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P29797

- GNAS_PIG

UniProt

P29797 - GNAS_PIG

Protein

Guanine nucleotide-binding protein G(s) subunit alpha

Gene

GNAS

Organism
Sus scrofa (Pig)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 105 (01 Oct 2014)
      Sequence version 1 (01 Apr 1993)
      Previous versions | rss
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    Functioni

    Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(s) protein is involved in hormonal regulation of adenylate cyclase: it activates the cyclase in response to beta-adrenergic stimuli. Stimulates the Ras signaling pathway via RAPGEF2 By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi54 – 541MagnesiumBy similarity
    Metal bindingi189 – 1891MagnesiumBy similarity
    Binding sitei369 – 3691GTP; via amide nitrogenBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi47 – 548GTPBy similarity
    Nucleotide bindingi183 – 1897GTPBy similarity
    Nucleotide bindingi208 – 2125GTPBy similarity
    Nucleotide bindingi277 – 2804GTPBy similarity

    GO - Molecular functioni

    1. GTPase activity Source: InterPro
    2. GTP binding Source: UniProtKB-KW
    3. metal ion binding Source: UniProtKB-KW
    4. signal transducer activity Source: UniProtKB

    GO - Biological processi

    1. adenylate cyclase-activating adrenergic receptor signaling pathway Source: UniProtKB
    2. positive regulation of cAMP biosynthetic process Source: UniProtKB
    3. positive regulation of cAMP-mediated signaling Source: UniProtKB
    4. positive regulation of Ras GTPase activity Source: UniProtKB

    Keywords - Molecular functioni

    Transducer

    Keywords - Ligandi

    GTP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Guanine nucleotide-binding protein G(s) subunit alpha
    Alternative name(s):
    Adenylate cyclase-stimulating G alpha protein
    Gene namesi
    Name:GNAS
    OrganismiSus scrofa (Pig)
    Taxonomic identifieri9823 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
    ProteomesiUP000008227: Unplaced

    Subcellular locationi

    Cell membrane By similarity; Lipid-anchor By similarity

    GO - Cellular componenti

    1. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 397397Guanine nucleotide-binding protein G(s) subunit alphaPRO_0000203724Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Lipidationi2 – 21N-palmitoyl glycineBy similarity
    Lipidationi3 – 31S-palmitoyl cysteineBy similarity

    Keywords - PTMi

    Lipoprotein, Palmitate

    Proteomic databases

    PaxDbiP29797.

    Interactioni

    Subunit structurei

    G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site.

    Structurei

    3D structure databases

    ProteinModelPortaliP29797.
    SMRiP29797. Positions 35-394.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the G-alpha family. G(s) subfamily.Curated

    Phylogenomic databases

    eggNOGiNOG325092.
    HOGENOMiHOG000038729.
    HOVERGENiHBG063184.

    Family and domain databases

    Gene3Di1.10.400.10. 1 hit.
    3.40.50.300. 2 hits.
    InterProiIPR000367. Gprotein_alpha_S.
    IPR001019. Gprotein_alpha_su.
    IPR011025. GproteinA_insert.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PANTHERiPTHR10218. PTHR10218. 1 hit.
    PfamiPF00503. G-alpha. 1 hit.
    [Graphical view]
    PRINTSiPR00318. GPROTEINA.
    PR00443. GPROTEINAS.
    SMARTiSM00275. G_alpha. 1 hit.
    [Graphical view]
    SUPFAMiSSF47895. SSF47895. 1 hit.
    SSF52540. SSF52540. 2 hits.

    Sequencei

    Sequence statusi: Complete.

    P29797-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGCLGNSKTE DQRNEEKAQR EANKKIEKQL QKDKQVYRAT HRLLLLGAGE    50
    SGKSTIVKQM RILHVNGFNG DEKATKVQDI KNNLKEAIET IVAAMSNLVP 100
    PVELANPENQ FRVDYILSVM NVPDFDFPPE FYEHAKALWE DEGVRACYER 150
    SNEYQLIDCA QYFLDKIDVI KQDDYVPSDQ DLLRCRVLTS GIFETKFQVD 200
    KVNFHMFDVG GQRDERRKWI QCFNDVTAII FVVASSSYNM VIREDNQTNR 250
    LQEALNLFKS IWNNRWLRTI SVILFLNKQD LLAEKVLAGK SKIELFVLDD 300
    RLFQERPFSF IEDYFPEFAR YTTPEDATPE PGEDPRVTRA KYFIRDEFLR 350
    ISTASGDGRH YCYPHFTCAV DTENIRRVFN DCRDIIQRMH LRQYELL 397
    Length:397
    Mass (Da):46,477
    Last modified:April 1, 1993 - v1
    Checksum:i9E0C10F42B6C4804
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X63893 mRNA. Translation: CAA45355.1.
    PIRiS18963. RGPGA2.
    UniGeneiSsc.6675.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X63893 mRNA. Translation: CAA45355.1 .
    PIRi S18963. RGPGA2.
    UniGenei Ssc.6675.

    3D structure databases

    ProteinModelPortali P29797.
    SMRi P29797. Positions 35-394.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PaxDbi P29797.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Phylogenomic databases

    eggNOGi NOG325092.
    HOGENOMi HOG000038729.
    HOVERGENi HBG063184.

    Family and domain databases

    Gene3Di 1.10.400.10. 1 hit.
    3.40.50.300. 2 hits.
    InterProi IPR000367. Gprotein_alpha_S.
    IPR001019. Gprotein_alpha_su.
    IPR011025. GproteinA_insert.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    PANTHERi PTHR10218. PTHR10218. 1 hit.
    Pfami PF00503. G-alpha. 1 hit.
    [Graphical view ]
    PRINTSi PR00318. GPROTEINA.
    PR00443. GPROTEINAS.
    SMARTi SM00275. G_alpha. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47895. SSF47895. 1 hit.
    SSF52540. SSF52540. 2 hits.
    ProtoNeti Search...

    Publicationsi

    1. "Isolation and sequence of a porcine cDNA for the alpha-S subunit of the cardiac stimulatory GTP-binding protein (Gs)."
      Roth D.A., Kay R.A.M., Hammond H.K.
      Submitted (JAN-1992) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Heart.

    Entry informationi

    Entry nameiGNAS_PIG
    AccessioniPrimary (citable) accession number: P29797
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1993
    Last sequence update: April 1, 1993
    Last modified: October 1, 2014
    This is version 105 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3