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P29760

- AMYI_YEASX

UniProt

P29760 - AMYI_YEASX

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Protein

Glucoamylase S2

Gene

STA2

Organism
Saccharomyces cerevisiae (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal (1->4)-linked alpha-D-glucose residues successively from non-reducing ends of the chains with release of beta-D-glucose.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei456 – 4561SubstrateBy similarity
Active sitei519 – 5191Proton acceptorPROSITE-ProRule annotation
Active sitei522 – 5221Proton donorPROSITE-ProRule annotation

GO - Molecular functioni

  1. glucan 1,4-alpha-glucosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. polysaccharide catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Polysaccharide degradation

Protein family/group databases

CAZyiGH15. Glycoside Hydrolase Family 15.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucoamylase S2 (EC:3.2.1.3)
Alternative name(s):
1,4-alpha-D-glucan glucohydrolase
GAII
Glucan 1,4-alpha-glucosidase
Gene namesi
Name:STA2
Synonyms:DEX1
OrganismiSaccharomyces cerevisiae (Baker's yeast)
Taxonomic identifieri4932 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121Add
BLAST
Chaini22 – 768747Glucoamylase S2PRO_0000001478Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi35 – 351N-linked (GlcNAc...)Sequence Analysis
Glycosylationi309 – 3091N-linked (GlcNAc...)Sequence Analysis
Glycosylationi323 – 3231N-linked (GlcNAc...)Sequence Analysis
Glycosylationi415 – 4151N-linked (GlcNAc...)Sequence Analysis
Glycosylationi424 – 4241N-linked (GlcNAc...)Sequence Analysis
Glycosylationi435 – 4351N-linked (GlcNAc...)Sequence Analysis
Glycosylationi514 – 5141N-linked (GlcNAc...)Sequence Analysis
Glycosylationi547 – 5471N-linked (GlcNAc...)Sequence Analysis
Glycosylationi646 – 6461N-linked (GlcNAc...)Sequence Analysis
Glycosylationi651 – 6511N-linked (GlcNAc...)Sequence Analysis
Glycosylationi721 – 7211N-linked (GlcNAc...)Sequence Analysis
Glycosylationi742 – 7421N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Expressioni

Gene expression databases

GenevestigatoriP29760.

Structurei

3D structure databases

ProteinModelPortaliP29760.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni349 – 692344H subunitAdd
BLAST
Regioni693 – 76876Y subunitAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi22 – 348327Ser/Thr-richAdd
BLAST

Sequence similaritiesi

Belongs to the glycosyl hydrolase 15 family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di1.50.10.10. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR000165. Glucoamylase.
IPR011613. Glyco_hydro_15.
[Graphical view]
PfamiPF00723. Glyco_hydro_15. 1 hit.
[Graphical view]
PRINTSiPR00736. GLHYDRLASE15.
SUPFAMiSSF48208. SSF48208. 1 hit.
PROSITEiPS00820. GLUCOAMYLASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P29760-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQRPFLLAYL VLSLLFNSAL GFPTALVPRG SSSSNITSSG PSSTPFSSAT
60 70 80 90 100
ESFSTGTTVT PSSSKYPGSK TETSVSSTTE TTIVPTTTTT SVITPSTTTI
110 120 130 140 150
TTTVCSTGTN SAGETTSGCS PKTITTTVPC STSPSETASE STTTSPTTPV
160 170 180 190 200
TTVVSTTVVT TEYASTSTKQ GGEITTTFVT KNIPTTYLTT IAPTSSVTTV
210 220 230 240 250
TNFTPTTITT TVCSTGTNSA GETTSGCSPK TVTTTVPCST GTGEYTTEAT
260 270 280 290 300
APVTTAVTTT VVTTESSTGT NSAGKTTTSY TTKSVPTTYV FDFGKGILDQ
310 320 330 340 350
SCGGVFSNNG SSQVQLRDVV LMNGTVVYDS NGAWDSSPLE EWLQRQKKVS
360 370 380 390 400
IERIFENIGP SAVYPSILPG VVIASPSQTH PDYFYQWIRD SALTINSIVS
410 420 430 440 450
HSADPAIETL LQYLNVSFHL QRTNNTLGAG IGYTNDTVAL GDPKWNVDNT
460 470 480 490 500
AFTEPWGRPQ NDGPALRSIA ILKIIDYIKQ SGTDLGAKYP FQSTADIFDD
510 520 530 540 550
IVRWDLRFII DHWNSSGFDL WEEVNGMHFF TLLVQLSAVD RSLSYFNASE
560 570 580 590 600
RSSPFVEELR QTRRDISKFL VDPANGFING KYNYIVETPM IADTLRSGLD
610 620 630 640 650
ISTLLAANTV HDAPSASHLP FDIDDPAVLN TLHHLMLHMR SIYPINDSSK
660 670 680 690 700
NATGIALGRY PEDVYDGYGV GEGNPWVLAT CAASTTLYQL IYRHISEQHD
710 720 730 740 750
LVVPMNNDCS NAFWSELVFS NLTTLGNDEG YLILEFNTPA FNQTIQKIFQ
760
LADSFLVKLK ATWEQTGN
Length:768
Mass (Da):82,587
Last modified:April 1, 1993 - v1
Checksum:i3FAC172C128A0C6F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti164 – 1641Missing in AAA35107. (PubMed:8017901)Curated
Sequence conflicti624 – 6241D → N in AAA35107. (PubMed:8017901)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M60650 Genomic DNA. Translation: AAA35107.1.
M90490 Genomic DNA. Translation: AAA20560.1.
PIRiJU0474.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M60650 Genomic DNA. Translation: AAA35107.1 .
M90490 Genomic DNA. Translation: AAA20560.1 .
PIRi JU0474.

3D structure databases

ProteinModelPortali P29760.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH15. Glycoside Hydrolase Family 15.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Gene expression databases

Genevestigatori P29760.

Family and domain databases

Gene3Di 1.50.10.10. 1 hit.
InterProi IPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR000165. Glucoamylase.
IPR011613. Glyco_hydro_15.
[Graphical view ]
Pfami PF00723. Glyco_hydro_15. 1 hit.
[Graphical view ]
PRINTSi PR00736. GLHYDRLASE15.
SUPFAMi SSF48208. SSF48208. 1 hit.
PROSITEi PS00820. GLUCOAMYLASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning of a new allelic variant of a Saccharomyces diastaticus glucoamylase gene and its introduction into industrial yeasts."
    Kim K., Bajszar G., Lee S.Y., Knudsen F., Mattoon J.R.
    Appl. Biochem. Biotechnol. 44:161-185(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Diastaticus.
  2. "Primary structure and regulation of a glucoamylase-encoding gene (STA2) in Saccharomyces diastaticus."
    Lambrechts M.G., Pretorius I.S., Sollitti P., Marmur J.
    Gene 100:95-103(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Diastaticus.

Entry informationi

Entry nameiAMYI_YEASX
AccessioniPrimary (citable) accession number: P29760
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: October 1, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3