P29767 (NANH_CLOSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Sialidase EC=3.2.1.18 Alternative name(s): Neuraminidase
Organism	Clostridium septicum
Taxonomic identifier	1504 [NCBI]
Taxonomic lineage	Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	1014 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Sialidases have been suggested to be pathogenic factors in microbial infections.
Catalytic activity	Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.
Subcellular location	Secreted.
Sequence similarities	Belongs to the glycosyl hydrolase 33 family. Contains 4 BNR repeats. Contains 1 F5/8 type C domain.

Ontologies

Keywords
Cellular component	Secreted
Domain	Repeat Signal
Molecular function	Glycosidase Hydrolase
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro cell adhesion Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	exo-alpha-(2->3)-sialidase activity Inferred from electronic annotation. Source: EC exo-alpha-(2->6)-sialidase activity Inferred from electronic annotation. Source: EC exo-alpha-(2->8)-sialidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 26	26	Potential
	Chain	27 – 1014	988	Sialidase		PRO_0000012031
Regions
	Domain	39 – 186	148	F5/8 type C
	Repeat	431 – 442	12	BNR 1
	Repeat	563 – 574	12	BNR 2
	Repeat	627 – 638	12	BNR 3
	Repeat	700 – 711	12	BNR 4
Sites
	Active site	421	1	Proton acceptor By similarity
	Active site	912	1	Nucleophile By similarity
	Binding site	396	1	Substrate By similarity
	Binding site	688	1	Substrate By similarity
	Binding site	873	1	Substrate Potential

Sequences

	Sequence		Length	Mass (Da)
	P29767 [UniParc]. Last modified April 1, 1993. Version 1. Checksum: C4F49233473A2FAD	FASTA	1,014	110,653
	10 20 30 40 50 60 MNKKKIMSIL VSAFLITNLS SNIIFADIKE NVYINQYSEG NRSQPIAEKL VPRSEIQASA 70 80 90 100 110 120 TSALTGEGPE KAIDGNTSTL WHTPWAGVDI QINPQSLTLK LGKTRNISSI CVTPRQEGTN 130 140 150 160 170 180 GMITDYKIYS GDDVIAEGKW KSDSSDKYVV FDNPISTDNI RIEAISTVGD ENNKHASIAE 190 200 210 220 230 240 VEVYELADTP VKLAESNNKV INNGNGGNYE GDISEISLLE EGTAIIRFTN NGSSLFSISN 250 260 270 280 290 300 NERTNEHFHV YINGGAIGYE LRKQSGNLAT GSVNKALNAG INTIAFKAEK GKGYSIYLNG 310 320 330 340 350 360 EKILTSSSIT ANFLSTLEGL NTLSLGKTDR PSGSNEYNFT GEIDFFELYS KPLADRYLKE 370 380 390 400 410 420 RTGETTSKDL PFPEGAVKTE PVDIFTPGEL GSNNFRIPAL YTTKDGTVLA SIDVRKGGGH 430 440 450 460 470 480 DAPNNIDTGI KRSTDGGVTW DEGKIILDYP GASSAIDTSL LQDDETGRIF LIVTHFAEGY 490 500 510 520 530 540 GFGNSKTGSG YVEIEGKRYL KLLGANDTIY TVREGVVYDS NGEATNYTVD NNNELYENGN 550 560 570 580 590 600 RIGNVLLSNS PLKVMGTSFL SLIYSDDDGQ TWSDPIDLNK EVKTDWMRFL GTGPGKGHQI 610 620 630 640 650 660 KTGRYAGRLL FPVYLTNASG FQSSAVIYSD DNGATWNIGE TATDGRLMDN GDRASAETIT 670 680 690 700 710 720 TNTSGGVGQL TECQVVEMPN GQLKMFMRNT GGNSGRVRIA TSFDGGATWE DDVVRDENIK 730 740 750 760 770 780 EPYCQLSVIN YSQKIDGKDA IIFAIPDANY PNRVNGTVRV GLITENGSYE NGEPRYDIEW 790 800 810 820 830 840 RYNKVVAPGT YGYSCLSEMP NGEIGLFYEG RGSRQMSFTR MNIDYLKADL LQDVPAANIK 850 860 870 880 890 900 SYTTNSENNI YDPGDKISLN VTFDQTVSLI GDRTITADIG GKEVLLTLAN SKGGSEYTFE 910 920 930 940 950 960 GTVPADISNG NYTITIKGKS GLKIVNVVNK VTDITEDRNT GLNVQVGEEV QSVDKTLLQD 970 980 990 1000 1010 LVDSTSNLIK EDYTEESWIL YEKALEVANK FLVNEIAVQE EVDAAKPTLE NAYK

References

[1]	"The sialidase gene from Clostridium septicum: cloning, sequencing, expression in Escherichia coli and identification of conserved sequences in sialidases and other proteins." Rothe B., Rothe B., Roggentin P., Schauer R. Mol. Gen. Genet. 226:190-197(1991) [PubMed: 2034213] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: NC 0054714.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X63266 Genomic DNA. Translation: CAA44916.1.
PIR	NMCLSS. S15994.
3D structure databases
ProteinModelPortal	P29767.
ModBase	Search...
Protein family/group databases
CAZy	CBM32. Carbohydrate-Binding Module Family 32. GH33. Glycoside Hydrolase Family 33.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR002860. BNR_rpt. IPR000421. Coagulation_fac_5/8-C_type_dom. IPR008985. ConA-like_lec_gl. IPR013320. ConA-like_subgrp. IPR008979. Galactose-bd-like. IPR004124. Glyco_hydro_33_N. IPR011040. Neuraminidase. IPR023364. Trans_sialidase_dom3. [Graphical view]
Gene3D	G3DSA:2.60.120.200. ConA_like_subgrp. 1 hit. G3DSA:2.40.220.10. G3DSA:2.40.220.10. 1 hit. G3DSA:2.120.10.10. Neuraminidase. 2 hits.
Pfam	PF02012. BNR. 4 hits. PF00754. F5_F8_type_C. 1 hit. PF02973. Sialidase. 1 hit. [Graphical view]
SUPFAM	SSF49899. ConA_like_lec_gl. 1 hit. SSF49785. Gal_bind_like. 1 hit. SSF50939. Sialidase. 1 hit.
PROSITE	PS50022. FA58C_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NANH_CLOSE

Accession

Primary (citable) accession number: P29767

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	January 25, 2012
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program