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Protein

Glucoamylase S2

Gene

STA2

Organism
Saccharomyces cerevisiae (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal (1->4)-linked alpha-D-glucose residues successively from non-reducing ends of the chains with release of beta-D-glucose.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei456SubstrateBy similarity1
Active sitei519Proton acceptorPROSITE-ProRule annotation1
Active sitei522Proton donorPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Polysaccharide degradation

Protein family/group databases

CAZyiGH15. Glycoside Hydrolase Family 15.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucoamylase S2 (EC:3.2.1.3)
Alternative name(s):
1,4-alpha-D-glucan glucohydrolase
GAII
Glucan 1,4-alpha-glucosidase
Gene namesi
Name:STA2
Synonyms:DEX1
OrganismiSaccharomyces cerevisiae (Baker's yeast)
Taxonomic identifieri4932 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Add BLAST21
ChainiPRO_000000147822 – 768Glucoamylase S2Add BLAST747

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi35N-linked (GlcNAc...)Sequence analysis1
Glycosylationi309N-linked (GlcNAc...)Sequence analysis1
Glycosylationi323N-linked (GlcNAc...)Sequence analysis1
Glycosylationi415N-linked (GlcNAc...)Sequence analysis1
Glycosylationi424N-linked (GlcNAc...)Sequence analysis1
Glycosylationi435N-linked (GlcNAc...)Sequence analysis1
Glycosylationi514N-linked (GlcNAc...)Sequence analysis1
Glycosylationi547N-linked (GlcNAc...)Sequence analysis1
Glycosylationi646N-linked (GlcNAc...)Sequence analysis1
Glycosylationi651N-linked (GlcNAc...)Sequence analysis1
Glycosylationi721N-linked (GlcNAc...)Sequence analysis1
Glycosylationi742N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiP29760.

Interactioni

Protein-protein interaction databases

STRINGi4932.YIL099W.

Structurei

3D structure databases

ProteinModelPortaliP29760.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni349 – 692H subunitAdd BLAST344
Regioni693 – 768Y subunitAdd BLAST76

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi22 – 348Ser/Thr-richAdd BLAST327

Sequence similaritiesi

Belongs to the glycosyl hydrolase 15 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IGG8. Eukaryota.
COG3387. LUCA.

Family and domain databases

Gene3Di1.50.10.10. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR000165. Glucoamylase.
IPR011613. Glyco_hydro_15/PHK.
[Graphical view]
PfamiPF00723. Glyco_hydro_15. 1 hit.
[Graphical view]
PRINTSiPR00736. GLHYDRLASE15.
SUPFAMiSSF48208. SSF48208. 1 hit.
PROSITEiPS00820. GLUCOAMYLASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P29760-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQRPFLLAYL VLSLLFNSAL GFPTALVPRG SSSSNITSSG PSSTPFSSAT
60 70 80 90 100
ESFSTGTTVT PSSSKYPGSK TETSVSSTTE TTIVPTTTTT SVITPSTTTI
110 120 130 140 150
TTTVCSTGTN SAGETTSGCS PKTITTTVPC STSPSETASE STTTSPTTPV
160 170 180 190 200
TTVVSTTVVT TEYASTSTKQ GGEITTTFVT KNIPTTYLTT IAPTSSVTTV
210 220 230 240 250
TNFTPTTITT TVCSTGTNSA GETTSGCSPK TVTTTVPCST GTGEYTTEAT
260 270 280 290 300
APVTTAVTTT VVTTESSTGT NSAGKTTTSY TTKSVPTTYV FDFGKGILDQ
310 320 330 340 350
SCGGVFSNNG SSQVQLRDVV LMNGTVVYDS NGAWDSSPLE EWLQRQKKVS
360 370 380 390 400
IERIFENIGP SAVYPSILPG VVIASPSQTH PDYFYQWIRD SALTINSIVS
410 420 430 440 450
HSADPAIETL LQYLNVSFHL QRTNNTLGAG IGYTNDTVAL GDPKWNVDNT
460 470 480 490 500
AFTEPWGRPQ NDGPALRSIA ILKIIDYIKQ SGTDLGAKYP FQSTADIFDD
510 520 530 540 550
IVRWDLRFII DHWNSSGFDL WEEVNGMHFF TLLVQLSAVD RSLSYFNASE
560 570 580 590 600
RSSPFVEELR QTRRDISKFL VDPANGFING KYNYIVETPM IADTLRSGLD
610 620 630 640 650
ISTLLAANTV HDAPSASHLP FDIDDPAVLN TLHHLMLHMR SIYPINDSSK
660 670 680 690 700
NATGIALGRY PEDVYDGYGV GEGNPWVLAT CAASTTLYQL IYRHISEQHD
710 720 730 740 750
LVVPMNNDCS NAFWSELVFS NLTTLGNDEG YLILEFNTPA FNQTIQKIFQ
760
LADSFLVKLK ATWEQTGN
Length:768
Mass (Da):82,587
Last modified:April 1, 1993 - v1
Checksum:i3FAC172C128A0C6F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti164Missing in AAA35107 (PubMed:8017901).Curated1
Sequence conflicti624D → N in AAA35107 (PubMed:8017901).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M60650 Genomic DNA. Translation: AAA35107.1.
M90490 Genomic DNA. Translation: AAA20560.1.
PIRiJU0474.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M60650 Genomic DNA. Translation: AAA35107.1.
M90490 Genomic DNA. Translation: AAA20560.1.
PIRiJU0474.

3D structure databases

ProteinModelPortaliP29760.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi4932.YIL099W.

Protein family/group databases

CAZyiGH15. Glycoside Hydrolase Family 15.

Proteomic databases

PaxDbiP29760.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG410IGG8. Eukaryota.
COG3387. LUCA.

Family and domain databases

Gene3Di1.50.10.10. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR000165. Glucoamylase.
IPR011613. Glyco_hydro_15/PHK.
[Graphical view]
PfamiPF00723. Glyco_hydro_15. 1 hit.
[Graphical view]
PRINTSiPR00736. GLHYDRLASE15.
SUPFAMiSSF48208. SSF48208. 1 hit.
PROSITEiPS00820. GLUCOAMYLASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAMYI_YEASX
AccessioniPrimary (citable) accession number: P29760
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: November 11, 2015
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.