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P29760

- AMYI_YEASX

UniProt

P29760 - AMYI_YEASX

Protein

Glucoamylase S2

Gene

STA2

Organism
Saccharomyces cerevisiae (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 76 (01 Oct 2014)
      Sequence version 1 (01 Apr 1993)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Hydrolysis of terminal (1->4)-linked alpha-D-glucose residues successively from non-reducing ends of the chains with release of beta-D-glucose.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei456 – 4561SubstrateBy similarity
    Active sitei519 – 5191Proton acceptorPROSITE-ProRule annotation
    Active sitei522 – 5221Proton donorPROSITE-ProRule annotation

    GO - Molecular functioni

    1. glucan 1,4-alpha-glucosidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. polysaccharide catabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Polysaccharide degradation

    Protein family/group databases

    CAZyiGH15. Glycoside Hydrolase Family 15.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glucoamylase S2 (EC:3.2.1.3)
    Alternative name(s):
    1,4-alpha-D-glucan glucohydrolase
    GAII
    Glucan 1,4-alpha-glucosidase
    Gene namesi
    Name:STA2
    Synonyms:DEX1
    OrganismiSaccharomyces cerevisiae (Baker's yeast)
    Taxonomic identifieri4932 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2121Add
    BLAST
    Chaini22 – 768747Glucoamylase S2PRO_0000001478Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi35 – 351N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi309 – 3091N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi323 – 3231N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi415 – 4151N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi424 – 4241N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi435 – 4351N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi514 – 5141N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi547 – 5471N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi646 – 6461N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi651 – 6511N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi721 – 7211N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi742 – 7421N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Expressioni

    Gene expression databases

    GenevestigatoriP29760.

    Structurei

    3D structure databases

    ProteinModelPortaliP29760.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni349 – 692344H subunitAdd
    BLAST
    Regioni693 – 76876Y subunitAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi22 – 348327Ser/Thr-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 15 family.Curated

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di1.50.10.10. 1 hit.
    InterProiIPR008928. 6-hairpin_glycosidase-like.
    IPR012341. 6hp_glycosidase.
    IPR000165. Glucoamylase.
    IPR011613. Glyco_hydro_15.
    [Graphical view]
    PfamiPF00723. Glyco_hydro_15. 1 hit.
    [Graphical view]
    PRINTSiPR00736. GLHYDRLASE15.
    SUPFAMiSSF48208. SSF48208. 1 hit.
    PROSITEiPS00820. GLUCOAMYLASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P29760-1 [UniParc]FASTAAdd to Basket

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    MQRPFLLAYL VLSLLFNSAL GFPTALVPRG SSSSNITSSG PSSTPFSSAT    50
    ESFSTGTTVT PSSSKYPGSK TETSVSSTTE TTIVPTTTTT SVITPSTTTI 100
    TTTVCSTGTN SAGETTSGCS PKTITTTVPC STSPSETASE STTTSPTTPV 150
    TTVVSTTVVT TEYASTSTKQ GGEITTTFVT KNIPTTYLTT IAPTSSVTTV 200
    TNFTPTTITT TVCSTGTNSA GETTSGCSPK TVTTTVPCST GTGEYTTEAT 250
    APVTTAVTTT VVTTESSTGT NSAGKTTTSY TTKSVPTTYV FDFGKGILDQ 300
    SCGGVFSNNG SSQVQLRDVV LMNGTVVYDS NGAWDSSPLE EWLQRQKKVS 350
    IERIFENIGP SAVYPSILPG VVIASPSQTH PDYFYQWIRD SALTINSIVS 400
    HSADPAIETL LQYLNVSFHL QRTNNTLGAG IGYTNDTVAL GDPKWNVDNT 450
    AFTEPWGRPQ NDGPALRSIA ILKIIDYIKQ SGTDLGAKYP FQSTADIFDD 500
    IVRWDLRFII DHWNSSGFDL WEEVNGMHFF TLLVQLSAVD RSLSYFNASE 550
    RSSPFVEELR QTRRDISKFL VDPANGFING KYNYIVETPM IADTLRSGLD 600
    ISTLLAANTV HDAPSASHLP FDIDDPAVLN TLHHLMLHMR SIYPINDSSK 650
    NATGIALGRY PEDVYDGYGV GEGNPWVLAT CAASTTLYQL IYRHISEQHD 700
    LVVPMNNDCS NAFWSELVFS NLTTLGNDEG YLILEFNTPA FNQTIQKIFQ 750
    LADSFLVKLK ATWEQTGN 768
    Length:768
    Mass (Da):82,587
    Last modified:April 1, 1993 - v1
    Checksum:i3FAC172C128A0C6F
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti164 – 1641Missing in AAA35107. (PubMed:8017901)Curated
    Sequence conflicti624 – 6241D → N in AAA35107. (PubMed:8017901)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M60650 Genomic DNA. Translation: AAA35107.1.
    M90490 Genomic DNA. Translation: AAA20560.1.
    PIRiJU0474.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M60650 Genomic DNA. Translation: AAA35107.1 .
    M90490 Genomic DNA. Translation: AAA20560.1 .
    PIRi JU0474.

    3D structure databases

    ProteinModelPortali P29760.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH15. Glycoside Hydrolase Family 15.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Gene expression databases

    Genevestigatori P29760.

    Family and domain databases

    Gene3Di 1.50.10.10. 1 hit.
    InterProi IPR008928. 6-hairpin_glycosidase-like.
    IPR012341. 6hp_glycosidase.
    IPR000165. Glucoamylase.
    IPR011613. Glyco_hydro_15.
    [Graphical view ]
    Pfami PF00723. Glyco_hydro_15. 1 hit.
    [Graphical view ]
    PRINTSi PR00736. GLHYDRLASE15.
    SUPFAMi SSF48208. SSF48208. 1 hit.
    PROSITEi PS00820. GLUCOAMYLASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning of a new allelic variant of a Saccharomyces diastaticus glucoamylase gene and its introduction into industrial yeasts."
      Kim K., Bajszar G., Lee S.Y., Knudsen F., Mattoon J.R.
      Appl. Biochem. Biotechnol. 44:161-185(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: Diastaticus.
    2. "Primary structure and regulation of a glucoamylase-encoding gene (STA2) in Saccharomyces diastaticus."
      Lambrechts M.G., Pretorius I.S., Sollitti P., Marmur J.
      Gene 100:95-103(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: Diastaticus.

    Entry informationi

    Entry nameiAMYI_YEASX
    AccessioniPrimary (citable) accession number: P29760
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1993
    Last sequence update: April 1, 1993
    Last modified: October 1, 2014
    This is version 76 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3