Reviewed, UniProtKB/Swiss-Prot P29786 (TRY3_AEDAE)

Names and origin

Protein names

Recommended name:
    Trypsin 3A1
    EC=3.4.21.4

Gene names

ORF Names:

AAEL007818

Organism

Aedes aegypti (Yellowfever mosquito) (Culex aegypti)

Taxonomic identifier

7159 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Nematocera › Culicoidea › Culicidae › Culicinae › Culicini › Aedes › Stegomyia

Protein attributes

Sequence length	254 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Major function may be to aid in digestion of the blood meal. Ref.3
Catalytic activity	Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.
Subcellular location	Secreted › extracellular space. Ref.3
Tissue specificity	Midgut. Ref.3
Induction	By blood feed. Ref.3
Sequence similarities	Belongs to the peptidase S1 family. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Biological process	Digestion
Cellular component	Secreted
Coding sequence diversity	Alternative splicing
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Zymogen
Gene Ontology (GO)
Biological process	digestion Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform B (identifier: P29786-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform A (identifier: P29786-2)

The sequence of this isoform differs from the canonical sequence as follows:
     18-19: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential
	Propeptide	21 – 27	7	Activation peptide		PRO_0000028239
	Chain	28 – 254	227	Trypsin 3A1		PRO_0000028240
Regions
	Domain	28 – 253	226	Peptidase S1
Sites
	Active site	68	1	Charge relay system By similarity
	Active site	113	1	Charge relay system By similarity
	Active site	209	1	Charge relay system By similarity
	Site	203	1	Required for specificity By similarity
Amino acid modifications
	Disulfide bond	53 ↔ 69		By similarity
	Disulfide bond	178 ↔ 194		By similarity
	Disulfide bond	205 ↔ 229		By similarity
Natural variations
	Alternative sequence	18 – 19	2	Missing in isoform A.		VSP_035031
Experimental info
	Sequence conflict	14 – 17	4	GLSQ → DSAK in CAA45714. Ref.3
	Sequence conflict	66	1	A → R in CAA45714. Ref.3
	Sequence conflict	136	1	E → D in CAA45714. Ref.3
	Sequence conflict	215	1	S → F in AAM34268. Ref.2
	Sequence conflict	218	1	K → E in AAM34268. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform B [UniParc]. Last modified September 2, 2008. Version 2. Checksum: 8127EBC9C523FAFE	FASTA	254	26,852
	10 20 30 40 50 60 MNQFLFVSFC ALLGLSQVSA ATLSSGRIVG GFQIDIAEVP HQVSLQRSGR HFCGGSIISP 70 80 90 100 110 120 RWVLTAAHCT TNTDPAAYTI RAGSTDRTNG GIIVKVKSVI PHPQYNGDTY NYDFSLLELD 130 140 150 160 170 180 ESIGFSRSIE AIALPEASET VADGAMCTVS GWGDTKNVFE MNTLLRAVNV PSYNQAECAA 190 200 210 220 230 240 ALVNVVPVTE QMICAGYAAG GKDSCQGDSG GPLVSGDKLV GVVSWGKGCA LPNLPGVYAR 250 VSTVRQWIRE VSEV
	Isoform A. Checksum: F3CAD99AF684B7CB	FASTA	252	26,666
	10 20 30 40 50 60 MNQFLFVSFC ALLGLSQAAT LSSGRIVGGF QIDIAEVPHQ VSLQRSGRHF CGGSIISPRW 70 80 90 100 110 120 VLTAAHCTTN TDPAAYTIRA GSTDRTNGGI IVKVKSVIPH PQYNGDTYNY DFSLLELDES 130 140 150 160 170 180 IGFSRSIEAI ALPEASETVA DGAMCTVSGW GDTKNVFEMN TLLRAVNVPS YNQAECAAAL 190 200 210 220 230 240 VNVVPVTEQM ICAGYAAGGK DSCQGDSGGP LVSGDKLVGV VSWGKGCALP NLPGVYARVS 250 TVRQWIREVS EV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and sequencing of early trypsin mRNA from Aedes aegypti 4th instar larval gut." Borovsky D., Matthys V.S. Submitted (FEB-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B). Tissue: Larval gut.
[2]	"Cloning, sequencing and characterization of Aedes aegypti early trypsin pre-mRNA." Borovsky D., Peeters T. Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE (ISOFORM B). Tissue: Gut.
[3]	"Isolation, sequencing and characterization of two cDNA clones coding for trypsin-like enzymes from the midgut of Aedes aegypti." Kalhok S., Tabak L.M., Prosser D.E., Brook W., Downer A.E.R., White B.N. Insect Mol. Biol. 2:71-79(1993) [PubMed: 9087545] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION. Tissue: Midgut.
[4]	"Genome sequence of Aedes aegypti, a major arbovirus vector." Nene V., Wortman J.R., Lawson D., Haas B.J., Kodira C.D., Tu Z.J., Loftus B.J., Xi Z., Megy K., Grabherr M., Ren Q., Zdobnov E.M., Lobo N.F., Campbell K.S., Brown S.E., Bonaldo M.F., Zhu J., Sinkins S.P. , Hogenkamp D.G., Amedeo P., Arensburger P., Atkinson P.W., Bidwell S.L., Biedler J., Birney E., Bruggner R.V., Costas J., Coy M.R., Crabtree J., Crawford M., DeBruyn B., DeCaprio D., Eiglmeier K., Eisenstadt E., El-Dorry H., Gelbart W.M., Gomes S.L., Hammond M., Hannick L.I., Hogan J.R., Holmes M.H., Jaffe D., Johnston S.J., Kennedy R.C., Koo H., Kravitz S., Kriventseva E.V., Kulp D., Labutti K., Lee E., Li S., Lovin D.D., Mao C., Mauceli E., Menck C.F., Miller J.R., Montgomery P., Mori A., Nascimento A.L., Naveira H.F., Nusbaum C., O'Leary S.B., Orvis J., Pertea M., Quesneville H., Reidenbach K.R., Rogers Y.-H.C., Roth C.W., Schneider J.R., Schatz M., Shumway M., Stanke M., Stinson E.O., Tubio J.M.C., Vanzee J.P., Verjovski-Almeida S., Werner D., White O.R., Wyder S., Zeng Q., Zhao Q., Zhao Y., Hill C.A., Raikhel A.S., Soares M.B., Knudson D.L., Lee N.H., Galagan J., Salzberg S.L., Paulsen I.T., Dimopoulos G., Collins F.H., Bruce B., Fraser-Liggett C.M., Severson D.W. Science 316:1718-1723(2007) [PubMed: 17510324] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING. Strain: Liverpool.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF487426 mRNA. Translation: AAL93209.1. AF508783 Transcribed RNA. Translation: AAM34268.1. X64362 mRNA. Translation: CAA45714.1. CH477469 Genomic DNA. Translation: EAT40452.1. CH477469 Genomic DNA. Translation: EAT40453.1.
PIR	TRWV3Y. S19890.
RefSeq	XP_001652943.1. XP_001652944.1.
UniGene	Aae.20570
3D structure databases
HSSP	HSSP built from PDB template 1EZX based on UniProtKB P00760.
ModBase	Search...
Protein family/group databases
MEROPS	S01.130.
Genome annotation databases
Ensembl	AAEL007818. Aedes aegypti. [Contig view]
GeneID	5569681.
KEGG	aag:AaeL_AAEL007818.
Enzyme and pathway databases
BRENDA	3.4.21.4. 1026.
Family and domain databases
InterPro	IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRY3_AEDAE

Accession

Primary (citable) accession number: P29786
Secondary accession number(s): Q170R6, Q8MMK9, Q8T637

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	September 2, 2008
Last modified:	June 16, 2009
This is version 62 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)