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Reviewed, UniProtKB/Swiss-Prot P29760 (AMYI_YEAST)

Last modified October 13, 2009. Version 58. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Glucoamylase S2
    EC=3.2.1.3
Alternative name(s):
    Glucan 1,4-alpha-glucosidase
    1,4-alpha-D-glucan glucohydrolase
    GAII
Gene names
Name: STA2
Synonyms: DEX1
OrganismSaccharomyces cerevisiae (Baker's yeast)
Taxonomic identifier4932 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

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Protein attributes

Sequence length768 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology.

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General annotation (Comments)

Catalytic activity

Hydrolysis of terminal (1->4)-linked alpha-D-glucose residues successively from non-reducing ends of the chains with release of beta-D-glucose.

Sequence similarities

Belongs to the glycosyl hydrolase 15 family.

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Ontologies

Keywords
   Biological processCarbohydrate metabolism
Polysaccharide degradation
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   PTMGlycoprotein
Gene Ontology (GO)
   Biological processpolysaccharide catabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functionglucan 1,4-alpha-glucosidase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2121
Chain22 – 768747Glucoamylase S2
PRO_0000001478

Regions

Region349 – 692344H subunit
Region693 – 76876Y subunit
Compositional bias22 – 348327Ser/Thr-rich

Sites

Active site5191Proton acceptor By similarity
Active site5221Proton donor By similarity
Binding site4561Substrate By similarity

Amino acid modifications

Glycosylation351N-linked (GlcNAc...) Potential
Glycosylation3091N-linked (GlcNAc...) Potential
Glycosylation3231N-linked (GlcNAc...) Potential
Glycosylation4151N-linked (GlcNAc...) Potential
Glycosylation4241N-linked (GlcNAc...) Potential
Glycosylation4351N-linked (GlcNAc...) Potential
Glycosylation5141N-linked (GlcNAc...) Potential
Glycosylation5471N-linked (GlcNAc...) Potential
Glycosylation6461N-linked (GlcNAc...) Potential
Glycosylation6511N-linked (GlcNAc...) Potential
Glycosylation7211N-linked (GlcNAc...) Potential
Glycosylation7421N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict1641Missing in AAA35107. Ref.1
Sequence conflict6241D → N in AAA35107. Ref.1

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Sequences

Sequence LengthMass (Da)Tools
P29760-1 [UniParc].

Last modified April 1, 1993. Version 1.
Checksum: 3FAC172C128A0C6F

FASTA76882,587
        10         20         30         40         50         60 
MQRPFLLAYL VLSLLFNSAL GFPTALVPRG SSSSNITSSG PSSTPFSSAT ESFSTGTTVT 

        70         80         90        100        110        120 
PSSSKYPGSK TETSVSSTTE TTIVPTTTTT SVITPSTTTI TTTVCSTGTN SAGETTSGCS 

       130        140        150        160        170        180 
PKTITTTVPC STSPSETASE STTTSPTTPV TTVVSTTVVT TEYASTSTKQ GGEITTTFVT 

       190        200        210        220        230        240 
KNIPTTYLTT IAPTSSVTTV TNFTPTTITT TVCSTGTNSA GETTSGCSPK TVTTTVPCST 

       250        260        270        280        290        300 
GTGEYTTEAT APVTTAVTTT VVTTESSTGT NSAGKTTTSY TTKSVPTTYV FDFGKGILDQ 

       310        320        330        340        350        360 
SCGGVFSNNG SSQVQLRDVV LMNGTVVYDS NGAWDSSPLE EWLQRQKKVS IERIFENIGP 

       370        380        390        400        410        420 
SAVYPSILPG VVIASPSQTH PDYFYQWIRD SALTINSIVS HSADPAIETL LQYLNVSFHL 

       430        440        450        460        470        480 
QRTNNTLGAG IGYTNDTVAL GDPKWNVDNT AFTEPWGRPQ NDGPALRSIA ILKIIDYIKQ 

       490        500        510        520        530        540 
SGTDLGAKYP FQSTADIFDD IVRWDLRFII DHWNSSGFDL WEEVNGMHFF TLLVQLSAVD 

       550        560        570        580        590        600 
RSLSYFNASE RSSPFVEELR QTRRDISKFL VDPANGFING KYNYIVETPM IADTLRSGLD 

       610        620        630        640        650        660 
ISTLLAANTV HDAPSASHLP FDIDDPAVLN TLHHLMLHMR SIYPINDSSK NATGIALGRY 

       670        680        690        700        710        720 
PEDVYDGYGV GEGNPWVLAT CAASTTLYQL IYRHISEQHD LVVPMNNDCS NAFWSELVFS 

       730        740        750        760 
NLTTLGNDEG YLILEFNTPA FNQTIQKIFQ LADSFLVKLK ATWEQTGN

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References

[1]"Cloning of a new allelic variant of a Saccharomyces diastaticus glucoamylase gene and its introduction into industrial yeasts."
Kim K., Bajszar G., Lee S.Y., Knudsen F., Mattoon J.R.
Appl. Biochem. Biotechnol. 44:161-185(1994) [PubMed: 8017901] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Diastaticus.
[2]"Primary structure and regulation of a glucoamylase-encoding gene (STA2) in Saccharomyces diastaticus."
Lambrechts M.G., Pretorius I.S., Sollitti P., Marmur J.
Gene 100:95-103(1991) [PubMed: 2055484] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Diastaticus.

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Cross-references

Sequence databases

M60650 Genomic DNA. Translation: AAA35107.1.
M90490 Genomic DNA. Translation: AAA20560.1.
PIRJU0474.

3D structure databases

HSSPHSSP built from PDB template 1AYX based on UniProtKB P08017.
ModBaseSearch...

Protein-protein interaction databases

STRINGP29760.

Protein family/group databases

CAZyGH15. Glycoside Hydrolase Family 15.

Enzyme and pathway databases

BRENDA3.2.1.3. 250.

Gene expression databases

GenevestigatorP29760.

Family and domain databases

InterProIPR012341. 6hp_glycosidase.
IPR000165. Glyco_hydro_15.
IPR011613. Glyco_hydro_15_rel.
[Graphical view]
Gene3DG3DSA:1.50.10.10. CelA/Cel48F_cat. 1 hit.
PfamPF00723. Glyco_hydro_15. 1 hit.
[Graphical view]
PRINTSPR00736. GLHYDRLASE15.
PROSITEPS00820. GLUCOAMYLASE. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameAMYI_YEAST
AccessionPrimary (citable) accession number: P29760
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: October 13, 2009
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectFPAP (Fungal Proteome Annotation Project)

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Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents