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P29786 (TRY3_AEDAE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Trypsin 3A1

EC=3.4.21.4
Gene names
ORF Names:AAEL007818
OrganismAedes aegypti (Yellowfever mosquito) (Culex aegypti) [Reference proteome]
Taxonomic identifier7159 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraNematoceraCulicoideaCulicidaeCulicinaeAediniAedesStegomyia

Protein attributes

Sequence length254 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Major function may be to aid in digestion of the blood meal. Ref.3

Catalytic activity

Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

Subcellular location

Secretedextracellular space Ref.3.

Tissue specificity

Midgut. Ref.3

Induction

By blood feed. Ref.3

Sequence similarities

Belongs to the peptidase S1 family.

Contains 1 peptidase S1 domain.

Ontologies

Keywords
   Biological processDigestion
   Cellular componentSecreted
   Coding sequence diversityAlternative splicing
   DomainSignal
   Molecular functionHydrolase
Protease
Serine protease
   PTMDisulfide bond
Zymogen
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processdigestion

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentextracellular space

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionserine-type endopeptidase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform B (identifier: P29786-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform A (identifier: P29786-2)

The sequence of this isoform differs from the canonical sequence as follows:
     18-19: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2020 Potential
Propeptide21 – 277Activation peptide
PRO_0000028239
Chain28 – 254227Trypsin 3A1
PRO_0000028240

Regions

Domain28 – 253226Peptidase S1

Sites

Active site681Charge relay system By similarity
Active site1131Charge relay system By similarity
Active site2091Charge relay system By similarity
Site2031Required for specificity By similarity

Amino acid modifications

Disulfide bond53 ↔ 69 By similarity
Disulfide bond178 ↔ 194 By similarity
Disulfide bond205 ↔ 229 By similarity

Natural variations

Alternative sequence18 – 192Missing in isoform A.
VSP_035031

Experimental info

Sequence conflict14 – 174GLSQ → DSAK in CAA45714. Ref.3
Sequence conflict661A → R in CAA45714. Ref.3
Sequence conflict1361E → D in CAA45714. Ref.3
Sequence conflict2151S → F in AAM34268. Ref.2
Sequence conflict2181K → E in AAM34268. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform B [UniParc].

Last modified September 2, 2008. Version 2.
Checksum: 8127EBC9C523FAFE

FASTA25426,852
        10         20         30         40         50         60 
MNQFLFVSFC ALLGLSQVSA ATLSSGRIVG GFQIDIAEVP HQVSLQRSGR HFCGGSIISP 

        70         80         90        100        110        120 
RWVLTAAHCT TNTDPAAYTI RAGSTDRTNG GIIVKVKSVI PHPQYNGDTY NYDFSLLELD 

       130        140        150        160        170        180 
ESIGFSRSIE AIALPEASET VADGAMCTVS GWGDTKNVFE MNTLLRAVNV PSYNQAECAA 

       190        200        210        220        230        240 
ALVNVVPVTE QMICAGYAAG GKDSCQGDSG GPLVSGDKLV GVVSWGKGCA LPNLPGVYAR 

       250 
VSTVRQWIRE VSEV 

« Hide

Isoform A [UniParc].

Checksum: F3CAD99AF684B7CB
Show »

FASTA25226,666

References

« Hide 'large scale' references
[1]"Cloning and sequencing of early trypsin mRNA from Aedes aegypti 4th instar larval gut."
Borovsky D., Matthys V.S.
Submitted (FEB-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B).
Tissue: Larval gut.
[2]"Cloning, sequencing and characterization of Aedes aegypti early trypsin pre-mRNA."
Borovsky D., Peeters T.
Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE (ISOFORM B).
Tissue: Gut.
[3]"Isolation, sequencing and characterization of two cDNA clones coding for trypsin-like enzymes from the midgut of Aedes aegypti."
Kalhok S., Tabak L.M., Prosser D.E., Brook W., Downer A.E.R., White B.N.
Insect Mol. Biol. 2:71-79(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION.
Tissue: Midgut.
[4]"Genome sequence of Aedes aegypti, a major arbovirus vector."
Nene V., Wortman J.R., Lawson D., Haas B.J., Kodira C.D., Tu Z.J., Loftus B.J., Xi Z., Megy K., Grabherr M., Ren Q., Zdobnov E.M., Lobo N.F., Campbell K.S., Brown S.E., Bonaldo M.F., Zhu J., Sinkins S.P. expand/collapse author list , Hogenkamp D.G., Amedeo P., Arensburger P., Atkinson P.W., Bidwell S.L., Biedler J., Birney E., Bruggner R.V., Costas J., Coy M.R., Crabtree J., Crawford M., DeBruyn B., DeCaprio D., Eiglmeier K., Eisenstadt E., El-Dorry H., Gelbart W.M., Gomes S.L., Hammond M., Hannick L.I., Hogan J.R., Holmes M.H., Jaffe D., Johnston S.J., Kennedy R.C., Koo H., Kravitz S., Kriventseva E.V., Kulp D., Labutti K., Lee E., Li S., Lovin D.D., Mao C., Mauceli E., Menck C.F., Miller J.R., Montgomery P., Mori A., Nascimento A.L., Naveira H.F., Nusbaum C., O'Leary S.B., Orvis J., Pertea M., Quesneville H., Reidenbach K.R., Rogers Y.-H.C., Roth C.W., Schneider J.R., Schatz M., Shumway M., Stanke M., Stinson E.O., Tubio J.M.C., Vanzee J.P., Verjovski-Almeida S., Werner D., White O.R., Wyder S., Zeng Q., Zhao Q., Zhao Y., Hill C.A., Raikhel A.S., Soares M.B., Knudson D.L., Lee N.H., Galagan J., Salzberg S.L., Paulsen I.T., Dimopoulos G., Collins F.H., Bruce B., Fraser-Liggett C.M., Severson D.W.
Science 316:1718-1723(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
Strain: LVPib12.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF487426 mRNA. Translation: AAL93209.1.
AF508783 Transcribed RNA. Translation: AAM34268.1.
X64362 mRNA. Translation: CAA45714.1.
CH477469 Genomic DNA. Translation: EAT40452.1.
CH477469 Genomic DNA. Translation: EAT40453.1.
PIRTRWV3Y. S19890.
RefSeqXP_001652943.1. XM_001652893.1. [P29786-1]
XP_001652944.1. XM_001652894.1. [P29786-2]
UniGeneAae.14046.

3D structure databases

ProteinModelPortalP29786.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPSS01.130.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaAAEL007818-RB; AAEL007818-PB; AAEL007818. [P29786-1]
GeneID5569681.
KEGGaag:AaeL_AAEL007818.
VectorBaseAAEL007818. Aedes aegypti.

Phylogenomic databases

eggNOGCOG5640.
HOGENOMHOG000251820.
InParanoidQ8T637.
OMATSSFRIN.
OrthoDBEOG75B84T.
PhylomeDBP29786.

Family and domain databases

InterProIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSPR00722. CHYMOTRYPSIN.
SMARTSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMSSF50494. SSF50494. 1 hit.
PROSITEPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRY3_AEDAE
AccessionPrimary (citable) accession number: P29786
Secondary accession number(s): Q170R6, Q8MMK9, Q8T637
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: September 2, 2008
Last modified: May 14, 2014
This is version 92 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries