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Protein

Sialidase

Gene

nanH

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cleaves the terminal sialic acid (N-acetyl neuraminic acid) from carbohydrate chains in glycoproteins providing free sialic acid which can be used as carbon and energy sources. Sialidases have been suggested to be pathogenic factors in microbial infections.

Catalytic activityi

Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei37Substrate1
Active sitei62Proton acceptorBy similarity1
Binding sitei246Substrate1
Binding sitei309Substrate1
Active sitei342NucleophileBy similarity1
Active sitei3611

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Protein family/group databases

CAZyiGH33. Glycoside Hydrolase Family 33.

Names & Taxonomyi

Protein namesi
Recommended name:
Sialidase (EC:3.2.1.18)
Alternative name(s):
N-acylneuraminate glycohydrolase
Neuraminidase
Short name:
NANase
STNA
Gene namesi
Name:nanH
Ordered Locus Names:STM0928
OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Taxonomic identifieri99287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeSalmonella
Proteomesi
  • UP000001014 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00002089092 – 382SialidaseAdd BLAST381

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi42 ↔ 103

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP29768.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

STRINGi99287.STM0928.

Structurei

Secondary structure

1382
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi5 – 9Combined sources5
Helixi29 – 31Combined sources3
Beta strandi35 – 43Combined sources9
Beta strandi49 – 58Combined sources10
Beta strandi62 – 64Combined sources3
Beta strandi66 – 78Combined sources13
Beta strandi81 – 86Combined sources6
Turni93 – 95Combined sources3
Beta strandi97 – 108Combined sources12
Beta strandi111 – 123Combined sources13
Helixi128 – 130Combined sources3
Beta strandi133 – 136Combined sources4
Beta strandi141 – 149Combined sources9
Beta strandi155 – 157Combined sources3
Helixi161 – 168Combined sources8
Beta strandi170 – 176Combined sources7
Beta strandi178 – 180Combined sources3
Beta strandi189 – 197Combined sources9
Beta strandi204 – 219Combined sources16
Beta strandi232 – 237Combined sources6
Beta strandi240 – 245Combined sources6
Beta strandi248 – 250Combined sources3
Beta strandi254 – 261Combined sources8
Turni267 – 271Combined sources5
Beta strandi283 – 289Combined sources7
Beta strandi292 – 300Combined sources9
Beta strandi312 – 317Combined sources6
Turni319 – 321Combined sources3
Beta strandi324 – 331Combined sources8
Beta strandi343 – 350Combined sources8
Beta strandi353 – 362Combined sources10
Beta strandi365 – 370Combined sources6
Helixi372 – 374Combined sources3
Helixi375 – 379Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1DILX-ray1.90A2-382[»]
1DIMX-ray1.60A2-382[»]
2SILX-ray1.60A2-382[»]
2SIMX-ray1.60A2-382[»]
3SILX-ray1.05A4-382[»]
ProteinModelPortaliP29768.
SMRiP29768.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP29768.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati71 – 82BNR 1Add BLAST12
Repeati145 – 156BNR 2Add BLAST12
Repeati210 – 220BNR 3Add BLAST11
Repeati254 – 265BNR 4Add BLAST12

Sequence similaritiesi

Belongs to the glycosyl hydrolase 33 family.Curated
Contains 4 BNR repeats.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG4105W5A. Bacteria.
COG4409. LUCA.
KOiK01186.
OMAiYEANGNI.

Family and domain databases

Gene3Di2.120.10.10. 1 hit.
InterProiIPR026856. Sialidase_fam.
IPR008377. Sialidase_trypan.
IPR011040. Sialidases.
[Graphical view]
PANTHERiPTHR10628. PTHR10628. 1 hit.
PfamiPF13859. BNR_3. 1 hit.
[Graphical view]
PRINTSiPR01803. TCSIALIDASE.
SUPFAMiSSF50939. SSF50939. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P29768-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTVEKSVVFK AEGEHFTDQK GNTIVGSGSG GTTKYFRIPA MCTTSKGTIV
60 70 80 90 100
VFADARHNTA SDQSFIDTAA ARSTDGGKTW NKKIAIYNDR VNSKLSRVMD
110 120 130 140 150
PTCIVANIQG RETILVMVGK WNNNDKTWGA YRDKAPDTDW DLVLYKSTDD
160 170 180 190 200
GVTFSKVETN IHDIVTKNGT ISAMLGGVGS GLQLNDGKLV FPVQMVRTKN
210 220 230 240 250
ITTVLNTSFI YSTDGITWSL PSGYCEGFGS ENNIIEFNAS LVNNIRNSGL
260 270 280 290 300
RRSFETKDFG KTWTEFPPMD KKVDNRNHGV QGSTITIPSG NKLVAAHSSA
310 320 330 340 350
QNKNNDYTRS DISLYAHNLY SGEVKLIDDF YPKVGNASGA GYSCLSYRKN
360 370 380
VDKETLYVVY EANGSIEFQD LSRHLPVIKS YN
Length:382
Mass (Da):42,073
Last modified:January 23, 2007 - v4
Checksum:iC15515CF9C15597E
GO

Sequence cautioni

The sequence AAL19864 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti329D → A in AAA27168 (PubMed:1602967).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M55342 Genomic DNA. Translation: AAA27168.1.
AE006468 Genomic DNA. Translation: AAL19864.1. Different initiation.
RefSeqiNP_459905.1. NC_003197.1.
WP_010989000.1. NC_003197.1.

Genome annotation databases

EnsemblBacteriaiAAL19864; AAL19864; STM0928.
GeneIDi1252447.
KEGGistm:STM0928.
PATRICi32380261. VBISalEnt20916_0978.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M55342 Genomic DNA. Translation: AAA27168.1.
AE006468 Genomic DNA. Translation: AAL19864.1. Different initiation.
RefSeqiNP_459905.1. NC_003197.1.
WP_010989000.1. NC_003197.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1DILX-ray1.90A2-382[»]
1DIMX-ray1.60A2-382[»]
2SILX-ray1.60A2-382[»]
2SIMX-ray1.60A2-382[»]
3SILX-ray1.05A4-382[»]
ProteinModelPortaliP29768.
SMRiP29768.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi99287.STM0928.

Protein family/group databases

CAZyiGH33. Glycoside Hydrolase Family 33.

Proteomic databases

PaxDbiP29768.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL19864; AAL19864; STM0928.
GeneIDi1252447.
KEGGistm:STM0928.
PATRICi32380261. VBISalEnt20916_0978.

Phylogenomic databases

eggNOGiENOG4105W5A. Bacteria.
COG4409. LUCA.
KOiK01186.
OMAiYEANGNI.

Miscellaneous databases

EvolutionaryTraceiP29768.

Family and domain databases

Gene3Di2.120.10.10. 1 hit.
InterProiIPR026856. Sialidase_fam.
IPR008377. Sialidase_trypan.
IPR011040. Sialidases.
[Graphical view]
PANTHERiPTHR10628. PTHR10628. 1 hit.
PfamiPF13859. BNR_3. 1 hit.
[Graphical view]
PRINTSiPR01803. TCSIALIDASE.
SUPFAMiSSF50939. SSF50939. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNANH_SALTY
AccessioniPrimary (citable) accession number: P29768
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 139 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.