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P29787 (TRY5_AEDAE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Trypsin 5G1

EC=3.4.21.4
Gene names
ORF Names:AAEL013712
OrganismAedes aegypti (Yellowfever mosquito) (Culex aegypti) [Reference proteome]
Taxonomic identifier7159 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraNematoceraCulicoideaCulicidaeCulicinaeAediniAedesStegomyia

Protein attributes

Sequence length266 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Major function may be to aid in digestion of the blood meal.

Catalytic activity

Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

Subcellular location

Secretedextracellular space.

Tissue specificity

Midgut.

Sequence similarities

Belongs to the peptidase S1 family.

Contains 1 peptidase S1 domain.

Sequence caution

The sequence CAA45715.1 differs from that shown. Reason: Frameshift at position 35.

Ontologies

Keywords
   Biological processDigestion
   Cellular componentSecreted
   DomainSignal
   Molecular functionHydrolase
Protease
Serine protease
   PTMDisulfide bond
Zymogen
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processdigestion

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentextracellular space

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionserine-type endopeptidase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1818 Potential
Propeptide19 – 3921Activation peptide
PRO_0000028241
Chain40 – 266227Trypsin 5G1
PRO_0000028242

Regions

Domain40 – 265226Peptidase S1

Sites

Active site811Charge relay system By similarity
Active site1251Charge relay system By similarity
Active site2211Charge relay system By similarity
Site2151Required for specificity By similarity

Amino acid modifications

Disulfide bond66 ↔ 82 By similarity
Disulfide bond190 ↔ 206 By similarity
Disulfide bond217 ↔ 241 By similarity

Experimental info

Sequence conflict1511A → T in CAA45715. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P29787 [UniParc].

Last modified September 2, 2008. Version 2.
Checksum: 63CB0B37F1B022C6

FASTA26628,246
        10         20         30         40         50         60 
MTRIILILTA TFFACALGAS TGGSHPLRPW WNALRSSGRI VGGFEVPVEE VPFQVSLSGV 

        70         80         90        100        110        120 
GSSHFCGGSL LSERWVMTAG HCAASGQTNL QVRIGSSQHA SGGQLIKVKK VNRHPKYDEV 

       130        140        150        160        170        180 
TTDYDFALLE LEETVTFSDS CAPVKLPQKD APVNEGTCLQ VSGWGNTQNP SESSEVLRAA 

       190        200        210        220        230        240 
YVPAVSQKEC HKAYLSFGGV TDRMVCAGFK EGGKDSCQGD SGGPLVHDNT LVGVVSWGYG 

       250        260 
CAQAGYPGVY ARVASVRDWV KEVSGL 

« Hide

References

« Hide 'large scale' references
[1]"Genome sequence of Aedes aegypti, a major arbovirus vector."
Nene V., Wortman J.R., Lawson D., Haas B.J., Kodira C.D., Tu Z.J., Loftus B.J., Xi Z., Megy K., Grabherr M., Ren Q., Zdobnov E.M., Lobo N.F., Campbell K.S., Brown S.E., Bonaldo M.F., Zhu J., Sinkins S.P. expand/collapse author list , Hogenkamp D.G., Amedeo P., Arensburger P., Atkinson P.W., Bidwell S.L., Biedler J., Birney E., Bruggner R.V., Costas J., Coy M.R., Crabtree J., Crawford M., DeBruyn B., DeCaprio D., Eiglmeier K., Eisenstadt E., El-Dorry H., Gelbart W.M., Gomes S.L., Hammond M., Hannick L.I., Hogan J.R., Holmes M.H., Jaffe D., Johnston S.J., Kennedy R.C., Koo H., Kravitz S., Kriventseva E.V., Kulp D., Labutti K., Lee E., Li S., Lovin D.D., Mao C., Mauceli E., Menck C.F., Miller J.R., Montgomery P., Mori A., Nascimento A.L., Naveira H.F., Nusbaum C., O'Leary S.B., Orvis J., Pertea M., Quesneville H., Reidenbach K.R., Rogers Y.-H.C., Roth C.W., Schneider J.R., Schatz M., Shumway M., Stanke M., Stinson E.O., Tubio J.M.C., Vanzee J.P., Verjovski-Almeida S., Werner D., White O.R., Wyder S., Zeng Q., Zhao Q., Zhao Y., Hill C.A., Raikhel A.S., Soares M.B., Knudson D.L., Lee N.H., Galagan J., Salzberg S.L., Paulsen I.T., Dimopoulos G., Collins F.H., Bruce B., Fraser-Liggett C.M., Severson D.W.
Science 316:1718-1723(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: LVPib12.
[2]"Isolation, sequencing and characterization of two cDNA clones coding for trypsin-like enzymes from the midgut of Aedes aegypti."
Kalhok S., Tabak L.M., Prosser D.E., Brook W., Downer A.E.R., White B.N.
Insect Mol. Biol. 2:71-79(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 29-266.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CH478086 Genomic DNA. Translation: EAT34029.1.
X64363 mRNA. Translation: CAA45715.1. Frameshift.
PIRTRWV5Y. S19891.
RefSeqXP_001663895.1. XM_001663845.1.
UniGeneAae.25940.

3D structure databases

ProteinModelPortalP29787.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPSS01.130.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaAAEL013712-RA; AAEL013712-PA; AAEL013712.
GeneID5578510.
KEGGaag:AaeL_AAEL013712.
VectorBaseAAEL013712. Aedes aegypti.

Phylogenomic databases

eggNOGCOG5640.
HOGENOMHOG000251820.
InParanoidP29787.
KOK01312.
OrthoDBEOG75B84T.
PhylomeDBP29787.

Family and domain databases

InterProIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSPR00722. CHYMOTRYPSIN.
SMARTSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMSSF50494. SSF50494. 1 hit.
PROSITEPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRY5_AEDAE
AccessionPrimary (citable) accession number: P29787
Secondary accession number(s): Q16ID7
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: September 2, 2008
Last modified: May 14, 2014
This is version 96 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries