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P29786

- TRY3_AEDAE

UniProt

P29786 - TRY3_AEDAE

Protein

Trypsin 3A1

Gene

AAEL007818

Organism
Aedes aegypti (Yellowfever mosquito) (Culex aegypti)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 93 (01 Oct 2014)
      Sequence version 2 (02 Sep 2008)
      Previous versions | rss
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    Functioni

    Major function may be to aid in digestion of the blood meal.1 Publication

    Catalytic activityi

    Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei68 – 681Charge relay systemBy similarity
    Active sitei113 – 1131Charge relay systemBy similarity
    Sitei203 – 2031Required for specificityBy similarity
    Active sitei209 – 2091Charge relay systemBy similarity

    GO - Molecular functioni

    1. serine-type endopeptidase activity Source: InterPro

    GO - Biological processi

    1. digestion Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase, Protease, Serine protease

    Keywords - Biological processi

    Digestion

    Protein family/group databases

    MEROPSiS01.130.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Trypsin 3A1 (EC:3.4.21.4)
    Gene namesi
    ORF Names:AAEL007818
    OrganismiAedes aegypti (Yellowfever mosquito) (Culex aegypti)
    Taxonomic identifieri7159 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraNematoceraCulicoideaCulicidaeCulicinaeAediniAedesStegomyia
    ProteomesiUP000008820: Unassembled WGS sequence

    Subcellular locationi

    Secretedextracellular space 1 Publication

    GO - Cellular componenti

    1. extracellular space Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2020Sequence AnalysisAdd
    BLAST
    Propeptidei21 – 277Activation peptidePRO_0000028239
    Chaini28 – 254227Trypsin 3A1PRO_0000028240Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi53 ↔ 69PROSITE-ProRule annotation
    Disulfide bondi178 ↔ 194PROSITE-ProRule annotation
    Disulfide bondi205 ↔ 229PROSITE-ProRule annotation

    Keywords - PTMi

    Disulfide bond, Zymogen

    Expressioni

    Tissue specificityi

    Midgut.1 Publication

    Inductioni

    By blood feed.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliP29786.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini28 – 253226Peptidase S1PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase S1 family.PROSITE-ProRule annotation
    Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG5640.
    HOGENOMiHOG000251820.
    InParanoidiQ8T637.
    OMAiTSSFRIN.
    OrthoDBiEOG75B84T.
    PhylomeDBiP29786.

    Family and domain databases

    InterProiIPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view]
    PfamiPF00089. Trypsin. 1 hit.
    [Graphical view]
    PRINTSiPR00722. CHYMOTRYPSIN.
    SMARTiSM00020. Tryp_SPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF50494. SSF50494. 1 hit.
    PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform B (identifier: P29786-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MNQFLFVSFC ALLGLSQVSA ATLSSGRIVG GFQIDIAEVP HQVSLQRSGR    50
    HFCGGSIISP RWVLTAAHCT TNTDPAAYTI RAGSTDRTNG GIIVKVKSVI 100
    PHPQYNGDTY NYDFSLLELD ESIGFSRSIE AIALPEASET VADGAMCTVS 150
    GWGDTKNVFE MNTLLRAVNV PSYNQAECAA ALVNVVPVTE QMICAGYAAG 200
    GKDSCQGDSG GPLVSGDKLV GVVSWGKGCA LPNLPGVYAR VSTVRQWIRE 250
    VSEV 254
    Length:254
    Mass (Da):26,852
    Last modified:September 2, 2008 - v2
    Checksum:i8127EBC9C523FAFE
    GO
    Isoform A (identifier: P29786-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         18-19: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:252
    Mass (Da):26,666
    Checksum:iF3CAD99AF684B7CB
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti14 – 174GLSQ → DSAK in CAA45714. (PubMed:9087545)Curated
    Sequence conflicti66 – 661A → R in CAA45714. (PubMed:9087545)Curated
    Sequence conflicti136 – 1361E → D in CAA45714. (PubMed:9087545)Curated
    Sequence conflicti215 – 2151S → F in AAM34268. 1 PublicationCurated
    Sequence conflicti218 – 2181K → E in AAM34268. 1 PublicationCurated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei18 – 192Missing in isoform A. CuratedVSP_035031

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF487426 mRNA. Translation: AAL93209.1.
    AF508783 Transcribed RNA. Translation: AAM34268.1.
    X64362 mRNA. Translation: CAA45714.1.
    CH477469 Genomic DNA. Translation: EAT40452.1.
    CH477469 Genomic DNA. Translation: EAT40453.1.
    PIRiS19890. TRWV3Y.
    RefSeqiXP_001652943.1. XM_001652893.1. [P29786-1]
    XP_001652944.1. XM_001652894.1. [P29786-2]
    UniGeneiAae.14046.

    Genome annotation databases

    EnsemblMetazoaiAAEL007818-RB; AAEL007818-PB; AAEL007818. [P29786-1]
    GeneIDi5569681.
    KEGGiaag:AaeL_AAEL007818.
    VectorBaseiAAEL007818. Aedes aegypti.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF487426 mRNA. Translation: AAL93209.1 .
    AF508783 Transcribed RNA. Translation: AAM34268.1 .
    X64362 mRNA. Translation: CAA45714.1 .
    CH477469 Genomic DNA. Translation: EAT40452.1 .
    CH477469 Genomic DNA. Translation: EAT40453.1 .
    PIRi S19890. TRWV3Y.
    RefSeqi XP_001652943.1. XM_001652893.1. [P29786-1 ]
    XP_001652944.1. XM_001652894.1. [P29786-2 ]
    UniGenei Aae.14046.

    3D structure databases

    ProteinModelPortali P29786.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi S01.130.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai AAEL007818-RB ; AAEL007818-PB ; AAEL007818 . [P29786-1 ]
    GeneIDi 5569681.
    KEGGi aag:AaeL_AAEL007818.
    VectorBasei AAEL007818. Aedes aegypti.

    Phylogenomic databases

    eggNOGi COG5640.
    HOGENOMi HOG000251820.
    InParanoidi Q8T637.
    OMAi TSSFRIN.
    OrthoDBi EOG75B84T.
    PhylomeDBi P29786.

    Family and domain databases

    InterProi IPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view ]
    Pfami PF00089. Trypsin. 1 hit.
    [Graphical view ]
    PRINTSi PR00722. CHYMOTRYPSIN.
    SMARTi SM00020. Tryp_SPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50494. SSF50494. 1 hit.
    PROSITEi PS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and sequencing of early trypsin mRNA from Aedes aegypti 4th instar larval gut."
      Borovsky D., Matthys V.S.
      Submitted (FEB-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B).
      Tissue: Larval gut.
    2. "Cloning, sequencing and characterization of Aedes aegypti early trypsin pre-mRNA."
      Borovsky D., Peeters T.
      Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE (ISOFORM B).
      Tissue: Gut.
    3. "Isolation, sequencing and characterization of two cDNA clones coding for trypsin-like enzymes from the midgut of Aedes aegypti."
      Kalhok S., Tabak L.M., Prosser D.E., Brook W., Downer A.E.R., White B.N.
      Insect Mol. Biol. 2:71-79(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION.
      Tissue: Midgut.
    4. "Genome sequence of Aedes aegypti, a major arbovirus vector."
      Nene V., Wortman J.R., Lawson D., Haas B.J., Kodira C.D., Tu Z.J., Loftus B.J., Xi Z., Megy K., Grabherr M., Ren Q., Zdobnov E.M., Lobo N.F., Campbell K.S., Brown S.E., Bonaldo M.F., Zhu J., Sinkins S.P.
      , Hogenkamp D.G., Amedeo P., Arensburger P., Atkinson P.W., Bidwell S.L., Biedler J., Birney E., Bruggner R.V., Costas J., Coy M.R., Crabtree J., Crawford M., DeBruyn B., DeCaprio D., Eiglmeier K., Eisenstadt E., El-Dorry H., Gelbart W.M., Gomes S.L., Hammond M., Hannick L.I., Hogan J.R., Holmes M.H., Jaffe D., Johnston S.J., Kennedy R.C., Koo H., Kravitz S., Kriventseva E.V., Kulp D., Labutti K., Lee E., Li S., Lovin D.D., Mao C., Mauceli E., Menck C.F., Miller J.R., Montgomery P., Mori A., Nascimento A.L., Naveira H.F., Nusbaum C., O'Leary S.B., Orvis J., Pertea M., Quesneville H., Reidenbach K.R., Rogers Y.-H.C., Roth C.W., Schneider J.R., Schatz M., Shumway M., Stanke M., Stinson E.O., Tubio J.M.C., Vanzee J.P., Verjovski-Almeida S., Werner D., White O.R., Wyder S., Zeng Q., Zhao Q., Zhao Y., Hill C.A., Raikhel A.S., Soares M.B., Knudson D.L., Lee N.H., Galagan J., Salzberg S.L., Paulsen I.T., Dimopoulos G., Collins F.H., Bruce B., Fraser-Liggett C.M., Severson D.W.
      Science 316:1718-1723(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
      Strain: LVPib12.

    Entry informationi

    Entry nameiTRY3_AEDAE
    AccessioniPrimary (citable) accession number: P29786
    Secondary accession number(s): Q170R6, Q8MMK9, Q8T637
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1993
    Last sequence update: September 2, 2008
    Last modified: October 1, 2014
    This is version 93 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3