P29787 (TRY5_AEDAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Trypsin 5G1
EC=3.4.21.4

Gene names

ORF Names:

AAEL013712

Organism

Aedes aegypti (Yellowfever mosquito) (Culex aegypti) [Reference proteome]

Taxonomic identifier

7159 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Nematocera › Culicimorpha › Culicoidea › Culicidae › Culicinae › Aedini › Aedes › Stegomyia

Protein attributes

Sequence length	266 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Major function may be to aid in digestion of the blood meal.
Catalytic activity	Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.
Subcellular location	Secreted › extracellular space.
Tissue specificity	Midgut.
Sequence similarities	Belongs to the peptidase S1 family. Contains 1 peptidase S1 domain.
Sequence caution	The sequence CAA45715.1 differs from that shown. Reason: Frameshift at position 35.

Ontologies

Keywords
Biological process	Digestion
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Zymogen
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	digestion Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	Potential
	Propeptide	19 – 39	21	Activation peptide		PRO_0000028241
	Chain	40 – 266	227	Trypsin 5G1		PRO_0000028242
Regions
	Domain	40 – 265	226	Peptidase S1
Sites
	Active site	81	1	Charge relay system By similarity
	Active site	125	1	Charge relay system By similarity
	Active site	221	1	Charge relay system By similarity
	Site	215	1	Required for specificity By similarity
Amino acid modifications
	Disulfide bond	66 ↔ 82		By similarity
	Disulfide bond	190 ↔ 206		By similarity
	Disulfide bond	217 ↔ 241		By similarity
Experimental info
	Sequence conflict	151	1	A → T in CAA45715. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P29787 [UniParc]. Last modified September 2, 2008. Version 2. Checksum: 63CB0B37F1B022C6	FASTA	266	28,246
	10 20 30 40 50 60 MTRIILILTA TFFACALGAS TGGSHPLRPW WNALRSSGRI VGGFEVPVEE VPFQVSLSGV 70 80 90 100 110 120 GSSHFCGGSL LSERWVMTAG HCAASGQTNL QVRIGSSQHA SGGQLIKVKK VNRHPKYDEV 130 140 150 160 170 180 TTDYDFALLE LEETVTFSDS CAPVKLPQKD APVNEGTCLQ VSGWGNTQNP SESSEVLRAA 190 200 210 220 230 240 YVPAVSQKEC HKAYLSFGGV TDRMVCAGFK EGGKDSCQGD SGGPLVHDNT LVGVVSWGYG 250 260 CAQAGYPGVY ARVASVRDWV KEVSGL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genome sequence of Aedes aegypti, a major arbovirus vector." Nene V., Wortman J.R., Lawson D., Haas B.J., Kodira C.D., Tu Z.J., Loftus B.J., Xi Z., Megy K., Grabherr M., Ren Q., Zdobnov E.M., Lobo N.F., Campbell K.S., Brown S.E., Bonaldo M.F., Zhu J., Sinkins S.P. , Hogenkamp D.G., Amedeo P., Arensburger P., Atkinson P.W., Bidwell S.L., Biedler J., Birney E., Bruggner R.V., Costas J., Coy M.R., Crabtree J., Crawford M., DeBruyn B., DeCaprio D., Eiglmeier K., Eisenstadt E., El-Dorry H., Gelbart W.M., Gomes S.L., Hammond M., Hannick L.I., Hogan J.R., Holmes M.H., Jaffe D., Johnston S.J., Kennedy R.C., Koo H., Kravitz S., Kriventseva E.V., Kulp D., Labutti K., Lee E., Li S., Lovin D.D., Mao C., Mauceli E., Menck C.F., Miller J.R., Montgomery P., Mori A., Nascimento A.L., Naveira H.F., Nusbaum C., O'Leary S.B., Orvis J., Pertea M., Quesneville H., Reidenbach K.R., Rogers Y.-H.C., Roth C.W., Schneider J.R., Schatz M., Shumway M., Stanke M., Stinson E.O., Tubio J.M.C., Vanzee J.P., Verjovski-Almeida S., Werner D., White O.R., Wyder S., Zeng Q., Zhao Q., Zhao Y., Hill C.A., Raikhel A.S., Soares M.B., Knudson D.L., Lee N.H., Galagan J., Salzberg S.L., Paulsen I.T., Dimopoulos G., Collins F.H., Bruce B., Fraser-Liggett C.M., Severson D.W. Science 316:1718-1723(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LVPib12.
[2]	"Isolation, sequencing and characterization of two cDNA clones coding for trypsin-like enzymes from the midgut of Aedes aegypti." Kalhok S., Tabak L.M., Prosser D.E., Brook W., Downer A.E.R., White B.N. Insect Mol. Biol. 2:71-79(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 29-266.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CH478086 Genomic DNA. Translation: EAT34029.1. X64363 mRNA. Translation: CAA45715.1. Frameshift.
PIR	TRWV5Y. S19891.
RefSeq	XP_001663895.1. XM_001663845.1.
UniGene	Aae.25940.
3D structure databases
ProteinModelPortal	P29787.
ModBase	Search...
Protein family/group databases
MEROPS	S01.130.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	AAEL013712-RA; AAEL013712-PA; AAEL013712.
GeneID	5578510.
KEGG	aag:AaeL_AAEL013712.
VectorBase	AAEL013712. Aedes aegypti.
Phylogenomic databases
eggNOG	COG5640.
HOGENOM	HOG000251820.
InParanoid	P29787.
KO	K01312.
OMA	MITENMI.
OrthoDB	EOG4VDNFK.
PhylomeDB	P29787.
Family and domain databases
InterPro	IPR001254. Peptidase_S1. IPR018114. Peptidase_S1_AS. IPR001314. Peptidase_S1A. IPR009003. Trypsin-like_Pept_dom. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
SUPFAM	SSF50494. Pept_Ser_Cys. 1 hit.
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRY5_AEDAE

Accession

Primary (citable) accession number: P29787
Secondary accession number(s): Q16ID7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	September 2, 2008
Last modified:	April 3, 2013
This is version 93 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)