Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Fusion glycoprotein F0

Gene

F

Organism
Bovine respiratory syncytial virus (strain A51908) (BRS)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

During virus entry, induces fusion of viral and cellular membranes leading to delivery of the nucleocapsid into the cytoplasm. The fusogenic activity is inactive untill entry into host cell endosome, where a furin-like protease cleaves off a small peptide between F1 and F2. Interacts directly with heparan sulfate and may participate in virus attachment. Later in infection, proteins F expressed at the plasma membrane of infected cells can mediate fusion with adjacent cells to form syncytia, a cytopathic effect that could lead to tissue necrosis. The fusion protein is also able to trigger p53-dependent apoptosis.By similarity

GO - Biological processi

Keywordsi

Biological processFusion of virus membrane with host cell membrane, Fusion of virus membrane with host membrane, Syncytium formation induced by viral infection, Viral penetration into host cytoplasm, Virus entry into host cell

Names & Taxonomyi

Protein namesi
Recommended name:
Fusion glycoprotein F0
Cleaved into the following 4 chains:
Fusion glycoprotein F2'By similarity
Interchain peptideBy similarity
Gene namesi
Name:F
OrganismiBovine respiratory syncytial virus (strain A51908) (BRS)
Taxonomic identifieri11247 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA negative-strand virusesMononegaviralesPneumoviridaeOrthopneumovirus
Virus hostiBos taurus (Bovine) [TaxID: 9913]
Proteomesi
  • UP000007616 Componenti: Genome

Subcellular locationi

P29791:
  • Virion membrane By similarity; Single-pass type I membrane protein By similarity
  • Host cell membrane By similarity; Single-pass membrane protein By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini26 – 527ExtracellularBy similarityAdd BLAST502
Transmembranei528 – 548HelicalSequence analysisAdd BLAST21
Topological domaini549 – 572CytoplasmicBy similarityAdd BLAST24

GO - Cellular componenti

Keywords - Cellular componenti

Host cell membrane, Host membrane, Membrane, Viral envelope protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 25Sequence analysisAdd BLAST25
ChainiPRO_000003922526 – 572Fusion glycoprotein F0Add BLAST547
ChainiPRO_000003922626 – 136Fusion glycoprotein F2Add BLAST111
ChainiPRO_000043265726 – 109Fusion glycoprotein F2'By similarityAdd BLAST84
PeptideiPRO_0000432658110 – 136Interchain peptideBy similarityAdd BLAST27
ChainiPRO_0000039227137 – 572Fusion glycoprotein F1Add BLAST436

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi27N-linked (GlcNAc...) asparagine; by hostSequence analysis1
Disulfide bondi69 ↔ 212Interchain (between F2 and F1 chains)By similarity
Glycosylationi70N-linked (GlcNAc...) asparagine; by hostSequence analysis1
Glycosylationi120N-linked (GlcNAc...) asparagine; by hostSequence analysis1
Disulfide bondi356 ↔ 365By similarity
Disulfide bondi380 ↔ 391By similarity
Glycosylationi498N-linked (GlcNAc...) asparagine; by hostSequence analysis1
Lipidationi548S-palmitoyl cysteine; by hostBy similarity1

Post-translational modificationi

The F glycoprotein is synthesized as a F0 inactive precursor that is heavily N-glycosylated and processed by host cell furin in the Golgi to yield the mature F1 and F2 proteins. The cleavage site between F1 and F2 requires the minimal sequence [KR]-X-[KR]-R. During entry a furin-like protease cleaves F2 into F2' and a small peptide, leading to the activation of fusogenic function.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei109 – 110Cleavage; by hostBy similarity2
Sitei136 – 137Cleavage; by hostBy similarity2
Sitei136 – 137Cleavage; by host furinBy similarity2

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

Interactioni

Subunit structurei

Homotrimer. Interacts with glycoprotein G. Interacts with host RHOA; this interaction facilitates virus-induced syncytium formation.By similarity

Structurei

3D structure databases

ProteinModelPortaliP29791.
SMRiP29791.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni137 – 157Fusion peptideBy similarityAdd BLAST21

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili155 – 183Sequence analysisAdd BLAST29
Coiled coili489 – 514Sequence analysisAdd BLAST26

Sequence similaritiesi

Keywords - Domaini

Coiled coil, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

OrthoDBiVOG0900006Q.

Family and domain databases

InterProiView protein in InterPro
IPR000776. Fusion_F0_Paramyxovir.
IPR013055. Tachy_Neuro_lke_CS.
PfamiView protein in Pfam
PF00523. Fusion_gly. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P29791-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATTTMRMII SIILISTYVP HITLCQNITE EFYQSTCSAV SRGYLSALRT
60 70 80 90 100
GWYTSVVTIE LSKIQKNVCN GTDSKVKLIK QELERYNNAV AELQSLMQNE
110 120 130 140 150
PTSSSRAKRG IPESIHYTRN STKKFYGLMG KKRKRRFLGF LLGIGSAIAS
160 170 180 190 200
GVAVSKVLHL EGEVNKIKNA LLSTNKAVVS LSNGVSVLTS KVLDLKNYID
210 220 230 240 250
KELLPKVNNH DCRISNIATV IEFQQKNNRL LEIAREFSVN AGITTPLSTY
260 270 280 290 300
MLTNSELLSI INDMPITNDQ KKLMSVCQIV RQQSYSIMSV LREVIAYVVQ
310 320 330 340 350
LPLYGVIDTP CWKLHTSPLC TTDNKEGSNI CLTRTDRGWY CDNAGSVSFF
360 370 380 390 400
PQAETCKVQS NRVFCDTMNS LTLPTDVNLC NTDIFNSKYD CKIMTSKTDI
410 420 430 440 450
SSSVITSIGA IVSCYGKTKC TASNKNRGII KTFSNGCDYV SNKGVDTVSV
460 470 480 490 500
GNTLYYVNKL EGKALYIKGE PIINYYNPLV FPSDEFDASI AQVNAKINQS
510 520 530 540 550
LAFIRRSDEL LHSVDVGKST TNVVITTIII VIVVVILMLI TVGLLFYCKT
560 570
RSTPIMLGKD QLSSINNLSF SK
Length:572
Mass (Da):63,443
Last modified:April 1, 1993 - v1
Checksum:iA055E5E65801663E
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti5T → A in strain: ATCC 51908. 1
Natural varianti14L → F in strain: ATCC 51908. 1
Natural varianti20P → T in strain: ATCC 51908. 1
Natural varianti71G → S in strain: ATCC 51908. 1
Natural varianti91A → V. 1
Natural varianti102 – 104TSS → ASF in strain: ATCC 51908. 3
Natural varianti114S → L in strain: ATCC 51908. 1
Natural varianti216 – 218NIA → KIE in strain: ATCC 51908. 3
Natural varianti260I → L in strain: ATCC 51908. 1
Natural varianti276 – 277VC → SNV. 2
Natural varianti291 – 292LR → VKE. 2
Natural varianti303L → I in strain: ATCC 51908. 1
Natural varianti325K → E. 1
Natural varianti353A → T in strain: ATCC 51908. 1
Natural varianti387S → A. 1
Natural varianti387S → T in strain: ATCC 51908. 1
Natural varianti477N → D in strain: ATCC 51908. 1
Natural varianti541T → A in strain: ATCC 51908. 1
Natural varianti551R → K in strain: ATCC 51908. 1
Natural varianti564S → G in strain: ATCC 51908. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M82816 mRNA. Translation: AAA42804.1.
AF295543 Genomic RNA. Translation: AAL49399.1.
AF295544 Genomic RNA. Translation: AAL49410.1.
PIRiJQ1481. VGNZBA.

Similar proteinsi

Entry informationi

Entry nameiFUS_BRSVA
AccessioniPrimary (citable) accession number: P29791
Secondary accession number(s): Q8V688, Q8V691
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: September 27, 2017
This is version 91 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families