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P29787

- TRY5_AEDAE

UniProt

P29787 - TRY5_AEDAE

Protein

Trypsin 5G1

Gene

AAEL013712

Organism
Aedes aegypti (Yellowfever mosquito) (Culex aegypti)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 97 (01 Oct 2014)
      Sequence version 2 (02 Sep 2008)
      Previous versions | rss
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    Functioni

    Major function may be to aid in digestion of the blood meal.

    Catalytic activityi

    Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei81 – 811Charge relay systemBy similarity
    Active sitei125 – 1251Charge relay systemBy similarity
    Sitei215 – 2151Required for specificityBy similarity
    Active sitei221 – 2211Charge relay systemBy similarity

    GO - Molecular functioni

    1. serine-type endopeptidase activity Source: InterPro

    GO - Biological processi

    1. digestion Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase, Protease, Serine protease

    Keywords - Biological processi

    Digestion

    Protein family/group databases

    MEROPSiS01.130.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Trypsin 5G1 (EC:3.4.21.4)
    Gene namesi
    ORF Names:AAEL013712
    OrganismiAedes aegypti (Yellowfever mosquito) (Culex aegypti)
    Taxonomic identifieri7159 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraNematoceraCulicoideaCulicidaeCulicinaeAediniAedesStegomyia
    ProteomesiUP000008820: Unassembled WGS sequence

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular space Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1818Sequence AnalysisAdd
    BLAST
    Propeptidei19 – 3921Activation peptidePRO_0000028241Add
    BLAST
    Chaini40 – 266227Trypsin 5G1PRO_0000028242Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi66 ↔ 82PROSITE-ProRule annotation
    Disulfide bondi190 ↔ 206PROSITE-ProRule annotation
    Disulfide bondi217 ↔ 241PROSITE-ProRule annotation

    Keywords - PTMi

    Disulfide bond, Zymogen

    Expressioni

    Tissue specificityi

    Midgut.

    Structurei

    3D structure databases

    ProteinModelPortaliP29787.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini40 – 265226Peptidase S1PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase S1 family.PROSITE-ProRule annotation
    Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG5640.
    HOGENOMiHOG000251820.
    InParanoidiP29787.
    KOiK01312.
    OrthoDBiEOG75B84T.
    PhylomeDBiP29787.

    Family and domain databases

    InterProiIPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view]
    PfamiPF00089. Trypsin. 1 hit.
    [Graphical view]
    PRINTSiPR00722. CHYMOTRYPSIN.
    SMARTiSM00020. Tryp_SPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF50494. SSF50494. 1 hit.
    PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P29787-1 [UniParc]FASTAAdd to Basket

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    MTRIILILTA TFFACALGAS TGGSHPLRPW WNALRSSGRI VGGFEVPVEE    50
    VPFQVSLSGV GSSHFCGGSL LSERWVMTAG HCAASGQTNL QVRIGSSQHA 100
    SGGQLIKVKK VNRHPKYDEV TTDYDFALLE LEETVTFSDS CAPVKLPQKD 150
    APVNEGTCLQ VSGWGNTQNP SESSEVLRAA YVPAVSQKEC HKAYLSFGGV 200
    TDRMVCAGFK EGGKDSCQGD SGGPLVHDNT LVGVVSWGYG CAQAGYPGVY 250
    ARVASVRDWV KEVSGL 266
    Length:266
    Mass (Da):28,246
    Last modified:September 2, 2008 - v2
    Checksum:i63CB0B37F1B022C6
    GO

    Sequence cautioni

    The sequence CAA45715.1 differs from that shown. Reason: Frameshift at position 35.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti151 – 1511A → T in CAA45715. (PubMed:9087545)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CH478086 Genomic DNA. Translation: EAT34029.1.
    X64363 mRNA. Translation: CAA45715.1. Frameshift.
    PIRiS19891. TRWV5Y.
    RefSeqiXP_001663895.1. XM_001663845.1.
    UniGeneiAae.25940.

    Genome annotation databases

    EnsemblMetazoaiAAEL013712-RA; AAEL013712-PA; AAEL013712.
    GeneIDi5578510.
    KEGGiaag:AaeL_AAEL013712.
    VectorBaseiAAEL013712. Aedes aegypti.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CH478086 Genomic DNA. Translation: EAT34029.1 .
    X64363 mRNA. Translation: CAA45715.1 . Frameshift.
    PIRi S19891. TRWV5Y.
    RefSeqi XP_001663895.1. XM_001663845.1.
    UniGenei Aae.25940.

    3D structure databases

    ProteinModelPortali P29787.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi S01.130.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai AAEL013712-RA ; AAEL013712-PA ; AAEL013712 .
    GeneIDi 5578510.
    KEGGi aag:AaeL_AAEL013712.
    VectorBasei AAEL013712. Aedes aegypti.

    Phylogenomic databases

    eggNOGi COG5640.
    HOGENOMi HOG000251820.
    InParanoidi P29787.
    KOi K01312.
    OrthoDBi EOG75B84T.
    PhylomeDBi P29787.

    Family and domain databases

    InterProi IPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view ]
    Pfami PF00089. Trypsin. 1 hit.
    [Graphical view ]
    PRINTSi PR00722. CHYMOTRYPSIN.
    SMARTi SM00020. Tryp_SPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50494. SSF50494. 1 hit.
    PROSITEi PS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of Aedes aegypti, a major arbovirus vector."
      Nene V., Wortman J.R., Lawson D., Haas B.J., Kodira C.D., Tu Z.J., Loftus B.J., Xi Z., Megy K., Grabherr M., Ren Q., Zdobnov E.M., Lobo N.F., Campbell K.S., Brown S.E., Bonaldo M.F., Zhu J., Sinkins S.P.
      , Hogenkamp D.G., Amedeo P., Arensburger P., Atkinson P.W., Bidwell S.L., Biedler J., Birney E., Bruggner R.V., Costas J., Coy M.R., Crabtree J., Crawford M., DeBruyn B., DeCaprio D., Eiglmeier K., Eisenstadt E., El-Dorry H., Gelbart W.M., Gomes S.L., Hammond M., Hannick L.I., Hogan J.R., Holmes M.H., Jaffe D., Johnston S.J., Kennedy R.C., Koo H., Kravitz S., Kriventseva E.V., Kulp D., Labutti K., Lee E., Li S., Lovin D.D., Mao C., Mauceli E., Menck C.F., Miller J.R., Montgomery P., Mori A., Nascimento A.L., Naveira H.F., Nusbaum C., O'Leary S.B., Orvis J., Pertea M., Quesneville H., Reidenbach K.R., Rogers Y.-H.C., Roth C.W., Schneider J.R., Schatz M., Shumway M., Stanke M., Stinson E.O., Tubio J.M.C., Vanzee J.P., Verjovski-Almeida S., Werner D., White O.R., Wyder S., Zeng Q., Zhao Q., Zhao Y., Hill C.A., Raikhel A.S., Soares M.B., Knudson D.L., Lee N.H., Galagan J., Salzberg S.L., Paulsen I.T., Dimopoulos G., Collins F.H., Bruce B., Fraser-Liggett C.M., Severson D.W.
      Science 316:1718-1723(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: LVPib12.
    2. "Isolation, sequencing and characterization of two cDNA clones coding for trypsin-like enzymes from the midgut of Aedes aegypti."
      Kalhok S., Tabak L.M., Prosser D.E., Brook W., Downer A.E.R., White B.N.
      Insect Mol. Biol. 2:71-79(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 29-266.

    Entry informationi

    Entry nameiTRY5_AEDAE
    AccessioniPrimary (citable) accession number: P29787
    Secondary accession number(s): Q16ID7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1993
    Last sequence update: September 2, 2008
    Last modified: October 1, 2014
    This is version 97 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3