha_su.
IPR011025. GproteinA_insert.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR10218. PTHR10218. 1 hit.
PfamiPF00503. G-alpha. 1 hit.
[Graphical view]
PRINTSiPR00318. GPROTEINA.
PR00443. GPROTEINAS.
SMARTiSM00275. G_alpha. 1 hit.
[Graphical view]
SUPFAMiSSF47895. SSF47895. 1 hit.
SSF52540. SSF52540. 2 hits.

Sequencei

Sequence statusi: Complete.

P29797-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGCLGNSKTE DQRNEEKAQR EANKKIEKQL QKDKQVYRAT HRLLLLGAGE
60 70 80 90 100
SGKSTIVKQM RILHVNGFNG DEKATKVQDI KNNLKEAIET IVAAMSNLVP
110 120 130 140 150
PVELANPENQ FRVDYILSVM NVPDFDFPPE FYEHAKALWE DEGVRACYER
160 170 180 190 200
SNEYQLIDCA QYFLDKIDVI KQDDYVPSDQ DLLRCRVLTS GIFETKFQVD
210 220 230 240 250
KVNFHMFDVG GQRDERRKWI QCFNDVTAII FVVASSSYNM VIREDNQTNR
260 270 280 290 300
LQEALNLFKS IWNNRWLRTI SVILFLNKQD LLAEKVLAGK SKIELFVLDD
310 320 330 340 350
RLFQERPFSF IEDYFPEFAR YTTPEDATPE PGEDPRVTRA KYFIRDEFLR
360 370 380 390
ISTASGDGRH YCYPHFTCAV DTENIRRVFN DCRDIIQRMH LRQYELL
Length:397
Mass (Da):46,477
Last modified:April 1, 1993 - v1
Checksum:i9E0C10F42B6C4804
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X63893 mRNA. Translation: CAA45355.1.
PIRiS18963. RGPGA2.
UniGeneiSsc.6675.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X63893 mRNA. Translation: CAA45355.1.
PIRiS18963. RGPGA2.
UniGeneiSsc.6675.

3D structure databases

ProteinModelPortaliP29797.
SMRiP29797. Positions 35-394.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PaxDbiP29797.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiNOG325092.
HOGENOMiHOG000038729.
HOVERGENiHBG063184.
InParanoidiP29797.

Family and domain databases

Gene3Di1.10.400.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR000367. Gprotein_alpha_S.
IPR001019. Gprotein_alpha_su.
IPR011025. GproteinA_insert.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR10218. PTHR10218. 1 hit.
PfamiPF00503. G-alpha. 1 hit.
[Graphical view]
PRINTSiPR00318. GPROTEINA.
PR00443. GPROTEINAS.
SMARTiSM00275. G_alpha. 1 hit.
[Graphical view]
SUPFAMiSSF47895. SSF47895. 1 hit.
SSF52540. SSF52540. 2 hits.
ProtoNetiSearch...

Publicationsi

  1. "Isolation and sequence of a porcine cDNA for the alpha-S subunit of the cardiac stimulatory GTP-binding protein (Gs)."
    Roth D.A., Kay R.A.M., Hammond H.K.
    Submitted (JAN-1992) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Heart.

Entry informationi

Entry nameiGNAS_PIG
AccessioniPrimary (citable) accession number: P29797
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: January 7, 2015
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.