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P29781

- RMLD_STRGR

UniProt

P29781 - RMLD_STRGR

Protein

dTDP-4-dehydrorhamnose reductase

Gene

strL

Organism
Streptomyces griseus
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 68 (01 Oct 2014)
      Sequence version 1 (01 Apr 1993)
      Previous versions | rss
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    Functioni

    Involved in the biosynthesis of the streptose moiety of streptomycin. Catalyzes the reduction of dTDP-6-deoxy-L-lyxo-4-hexulose to yield dTDP-L-rhamnose. RmlD uses NADH and NADPH nearly equally well By similarity.By similarity

    Catalytic activityi

    dTDP-beta-L-rhamnose + NADP+ = dTDP-4-dehydro-beta-L-rhamnose + NADPH.

    Cofactori

    Binds 1 magnesium ion per monomer.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei157 – 1571Substrate; via amide nitrogenBy similarity
    Binding sitei227 – 2271SubstrateBy similarity
    Binding sitei259 – 2591SubstrateBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi13 – 197NADPSequence Analysis
    Nucleotide bindingi13 – 175NADBy similarity
    Nucleotide bindingi17 – 182NADPBy similarity
    Nucleotide bindingi36 – 372NADBy similarity
    Nucleotide bindingi42 – 432NAD/NADPBy similarity
    Nucleotide bindingi65 – 684NADBy similarity
    Nucleotide bindingi66 – 683NADPBy similarity
    Nucleotide bindingi131 – 1355NAD/NADPBy similarity

    GO - Molecular functioni

    1. dTDP-4-dehydrorhamnose reductase activity Source: UniProtKB

    GO - Biological processi

    1. dTDP-rhamnose biosynthetic process Source: UniProtKB-UniPathway
    2. extracellular polysaccharide biosynthetic process Source: UniProtKB
    3. streptomycin biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Antibiotic biosynthesis, Streptomycin biosynthesis

    Keywords - Ligandi

    NADP

    Enzyme and pathway databases

    UniPathwayiUPA00066.
    UPA00124.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    dTDP-4-dehydrorhamnose reductase (EC:1.1.1.133)
    Alternative name(s):
    dTDP-4-keto-L-rhamnose reductase
    dTDP-6-deoxy-L-lyxo-4-hexulose reductase
    dTDP-6-deoxy-L-mannose dehydrogenase
    dTDP-L-rhamnose synthase
    Gene namesi
    Name:strL
    OrganismiStreptomyces griseus
    Taxonomic identifieri1911 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 304304dTDP-4-dehydrorhamnose reductasePRO_0000207989Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP29781.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni107 – 1082Substrate bindingBy similarity

    Sequence similaritiesi

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR005913. dTDP_dehydrorham_reduct.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PfamiPF04321. RmlD_sub_bind. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR01214. rmlD. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P29781-1 [UniParc]FASTAAdd to Basket

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    MSPYPRPRWL VTGASGMLGR ELTPLLDRRG AAVTALGRGH LDITDGAAVR    50
    SAVAEHRPAV VVNCAAWTAV DEAESEPALA MAVNGEGPRH LAQACRAVGA 100
    VLLQLSTDYV FPGSGGRPYR EDHPTGPRTV YGCTKRAGER AVLEVLPDTG 150
    YIVRTAWLYG AGGPNFVAKM IRLEADEDTV LVVDDQHGQP TWTADLADRL 200
    AALGAAALAG TAPAGIYHAT NTGGTTWNAL APETFRLLGA DPARVRPTTS 250
    LALARPAVRP RYSVLDQSRW KAAGLEPLRH WRAALTESFP ALCGRAGRPV 300
    PGPR 304
    Length:304
    Mass (Da):32,215
    Last modified:April 1, 1993 - v1
    Checksum:i7594F25D3F7ED0CB
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X62567 Genomic DNA. Translation: CAA44443.1.
    PIRiS18618. SYSMPG.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X62567 Genomic DNA. Translation: CAA44443.1 .
    PIRi S18618. SYSMPG.

    3D structure databases

    ProteinModelPortali P29781.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00066 .
    UPA00124 .

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    InterProi IPR005913. dTDP_dehydrorham_reduct.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    Pfami PF04321. RmlD_sub_bind. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR01214. rmlD. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Genetics of streptomycin production in Streptomyces griseus: molecular structure and putative function of genes strELMB2N."
      Pissowotzki K., Mansouri K., Piepersberg W.
      Mol. Gen. Genet. 231:113-123(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION IN BIOSYNTHESIS OF THE STREPTOSE MOIETY OF STREPTOMYCIN.
      Strain: N2-3-11.

    Entry informationi

    Entry nameiRMLD_STRGR
    AccessioniPrimary (citable) accession number: P29781
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1993
    Last sequence update: April 1, 1993
    Last modified: October 1, 2014
    This is version 68 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3