P29761 (AMYG_CLOS0) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glucoamylase
EC=3.2.1.3

Alternative name(s):
1,4-alpha-D-glucan glucohydrolase
Glucan 1,4-alpha-glucosidase

Gene names

Name:

cga

Organism

Clostridium sp. (strain G0005)

Taxonomic identifier

72582 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	702 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	CGA has typical kinetic properties for a glucoamylase, but this bacterial enzyme had higher isomaltose-hydrolyzing activity than other eukaryotic glucoamylases.
Catalytic activity	Hydrolysis of terminal (1->4)-linked alpha-D-glucose residues successively from non-reducing ends of the chains with release of beta-D-glucose.
Subcellular location	Cell membrane; Lipid-anchor.
Miscellaneous	High activity towards 1,6-glycosidic bonds.
Sequence similarities	Belongs to the glycosyl hydrolase 15 family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Polysaccharide degradation
Cellular component	Cell membrane Membrane
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Lipoprotein Palmitate
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	polysaccharide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	carbohydrate binding Inferred from electronic annotation. Source: InterPro glucan 1,4-alpha-glucosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Chain	22 – 702	681	Glucoamylase		PRO_0000001481
Sites
	Active site	452	1	Proton acceptor By similarity
	Active site	455	1	Proton donor By similarity
	Binding site	342	1	Substrate By similarity
Amino acid modifications
	Lipidation	22	1	N-palmitoyl cysteine Probable
	Lipidation	22	1	S-diacylglycerol cysteine Probable

Sequences

	Sequence		Length	Mass (Da)
	P29761 [UniParc]. Last modified April 1, 1993. Version 1. Checksum: 955EB4D0AD569546	FASTA	702	78,658
	10 20 30 40 50 60 MSRKLIKYLP LLVLASSVLS GCSNNVSSIK IDRFNNISAV NGPGEEDTWA SAQKQGVGTA 70 80 90 100 110 120 NNYVSKVWFT LANGAISEVY YPTIDTADVK EIKFIVTDGK SFVSDETKDT ISKVEKFTDK 130 140 150 160 170 180 SLGYKLVNTD KKGRYRITKE IFTDVKRNSL IMKAKFEALE GSIHDYKLYL AYDPHIKNQG 190 200 210 220 230 240 SYNEGYVIKA NNNEMLMAKR DNVYTALSSN IGWKGYSIGY YKVNDIMTDL DENKQMTKHY 250 260 270 280 290 300 DSARGNIIEG AEIDLKKNSQ FEIVLSFGNS EDEAVKASIE TLSENYDSLK SAYIDEWEKY 310 320 330 340 350 360 CNSLNNFNGK ANSLYYNSMM ILKASEDKTN KGAYIASLSI PWGDGQGDDN TGGYHLVWSR 370 380 390 400 410 420 DLYHVANAFI AAGDVDSANR SLDYLAKVVK DNGMIPQNTW ISGKPYWTGI QLDEQADPII 430 440 450 460 470 480 LSYRLRRYDL YDSLVKPLAD FIIKMGPKTG QERWEEIGGY SPATMAAEVA GLTCAAYIAE 490 500 510 520 530 540 QNKDYESAQK YQEKADNWQK LIDNLTYTEH GPLENGQYYI RIAGLPDPNA DFTISIANGG 550 560 570 580 590 600 GVYDQKEIVD PSFLELVRLG VKSPDDPKIL NTLRVVDSTI KVDTPKGPSW YRYNHDGYGE 610 620 630 640 650 660 PSKTELYHGA GKGRLWPLLT GERGMYEIAA GKDATPYLKA MENFANEGGI ISEQVWEDTG 670 680 690 700 LPTDSASPLN WAHAEYVVLF PSNIEHKVLD MPDIVYKRYV AK

References

[1]

"Molecular cloning of a glucoamylase gene from a thermophilic Clostridium and kinetics of the cloned enzyme." Ohnishi H., Kitamura H., Minowa T., Sakai H., Ohta T. Eur. J. Biochem. 207:413-418(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D12818 Genomic DNA. Translation: BAA02251.1.
3D structure databases
ProteinModelPortal	P29761.
SMR	P29761. Positions 28-701.
ModBase	Search...
Protein family/group databases
CAZy	GH15. Glycoside Hydrolase Family 15.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
SABIO-RK	P29761.
Family and domain databases
Gene3D	1.50.10.10. 1 hit. 2.70.98.10. 1 hit.
InterPro	IPR008928. 6-hairpin_glycosidase-like. IPR012341. 6hp_glycosidase. IPR011013. Gal_mutarotase_SF_dom. IPR000165. Glucoamylase. IPR006425. Glucoamylase_bac. IPR015220. Glucodextran_N. IPR014718. Glyco_hydro-type_carb-bd_sub. IPR011613. Glyco_hydro_15. [Graphical view]
Pfam	PF09137. Glucodextran_N. 1 hit. PF00723. Glyco_hydro_15. 1 hit. [Graphical view]
SUPFAM	SSF74650. Gal_mut_like. 1 hit. SSF48208. Glyco_trans_6hp. 1 hit.
TIGRFAMs	TIGR01535. glucan_glucosid. 1 hit.
PROSITE	PS00820. GLUCOAMYLASE. 1 hit. PS51257. PROKAR_LIPOPROTEIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMYG_CLOS0

Accession

Primary (citable) accession number: P29761

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	April 3, 2013
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program