P29797 (GNAS_PIG) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Guanine nucleotide-binding protein G(s) subunit alpha

Alternative name(s):
Adenylate cyclase-stimulating G alpha protein

Gene names

Name:

GNAS

Organism

Sus scrofa (Pig) [Reference proteome]

Taxonomic identifier

9823 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Cetartiodactyla › Suina › Suidae › Sus

Protein attributes

Sequence length	397 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(s) protein is involved in hormonal regulation of adenylate cyclase: it activates the cyclase in response to beta-adrenergic stimuli.
Subunit structure	G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site.
Subcellular location	Cell membrane; Lipid-anchor By similarity.
Sequence similarities	Belongs to the G-alpha family. G(s) subfamily.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Ligand	GTP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Transducer
PTM	Lipoprotein Palmitate Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	G-protein coupled receptor signaling pathway Inferred from electronic annotation. Source: InterPro
Cellular_component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW signal transducer activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 397	396	Guanine nucleotide-binding protein G(s) subunit alpha		PRO_0000203724
Regions
	Nucleotide binding	47 – 54	8	GTP By similarity
	Nucleotide binding	183 – 189	7	GTP By similarity
	Nucleotide binding	208 – 212	5	GTP By similarity
	Nucleotide binding	277 – 280	4	GTP By similarity
Sites
	Metal binding	54	1	Magnesium By similarity
	Metal binding	189	1	Magnesium By similarity
	Binding site	369	1	GTP; via amide nitrogen By similarity
Amino acid modifications
	Modified residue	51	1	Phosphoserine By similarity
	Lipidation	2	1	N-palmitoyl glycine By similarity
	Lipidation	3	1	S-palmitoyl cysteine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P29797 [UniParc]. Last modified April 1, 1993. Version 1. Checksum: 9E0C10F42B6C4804	FASTA	397	46,477
	10 20 30 40 50 60 MGCLGNSKTE DQRNEEKAQR EANKKIEKQL QKDKQVYRAT HRLLLLGAGE SGKSTIVKQM 70 80 90 100 110 120 RILHVNGFNG DEKATKVQDI KNNLKEAIET IVAAMSNLVP PVELANPENQ FRVDYILSVM 130 140 150 160 170 180 NVPDFDFPPE FYEHAKALWE DEGVRACYER SNEYQLIDCA QYFLDKIDVI KQDDYVPSDQ 190 200 210 220 230 240 DLLRCRVLTS GIFETKFQVD KVNFHMFDVG GQRDERRKWI QCFNDVTAII FVVASSSYNM 250 260 270 280 290 300 VIREDNQTNR LQEALNLFKS IWNNRWLRTI SVILFLNKQD LLAEKVLAGK SKIELFVLDD 310 320 330 340 350 360 RLFQERPFSF IEDYFPEFAR YTTPEDATPE PGEDPRVTRA KYFIRDEFLR ISTASGDGRH 370 380 390 YCYPHFTCAV DTENIRRVFN DCRDIIQRMH LRQYELL

References

[1]	"Isolation and sequence of a porcine cDNA for the alpha-S subunit of the cardiac stimulatory GTP-binding protein (Gs)." Roth D.A., Kay R.A.M., Hammond H.K. Submitted (JAN-1992) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Heart.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X63893 mRNA. Translation: CAA45355.1.
PIR	RGPGA2. S18963.
UniGene	Ssc.6675.
3D structure databases
ProteinModelPortal	P29797.
SMR	P29797. Positions 35-394.
ModBase	Search...
Proteomic databases
PaxDb	P29797.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
eggNOG	NOG325092.
HOGENOM	HOG000038729.
HOVERGEN	HBG063184.
Family and domain databases
Gene3D	1.10.400.10. 1 hit.
InterPro	IPR000367. Gprotein_alpha_S. IPR001019. Gprotein_alpha_su. IPR011025. GproteinA_insert. [Graphical view]
PANTHER	PTHR10218. PTHR10218. 1 hit.
Pfam	PF00503. G-alpha. 1 hit. [Graphical view]
PRINTS	PR00318. GPROTEINA. PR00443. GPROTEINAS.
SMART	SM00275. G_alpha. 1 hit. [Graphical view]
SUPFAM	SSF47895. Transducn_insert. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GNAS_PIG

Accession

Primary (citable) accession number: P29797

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	May 1, 2013
This is version 99 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program