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Protein

Trypsin 3A1

Gene

AAEL007818

Organism
Aedes aegypti (Yellowfever mosquito) (Culex aegypti)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Major function may be to aid in digestion of the blood meal.1 Publication

Catalytic activityi

Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei68 – 681Charge relay systemBy similarity
Active sitei113 – 1131Charge relay systemBy similarity
Sitei203 – 2031Required for specificityBy similarity
Active sitei209 – 2091Charge relay systemBy similarity

GO - Molecular functioni

  1. serine-type endopeptidase activity Source: InterPro

GO - Biological processi

  1. digestion Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Biological processi

Digestion

Enzyme and pathway databases

BRENDAi3.4.21.4. 149.

Protein family/group databases

MEROPSiS01.130.

Names & Taxonomyi

Protein namesi
Recommended name:
Trypsin 3A1 (EC:3.4.21.4)
Gene namesi
ORF Names:AAEL007818
OrganismiAedes aegypti (Yellowfever mosquito) (Culex aegypti)
Taxonomic identifieri7159 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraNematoceraCulicoideaCulicidaeCulicinaeAediniAedesStegomyia
ProteomesiUP000008820 Componenti: Unassembled WGS sequence

Subcellular locationi

  1. Secretedextracellular space 1 Publication

GO - Cellular componenti

  1. extracellular space Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence AnalysisAdd
BLAST
Propeptidei21 – 277Activation peptidePRO_0000028239
Chaini28 – 254227Trypsin 3A1PRO_0000028240Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi53 ↔ 69PROSITE-ProRule annotation
Disulfide bondi178 ↔ 194PROSITE-ProRule annotation
Disulfide bondi205 ↔ 229PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Zymogen

Expressioni

Tissue specificityi

Midgut.1 Publication

Inductioni

By blood feed.1 Publication

Structurei

3D structure databases

ProteinModelPortaliP29786.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini28 – 253226Peptidase S1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG5640.
HOGENOMiHOG000251820.
InParanoidiP29786.
OMAiQWIREVS.
OrthoDBiEOG75B84T.
PhylomeDBiP29786.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform B (identifier: P29786-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNQFLFVSFC ALLGLSQVSA ATLSSGRIVG GFQIDIAEVP HQVSLQRSGR
60 70 80 90 100
HFCGGSIISP RWVLTAAHCT TNTDPAAYTI RAGSTDRTNG GIIVKVKSVI
110 120 130 140 150
PHPQYNGDTY NYDFSLLELD ESIGFSRSIE AIALPEASET VADGAMCTVS
160 170 180 190 200
GWGDTKNVFE MNTLLRAVNV PSYNQAECAA ALVNVVPVTE QMICAGYAAG
210 220 230 240 250
GKDSCQGDSG GPLVSGDKLV GVVSWGKGCA LPNLPGVYAR VSTVRQWIRE

VSEV
Length:254
Mass (Da):26,852
Last modified:September 2, 2008 - v2
Checksum:i8127EBC9C523FAFE
GO
Isoform A (identifier: P29786-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     18-19: Missing.

Note: No experimental confirmation available.

Show »
Length:252
Mass (Da):26,666
Checksum:iF3CAD99AF684B7CB
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti14 – 174GLSQ → DSAK in CAA45714 (PubMed:9087545).Curated
Sequence conflicti66 – 661A → R in CAA45714 (PubMed:9087545).Curated
Sequence conflicti136 – 1361E → D in CAA45714 (PubMed:9087545).Curated
Sequence conflicti215 – 2151S → F in AAM34268 (Ref. 2) Curated
Sequence conflicti218 – 2181K → E in AAM34268 (Ref. 2) Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei18 – 192Missing in isoform A. CuratedVSP_035031

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF487426 mRNA. Translation: AAL93209.1.
AF508783 Transcribed RNA. Translation: AAM34268.1.
X64362 mRNA. Translation: CAA45714.1.
CH477469 Genomic DNA. Translation: EAT40452.1.
CH477469 Genomic DNA. Translation: EAT40453.1.
PIRiS19890. TRWV3Y.
RefSeqiXP_001652943.1. XM_001652893.1. [P29786-1]
XP_001652944.1. XM_001652894.1. [P29786-2]
UniGeneiAae.14046.

Genome annotation databases

EnsemblMetazoaiAAEL007818-RB; AAEL007818-PB; AAEL007818. [P29786-1]
GeneIDi5569681.
KEGGiaag:AaeL_AAEL007818.
VectorBaseiAAEL007818. Aedes aegypti.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF487426 mRNA. Translation: AAL93209.1.
AF508783 Transcribed RNA. Translation: AAM34268.1.
X64362 mRNA. Translation: CAA45714.1.
CH477469 Genomic DNA. Translation: EAT40452.1.
CH477469 Genomic DNA. Translation: EAT40453.1.
PIRiS19890. TRWV3Y.
RefSeqiXP_001652943.1. XM_001652893.1. [P29786-1]
XP_001652944.1. XM_001652894.1. [P29786-2]
UniGeneiAae.14046.

3D structure databases

ProteinModelPortaliP29786.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiS01.130.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiAAEL007818-RB; AAEL007818-PB; AAEL007818. [P29786-1]
GeneIDi5569681.
KEGGiaag:AaeL_AAEL007818.
VectorBaseiAAEL007818. Aedes aegypti.

Phylogenomic databases

eggNOGiCOG5640.
HOGENOMiHOG000251820.
InParanoidiP29786.
OMAiQWIREVS.
OrthoDBiEOG75B84T.
PhylomeDBiP29786.

Enzyme and pathway databases

BRENDAi3.4.21.4. 149.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and sequencing of early trypsin mRNA from Aedes aegypti 4th instar larval gut."
    Borovsky D., Matthys V.S.
    Submitted (FEB-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B).
    Tissue: Larval gut.
  2. "Cloning, sequencing and characterization of Aedes aegypti early trypsin pre-mRNA."
    Borovsky D., Peeters T.
    Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE (ISOFORM B).
    Tissue: Gut.
  3. "Isolation, sequencing and characterization of two cDNA clones coding for trypsin-like enzymes from the midgut of Aedes aegypti."
    Kalhok S., Tabak L.M., Prosser D.E., Brook W., Downer A.E.R., White B.N.
    Insect Mol. Biol. 2:71-79(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION.
    Tissue: Midgut.
  4. "Genome sequence of Aedes aegypti, a major arbovirus vector."
    Nene V., Wortman J.R., Lawson D., Haas B.J., Kodira C.D., Tu Z.J., Loftus B.J., Xi Z., Megy K., Grabherr M., Ren Q., Zdobnov E.M., Lobo N.F., Campbell K.S., Brown S.E., Bonaldo M.F., Zhu J., Sinkins S.P.
    , Hogenkamp D.G., Amedeo P., Arensburger P., Atkinson P.W., Bidwell S.L., Biedler J., Birney E., Bruggner R.V., Costas J., Coy M.R., Crabtree J., Crawford M., DeBruyn B., DeCaprio D., Eiglmeier K., Eisenstadt E., El-Dorry H., Gelbart W.M., Gomes S.L., Hammond M., Hannick L.I., Hogan J.R., Holmes M.H., Jaffe D., Johnston S.J., Kennedy R.C., Koo H., Kravitz S., Kriventseva E.V., Kulp D., Labutti K., Lee E., Li S., Lovin D.D., Mao C., Mauceli E., Menck C.F., Miller J.R., Montgomery P., Mori A., Nascimento A.L., Naveira H.F., Nusbaum C., O'Leary S.B., Orvis J., Pertea M., Quesneville H., Reidenbach K.R., Rogers Y.-H.C., Roth C.W., Schneider J.R., Schatz M., Shumway M., Stanke M., Stinson E.O., Tubio J.M.C., Vanzee J.P., Verjovski-Almeida S., Werner D., White O.R., Wyder S., Zeng Q., Zhao Q., Zhao Y., Hill C.A., Raikhel A.S., Soares M.B., Knudson D.L., Lee N.H., Galagan J., Salzberg S.L., Paulsen I.T., Dimopoulos G., Collins F.H., Bruce B., Fraser-Liggett C.M., Severson D.W.
    Science 316:1718-1723(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
    Strain: LVPib12.

Entry informationi

Entry nameiTRY3_AEDAE
AccessioniPrimary (citable) accession number: P29786
Secondary accession number(s): Q170R6, Q8MMK9, Q8T637
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: September 2, 2008
Last modified: April 1, 2015
This is version 96 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.