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P29761 (AMYG_CLOS0) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glucoamylase

EC=3.2.1.3
Alternative name(s):
1,4-alpha-D-glucan glucohydrolase
Glucan 1,4-alpha-glucosidase
Gene names
Name:cga
OrganismClostridium sp. (strain G0005)
Taxonomic identifier72582 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Protein attributes

Sequence length702 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

CGA has typical kinetic properties for a glucoamylase, but this bacterial enzyme had higher isomaltose-hydrolyzing activity than other eukaryotic glucoamylases.

Catalytic activity

Hydrolysis of terminal (1->4)-linked alpha-D-glucose residues successively from non-reducing ends of the chains with release of beta-D-glucose.

Subcellular location

Cell membrane; Lipid-anchor.

Miscellaneous

High activity towards 1,6-glycosidic bonds.

Sequence similarities

Belongs to the glycosyl hydrolase 15 family.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
Polysaccharide degradation
   Cellular componentCell membrane
Membrane
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   PTMLipoprotein
Palmitate
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological_processpolysaccharide catabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentplasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functioncarbohydrate binding

Inferred from electronic annotation. Source: InterPro

glucan 1,4-alpha-glucosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2121 Potential
Chain22 – 702681Glucoamylase
PRO_0000001481

Sites

Active site4521Proton acceptor By similarity
Active site4551Proton donor By similarity
Binding site3421Substrate By similarity

Amino acid modifications

Lipidation221N-palmitoyl cysteine Probable
Lipidation221S-diacylglycerol cysteine Probable

Sequences

Sequence LengthMass (Da)Tools
P29761 [UniParc].

Last modified April 1, 1993. Version 1.
Checksum: 955EB4D0AD569546

FASTA70278,658
        10         20         30         40         50         60 
MSRKLIKYLP LLVLASSVLS GCSNNVSSIK IDRFNNISAV NGPGEEDTWA SAQKQGVGTA 

        70         80         90        100        110        120 
NNYVSKVWFT LANGAISEVY YPTIDTADVK EIKFIVTDGK SFVSDETKDT ISKVEKFTDK 

       130        140        150        160        170        180 
SLGYKLVNTD KKGRYRITKE IFTDVKRNSL IMKAKFEALE GSIHDYKLYL AYDPHIKNQG 

       190        200        210        220        230        240 
SYNEGYVIKA NNNEMLMAKR DNVYTALSSN IGWKGYSIGY YKVNDIMTDL DENKQMTKHY 

       250        260        270        280        290        300 
DSARGNIIEG AEIDLKKNSQ FEIVLSFGNS EDEAVKASIE TLSENYDSLK SAYIDEWEKY 

       310        320        330        340        350        360 
CNSLNNFNGK ANSLYYNSMM ILKASEDKTN KGAYIASLSI PWGDGQGDDN TGGYHLVWSR 

       370        380        390        400        410        420 
DLYHVANAFI AAGDVDSANR SLDYLAKVVK DNGMIPQNTW ISGKPYWTGI QLDEQADPII 

       430        440        450        460        470        480 
LSYRLRRYDL YDSLVKPLAD FIIKMGPKTG QERWEEIGGY SPATMAAEVA GLTCAAYIAE 

       490        500        510        520        530        540 
QNKDYESAQK YQEKADNWQK LIDNLTYTEH GPLENGQYYI RIAGLPDPNA DFTISIANGG 

       550        560        570        580        590        600 
GVYDQKEIVD PSFLELVRLG VKSPDDPKIL NTLRVVDSTI KVDTPKGPSW YRYNHDGYGE 

       610        620        630        640        650        660 
PSKTELYHGA GKGRLWPLLT GERGMYEIAA GKDATPYLKA MENFANEGGI ISEQVWEDTG 

       670        680        690        700 
LPTDSASPLN WAHAEYVVLF PSNIEHKVLD MPDIVYKRYV AK 

« Hide

References

[1]"Molecular cloning of a glucoamylase gene from a thermophilic Clostridium and kinetics of the cloned enzyme."
Ohnishi H., Kitamura H., Minowa T., Sakai H., Ohta T.
Eur. J. Biochem. 207:413-418(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D12818 Genomic DNA. Translation: BAA02251.1.

3D structure databases

ProteinModelPortalP29761.
SMRP29761. Positions 28-701.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH15. Glycoside Hydrolase Family 15.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

SABIO-RKP29761.

Family and domain databases

Gene3D1.50.10.10. 1 hit.
2.70.98.10. 1 hit.
InterProIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR011013. Gal_mutarotase_SF_dom.
IPR000165. Glucoamylase.
IPR006425. Glucoamylase_bac.
IPR015220. Glucodextran_N.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR011613. Glyco_hydro_15.
[Graphical view]
PfamPF09137. Glucodextran_N. 1 hit.
PF00723. Glyco_hydro_15. 1 hit.
[Graphical view]
SUPFAMSSF48208. SSF48208. 1 hit.
SSF74650. SSF74650. 1 hit.
TIGRFAMsTIGR01535. glucan_glucosid. 1 hit.
PROSITEPS00820. GLUCOAMYLASE. 1 hit.
PS51257. PROKAR_LIPOPROTEIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameAMYG_CLOS0
AccessionPrimary (citable) accession number: P29761
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: October 16, 2013
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries