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P29760

- AMYI_YEASX

UniProt

P29760 - AMYI_YEASX

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Protein
Glucoamylase S2
Gene
STA2, DEX1
Organism
Saccharomyces cerevisiae (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal (1->4)-linked alpha-D-glucose residues successively from non-reducing ends of the chains with release of beta-D-glucose.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei456 – 4561Substrate By similarity
Active sitei519 – 5191Proton acceptor By similarity
Active sitei522 – 5221Proton donor By similarity

GO - Molecular functioni

  1. glucan 1,4-alpha-glucosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. polysaccharide catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Polysaccharide degradation

Protein family/group databases

CAZyiGH15. Glycoside Hydrolase Family 15.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucoamylase S2 (EC:3.2.1.3)
Alternative name(s):
1,4-alpha-D-glucan glucohydrolase
GAII
Glucan 1,4-alpha-glucosidase
Gene namesi
Name:STA2
Synonyms:DEX1
OrganismiSaccharomyces cerevisiae (Baker's yeast)
Taxonomic identifieri4932 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121
Add
BLAST
Chaini22 – 768747Glucoamylase S2
PRO_0000001478Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi35 – 351N-linked (GlcNAc...) Reviewed prediction
Glycosylationi309 – 3091N-linked (GlcNAc...) Reviewed prediction
Glycosylationi323 – 3231N-linked (GlcNAc...) Reviewed prediction
Glycosylationi415 – 4151N-linked (GlcNAc...) Reviewed prediction
Glycosylationi424 – 4241N-linked (GlcNAc...) Reviewed prediction
Glycosylationi435 – 4351N-linked (GlcNAc...) Reviewed prediction
Glycosylationi514 – 5141N-linked (GlcNAc...) Reviewed prediction
Glycosylationi547 – 5471N-linked (GlcNAc...) Reviewed prediction
Glycosylationi646 – 6461N-linked (GlcNAc...) Reviewed prediction
Glycosylationi651 – 6511N-linked (GlcNAc...) Reviewed prediction
Glycosylationi721 – 7211N-linked (GlcNAc...) Reviewed prediction
Glycosylationi742 – 7421N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Glycoprotein

Expressioni

Gene expression databases

GenevestigatoriP29760.

Structurei

3D structure databases

ProteinModelPortaliP29760.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni349 – 692344H subunit
Add
BLAST
Regioni693 – 76876Y subunit
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi22 – 348327Ser/Thr-rich
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di1.50.10.10. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR000165. Glucoamylase.
IPR011613. Glyco_hydro_15.
[Graphical view]
PfamiPF00723. Glyco_hydro_15. 1 hit.
[Graphical view]
PRINTSiPR00736. GLHYDRLASE15.
SUPFAMiSSF48208. SSF48208. 1 hit.
PROSITEiPS00820. GLUCOAMYLASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P29760-1 [UniParc]FASTAAdd to Basket

« Hide

MQRPFLLAYL VLSLLFNSAL GFPTALVPRG SSSSNITSSG PSSTPFSSAT    50
ESFSTGTTVT PSSSKYPGSK TETSVSSTTE TTIVPTTTTT SVITPSTTTI 100
TTTVCSTGTN SAGETTSGCS PKTITTTVPC STSPSETASE STTTSPTTPV 150
TTVVSTTVVT TEYASTSTKQ GGEITTTFVT KNIPTTYLTT IAPTSSVTTV 200
TNFTPTTITT TVCSTGTNSA GETTSGCSPK TVTTTVPCST GTGEYTTEAT 250
APVTTAVTTT VVTTESSTGT NSAGKTTTSY TTKSVPTTYV FDFGKGILDQ 300
SCGGVFSNNG SSQVQLRDVV LMNGTVVYDS NGAWDSSPLE EWLQRQKKVS 350
IERIFENIGP SAVYPSILPG VVIASPSQTH PDYFYQWIRD SALTINSIVS 400
HSADPAIETL LQYLNVSFHL QRTNNTLGAG IGYTNDTVAL GDPKWNVDNT 450
AFTEPWGRPQ NDGPALRSIA ILKIIDYIKQ SGTDLGAKYP FQSTADIFDD 500
IVRWDLRFII DHWNSSGFDL WEEVNGMHFF TLLVQLSAVD RSLSYFNASE 550
RSSPFVEELR QTRRDISKFL VDPANGFING KYNYIVETPM IADTLRSGLD 600
ISTLLAANTV HDAPSASHLP FDIDDPAVLN TLHHLMLHMR SIYPINDSSK 650
NATGIALGRY PEDVYDGYGV GEGNPWVLAT CAASTTLYQL IYRHISEQHD 700
LVVPMNNDCS NAFWSELVFS NLTTLGNDEG YLILEFNTPA FNQTIQKIFQ 750
LADSFLVKLK ATWEQTGN 768
Length:768
Mass (Da):82,587
Last modified:April 1, 1993 - v1
Checksum:i3FAC172C128A0C6F
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti164 – 1641Missing in AAA35107. 1 Publication
Sequence conflicti624 – 6241D → N in AAA35107. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M60650 Genomic DNA. Translation: AAA35107.1.
M90490 Genomic DNA. Translation: AAA20560.1.
PIRiJU0474.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M60650 Genomic DNA. Translation: AAA35107.1 .
M90490 Genomic DNA. Translation: AAA20560.1 .
PIRi JU0474.

3D structure databases

ProteinModelPortali P29760.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH15. Glycoside Hydrolase Family 15.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Gene expression databases

Genevestigatori P29760.

Family and domain databases

Gene3Di 1.50.10.10. 1 hit.
InterProi IPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR000165. Glucoamylase.
IPR011613. Glyco_hydro_15.
[Graphical view ]
Pfami PF00723. Glyco_hydro_15. 1 hit.
[Graphical view ]
PRINTSi PR00736. GLHYDRLASE15.
SUPFAMi SSF48208. SSF48208. 1 hit.
PROSITEi PS00820. GLUCOAMYLASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning of a new allelic variant of a Saccharomyces diastaticus glucoamylase gene and its introduction into industrial yeasts."
    Kim K., Bajszar G., Lee S.Y., Knudsen F., Mattoon J.R.
    Appl. Biochem. Biotechnol. 44:161-185(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Diastaticus.
  2. "Primary structure and regulation of a glucoamylase-encoding gene (STA2) in Saccharomyces diastaticus."
    Lambrechts M.G., Pretorius I.S., Sollitti P., Marmur J.
    Gene 100:95-103(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Diastaticus.

Entry informationi

Entry nameiAMYI_YEASX
AccessioniPrimary (citable) accession number: P29760
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: October 16, 2013
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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