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P29781

- RMLD_STRGR

UniProt

P29781 - RMLD_STRGR

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Protein
dTDP-4-dehydrorhamnose reductase
Gene
strL
Organism
Streptomyces griseus
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in the biosynthesis of the streptose moiety of streptomycin. Catalyzes the reduction of dTDP-6-deoxy-L-lyxo-4-hexulose to yield dTDP-L-rhamnose. RmlD uses NADH and NADPH nearly equally well By similarity.1 Publication

Catalytic activityi

dTDP-beta-L-rhamnose + NADP+ = dTDP-4-dehydro-beta-L-rhamnose + NADPH.

Cofactori

Binds 1 magnesium ion per monomer By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei157 – 1571Substrate; via amide nitrogen By similarity
Binding sitei227 – 2271Substrate By similarity
Binding sitei259 – 2591Substrate By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi13 – 197NADP Reviewed prediction
Nucleotide bindingi13 – 175NAD By similarity
Nucleotide bindingi17 – 182NADP By similarity
Nucleotide bindingi36 – 372NAD By similarity
Nucleotide bindingi42 – 432NAD/NADP By similarity
Nucleotide bindingi65 – 684NAD By similarity
Nucleotide bindingi66 – 683NADP By similarity
Nucleotide bindingi131 – 1355NAD/NADP By similarity

GO - Molecular functioni

  1. dTDP-4-dehydrorhamnose reductase activity Source: UniProtKB

GO - Biological processi

  1. dTDP-rhamnose biosynthetic process Source: UniProtKB-UniPathway
  2. extracellular polysaccharide biosynthetic process Source: UniProtKB
  3. streptomycin biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Antibiotic biosynthesis, Streptomycin biosynthesis

Keywords - Ligandi

NADP

Enzyme and pathway databases

UniPathwayiUPA00066.
UPA00124.

Names & Taxonomyi

Protein namesi
Recommended name:
dTDP-4-dehydrorhamnose reductase (EC:1.1.1.133)
Alternative name(s):
dTDP-4-keto-L-rhamnose reductase
dTDP-6-deoxy-L-lyxo-4-hexulose reductase
dTDP-6-deoxy-L-mannose dehydrogenase
dTDP-L-rhamnose synthase
Gene namesi
Name:strL
OrganismiStreptomyces griseus
Taxonomic identifieri1911 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 304304dTDP-4-dehydrorhamnose reductase
PRO_0000207989Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.

Structurei

3D structure databases

ProteinModelPortaliP29781.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni107 – 1082Substrate binding By similarity

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR005913. dTDP_dehydrorham_reduct.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF04321. RmlD_sub_bind. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01214. rmlD. 1 hit.

Sequencei

Sequence statusi: Complete.

P29781-1 [UniParc]FASTAAdd to Basket

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MSPYPRPRWL VTGASGMLGR ELTPLLDRRG AAVTALGRGH LDITDGAAVR    50
SAVAEHRPAV VVNCAAWTAV DEAESEPALA MAVNGEGPRH LAQACRAVGA 100
VLLQLSTDYV FPGSGGRPYR EDHPTGPRTV YGCTKRAGER AVLEVLPDTG 150
YIVRTAWLYG AGGPNFVAKM IRLEADEDTV LVVDDQHGQP TWTADLADRL 200
AALGAAALAG TAPAGIYHAT NTGGTTWNAL APETFRLLGA DPARVRPTTS 250
LALARPAVRP RYSVLDQSRW KAAGLEPLRH WRAALTESFP ALCGRAGRPV 300
PGPR 304
Length:304
Mass (Da):32,215
Last modified:April 1, 1993 - v1
Checksum:i7594F25D3F7ED0CB
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X62567 Genomic DNA. Translation: CAA44443.1.
PIRiS18618. SYSMPG.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X62567 Genomic DNA. Translation: CAA44443.1 .
PIRi S18618. SYSMPG.

3D structure databases

ProteinModelPortali P29781.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00066 .
UPA00124 .

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
InterProi IPR005913. dTDP_dehydrorham_reduct.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
Pfami PF04321. RmlD_sub_bind. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01214. rmlD. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Genetics of streptomycin production in Streptomyces griseus: molecular structure and putative function of genes strELMB2N."
    Pissowotzki K., Mansouri K., Piepersberg W.
    Mol. Gen. Genet. 231:113-123(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION IN BIOSYNTHESIS OF THE STREPTOSE MOIETY OF STREPTOMYCIN.
    Strain: N2-3-11.

Entry informationi

Entry nameiRMLD_STRGR
AccessioniPrimary (citable) accession number: P29781
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: December 11, 2013
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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